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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEBMulti-Neo-human-eIF2gamma-FlagHis8 (#RDB17727)

Expression vector of human eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma).

Alternative name eIF2gamma
Clone info. Expression vector of human eukaryotic translation initiation factor 2 subunit gamma (eIF2gamma) cloned into the pEBMulti-Neo ,tagged with FLAG and His8 at C-terminus.
Comment Expression was confirmed by the depositor.
Vector backbone pEBMulti-Neo (plasmid)
Size of vector backbone 10.0 kb
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cells).
Gene/insert name human EIF2S3 cDNA
Depositor|Developer Ito, Takuhiro |
 
Sequence (partial) RDB17727pka02.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kashiwagi, K. et al. Science, 364(6439), 495-499, 2019)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB17727 pEBMulti-Neo-human-eIF2gamma-FlagHis8 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEBMulti-Neo-human-eIF2gamma-FlagHis8 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB17727).

Reference section:

Kashiwagi, K., Yokoyama, T., Nishimoto, M., Takahashi, M., Sakamoto, A., Yonemochi, M., Shirouzu, M., Ito, T., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17727_A9Kcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CAG_R1 (Pr0584)
Region: CAC pro, insert 5'
Sequence file: RDB17727_A9Kca.seq check
>D06721A6_B0Ju_1_CAG_R1_B09_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNC GGGCGCGGGG CGNANCGGTG CGGCGCCGGC
   61 AGGAAGGAAA TGGGCGGNNN NGNCCTTCGT GCGTCGCCGC GCCGCCGTCC CCTTCTCCCT
  121 CTCCAGCCTC GGGGCTGTCC GCGGGGGGAC GGCTGCCTTC GGGGGGGACG GGGCAGGGCG
  181 GGGTTCGGCT TCTGGCGTGT GACCGGCGGC TCTAGAGCCT CTGCTAACCA TGTTCATGCC
  241 TTCTTCTTTT TCCTACAGCT CCTGGGCAAC GTGCTGGTTA TTGTGCTGTC TCATCATTTT
  301 GGCAAAGAAT TAACCCTCAC TAAAGGGGTA CCGGGCCCCC CCTCGAGGTC GACGCCACCA
  361 TGGCGGGCGG AGAAGCTGGA GTGACTCTAG GGCAGCCGCA TCTTTCGCGT CAGGATCTCA
  421 CCACCTTGGA TGTTACCAAG TTGACGCCAC TTTCACATGA AGTTATCAGC AGACAAGCCA
  481 CAATTAACAT AGGTACAATT GGTCATGTAG CTCATGGGAA ATCCACAGTC GTCAAAGCTA
  541 TTTCTGGAGT TCATACTGTC AGGTTCAAAA ATGAACTAGA AAGAAATATT ACAATCAAGC
  601 TTGGATATGC TAATGCTAAG ATTTATAAGC TTGATGACCC AAGTTGCCCT CGGCCAGAAT
  661 GTTATAGATC TTGTGGGAGC AGTACACCTG ACGAGTTTCC TACGGACATT CCAGGGACCA
  721 AAGGGAACTT CAAATTAGTC AGACATGTTT CCTTTGTTGA CTGTCCTGNC CACGATATTT
  781 TGATGGCTAC TATGCTGAAC GGTGCAGCAG TGATGGATGC AGCTCTTCTG TTGATAGCTG
  841 GTAATGAATC TTGCCCTCNN NCTCANANAT CGGAACACCT GGCTGCTATA GAGNTCATGA
  901 AACTGAANCA TATTTTGATT CTACAAAATA AAAANTGATT TGGNNAAANA AANNTCNNNN
961 NANNNANCNN NNNNANCANA NNNNTNGCAN TTNNNCCNNN NNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: insert 3' (8xHis, FLAG, hEIF2S3)
Sequence file: RDB17727_A9Kcb.seq check
>D06721A6_A9Kc_1_SV40polyA-R2_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NANNANGTTA ACNANNANNA TTGCATTCAT TTTATGTTTC
   61 AGGTTCAGGG GGAGGTGTGG GAGGTTTTTT AAAGCAAGTA AAACCTCTAC AAATGTGGTA
  121 TGGCTGATTA TGATCTAGAG TCGCGGCCGC TCAATGATGA TGATGATGAT GATGATGTTT
  181 ATCGTCATCG TCTTTGTAGT CGTCATCATC TACTGTTGGC TTGATTGTCA CTCCTCTTCT
  241 TATCTGACCC CAACCAATTA AACGCCAGTG TTTTTCAACT CTTCGGCTAA GGGCAATTTT
  301 TTCTCCTACC TCTGTGCACA CTGGATTGGT CAAAACAATT TTACCCAAAT CGGCCTTGAC
  361 AGCACTAACT CTCCCTCCTG TTGACAGGGA TCCTATGTTC ACCATGAGCA CTTCATTCTT
  421 AGACAGCTTT TGAACCTTTG CTGCTTTCTT GTCTCCTTCA GTGCGTACAC CTAGAAGCCG
  481 TCTAAGCAGG AAATAGGAAA TTTCCAATTC TGTGAATATC TCAGGTAAAG CTCCGACTGC
  541 ACCAAGTACT TGCCCCACCA TTCTGTCAGC CCGGCACAAA GTGGGGTCAA TTTTTGTTCC
