Please review the QC test results indicated by icon below as well as clone information before placing your order.

pcDNA3.1 FLAG-hRFWD3 I639K

Expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, I639K, chromatin localization-defective mutation.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17492_A9Iop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward Region: CMV pro, FLAG, attB1, insert 5' Sequence file: RDB17492_A9Ioa.seq
>D06551A3_A9Io_2_CMV_Forward_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGNNGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT    61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT   121 TAATACGACT CACTATAGGG AGACCCAAGC TGGCTAGCAC CATGGATTAT AAAGATGATG   181 ACGATAAGCA TGGTGGTTCT GGTCCAAGCT ATCAAACAAG TTTGTACAAA AAAGCAGGCT   241 CCGCGGCCGC CCCCTTCACC ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT   301 TAAATCATGC CGAACAACAG CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG   361 CCCTCCTCCA GCCTGTTCCT GCTGATGTGG TCAGCAGCCA GGGGGTACCA TCCATCCTCC   421 AGCCAGCTCC TGCTGAGGTG ATCAGCAGCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC   481 CGCAACTGTC TGTTGACCTG ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA   541 TCAATCCAAG AACTTCAGAA CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG   601 CATCTTCGTT GCATTCAATG ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC   661 TGGAATTCCT GAGACCTTCA TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA   721 NGGTATCTGC TTCACGGAGG GCAAGAGCCG GAGGGTCTCA GANGACAGAC AGTGCCAGGT   781 TGAGAGCACC ATTGGATGCT TACTTTCANG TGAGCAGGAN CCAGCCTGAC TTGCCAGCTA   841 CCACTTATGA TTCAGAGANT AGGAATCCTG TATCTGAAGA NTTGCAGGTG TCTAGTAGTT   901 CTGATTCTGA NAGTGACAGC TCTGCANANT ATNNAGGGGT TGTTGNNNNN NNANANGAAT   961 CTGGANCTGN CATTTTANAA GANCNNCNNG CANNNGNCNC ANCNNANCNN GNANNTTACA 1021 TNNNTCNNNG NNNNNNNNNN //
Primer: f1ori_R3 Region: bGHp(A), attB2, insert 3' (I639K) Sequence file: RDB17492_A9Iob.seq
>D06551A3_A9Io_2_f1ori_R3_C03_09_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNGNNCTTT CNGCCTNNGA AGCCNTAGAG CCCACCGCAT    61 CCCCAGCATG CCTGCTATTG TCTTCCCAAT CCTCCCCCTT GCTGTCCTGC CCCACCCCAC   121 CCCCCAGAAT AGAATGACAC CTACTCAGAC AATGCGATGC AATTTCCTCA TTTTATTAGG   181 AAAGGACAGT GGGAGTGGCA CCTTCCAGGG TCAAGGAAGG CACGGGGGAG GGGCAAACAA   241 CAGATGGCTG GCAACTAGAA GGCACAGTCG AGGCTGATCA GCGGGTTTAT CGAACCACTT   301 TGTACAAGAA AGCTGGGTCG GCGCGCCCAC CCTTTCACTC CCACTTATAG ATGTGGACCA   361 TCTTCTCTGT TAAGGTAGCC AAGTAGCTGT TACGGTTCAC CTCAAATGGG CAGATGTCCA   421 ACACAGGCTG ATCGGTCTGT AGGTCCTGGA GCAACGAGCC ACTGGCAGCA TCCCACAGCA   481 GGGCAGAATT TGCTGCTTCA TCCCCAGTAC ACACCAGGAT GTTGCCATCA TTCTCTGGGC   541 TTTGGAAAAT GGCATTTTTG GTCAATAGTT TGCAAGTAGG TCCTCCAAAA AATGTATGTA   601 CAGGCTGGCA GGAGCAGATT GGATTTCCAG TGTCATCCAG TCGGTAGGAC ATTTCCATCA   661 GCACACTTCG TATGGTGGTG TGATTTTTAT CAGGCCTGTA GGTCACAAGA CAGTGCCGGG   721 AGCTGTTCTC TGTCTGAAAG TCTTTGCACC CCCCTGGCTC CAAGGGCAGC ACATGAGGCC   781 AATGAGAAAA GTCCATTTTC TGTTCCCAGA ATGAAGCATC CTCCAAGGTT CCAGCCAGCA   841 CCCCACCATA TGGAAATGCA GCTGANGCAG CTCTGGGCAT GTATGACAGG GANACCAGNG   901 GGCATCTGNC TTTCTGANCT ACTAACTCCT GCNCATGACT GCNCNNGTTT CNCANGNCAT   961 ATNCCAGANN TGANNNATNG GNNAGTCCAN CATANNTGTA NTNAGNCNCA TNNNNNNNNC 1021 NNCNNCNNNN NNNNNN //
