Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

HELLS px330

Expression vector of guide RNA for targeting human HELLS exon 19.

Catalog number RDB17381
Resource name HELLS px330
Alternative name HELLS exon19 pX330-U6-Chimeric_BB-CBh-hSpCas9
Clone info. Expression vector of guide RNA for targeting human helicase lymphoid specific (HELLS) exon 19 (5'-GAACCCCCAGTCGGATCTTC-3') and human codon-optimized SpCas9.
Vector backbone pX330-U6-Chimeric_BB-CBh-hSpCas9 (plasmid)
Size of vector backbone 8.5 kb
Selectable markers Amp^r (E. coli)
Gene/insert name human HELLS dsOligo
Depositor|Developer Unoki, Motoko |
Other clones in our bank
External Database
human HELLS
          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) and an acknowledgment to the DEPOSITOR are requested.
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          Exclusive MTA (For the DNA materials containing CRISPR/Cas9 technologies and for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks
          The BIOLOGICAL RESOURCE contains CRISPR/Cas9 technologies and is not provided to for-profit organization or for for-profit research by non-profit organizations.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Unoki, M et al. J. Clin. Invest. 129 (1), 78-92, 2019) を引用し、謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          専用MTA（CRISPR/Cas9内包遺伝子材料専用 非営利学術目的）をお使いください [Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考
          本件リソースはCRISPR/Cas9 technologyを用いたゲノム編集バイオリソースです。営利機関および非営利機関による営利目的研究には提供いたしません。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB17381 HELLS px330 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The HELLS px330 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17381).

          Reference section:

