Please review the QC test results indicated by icon below as well as clone information before placing your order.

CSII FLAG-hRFWD3 S46/63A

Lentivirus expression vector of human ring finger and WD repeat domain 3 (RFWD3) mutant, S46/63A, non-phosphorylatable mutation.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Additional terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose. The RECIPIENT agrees to expressly describe the late Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector.
Ordering	Please visit Ordering instruction.[link]  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA, for use for not-for-profit academic purpose)[Word]
Remarks	Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
付加的提供条件	本件研究材料は、非営利機関の非営利学術研究に限って提供する。レンチウイルスベクターについて、本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが故三好浩之博士により開発されたことを明示する。
提供依頼	手続きの詳細は、「レンチウイルスベクターの提供申し込み」をご覧ください。 提供依頼書 [Word] 提供同意書 (非営利機関による非営利学術研究用)[Word] 遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。
備考	Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17499_A9Iop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: hRFWD3-N_R Region: insert 5'(S46A), attB1, FLAG, CMV pro 2, CMV en 2 Sequence file: RDB17499_A9Ioa.seq
>D06551B1_A9Io_2_hRFWD3-N_R_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNC TNNGCNNGNN CTGGCTGGNN GANGGATGGT    61 ACCCCCTGGG CGCTGACCAC ATCAGCAGGA ACAGGCTGGA GGAGGGCTGG TCCCCCTTGG   121 CTGCTGGCCA TGCCAGCAGG AGCTGGCTGT TGTTCGGCAT GATTTAACTG CACCTGAACA   181 TCATATTCCA TTGCTTCATG AGCCATGGTG AAGGGGGCGG CCGCGGAGCC TGCTTTTTTG   241 TACAAACTTG TTTGATAGCT TGGACCAGAA CCACCATGCT TATCGTCATC ATCTTTATAA   301 TCCATGGTGC TAGCCAGCTT GGGTCTCCCT AGCGGATCTG ACGGTTCACT AAACCAGCTC   361 TGCTTATATA GACCTCCCAC CGTACACGCC TACCGCCCAT TTGCGTCAAT GGGGCGGAGT   421 TGTTACGACA TTTTGGAAAG TCCCGTTGAT TTTGGTGCCA AAACAAACTC CCATTGACGT   481 CAATGGGGTG GAGACTTGGA AATCCCCGTG AGTCAAACCG CTATCCACGC CCATTGATGT   541 ACTGCCAAAA CCGCATCACC ATGGTAATAG CGATGACTAA TACGTAGATG TACTGCCAAG   601 TAGGAAAGTC CCATAAGGTC ATGTACTGGG CATAATGCCA GGCGGGCCAT TTACCGTCAT   661 TGACGTCAAT AGGGGGCGTA CTTGGCATAT GATACACTTG ATGTACTGCC AAGTGGGCAG   721 TTTACCGTAA ATACTCCACC CATTGACGTC AATGGAAAGT CCCTATTGGC GTTACTATGG   781 GAACATACGT CATTATTGAC GTCAATGGGC GGGGGTCGTT GGGCGGTCAG CCAGGCGGGC   841 CATTTACCGT AAGTTATGTA ACGCGGAACT CCATATATGG GCTATGAACT AATGANCCNG   901 TAATTGATTA CTATTAATCG AANNNATTNN AAGTGAATTN CTGCTGNCNN TGNNNTAAAN   961 CCGAAAATTT NNAANTTTTN GTNNNTTGNT TTTGNNANTN NNTTANTTNG NANNNNNNNN 1021 NN //