Primer: hRFWD3-N_F Region: insert mid Sequence file: RDB17492_A9Ioc.seq
>D06551A3_A9Io_2_hRFWD3-N_F_B04_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNAT CTGANANTTG CAGGTGTCTA GTAGTTCTGA TTCTGACAGT    61 GACAGCTCTG CAGAGTATGG AGGGGTTGTT GACCAGGCAG AGGAATCTGG AGCTGTCATT   121 TTAGAAGAGC AACTAGCAGG TGTCTCAGCA GAGCAAGAAG TTACATGTAT CGATGGAGGC   181 AAGACCCTCC CCAAACAGCC ATCTCCCCAG AAGTCTGAGC CTCTGCTACC TTCTGCTTCT   241 ATGGATGAGG AAGAAGGGGA CACTTGTACA ATATGTCTGG AACAGTGGAC CAATGCTGGG   301 GACCACCGGC TCTCAGCATT ACGCTGTGGG CATCTCTTTG GGTATAGGTG CATTTCCACG   361 TGGCTTAAAG GACAAGTACG AAAATGTCCC CAGTGCAACA AGAAAGCCAG GCACAGTGAC   421 ATTGTCGTCC TTTATGCCCG AACCCTGAGA GCTTTGGACA CTAGTGAACA GGAGCGCATG   481 AAAAGTTCCC TACTGAAGGA ACAGATGCTA AGGAAACAGG CCGAGTTAGA ATCAGCACAG   541 TGCCGACTCC AACTGCAGGT CCTCACTGAT AAGTGCACTA AGCTTCAAAG GCGTGTTCAG   601 GACTTGCAAA AACTTACGTC ACATCAAAGT CAGAATTTAC AGCAACCCAG GGGCTCCCAA   661 GCATGGGTCC TGAGCTGCTC ACCCTCCAGC CAGGGCCAGC ACAAGCACAA GTACCACTTC   721 CAAAAGANCT TCACAGTATC TCAGGCAGGA AACTGCCGGA TCATGGCATA CTGTGATGCT   781 CTGANCTGCC TGGTGATATC ACAGCCTTCT CCTCANGCCT CTTTTCTTCC AGGCTTTGGT   841 GTTAAGANGT TGAGTACTGC CAACATGAAG AGCAGTCAGT ACATTCCGAT GCNTGGCAAA   901 CAGATCCGNN NANNGGCGTT TANCAGTTAN CTCANANGNT TGCTACTCTC TGCTTCCCTA 961 NANNACACTA TTAAACNGAN NNNCCNNNNN NNNAANANNN NNNNNNN //
Primer: SV40pro_ori_F Region: SV40pro_ori, bleoR, SV40p(A) Sequence file: RDB17492_A9Iod.seq
>D06551A3_A9Io_2_SV40pro_ori_F_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCGCCTCTGC CTCTGANCTA TTCNNNAAGT AGTGAGGAGG    61 CTTTTTTGGA GGCCTAGGCT TTTGCAAAAA GCTCCCGGGA GCTTGTATAT CCATTTTCGG   121 ATCTGATCAG CACGTGTTGA CAATTAATCA TCGGCATAGT ATATCGGCAT AGTATAATAC   181 GACAAGGTGA GGAACTAAAC CATGGCCAAG TTGACCAGTG CCGTTCCGGT GCTCACCGCG   241 CGCGACGTCG CCGGAGCGGT CGAGTTCTGG ACCGACCGGC TCGGGTTCTC CCGGGACTTC   301 GTGGAGGACG ACTTCGCCGG TGTGGTCCGG GACGACGTGA CCCTGTTCAT CAGCGCGGTC   361 CAGGACCAGG TGGTGCCGGA CAACACCCTG GCCTGGGTGT GGGTGCGCGG CCTGGACGAG   421 CTGTACGCCG AGTGGTCGGA GGTCGTGTCC ACGAACTTCC GGGACGCCTC CGGGCCGGCC   481 ATGACCGAGA TCGGCGAGCA GCCGTGGGGG CGGGAGTTCG CCCTGCGCGA CCCGGCCGGC   541 AACTGCGTGC ACTTCGTGGC CGAGGAGCAG GACTGACACG TGCTACGAGA TTTCGATTCC   601 ACCGCCGCCT TCTATGAAAG GTTGGGCTTC GGAATCGTTT TCCGGGACGC CGGCTGGATG   661 ATCCTCCAGC GCGGGGATCT CATGCTGGAG TTCTTCGCCC ACCCCAACTT GTTTATTGCA   721 GCTTATAATG GTTACAAATA AAGCAATAGC ATCACAAATT TCACAAATAA AGCATTTTTT   781 TCACTGCATT CTAGTTGTGG TTTGTCCAAA CTCATCAATG TATCTTATCA TGTCTGTATA   841 CCGTCGANCT CTANCTAGAG CTTGGCGTAA TCATGGTCAT ANCTGTTTNC TGTGTGAAAT   901 TGTNATCCGC TCACNATTNN NCACAACATA CGAGCCNGNA AGCATAAAGT GNAANNCNGG   961 GGGNGNCNNA TGAGTGANCT AACNCACATT ANTTGNNTTG CGCNCNNNGN CNNCTTTNCA 1021 GNNNGNNANN NNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl