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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pET28-3C-eIF2Ba (#RDB17722)

Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit alpha (eIF2Balpha).

Alternative name eIF2Balpha
Clone info. Bacterial expression vector of human eukaryotic translation initiation factor 2B subunit alpha (eIF2Balpha) cloned into the pET-28c, in which the thrombin cleavage site was replaced by the HRV 3C protease cleavage site.
Comment Expression was confirmed by the depositor.
Vector backbone pET28c (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Kanamycin
Gene/insert name human EIF2B1 cDNA
Depositor|Developer Ito, Takuhiro |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17722 pET28-3C-eIF2Ba DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET28-3C-eIF2Ba was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17722).

Reference section:

Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17722_B0Adp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro_R1 (Pr0762)
Region: lacO, RBS, 6xHis, HRV 3C site, insert 5'
Sequence file: RDB17722_B0Ada.seq check
>D06721A1_B0Ad_3_lacIpro_R1_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNCNAN NNNGCGCTCA TGANCCCGAA GTGGCGAGCC
   61 CGATCTTCCC CATCGGTGAT GTCNGCGATA TAGGCGCCAG CAACCGCACC TGTGGCGCCN
  121 GNGNNGNCGG CCACGATGCG TCCGGCGTAG AGGATCGAGA TCTCGATCCC GCGAAATTAA
  181 TACGACTCAC TATAGGGGAN TTGNGAGCGG ATAACAATTC CCCTCTAGAA ATAATTTTGT
  241 TTAACTTTAA GAAGGAGATA TACCATGGGC AGCAGCCATC ATCATCATCA TCACAGCAGC
  301 GGCCTTGAAG TCCTCTTTCA GGGACCCGGG CATATGGACG ACAAGGAGTT AATTGAATAC
  361 TTTAAGTCTC AGATGAAAGA AGATCCTGAC ATGGCCTCAG CAGTGGCTGC CATCCGGACG
  421 TTGCTGGAGT TCTTGAAGAG AGATAAAGGG GAGACAATCC AGGGTCTGAG GGCGAATCTC
  481 ACCAGTGCCA TAGAAACCCT GTGTGGTGTG GACTCCTCTG TGGCAGTGTC CTCTGGCGGG
  541 GAGCTCTTCC TCCGCTTCAT CAGTCTTGCC TCCCTGGAAT ACTCCGATTA CTCCAAATGT
  601 AAAAAGATCA TGATTGAGCG GGGAGAACTT TTTCTCAGGA GAATATCACT GTCAAGAAAC
  661 AAAATTGCAG ATCTGTGCCA TACTTTCATC AAAGATGGAG CGACAATATT GACTCACGCC
  721 TACTCCAGAG TGGTCCTGAN AGTCCTGGAA NNCAGCNNNN NCGGNCAAGA NGCGATTTAG
  781 TGTATACGTC ACAGAGTCAC ANCCTGATTT GTCAGGNAAG AAAATGGCCA AAGCCNNCNN
  841 NCACCTCAAC GTNCCTGNCA CTGTGNTGCT AGATGCTGCT GTCNGGCTAC NTNNNNGAGA
  901 AAGCAGATCT TGNCATANNT NNGTGCTGAN NGAGTTGTTN AAAACNGNNN GNATTNNTNA
961 CANNNNNNGA NNNNNNNNNN
//
Primer: T7 terminator (Pr0155)
Region: insert 3'
Sequence file: RDB17722_B0Adb.seq check
>D06721A1_B0Ad_3_T7 terminator_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGNNGNTTN NNNNNCNNNC CGGNTCTCNG TGGNNNGTGG