Primer: IRES_R3 Region: attB2, insert 3' Sequence file: RDB17499_A9Iob.seq
>D06551B1_A9Io_2_IRES_R3_E06_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNNGCGGNN CCGCGGCCGC TGCNNAATTC    61 TCGATGGCAA CTAGAAGGCA CAGTCGAGGC TGATCAGCGG GTTTATCGAA CCACTTTGTA   121 CAAGAAAGCT GGGTCGGCGC GCCCACCCTT TCACTCCCAC TTATAGATGT GGACCATCTT   181 CTCTGTTAAG GTAGCCAAGT AGCTGTTACG GTTCACCTCA AATGGGCAGA TGTCCAACAC   241 AGGCTGATCG GTCTGTAGGT CCTGGAGCAA CGAGCCACTG GCAGCATCCC ACAGCAGGGC   301 AGAATTTGCT GCTTCATCCC CAGTACACAC CAGGATGTTG CCATCATTCT CTGGGCTTTG   361 GAAAATGGCA TTTTTGGTCA ATAGTTTGCA AGTAGGTCCT CCAAAAAATG TATGTACAGG   421 CTGGCAGGAG CAGATTGGAT TTCCAGTGTC ATCCAGTCGG TAGGACATTT CCATCAGCAC   481 ACTTCGTATG GTGGTGTGAT TTTTATCAGG CCTGTAGGTC ACAAGACAGT GCCGGGAGCT   541 GTTCTCTGTC TGAAAGTCTA TGCAGCCCCC TGGCTCCAAG GGCAGCACAT GAGGCCAATG   601 AGAAAAGTCC ATTTTCTGTT CCCAGAATGA AGCATCCTCC AAGGTTCCAG CCAGCACCCC   661 ACCATATGGA AATGCAGCTG AGGCAGCTCT GGGCATGTAT GACAGGGAGA CCAGTGGGCA   721 TCTGGCTTTC TGANCTACTA ACTCCTGCAC ATGACTGCTC GTGTTTCGCA CGTCNTATAC   781 CANANTTGAA CCATTGGCCA GTCCANNATA GATGTNNNTA NCCTCATCAN NACNCCAGCA   841 ACAGCTCCNG ACAGGACGTN CAGCATTANN AAGTCTGGAN CNCGGTATTT GTCTCCNNGC   901 TGGTCAGNTN NATANNGTTG TCTAGGGNAN CANANAGTAG NNAGCCTCTG ANGNANNNGC   961 NNAACGCCNN NNCNCGGATC TGTTTNNCNT GCNNCGGAAN TGNNCTGNNN GNNNNTNNNN 1021 NNNGNCAGNN NNNNNNNTNN TNNNNNNNNN AANNNNNNNN NN //
Primer: CSII_CMV5_v2 Region: FLAG, attB1, insert 5' (S46A, S63A) Sequence file: RDB17499_A9Ioc.seq
>D06551B1_A9Io_2_CSII_CMV5_v2_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGGCNNNCNN CNTGGNNTAT AAAGANGATG ACGATAAGCA    61 TGGTGGTTCT GGTCCAAGCT ATCAAACAAG TTTGTACAAA AAAGCAGGCT CCGCGGCCGC   121 CCCCTTCACC ATGGCTCATG AAGCAATGGA ATATGATGTT CAGGTGCAGT TAAATCATGC   181 CGAACAACAG CCAGCTCCTG CTGGCATGGC CAGCAGCCAA GGGGGACCAG CCCTCCTCCA   241 GCCTGTTCCT GCTGATGTGG TCAGCGCCCA GGGGGTACCA TCCATCCTCC AGCCAGCTCC   301 TGCTGAGGTG ATCAGCGCCC AAGCGACACC ACCCCTGCTC CAGCCTGCTC CGCAACTGTC   361 TGTTGACCTG ACAGAAGTGG AGGTCTTGGG AGAAGACACT GTGGAGAACA TCAATCCAAG   421 AACTTCAGAA CAACATAGGC AGGGATCTGA TGGTAATCAC ACCATCCCAG CATCTTCGTT   481 GCATTCAATG ACCAACTTCA TCAGCGGACT GCAGAGACTT CATGGCATGC TGGAATTCCT   541 GAGACCTTCA TCTTCAAACC ACAGTGTAGG GCCAATGAGA ACAAGAAGGA GGGTATCTGC   601 TTCACGGAGG GCAAGAGCCG GAGGGTCTCA GAGGACAGAC AGTGCCAGGT TGAGAGCACC   661 ATTGGATGCT TACTTTCAGG TGAGCAGGAC CCAGCCTGAC TTGCCAGCTA CCACTTATGA   721 TTCAGAGACT AGGAATCCTG TATCTGAAGA GTTGCAGGTG TCTAGTAGTT CTGATTCTGA   781 CAGTGACAGC TCTGCAGANT ATGGAGGGGT TGTTGANCAG GCAGANGAAT CTGGANCTGT   841 CATTTTAGAA GANCAACTAG CANGTGTCTC AGCAGANCAA GAAGTTNCAT GTATCGATGG   901 ANGCAAGANC NNCCCCAAAC AGCCATCTCC CCANAANNNT GANCCNCTGC NANCTTCTGC   961 TTCTATGGAT GANGNAAGNN NNGGNANNNN TGNNNNNTAT GTCNNGNAAN NGNNNNNNNA 1021 NNNNNNGGGN NNNNNNNNN //
Primer: HIVgp_R1 Region: HIV-1 psi, 5' LTR Sequence file: RDB17499_A9Iod.seq
>D06551B1_A9Io_2_HIVgp_R1_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGC TCTTTTTTCT CTCTGCNCNN CTCTTCTCTT TGCCTTGGTG    61 GGTGCTACTC CTAATGGTTC AATTTTTACT ACTTTATATT TATATAATTC ACTTCTCCAA   121 TTGTCCCTCA TATCTCCTCC TCCAGGTCTG AAGATCAGCG GCCGGCCGCT TGCTGTGCGG   181 TGGTCTTACT TTTGTTTTGC TCTTCCTCTA TCTTGTCTAA AGCTTCCTTG GTGTCTTTTA   241 TCTCTATCCT TTGATGCACA CAATAGAGGG TTGCTACTGT ATTATATAAT GATCTAAGTT   301 CTTCTGATCC TGTCTGAAGG GATGGTTGTA GCTGTCCCAG TATTTGTCTA CAGCCTTCTG   361 ATGTTTCTAA CAGGCCAGGA TTAACTGCGA ATCGTTCTAG CTCCCTGCTT GCCCATACTA   421 TATGTTTTAA TTTATATTTT TTCTTTCCCC CTGGCCTTAA CCGAATTTTT TCCCATCGCG   481 ATCTAATTCT CCCCCGCTTA ATACTGACGC TCTCGCACCC ATCTCTCTCC TTCTAGCCTC   541 CGCTAGTCAA AATTTTTGGC GTACTCACCA GTCGCCGCCC CTCGCCTCTT GCCGTGCGCG   601 CTTCAGCAAG CCGAGTCCTG CGTCGAGAGA GCTCCTCTGG TTTCCCTTTC GCTTTCAAGT   661 CCCTGTTCGG GCGCCACTGC TAGAGATTTT CCACACTGAC TAAAAGGGTC TGANGGATCT   721 CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT TGAAGCACTC AAGGCAAGCT   781 TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAN AGCTCCCANG CTCAGATCTG   841 GTCTAACCAG AGAGACCCAG TACANGCAAA ACGCGCTGCT TATATAGACC TCCNNCCGTA   901 CNCGCCTACN GCCNNTTTGC GTCAATGGGG GCGGANTTGT NNCNANATTT TGGAAANTCC   961 CNNNGANTTT GGTGNCNAAA NNAACTCCCN NNGNNNNNNN NGGGGNGGNN NNTNNNNNAN 1021 NCCCNNNNNN TCNANNNNNN NNNN //
Primer: cPPT+CTS_v2_F Region: cPPT_CTS, CMV en 2, CMV pro 2 Sequence file: RDB17499_A9Ioe.seq
>D06551B1_A9Io_2_cPPT+CTS_v2_F_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNANNAATT ACAAAAATTC AAAATTTTCG    61 GGTTTATTAC AGGGACAGCA GAAATTCACT TTGAATTAAT TCGATTAATA GTAATCAATT   121 ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT   181 GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT   241 CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA   301 ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA GTACGCCCCC TATTGACGTC   361 AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA TGACCTTATG GGACTTTCCT   421 ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG   481 TACATCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT   541 GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAC   601 AACTCCGCCC CATTGACGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC   661 AGAGCTGGTT TAGTGAACCN NNNNATCCGC TAGGGAGACC CAAGCTGGCT AGCACCATGG   721 ATTATAAAGA TGATGACGAT AAGCATGGTG GTTCTGGTCC AAGCTATCNN ACAAGTTTGT   781 ACAAAAAAGC AGGCTCCGCG GCCGCCCCCT TCACCATGGC TCATGAAGCA ATGNAATATG   841 ATGNTCAGGT GCAGTTAAAT CATGCCNAAC NACAGCCAGC TNCNGCTGGC ATGGNCAGCA   901 GNNNANGGGG ANCANNCCNN CTCCANCCNG TNNCTGCTGA TGTGGTCANC GNCNNNGGGG 961 NANCATNCAT CCTNCANCNN NNNNCNGNNN NNNNNTCAGC GNCNAANCNN NNNNNNNN //
Primer: IRES-F Region: IRES2, BSD, WPRE Sequence file: RDB17499_A9Iof.seq
>D06551B1_A9Io_2_IRES-F_H01_22_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NCNAGGTNNN NNNNACGTCT AGGCCCCCCG AACCNCGGGG    61 ACGTGGTTTT CCTTTGAAAA ACACGATGAT AATATGGCCA CAACCATGGC CAAGCCTTTG   121 TCTCAAGAAG AATCCACCCT CATTGAAAGA GCAACGGCTA CAATCAACAG CATCCCCATC   181 TCTGAAGACT ACAGCGTCGC CAGCGCAGCT CTCTCTAGCG ACGGCCGCAT CTTCACTGGT   241 GTCAATGTAT ATCATTTTAC TGGGGGACCT TGTGCAGAAC TCGTGGTGCT GGGCACTGCT   301 GCTGCTGCGG CAGCTGGCAA CCTGACTTGT ATCGTCGCGA TCGGAAATGA GAACAGGGGC   361 ATCTTGAGCC CCTGCGGACG GTGCCGACAG GTGCTTCTCG ATCTGCATCC TGGGATCAAA   421 GCCATAGTGA AGGACAGTGA TGGACAGCCG ACGGCAGTTG GGATTCGTGA ATTGCTGCCC   481 TCTGGTTATG TGTGGGAGGG CTAAGTCGAG CCTCTAGAGT TAACATCGAG GGATCAAGCT   541 TATCGATAAT CAACCTCTGG ATTACAAAAT TTGTGAAAGA TTGACTGGTA TTCTTAACTA   601 TGTTGCTCCT TTTACGCTAT GTGGATACGC TGCTTTAATG CCTTTGTATC ATGCTATTGC   661 TTCCCGTATG GCTTTCATTT TCTCCTCCTT GTATAAATCC TGGTTGCTGT CTCTTTATGA   721 GGAGTTGTGG CCCGTTGTCA GGCAACGTGG CGTGGTGTGC ACTGTGTTTG CTGACGCAAC   781 CCCCACTGGT TGGGGCATTG CCACCACCTG TCAGCTCCTT TCCGGGANTT TCGCTTTCCC   841 CCTCCCNATT GCCACGGCGG AACTCATCGC CGCCTGCCTT GCCCGCTGCT GGANAGGGGC   901 TCGGCTGTTG GGCACTGACN ATTNCGTGGT GTTGNCGGGG AANCTGANGT CCTTTNCNTG 961 GCTGCNNGCC TGNGTTGCNN NNNNNNNN //
Primer: BGH_rev2 Region: bGH p(A), 3' LTR, WPRE Sequence file: RDB17499_A9Iog.seq
