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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3.1 FLAG-hRFWD3 wt

Expression vector of human ring finger and WD repeat domain 3 (RFWD3) wild type.

Catalog number RDB17490
Resource name pcDNA3.1 FLAG-hRFWD3 wt
Clone info. Expression vector of human ring finger and WD repeat domain 3 (RFWD3) wild type, tagged with FLAG at N-terminus.
Comment Expression was confirmed by the depositor with immunoblotting. Known differences (at least): G to A substitution at 1269nt of NM_018124.4 (R392K; AGG to AAG).
Vector backbone pcDNA3.1/Zeo (plasmid)
Selectable markers E. coli, Ampicillin; mammalian cells, Zeocin.
Gene/insert name Human RFWD3 cDNA
Depositor|Developer Takata, Minoru |
Other clones in our bank

External Database
Human RFWD3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
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          Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
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          RDB17490 pcDNA3.1 FLAG-hRFWD3 wt DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17490_A9Iop1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro, FLAG, attB1, insert 5'
          Sequence file: RDB17490_A9Ioa.seq check
          >D06551A1_A9Io_2_CMV_Forward_C02_03_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGGGCGGT ANGCGTGTAC GGNGGGAGGT
             61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT
            121 TAATACGACT CACTATAGGG AGACCCAAGC TGGCTAGCAC CATGGATTAT AAAGATGATG
            181 ACGATAAGCA TGGTGGTTCT GGTCCAAGCT ATCAAACAAG TTTGTACAAA AAAGCAGGCT
            241 CCGCGGCCGC CCCCTTCACC ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT
            301 TAAATCATGC CGAACAACAG CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG
            361 CCCTCCTCCA GCCTGTTCCT GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC
            421 AGCCAGCTCC TGCTGAGGTG ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC
            481 CGCAACTGTC TGTTGACCTG ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA
            541 TCAATCCAAG AACTTCAGAA CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG
            601 CATCTTCGTT GCATTCAATG ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC
            661 TGGAATTCCT GAGACCTTCA TCTTCAAACC ACAGTGTANG GCCAATGAGA ACAAGAAGGA
            721 GGGTATCTGC TTCACGGAGG GCAAGAGCCG GANGGTCTCA GAGGANAGAC AGTGCCAGGT
            781 TGAGAGCACC ATTGGATGCT TACTTTCANG TGANCANGAC CCAGCCTGAN TTGCCAGCTA
            841 CCACTTATGA NTCNGAGACT NNGAATCCTG TATCTGAAGA GTTGCANGTG TCTAGTAGTT
            901 TNTGANTCTG ANAGTGNNAG CTCTGCNNAG TATGGANGGG TTGNNNANCN NGNCNNANGA
          961 ATCTGGANCT NNNNTTTNNN NNNNNNN
          //
          Primer: BGH_rev2
          Region: bGHp(A), attB2, insert 3'
          Sequence file: RDB17490_A9Iob.seq check
          >D06551A1_A9Io_2_BGH_rev2_B01_04_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NNGGGGGNNG GGCAAACAAC AGATGGCTGG CAACTAGAAG
             61 GCACAGTCGA GGCTGATCAG CGGGTTTATC GAACCACTTT GTACAAGAAA GCTGGGTCGG
            121 CGCGCCCACC CTTTCACTCC CACTTATAGA TGTGGACCAT CTTCTCTGTT AAGGTAGCCA
            181 AGTAGCTGTT ACGGTTCACC TCAAATGGGC AGATGTCCAA CACAGGCTGA TCGGTCTGTA
            241 GGTCCTGGAG CAACGAGCCA CTGGCAGCAT CCCACAGCAG GGCAGAATTT GCTGCTTCAT
            301 CCCCAGTACA CACCAGGATG TTGCCATCAT TCTCTGGGCT TTGGAAAATG GCATTTTTGG
            361 TCAATAGTTT GCAAGTAGGT CCTCCAAAAA ATGTATGTAC AGGCTGGCAG GAGCAGATTG
            421 GATTTCCAGT GTCATCCAGT CGGTAGGACA TTTCCATCAG CACACTTCGT ATGGTGGTGT
            481 GATTTTTATC AGGCCTGTAG GTCACAAGAC AGTGCCGGGA GCTGTTCTCT GTCTGAAAGT
            541 CTATGCAGCC CCCTGGCTCC AAGGGCAGCA CATGAGGCCA ATGAGAAAAG TCCATTTTCT
            601 GTTCCCAGAA TGAAGCATCC TCCAAGGTTC CAGCCAGCAC CCCACCATAT GGAAATGCAG
            661 CTGAGGCAGC TCTGGGCATG TATGACAGGG AGACCAGTGG GCATCTGGCT TTCTGAGCTA
            721 CTAACTCCTG CACATGACTG CTCGTGTTTC GCACGTCATA TACCAGAATT GAACCATTGG
            781 CCAGTCCAGC ATAGATGTAG TTAGCCTCAT CAAGACACCA GCAACAGCTC CAGACAGGAC
            841 GTCCAGCATT ATAAGTCTGG ACCACGGTAT TTGTCTCCNG GCTGGTCAGT TTAATAGTGT
            901 TGTCTAGGGA AGCANANAGT ANCANGCCTC TGANGNNACT GCTNAACGCC AGNCCACGGA
            961 TCTGTTTGNC NTGCATNGGA ATGTACTGNC TGNNCTTCAT GTTGGCAGTN NNCNNNNTCN
          1021 TNACNNNNAA NNCNNNNNNN AAAANNNNCN NNNNNNNNN
          //
          Primer: hRFWD3-N_F
          Region: insert mid
          Sequence file: RDB17490_A9Ioc.seq check
          >D06551A1_A9Io_2_hRFWD3-N_F_A01_01_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNTCTGNNAN TTGCAGGTGT CTAGTAGTTC TGATTCTGAC
             61 AGTGACAGCT CTGCAGAGTA TGGAGGGGTT GTTGACCAGG CAGAGGAATC TGGAGCTGTC
            121 ATTTTAGAAG AGCAACTAGC AGGTGTCTCA GCAGAGCAAG AAGTTACATG TATCGATGGA
            181 GGCAAGACCC TCCCCAAACA GCCATCTCCC CAGAAGTCTG AGCCTCTGCT ACCTTCTGCT
            241 TCTATGGATG AGGAAGAAGG GGACACTTGT ACAATATGTC TGGAACAGTG GACCAATGCT
            301 GGGGACCACC GGCTCTCAGC ATTACGCTGT GGGCATCTCT TTGGGTATAG GTGCATTTCC
            361 ACGTGGCTTA AAGGACAAGT ACGAAAATGT CCCCAGTGCA ACAAGAAAGC CAGGCACAGT
            421 GACATTGTCG TCCTTTATGC CCGAACCCTG AGAGCTTTGG ACACTAGTGA ACAGGAGCGC
            481 ATGAAAAGTT CCCTACTGAA GGAACAGATG CTAAGGAAAC AGGCCGAGTT AGAATCAGCA
            541 CAGTGCCGAC TCCAACTGCA GGTCCTCACT GATAAGTGCA CTAAGCTTCA AAGGCGTGTT
            601 CAGGACTTGC AAAAACTTAC GTCACATCAA AGTCAGAATT TACAGCAACC CAGGGGCTCC
            661 CAAGCATGGG TCCTGAGCTG CTCACCCTCC AGCCAGGGCC AGCACAAGCA CAAGTACCAC
            721 TTCCAAAAGA CCTTCACAGT ATCTCAGGCA GGAAACTGCC GGATCATGGC ATACTGTGAT
            781 GCTCTGAGCT GCCTGGTGAT ATCACAGCCT TCTCCTCANG CCTCTTTTCT TCCNGGCTTT
            841 NGTGTTAAGA TGTTGAGTAC TGCCAACATG AAGAGCAGTC AGTACATTCC GATGCATGGC
            901 AAACAGATNC GNNGANNGGC GTTTANCAGT TACCNCANAN GNTTGCTANT CTCNGCTTCC
          961 NNANNNNNCA NTATTAAACT GANNANNCNN NNNNNNNN
          //
          Primer: SV40pro_ori_F
          Region: SV40pro_ori, bleoR, SV40p(A)
          Sequence file: RDB17490_A9Iod.seq check
          >D06551A1_A9Io_2_SV40pro_ori_F_C01_07_ABI24.ab1
              1 NNNNNNNNNN NNNNGNCGCC TCTGCCTCTG ANCTNTTCNN GAAGTAGTGA GGAGGCTTTT
             61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG
            121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA
            181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA
            241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA
            301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA
            361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA
            421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC
            481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG
            541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC
            601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT
            661 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA
            721 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT
            781 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC
            841 GACCTCTANC TAGAGCTTGG CGTAATCATG GNCATAGCTG TNTNCTGTGT GAAATTGTTA
            901 TCCGCNCNCN ATNNCNCACN ANATACNANC CNGNNNCATN AAGTGTAAAG NCTGGGGNGN
          961 NNNANGANTG ANCTNNNNCN NATTNANTNN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Inano, S., RFWD3-Mediated Ubiquitination Promotes Timely Removal of Both RPA and RAD51 from DNA Damage Sites to Facilitate Homologous Recombination. Mol. Cell 66 (5): 622-634.e8 (2017). PMID 28575658.

          2022.05.17

          GNP_filter3_RDBDEP_html_220516.pl