Please review the QC test results indicated by icon below as well as clone information before placing your order.

pENTR hRFWD3

Gateway(R) Entry vector of human ring finger and WD repeat domain 3 (RFWD3).

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.  Order form [Credit Card Payment]  [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
Remarks	Gateway® Entry clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Inano, S et al. Mol. Cell, 66(5): 622-634, 2017)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
備考	Gateway®テクノロジーを用いたエントリークローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17502_A9Iop1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: rrnBT1ter_F2 Region: rrnB T1 ter, attL1, insert 5' Sequence file: RDB17502_A9Ioa.seq
>D06551B4_A9Io_2_rrnBT1ter_F2_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCCTTTCGT TTNNTTTGAT GCCTGGCAGT TCCCTACTCT    61 CGCGTTAACG CTAGCATGGA TGTTTTCCCA GTCACGACGT TGTAAAACGA CGGCCAGTCT   121 TAAGCTCGGG CCCCAAATAA TGATTTTATT TTGACTGATA GTGACCTGTT CGTTGCAACA   181 AATTGATGAG CAATGCTTTT TTATAATGCC AACTTTGTAC AAAAAAGCAG GCTCCGCGGC   241 CGCCCCCTTC ACCATGGCTC ATGAAGCAAT GGAATATGAT GTTCAGGTGC AGTTAAATCA   301 TGCCGAACAA CAGCCAGCTC CTGCTGGCAT GGCCAGCAGC CAAGGGGGAC CAGCCCTCCT   361 CCAGCCTGTT CCTGCTGATG TGGTCAGCAG CCAGGGGGTA CCATCCATCC TCCAGCCAGC   421 TCCTGCTGAG GTGATCAGCA GCCAAGCGAC ACCACCCCTG CTCCAGCCTG CTCCGCAACT   481 GTCTGTTGAC CTGACAGAAG TGGAGGTCTT GGGAGAAGAC ACTGTGGAGA ACATCAATCC   541 AAGAACTTCA GAACAACATA GGCAGGGATC TGATGGTAAT CACACCATCC CAGCATCTTC   601 GTTGCATTCA ATGACCAACT TCATCAGCGG ACTGCAGAGA CTTCATGGCA TGCTGGAATT   661 CCTGAGACCT TCATCTTCAA ACCACAGTGT AGGGCCAATG AGAACAAGAA GGAGGGTATC   721 TGCTTCACGG AGGGCAAGAG CCGGANGGTC TCAGANGACA GACAGTGCCA GGTTGAGAGC   781 ACCATTGGAT GCTTACTTTC AGGTGAGCAG GANCCAGCCT GACTTGCCAG CTACCACTTA   841 TGATTCAGAN ACTANGANTC CTGTATCTGA AGANTTGCAN GTGTCTAGTA NTTCTGATTC   901 TGACAGTGAC AGCTCTGCAG ANTATGGANG GGGNTGTTGA NNNNNCANAN GNANNTGGAN 961 CTNNCATTTT NANAANNANC NACNANNNNN NNGTCTCANC ANNNNNNNNN N //
Primer: KanR-N_R Region: attL2, insert 3' Sequence file: RDB17502_A9Iob.seq
>D06551B4_A9Io_2_KanR-N_R_B01_02_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNAGCATC NNTGTTGNNN NNTAATCGCG GCCTCGACGT    61 TTCCCGTTGA NNNNNNNNNN NNNNACCCCT TGTATTACTG TTTATGTAAG CAGACAGTTT   121 TATTGTTCAT GATGATATAT TTTTATCTTG TGCAATGTAA CATCAGAGAT TTTGAGACAC   181 GGGCCAGAGC TGCCAGGAAA CAGCTATGAC CATGTAATAC GACTCACTAT AGGGGATATC   241 AGCTGGATGG CAAATAATGA TTTTATTTTG ACTGATAGTG ACCTGTTCGT TGCAACAAAT   301 TGATAAGCAA TGCTTTCTTA TAATGCCAAC TTTGTACAAG AAAGCTGGGT CGGCGCGCCC   361 ACCCTTCTCC CACTTATAGA TGTGGACCAT CTTCTCTGTT AAGGTAGCCA AGTAGCTGTT   421 ACGGTTCACC TCAAATGGGC AGATGTCCAA CACAGGCTGA TCGGTCTGTA GGTCCTGGAG   481 CAACGAGCCA CTGGCAGCAT CCCACAGCAG GGCAGAATTT GCTGCTTCAT CCCCAGTNNN   541 NNNCAGGATG TTGCCATCAT TCTCTGGGCT TTGGAAAATG GCATTTTTGG NCNATAGTTT   601 GCNAGTAGGT CCTCCAAAAA ATGTATGTAC AGGCTGGCAN GAGCAGATTG NANTTCCAGN   661 GTCNTCCAGT CNGTAGGACA TTTNCATCAG NNCACTTCGN ATNGNNGNNG TGNNTTTTNN 721 CNNNNNNNNN N //
Primer: hRFWD3-N_F Region: insert mid Sequence file: RDB17502_A9Ioc.seq
>D06551B4_A9Io_2_hRFWD3-N_F_C01_03_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTGANN ANNTNCAGGT GTCTAGTAGT TCTGANTCTG    61 ACANNNNNNN NCTCTGCAGA GTATGGAGGG GTTGTTGACC AGGCAGANGA ATCTGGAGCT   121 GTCATTTTAG AAGAGCAACT AGCAGGTGTC TCAGCAGAGC AAGAAGTTAC ATGTATCGAT   181 GGAGGCAAGA CCCTCCCCAA ACAGCCATCT CCCCAGAAGT CTGAGCCTCT GCTACCTTCT   241 GCTTCTATGG ATGAGGAAGA AGGGGACACT TGTACAATAT GTCTGGAACA GTGGACCAAT   301 GCTGGGGACC ACCGGCTCTC AGCATTACGC TGTGGGCATC TCTTTGGGTA TAGGTGCATT   361 TCCACGTGGC TTAAAGGACA AGTACGAAAA TGTCCCCAGT GCAACAAGAA AGCCAGGCAC   421 AGTGACATTG TCGTCCTTTA TGCCCGAACC CTGAGAGCTT TGGACACTAG TGAACAGGAG   481 CGCATGAAAA GTTCCCTACT GAAGGAACAG ATGCTAAGGA AACAGGCCGA GTTAGAATCA   541 GCACAGTGCC GACTCCAACT GCAGGTCCTC ACTGATAAGT GCACTAAGCT TCAAAGGCGT   601 GTTCAGGACT TGNAAAAACT TACGTCACAT CAAAGTCAGA ATTTACAGCA ACCCAGGGGC   661 TCCCAAGCAT GGGTCCTGAG CTGCTCACCC TCCAGCCAGG GCCAGCACAA GCNCAAGTAC   721 CACTTCCAAA AGANCTTCAC AGTATCTCAG GCAGGAAACT GCCGGATCAT GGCATACTGT   781 GATGCTCTGA GCTGCCTGGT GATATCACAG CCNNCTCCTC NNNNCTCTTT TCTNCCANGC   841 TTTGGTGTTN AGATGTTGAG TACTGCCNAC ATGANNAGCA GTCAGTACAT TNCGATGCNT   901 GGGCAANNAG ATNCGNNGNA CNNNNNTTTA NCNNTNACNT CNNANGCTTG CNANTNNCTG 961 NTNNNNNANN NNNCANNATN AAACNNNNNN NNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2022.05.17

GNP_filter3_RDBDEP_html_220516.pl