RDB18238 - pGEX-G4Null

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pGEX-G4Null (#RDB18238)

Bacteria expression vector of human galectin-4 with a truncated linker peptide (G4Null), GST fusion protein

Clone info.	Bacterial expression clone of human galectin-4 with a truncated linker peptide.
Comment	Expression was confirmded by the depositor by affinity purification and SDS-PAGE.
Vector backbone	pGEX-4T-2 (Plasmid)
Selectable markers	Amp^r
Gene/insert name	Human galectin-4 cDNA
Depositor\|Developer	Nishi, Nozomu \|

Sequence (partial)	RDB18238pka01.seq provided by the depositor

Distribution information

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの利用は、学術機関の学術研究に限る。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18238	pGEX-G4Null	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pGEX-G4Null was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18238).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18238_B0Gfp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pGEX-F2 (Pr0403) Region: GST, thrombin site, Insert 5' Sequence file: RDB18238_B0Gfa.seq
>D07204A3_B0Gf_2_pGEX-F2_B02_05_ABI24.ab1 1 NNNNNNNNNN NNNTTNNANN NNGTATTGNN GCTATCCCNC AAATTGATAA GTACTTGAAA 61 TCCAGCAAGT ATATAGCATG GCCTTTGCAG GGCTGGCAAG CCACGTTTGG TGGTGGCGAC 121 CATCCTCCAA AATCGGATCT GGTTCCGCGT GGATCCCCAG GAATTCCCAT GGCCTATGTC 181 CCCGCACCGG GCTACCAGCC CACCTACAAC CCGACGCTGC CTTACTACCA GCCCATCCCG 241 GGCGGGCTCA ACGTGGGAAT GTCTGTTTAC ATCCAAGGAG TGGCCAGCGA GCACATGAAG 301 CGGTTCTTCG TGAACTTTGT GGTTGGGCAG GATCCGGGCT CAGACGTCGC CTTCCACTTC 361 AATCCGCGGT TTGACGGCTG GGACAAGGTG GTCTTCAACA CGTTGCAGGG CGGGAAGTGG 421 GGCAGCGAGG AGAGGAAGAG GAGCATGCCC TTCAAAAAGG GTGCCGCCTT TGAGCTGGTC 481 TTCATAGTCC TGGCTGAGCA CTACAAGGTG GTGGTAAATG GAAATCCCTT CTATGAGTAC 541 GGGCACCGGC TTCCCCTACA GATGGTCACC CACCTGCAAG TGGATGGGGA TCTGCAACTT 601 CAATCAATCA ACTTCATCGG AGGCCAGCCC CTCACCTTCA ACCCGCCTGT GCCATATTTC 661 GGGAGGCTGC AAGGAGGGCT CACAGCTCGA AGAACCATCA TCATCAAGGG CTATGTGCCT 721 CCCACAGGCA AGAGCTTTGC TATCAACTTC NAGGTGGGCT CCTCANGGGA NATAGCTCTG 781 CACATTAATC CCCGCATGGG CAACGGTACC GNGGTCCGGA ACAGCCTTCT GAATGGCTCG 841 TGGGGATNCG AGGANAANAA GATCACCCAC AACCCNTTTG GNNCCGGANA GTTCTTTGAT 901 CTGNNNNTTC NCTGNGGNTN GNATNGCTTC NAGGTTTACG CNNATGNNCN GCACCNCTTT 961 GANTTTGCCN NNNNNNNNTC NGNNNNNNNN NANGNNNNNN NNNN //
Primer: pJB8-Forward (Pr0084) Region: Insert 3' Sequence file: RDB18238_B0Gfb.seq
>D07204A3_B0Gf_2_pJB8-Forward_C02_08_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGNNGTNA TGGCTGCGCC CCGNCNCCCG CCAACACCCG 61 CTGACGCGCC CTGACGGGCT TGTCTGCTCC CGGCATCCGC TTACAGACAA GCTGTGACCG 121 TCTCCGGGAG CTGCATGTGT CAGAGGTTTT CACCGTCATC ACCGAAACGC GCGAGGCAGA 181 TCGTCAGTCA GTCACGATGC GGCCGCTCGA GTTAGATCTG GACATAGGAC AAGGTGACAT 241 CACCCTGGAT TTCCAATGTG TCCACCCTCT GGAAGGCCGA GAGGCGATGG GCAAAGTCAA 301 AGAGGTGCTG GCCATTGGCG TAAACCTTGA AGCGATCCAA GCCACAGCGA ATGGACAGAT 361 CAAAGAACTG TCCGGGACCA AATGGGTTGT GGGTGATCTT CTTCTCCTCG GATCCCCACG 421 AGCCATTCAG AAGGCTGTTC CGGACCACGG TACCGTTGCC CATGCGGGGA TTAATGTGCA 481 GAGCTATGTC CCCTGAGGAG CCCACCTTGA AGTTGATAGC AAAGCTCTTG CCTGTGGGAG 541 GCACATAGCC CTTGATGATG ATGGTTCTTC GAGCTGTGAG CCCTCCTTGC AGCCTCCCGA 601 AATATGGCAC AGGCGGGTTG AAGGTGAGGG GCTGGCCTCC GATGAAGTTG ATTGATTGAA 661 GTTGCAGATC CCCATCCACT TGCAGGTGGG TGACCATCTG TAGGGGAAGC CGGTGCCCGT 721 ACTCATAGAA GGGATTTCCA TTTACCACCA CCTTGTAGTG CTCAGCCAGG ACTATGAAGA 781 CCAGCTCAAA GGCGGCACCC TTTTTGAAGG GCATGCTCCT CTTCCTCTCC TCGCTGCCCC 841 ACTTCCCGCC CTGCAACGTG TTGAANANCA CCTTGNCCCA GCCGTNANNC GCGGANTGAA 901 GTGGAAGGCG ANGTCTGANC CNNNATCCTG NCNNACCNCA ANGTTCACGA ANAANNGCTT 961 CATGNNCTCG CTGGNCNNNN CNTNNNATNN NAACAGANNT TNCCNNNNNN ANCNNNCCNG 1021 GNNNNGGNNN NNNNNNN //
Primer: M13_-40 (Pr0410) Region: lacI Sequence file: RDB18238_B0Gfc.seq
>D07204A3_B0Gf_2_M13_-40_D02_11_ABI24.ab1 1 NNNNNNNNNN NNNNNNTNNT GGTCNTAGCT GTTTCCTGTG TGAAATTGTT ATCCGCTCAC 61 AATTCCACAC AACATACGAG CCGGAAGCAT AAAGTGTAAA GCCTGGGGTG CCTAATGAGT 121 GAGCTAACTT ACATTAATTG CGTTGCGCTC ACTGCCCGCT TTCCAGTCGG GAAACCTGTC 181 GTGCCAGCTG CATTAATGAA TCGGCCAACG CGCGGGGAGA GGCGGTTTGC GTATTGGGCG 241 CCAGGGTGGT TTTTCTTTTC ACCAGTGAGA CGGGCAACAG CTGATTGCCC TTCACCGCCT 301 GGCCCTGAGA GAGTTGCAGC AAGCGGTCCA CGCTGGTTTG CCCCAGCAGG CGAAAATCCT 361 GTTTGATGGT GGTTAACGGC GGGATATAAC ATGAGCTGTC TTCGGTATCG TCGTATCCCA 421 CTACCGAGAT ATCCGCACCA ACGCGCAGCC CGGACTCGGT AATGGCGCGC ATTGCGCCCA 481 GCGCCATCTG ATCGTTGGCA ACCAGCATCG CAGTGGGAAC GATGCCCTCA TTCAGCATTT 541 GCATGGTTTG TTGAAAACCG GACATGGCAC TCCAGTCGCC TTCCCGTTCC GCTATCGGCT 601 GAATTTGATT GCGAGTGAGA TATTTATGCC AGCCAGCCAG ACGCAGACGC GCCGAGACAG 661 AACTTAATGG GCCCGCTAAC AGCGCGATTT GCTGGTGANC CAATGCGACC AGATGCTCCA 721 CGCCCAGTCG CGTACCGTCT TCATGGGAGA AAATAATACT GTTGATGGGT GTCTGGTCAG 781 AGACATCAAG AAATAACGCC GGAACATTAG TGCAGGCAGC TTCCACAGCA ATGGNATCCT 841 GNTCATCCAG CGGATAGTTA ATGATCAGCC CACNGACGCG TNGCGCGANA ANANTGTGCA 901 NNGCCGCTTT ACAGGNTTCN ANGNCGCTTC NTNCNANCAT CGANNNCNNN NCGCTGGNAN 961 CNANNNGATN NGNNCNANNN TNNNNNNNNN N //