  601 AACTCCAATA ANANCGCCTG GAGCAGCATA TTGCANATCA TTATGCTCCG CAAAAAGTGA
  661 TACAATTTTG GAAAAANATT GGTTTACNNC NTGAGTTTNC CTTCNNCTAT CTTTGNAAAC
  721 AATACCAGGN CTTACNTTCT ATCTCCTGNN CNANCTTTNA ATACTCCNNT TTANGANNNT
  781 NNCACNNNNT ACNNNTNCCC TNANNGNNNN NNANNTTCAC NNNNNNNGTT TGNTNNNCNT
  841 CAAANATCTA NTAACAATAA GNCNGGGGCT CTGAANNAAA NNNTCNTGNG NGTNNNNGNN
901 NNTTNNNTTT NCNNNGNNNN NNNNCNANNN NNNNNNN
//
Primer: oriP_F (Pr0765)
Region: oriP, EBNA-1
Sequence file: RDB17727_A9Kcc.seq check
>D06721A6_A9Kc_1_oriP_F_D02_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG GGGCGTGNAT TTTCGCTGCT TGTCCTTTTC
   61 CTGCTGGTTG CTCCCATTCT TAGGTGAATT TAAGGAGGCC AGGCTAAAGC CGTCGCATGT
  121 CTGATTGCTC ACCAGGTAAA TGTCGCTAAT GTTTTCCAAC GCGAGAAGGT GTTGAGCGCG
  181 GAGCTGAGTG ACGTGACAAC ATGGGTATGC CCAATTGCCC CATGTTGGGA GGACGAAAAT
  241 GGTGACAAGA CAGATGGCCA GAAATACACC AACAGCACGC ATGATGTCTA CTGGGGATTT
  301 ATTCTTTAGT GCGGGGGAAT ACACGGCTTT TAATACGATT GAGGGCGTCT CCTAACAAGT
  361 TACATCACTC CTGCCCTTCC TCACCCTCAT CTCCATCACC TCCTTCATCT CCGTCATCTC
  421 CGTCATCACC CTCCGCGGCA GCCCCTTCCA CCATAGGTGG AAACCAGGGA GGCAAATCTA
  481 CTCCATCGTC AAAGCTGCAC ACAGTCACCC TGATATTGCA GGTAGGAGCG GGCTTTGTCA
  541 TAACAAGGTC CTTAATCGCA TCCTTCAAAA CCTCAGCAAA TATATGAGTT TGTAAAAAGA
  601 CCATGAAATA ACAGACAATG GACTCCCTTA GCGGGCCAGG TTGTGGGCCG GGTCCAGGGG
  661 CCATTCCAAA GGGGAGACGA CTCAATGGTG TAAGACGACA TTGTGGAATA NCAAGGGCAG
  721 TTCCTCGCCT TANGTTGTAA AGGGAGGTCT TANTACCTCC ATATACGANC ACACCGGCGA
  781 NCCAAGTTCC TTCGTCNGTA NTCCTTTCTA CGTGACTCCT AGCCAGGANA GCTCTTAAAC
  841 CTTCTGCAAT GNTCTCAAAT TTCNGNNTGG ANNCTNCTTG NNCACGATGC TTTCNNAACC
  901 ANCCTCCTTT TTTGCNCCTG NNTNCATCAC CCTGANCCCG GGGNCNAGNG NNTGGGNCTT
961 CNNCNNNNNC ATCNGCGGGN CCNNNNNCTA TCGCTNCNNG GGGGCNCNNN NNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40 pro_ori, NeoR_KanR
Sequence file: RDB17727_A9Kcd.seq check
>D06721A6_A9Kc_1_SV40pro_ori_F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNANN NNNNNCTCGN CCTCTGANCT ATTCNNGAAG TAGTGAGGAG
   61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAG ATCATCAAGA GACAGGATGA GGATCGTTTC
  121 GCATGATTGA ACAAGATGGA TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT
  181 TCGGCTATGA CTGGGCACAA CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT
  241 CAGCGCAGGG GCGCCCGGTT CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC
  301 TGCAAGACGA GGCAGCGCGG CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG
  361 TGCTCGACGT TGTCACTGAA GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC
  421 AGGATCTCCT GTCATCTCAC CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA
  481 TGCGGCGGCT GCATACGCTT GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC
  541 GCATCGAGCG AGCACGTACT CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG
  601 AAGAGCATCA GGGGCTCGCG CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG AGCATGCCCG
  661 ACGGCGANGA TCTCGTCGTG ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA
  721 ATGGCCGCTT TTCTGGANTC NTCNNCTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCANG
  781 ANATANCGTT GGCTANCCGT GATATTGCTG AANAGCTTGN CGGCNAATGG GNCTGANCGC
  841 TNCCNNGNGN TTNCNGTATC GNCGCTCCCG NNTTCNNAGC NGCNTCNNCN TTNNNATCGC
  901 CTTCNTGANN NNNNNNTTCT GANCGGGNNN NNTNGGGGTT CGAANNNNNN NNANNNNNCN
  961 ACGCCCNNNC NNGNNNNCNC NNNNANTTNC NNNTNCNNNC NCNNNCNTNN NNNNAANNNN
1021 NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kashiwagi, K., Structural basis for eIF2B inhibition in integrated stress response. Science 364 (6439): 495-499 (2019). PMID 31048492. [link to RRC of NBRP]