Please review the QC test results indicated by icon below as well as clone information before placing your order.

pUC18Z_ePAG2

Cloning vector harboring counterselection marker Escherichia coli phenylalanyl-tRNA synthetase a-subunit (ePheS).

Sequence (full)	RDB17948zzk01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyazaki K. et al. Biotechniques, 58 (2): 86-88, 2015)。
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

References and tips

Original reference

Further references such as user reports and related articles (go to bottom)

Featured content

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB17948_B0Bcp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ZeoR-C_F (Pr0829) Region: insert mid (ZeoR 3', pheS 5') Sequence file: RDB17948_B0Bca.seq
>D06895A6_B0Bc_2_ZeoR-C_F_D03_12_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NGNNNNNNGC CGNNNNNNGC GTGCNCTTCG TGGCCGANGA    61 NCAGGACTAA AGGAGGTAAA ACACATGTCA CATCTCGCAG AACTGGTTGC CAGTGCGAAG   121 GCGGCCATTA GCCAGGCGTC AGATGTTGCC GCGTTAGATA ATGTGCGCGT CGAATATTTG   181 GGTAAAAAAG GGCACTTAAC CCTTCAGATG ACGACCCTGC GTGAGCTGCC GCCAGAAGAG   241 CGTCCGGCAG CTGGTGCGGT TATCAACGAA GCGAAAGAGC AGGTTCAGCA GGCGCTGAAT   301 GCGCGTAAAG CGGAACTGGA AAGCGCTGCA CTGAATGCGC GTCTGGCGGC GGAAACGATT   361 GATGTCTCTC TGCCAGGTCG TCGCATTGAA AACGGCGGTC TGCATCCGGT TACCCGTACC   421 ATCGACCGTA TCGAAAGTTT CTTCGGTGAG CTTGGCTTTA CCGTGGCAAC CGGGCCGGAA   481 ATCGAAGACG ATTATCATAA CTTCGATGCT CTGAACATTC CTGGTCACCA CCCGGCGCGC   541 GCTGACCACG ACACTTTCTG GTTTGACACT ACCCGCCTGC TGCGTACCCA GACCTCTGGC   601 GTACAGATCC GCACCATGAA AGCCCAGCAG CCACCGATTC GTATCATCGC GCCTGGCCGT   661 GTTTATCGTA ACGACTACGA CCAGACTCAC ACGCCGATGT TCCATCAGAT GGAAGGTCTG   721 ATTGTTGATA CCNACATCAG CTTTACCAAC CTGAAAGGNA CGCTGCACGA CTTCCTGCGT   781 AACTTCTTTG ANGAAGANTT GCAGANTCGC TTCCGTCCTT CCTACTTCNC GTTTGCTGAN   841 CCTTCTGCAG AAGTGGANGT CNTGGGGTAA AAACGGTAAA TGGCTGGAAN TGCTGGGCTG   901 CGGGNNGGNG NATNCNNAAC GNGTTGCGNN NCGTNNNCNT CNANCCNGNA NNTTTACTCT   961 GGNTTCGGNT NCNGGANNGG GANGGANCGN CNGANTATGN NGCGNNNNNG CGNNNNNNAN  1021 NTGNGNNCNT TCNNNNNAAA CNNNNNNNNN TNNNNNNNNA GNTNNANNNN NNNNCNNGNN 1081 NNNNNNNN //
Primer: Zeo-M_R (Pr0675) Region: insert 5' (ZeoR 5') Sequence file: RDB17948_B0Bcb.seq
>D06895A6_B0Bc_2_Zeo-M_R_F03_18_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGCGNNNCC NCNCCCNGGC NNGGGTGTTG TCCGGCACCA    61 CCTGGTCCTG GACCGCGCTG ATGAACAGGG TCACGTCGTC CCGGACCACA CCGGCGAAGT   121 CGTCCTCCAC GAAGTCCCGA GAGAACCCGA GCCGGTCGGT CCAGAACTCG ACCGCTCCGG   181 CGACGTCGCG CGCGGTGAGC ACCGGAACGG CACTGGTCAA CTTGGCCATA CTCTTCCTTT   241 TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT   301 GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG   361 ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT ATCACGAGGC   421 CCTTTCGTCT CGCGCGTTTC GGTGATGACG GTGAAAACCT CTGACACATG CAGCTCCCGG   481 AGACGGTCAC AGCTTGTCTG TAAGCGGATG CCGGGAGCAG ACAAGCCCGT CAGGGCGCGT   541 CAGCGGGTGT TGGCGGGTGT CGGGGCTGGC TTAACTATGC GGCATCAGAG CAGATTGTAC   601 TGAGAGTGCA CCATGTGCGG TGTGAAATAC