   61 TGGTGGTGCT CGAGTGGGCC GCTCACAGAT AGAGCTTGAT GAGCTCATCG CTGACTGCTG
  121 AGGGTGTCAG CACGCCCAGG TCTGTAAACA GCAGAGTGAT TAAGGAAGGG GCAGTGTAGT
  181 CGACCCACGG ATGCTCCTCT TTGAGGTCTT GTCCAGTCTG CGCGACCTTG AGAGTGTCTG
  241 CCTTATACTT AAACTTATCT GGGACGTCTT GCTGGTTTAG TGGAAAGAGC CGGACAAACT
  301 TGAAACTTTC TGCAACCACA TAGAAAGGTT TGTTCTGTGC TTTGGCACAC ACAGCCATCT
  361 GGTTGGTTCC AATCTTGTTA ATAATTCCTC CGTTTTCAAC AACTCCTTCA GCACCAACTA
  421 TGACAAGATC TGCTTTCTCC ATGATGTAGC CGACAGCAGC ATCTAGCACC ACAGTGACAG
  481 GGACGTTGAG GTGGCAGAGG GCTTTGGCCA TTTTCTTACC TGACAAATCA GGCTGTGACT
  541 CTGTGACGTA TACACTAAAT CGCTTCTTGG CCGCCACGGC TGCTTCCAGG ACTCTCAGGA
  601 CCACTCTGGA GTAGGCGTGA GTCAATATTG TCGCTCCATC TTTGATGAAA GTATGGCACA
  661 GATCTGCAAT TTTGTTTCTT GACAGTGATA TTCTCCTGAG AAAAAGTTCT CCCCGCTCAA
  721 TCATGATCTT TTTACATTTG GAGTNANNCG GANTATTCCA GGGAGGCAAG ANTGATGAAG
  781 CGGANGAAGA NCTCCCCGCC AGNNGACACT GCCACAGANG ANTCCACACC ACACAGGGTT
  841 TCTATGGCAC TGGTGANANT NNNNCCNCAN ANCCNGNNNT GTCTCCCCTT NATCTCNCTT
  901 CNAGANNTNC NNNNNNNNTC CNGNTGGCAG CCNCNGNNTN ANNNNNTGTC NGGATCTTCT
  961 TTCNTCTGAN ANTTAAANNN ANTNCNANTN NNTNCNTNNN NNGNNCNNTA TNNCCNNGNN
1021 NNCCNNNNNN NNNGNNNNTN NNNNNNNNN
//
Primer: pET_lacIpro_F2 (Pr0646)
Region: lacI pro, lacI
Sequence file: RDB17722_B0Adc.seq check
>D06721A1_B0Ad_3_pET_lacIpro_F2_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCNNTNG CTGGCGCCTA TATCGCCGAC ATCACCGATG
   61 GGGAAGATCG GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG
  121 CAGGCCCCGT GGCCGGGGGA NTGTTGGGCG CCATCTCCTT GCATGCACCA TTCCTTGCGG
  181 CGGCGGTGCT CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA
  241 AGGGAGAGCG TCGAGATCCC GGACACCATC GAATGGCGCA AAACCTTTCG CGGTATGGCA
  301 TGATAGCGCC CGGAAGAGAG TCAATTCAGG GTGGTGAATG TGAAACCAGT AACGTTATAC
  361 GATGTCGCAG AGTATGCCGG TGTCTCTTAT CAGACCGTTT CCCGCGTGGT GAACCAGGCC
  421 AGCCACGTTT CTGCGAAAAC GCGGGAAAAA GTGGAAGCGG CGATGGCGGA GCTGAATTAC
  481 ATTCCCAACC GCGTGGCACA ACAACTGGCG GGCAAACAGT CGTTGCTGAT TGGCGTTGCC
  541 ACCTCCAGTC TGGCCCTGCA CGCGCCGTCG CAAATTGTCG CGGCGATTAA ATCTCGCGCC
  601 GATCAACTGG GTGCCAGCGT GGTGGTGTCG ATGGTAGAAC GAANNGGCGT CGAAGCCTGT
  661 AAAGCGGCGG TGCACAATCT TCTCGCGCAA CGCGTCAGTG GGCTGATCAT TAACTATCCG
  721 CTGGATGACC AGGATGCCAT TGCTGTGGAA GCTGCCTGCA CTAATGTTCC GGCGTTATTT
  781 CTTGATGTCT CTGACCAGAC ACNCATCNAC AGTATTATTT TCTCCCNTNA AGACNGTACG
  841 CGACTGGGCG TGGNNCATCT GGTCGCATNN GGGTCACCAG CAAAATCGCG CTGTTNGCGG
  901 GCCCNNTAAG TTCTGNNTNN GNNGCGTCTG CNNCNNGNNT GNCTNGCATN AANNNNNNTC
961 NNTCNNNNNC NAANTNNNGN CNATANCNGN NANNGGNNAN NNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]