          Unoki, M., Funabiki, H., Velasco, G., Francastel, C., Sasaki, H., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17381_A9Fhp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pA3'element reverse (Pr0108)
          Region: U6 pro, gRNA (HELLS) gRNA scaffold, CBh pro 5'
          Sequence file: RDB17381_A9Fha.seq check
          >D06399A8_A9Fh_2_SV40pA3'element reverse_E03_15_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCNNGCAAC GCGGCCTTTT TACGGTTCCT
             61 GGCCTTTTGC TGGCCTTTTG CTCACATGTG AGGGCCTATT TCCCATGATT CCTTCATATT
            121 TGCATATACG ATACAAGGCT GTTAGAGAGA TAATTGGAAT TAATTTGACT GTAAACACAA
            181 AGATATTAGT ACAAAATACG TGACGTAGAA AGTAATAATT TCTTGGGTAG TTTGCAGTTT
            241 TAAAATTATG TTTTAAAATG GACTATCATA TGCTTACCGT AACTTGAAAG TATTTCGATT
            301 TCTTGGCTTT ATATATCTTG TGGAAAGGAC GAAACACCGA ACCCCCAGTC GGATCTTCGT
            361 TTTAGAGCTA GAAATAGCAA GTTAAAATAA GGCTAGTCCG TTATCAACTT GAAAAAGTGG
            421 CACCGAGTCG GTGCTTTTTT GTTTTAGAGC TAGAAATAGC AAGTTAAAAT AAGGCTAGTC
            481 CGTTTTTAGC GCGTGCGCCA ATTCTGCAGA CAAATGGCTC TAGAGGTACC CGTTACATAA
            541 CTTACGGTAA ATGGCCCGCC TGGCTGACCG CCCAACGACC CCCGCCCATT GACGTCAATA
            601 GTAACGCCAA TAGGGACTTT CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC
            661 CACTTGGCAG TACATCAAGT GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC
            721 GGTAAATGGC CCGCCTGGCA TTGTGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG
            781 CAGTACATCT ACGTATTAGT CATCGCTATT ACCATGGTCG AGGTGAGCCC CACGTTCTGC
            841 TTCACTCTCC CCATCTCCCC CCCCCTCCCC ACCCCCAANT TTGTATTTAT TTATTTTTTT
          901 NATTATTTTG TGCANCGATN GGGGGCGGGG GGGGGGGGGG GGGNN
          //
          Primer: Cas9-N_R1 (Pr0650)
          Region: Cas9 5', SV40 NLS, 3xFLAG, CBh pro 3'
          Sequence file: RDB17381_A9Fhb.seq check
          >D06399A8_A9Fh_2_Cas9-N_R1_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNTGNANT CGTCGGTGNN CACGGCCCNG CCCACAGAGT
             61 TGGTGCCGAT GTCCAGGCCG ATGCTGTACT TCTTGTCGGC TGCTGGGACT CCGTGGATAC
            121 CGACCTTCCG CTTCTTCTTT GGGGCCATCT TATCGTCATC GTCTTTGTAA TCAATATCAT
            181 GATCCTTGTA GTCTCCGTCG TGGTCCTTAT AGTCCATGGT GGCACCGGTC CAACCTGAAA
            241 AAAAGTGATT TCAGGCAGGT GCTCCAGGTA ATTAAACATT AATACCCCAC CAACCAACCA
            301 TCCCTTAAAC CCTTACCTCT TGCTCAGCTA ATTACAGCCC GGAGGAGAAG GGCCGTCCCG
          361 CCCGCTCACC TGTGGGAGTA ACGCGGTCAG TCAGAGCCGG GGCGGGCGGC GCGAGGNN
          //
          Primer: BGH_rev2 (Pr0606)
          Region: bGH p(A), nucleoplasmin NLS, Cas9 3'
          Sequence file: RDB17381_A9Fhc.seq check
          >D06399A8_A9Fh_2_BGH_rev2_E02_05_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NGGGGGNNGG GCNNACNACA GNNGGCTGGC AACTAGAAGG
             61 CACAGTCGAN GCTGATCAGC GAGCTCTAGG AATTCTTACT TTTTCTTTTT TGCCTGGCCG
            121 GCCTTTTTCG TGGCCGCCGG CCTTTTGTCG CCTCCCAGCT GAGACAGGTC GATCCGTGTC
            181 TCGTACAGGC CGGTGATGCT CTGGTGGATC AGGGTGGCGT CCAGCACCTC TTTGGTGCTG
            241 GTGTACCTCT TCCGGTCGAT GGTGGTGTCA AAGTACTTGA AGGCGGCAGG GGCTCCCAGA
            301 TTGGTCAGGG TAAACAGGTG GATGATATTC TCGGCCTGCT CTCTGATGGG CTTATCCCGG
            361 TGCTTGTTGT AGGCGGACAG CACTTTGTCC AGATTAGCGT CGGCCAGGAT CACTCTCTTG
            421 GAGAACTCGC TGATCTGCTC GATGATCTCG TCCAGGTAGT GCTTGTGCTG TTCCACAAAC
            481 AGCTGTTTCT GCTCATTATC CTCGGGGGAG CCCTTCAGCT TCTCATAGTG GCTGGCCAGG
            541 TACAGGAAGT TCACATATTT GGAGGGCAGG GCCAGTTCGT TTCCCTTCTG CAGTTCGCCG
            601 GCAGAGGCCA GCATTCTCTT CCGGCCGTTT TCCAGCTCGA ACAGGGAGTA CTTAGGCAGC
            661 TTGATGATCA GGTCCTTTTT CACTTCTTTG TAGCCCTTGG CTTCCAGAAA GTCGATGGGA
            721 NTCTTCTCGA AGCTGCTTCT TTCCATGATG GTGATCCCCA GCAGCTCTTT CACACTCTTC
            781 AGTTTCTTGG ACTTGCCCTT TTCCACTTTG GCCACCACCA GCACAGAATA GGCCACGGNN
            841 GGGCTGTCGA ANCCGCCGTA CTTCTNANGG TCCCANNCCN TCTTTCTGGC GATCAGCTTA
          901 TCGCNNTNCC NNTNGGGNAG GANANANTCN TNGCTGNNNN NNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Unoki, M., CDCA7 and HELLS mutations undermine nonhomologous end joining in centromeric instability syndrome. J. Clin. Invest. 129 (1): 78-92 (2019). PMID 30307408. [link to RRC of NBRP]

          2023.08.17

          GNP_filter3_RDBDEP_html_230702.pl