CGCACAGATG CGTAAGGAGA AAATACCGCA   661 TCAGGCGCCA TTCGCCATTC AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT   721 CTTCGCTATT ACGCCAGCTG GCGAAAGGGG GANGTGCTGC AAGGCGATTA AGTTGGGTAA   781 CGCCAGGGTT TTCCCAGTCA CGACGTTGTA AAACGACGGC CAGTGCCNAG CTTGCNTGNC   841 TGCANGNCGA NTCTAGAGGN NCCCCNGNTA NCGANCTCGA ATTCNTAATC NTGGNCNTAT   901 GTGTTTCCTG NGTGAAATTG NNATCCGCTC NNNATTCCNC NCNNNNTACN ANCCNNNNNN   961 NTAAAGNGNA AANNNTGGGN NGNNTNNNGA NNGANCTNNC TCNNNNTNNN NNGNNNNNNN 1021 NNN //
Primer: ePAG_R1 (Pr0828) Region: insert 3' (pheS 3') Sequence file: RDB17948_B0Bcc.seq
>D06895A6_B0Bc_2_ePAG_R1_E03_15_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NTNNCCTAGN TCCTTTTAAA TTAAAAATGA AGTTTTAAAT    61 CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGGAA TTATTATTTA AACTGTTTGA   121 GGAAACGCAG ATCGTTTTCG AAGAATGAAC GCAGGTCGGT GACGCCGTAA CGCAACATAG   181 TCAGACGCTC CATCCCCATC CCGAACCCGA AACCAGAGTA AACTTCCGGG TCGATGCCAA   241 CGTTACGCAA CACGTTCGGA TGCACCATCC CGCAGCCCAG CACTTCCAGC CATTTACCGT   301 TTTTACCCAT GACGTCCACT TCTGCAGAAG GTTCAGCAAA CGGGAAGTAG GAAGGACGGA   361 AGCGAATCTG CAAATCTTCC TCAAAGAAGT TACGCAGGAA GTCGTGCAGC GTGCCTTTCA   421 GGTTGGTAAA GCTGATGTTG GTATCAACAA TCAGACCTTC CATCTGATGG AACATCGGCG   481 TGTGAGTCTG GTCGTAGTCG TTACGATAAA CACGGCCAGG CGCGATGATA CGAATCGGTG   541 GCTGCTGGGC TTTCATGGTG CGGATCTGTA CGCCAGAGGT CTGGGTACGC AGCAGGCGGG   601 TAGTGTCAAA CCAGAAAGTG TCGTGGTCAG CGCGCGCCGG GTGGTGACCA GGAATGTTCA   661 GAGCATCGAA GTTATGATAA TCGTCTTCGA TTTCCGGCCC GGTTGCCACG GTAAAGCCNA   721 GCTCACCGAA GAAACTTTCG ATACGGTCGA TGGTACGGGT AACCGGANGC AGACCGCCGT   781 TTTCAATGCG ACGACCTGGC AGANAGACAT CNATCGTTTC CGCCGCCAGA NGCGCATTCA   841 GTGCAGCGCT TTCCAGTTCC GCTTTACGCG CATTCNGCGN CNGNTGAACC NGCTCTTTNG   901 CTNCNTTGAN AANCGCACCA GCTGCNNGAN GCTCNNCTGG NGGNNGCTCA CGNNNNNNGN 961 CATNNGNNNN NNNNAGNGNC NNTTTTTANC NNNNANTNNA NNNNCANANN NNNNNNNN //
Primer: SV40pA3'Element Reverse (Pr0108) Region: ColE1 ori, MCS Sequence file: RDB17948_B0Bcd.seq
>17948_17948_B0Bc_1_SV40pA3'elementReverse_H01_08_ABI08.ab1     1 NNNNNNNNNN GNNNNNNNNN NNNNNNNNNN NNNNNNCNNN NNNCGCGGCC TTTNNNNNNT    61 TCCTGGCCTT TTGCTGGCCT TTTGCTCACA TGTTCTTTCC TGCGTTATCC CCTGATTCTG   121 TGGATAACCG TATTACCGCC TTTGAGTGAG CTGATACCGC TCGCCGCAGC CGAACGACCG   181 AGCGCAGCGA GTCAGTGAGC GAGGAAGCGG AAGAGCGCCC AATACGCAAA CCGCCTCTCC   241 CCGCGCGTTG GCCGATTCAT TAATGCAGCT GGCACGACAG GTTTCCCGAC TGGAAAGCGG   301 GCAGTGAGCG CAACGCAATT AATGTGAGTT AGCTCACTCA TTAGGCACCC CAGGCTTTAC   361 ACTTTATGCT TCCGGCTCGT ATGTTGTGTG GAATTGTGAG CGGATAACAA TTTCACACAG   421 GAAACACATA TGACCATGAT TACGAATTCG AGCTCGGTAC CCGGGGATCC TCTAGAGTCG   481 ACCTGCAGGC ATGCAAGCTT GGCACTGGCC GTCGTTTTAC AACGTCGTGA CTGGGAAAAC   541 NNNNNNNNNA CCCAACTTAA TCGCCTTGCA NCACATCCCC CTTTCGCCAG CTGGCGNNAA   601 TANCGAAGAN GGNNNNNNCC NATCGCCCNT TCCNNNANAG NTNGCNCNNC CNGAATGGGN   661 NANNGGGCGC CTGATGCNNN NNTTTTTCNC CTTTNNNCNN CTGNGNNGNA TTNNANACNN 721 CNNNNNGNNG NNCNNNNNNN ANNNNCTGCN NNNNNANGNC NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.02.22

GNP_filter3_RDBDEP_html_230130.pl