>D06551B1_A9Io_2_BGH_rev2_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNG GGCAAACNAC AGATGGCTGG CAACTAGAAG    61 GCACAGTCGA GGCTGATCAG CGGGTTTAAA CGGGCCCTGC TAGAGATTTT CCACACTGAC   121 TAAAAGGGTC TGAGGGATCT CTAGTTACCA GAGTCACACA ACAGACGGGC ACACACTACT   181 TGAAGCACTC AAGGCAAGCT TTATTGAGGC TTAAGCAGTG GGTTCCCTAG TTAGCCAGAG   241 AGCTCCCAGG CTCAGATCTG GTCTAACCAG AGAGACCCAG TACAGTCCGG ATGCAGCTCT   301 CGGGCCATGT GATGAAATGC TAGGCGGCTG TCAAACCTCC ACTCTAATAC TTCTCTCTCC   361 GGGTCATCCA TCCCATGCAG GCTCACAGGG TGTAACAAGC GGGTGTTCTC TCCTTCATTG   421 GCTTCTTCTA CCTTCTCTTG CTCAACTGGT ACTAGCTTGT AGCACCATCC AAAGGTCAGT   481 GGATATCTGA TCCCTGGCCC TGGTGTGTAG TTCTGCCAAT CAGGGAAGTA GCCTTGTGTG   541 TGGTAGATCC ACAGATCAAG GATATCTTGT CTTCGTTGGG AGTGAATTAG CCCTTCCAGT   601 CCCCCCTTTT CTTTTAAAAA GTGGCTAAGA TCTACAGCTG CCTTGTAAGT CATTGGTCTT   661 AAAGGTACCT GAGGTGTGAC TGGAAAACCC ACCTCCTCCT CCTCTTGTGC TTCTAGCCAG   721 GCACAATCAG CATTGGTAGC TGCTGTATTG CTACTTGTGA TTGCTCCATG TTTTTCTAGG   781 TCTCGACGGT ATCGATGCGG GGAGGCGGCC CAAAGGGAGA TCCGACTCGT CTGANGGCGA   841 ANGCGAAGAN GCGGAAGANG CCGCANANNN GGCAGCANGC CGCGGGAANG AAGGTCCGCT   901 GNNNTGANGG CCGNANGGNN NGTANCNNAA NGNCGNNCCG CGCAGAATCC NNGNTGGNNN   961 CACANGNNGA NCAGCCNTGG AANNNNNNNC AGNNNNCCCG ANNNCACCAC NGNANNNNNN 1021 NNNN //
Primer: SV40pro_ori_F Region: SV40 pro_ori, EM7 pro, BleoR, SV40 p(A) Sequence file: RDB17499_A9Ioh.seq
>D06551B1_A9Io_2_SV40pro_ori_F_A02_02_ABI24.ab1     1 NNNNNNNNNN NNNNNNNGCC NCTGCCTCTG AGCTNTTCNN GAAGTAGTGA GGAGGCTTTT    61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC CGGGAGCTTG TATATCCATT TTCGGATCTG   121 ATCAGCACGT GTTGACAATT AATCATCGGC ATAGTATATC GGCATAGTAT AATACGACAA   181 GGTGAGGAAC TAAACCATGG CCAAGTTGAC CAGTGCCGTT CCGGTGCTCA CCGCGCGCGA   241 CGTCGCCGGA GCGGTCGAGT TCTGGACCGA CCGGCTCGGG TTCTCCCGGG ACTTCGTGGA   301 GGACGACTTC GCCGGTGTGG TCCGGGACGA CGTGACCCTG TTCATCAGCG CGGTCCAGGA   361 CCAGGTGGTG CCGGACAACA CCCTGGCCTG GGTGTGGGTG CGCGGCCTGG ACGAGCTGTA   421 CGCCGAGTGG TCGGAGGTCG TGTCCACGAA CTTCCGGGAC GCCTCCGGGC CGGCCATGAC   481 CGAGATCGGC GAGCAGCCGT GGGGGCGGGA GTTCGCCCTG CGCGACCCGG CCGGCAACTG   541 CGTGCACTTC GTGGCCGAGG AGCAGGACTG ACACGTGCTA CGAGATTTCG ATTCCACCGC   601 CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCGGCT GGATGATCCT   661 CCAGCGCGGG GATCTCATGC TGGAGTTCTT CGCCCACCCC AACTTGTTTA TTGCAGCTTA   721 TAATGGTTAC AAATAAAGCA ATAGCATCAC AAATTTCACA AATAAAGCAT TTTTTTCACT   781 GCATTCTAGT TGTGGTTTGT CCAAACTCAT CAATGTATCT TATCATGTCT GTATACCGTC   841 NANCTCTANC TAGAGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT GAAATTGTTA   901 TCCGCTCACA ATTNNNCACA ACATACGANC CGGAANCATA AAGTGTAAAG CCNGGGGGNG   961 NCTNANGANT GANCTAACTC NCATNNNTNG CGTTNCNNNN NNNNNCNNNN TTNNNNNNNG 1021 GNNANNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

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