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CoralHue(TM) humanized monomeric Azami-Green 1 (phmAG1-S1) (#RDB18249)

CoralHue™ humanized monomeric Azami-Green 1 (phmAG1-S1)

Alternative name AM-V0034M
Clone info. ・For subcloning.
・The codons are humanized.
・Monomeric Azami-Green1 is green fluorescent protein that is stable in acidic and basic conditions without significant loss of signal.
・Ex/Em(nm): 492/505
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Galaxea fascicularis green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18249zzk01.seq provided by the depositor
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Karasawa, S. et al., J. Biol. Chem. 278 (36): 34167-34171, 2003)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18249 CoralHue(TM) humanized monomeric Azami-Green 1 (phmAG1-S1) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) humanized monomeric Azami-Green 1 (phmAG1-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18249).

Reference section:

Karasawa, S., Araki, T., Yamamoto-Hino, M., Miyawaki, A., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18249_B0Khp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: MCS, insert 5'
Sequence file: RDB18249_B0Kha.seq check
>D07039A9_B0Kh_12_lacIpro-B_F_G03_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCGNNTC AGTGAGCGAG GAAGCGGAAG AGCGCCCAAT
   61 ACGCAAACCG CCTCTCCCCG CGCGTTGGCC GATTCATTAA TGCAGCTGGC ACGACAGGTT
  121 TCCCGACTGG AAAGCGGGCA GTGAGCGCAA CGCAATTAAT GTGAGTTAGC TCACTCATTA
  181 GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG
  241 ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GAATTCGAGC TCGGTACCCG
  301 GGGATCCCCA CCATGGTGAG CGTGATCAAG CCCGAGATGA AGATCAAGCT GTGCATGAGG
  361 GGCACCGTGA ACGGCCACAA CTTCGTGATC GAGGGCGAGG GCAAGGGCAA CCCCTACGAG
  421 GGCACCCAGA TCCTGGACCT GAACGTGACC GAGGGCGCCC CCCTGCCCTT CGCCTACGAC
  481 ATCCTGACCA CCGTGTTCCA GTACGGCAAC AGGGCCTTCA CCAAGTACCC CGCCGACATC
  541 CAGGACTACT TCAAGCAGAC CTTCCCCGAG GGCTACCACT GGGAGAGGAG CATGACCTAC
  601 GAGGACCAGG GCATCTGCAC CGCCACCAGC AACATCAGCA TGAGGGGCGA CTGCTTCTTC
  661 TACGACATCA GGTTCGACGG CACCAACTTC CCCCCCAACG GCCCCGTGAT GCAGAAGAAG
  721 ANCCTGAAGT GGGAGCCCAG CACCGAGAAN ATGTACGTGG AGGANGGCGT GCTGAANGGC
  781 GACGTGAACA TGANGCTGCT GCTGGANGGC GGCGGCCACT ACANGTGCGA CTTCNAGACC
  841 ACCTACANNG CCAANAANGA NGTGANGCTG CCCNACGCCC NCAAGATCGA NCACANGATC
  901 GAGANNCTNN ANCACGACAA GGNNNNCAAC NAGGNGNNGC TGNACGANAN NNNNNNNGCN
961 AGGNACTCCA NNCTNNNNNN NNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: MCS, insert 3'
Sequence file: RDB18249_B0Khb.seq check
>D07039A9_B0Kh_12_pUC-M13_-100_H03_08_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CCNGGGTTTT CCCNGTCNCG ACGTTGTAAA
   61 ACGACGGCCA GTGCCAAGCT TGCATGCCTG CAGTTACTTG GCCTGGCTGG GCAGCATGGA
  121 GTACCTGGCC ACGGCGTTCT CGTACAGCTT CACCTTGTTG TAGTCCTTGT CGTGCTTCAG
  181 GATCTCGATC CTGTGGTCGA TCTTGTGGGC GTCGGGCAGC CTCACCTCCT TCTTGGCCTT
  241 GTAGGTGGTC TTGAAGTCGC ACCTGTAGTG GCCGCCGCCC TCCAGCAGCA GCCTCATGTT
  301 CACGTCGCCC TTCAGCACGC CGTCCTCCAC GTACATCTTC TCGGTGCTGG GCTCCCACTT
  361 CAGGGTCTTC TTCTGCATCA CGGGGCCGTT GGGGGGGAAG TTGGTGCCGT CGAACCTGAT
  421 GTCGTAGAAG AAGCAGTCGC CCCTCATGCT GATGTTGCTG GTGGCGGTGC AGATGCCCTG
  481 GTCCTCGTAG GTCATGCTCC TCTCCCAGTG GTAGCCCTCG GGGAAGGTCT GCTTGAAGTA
  541 GTCCTGGATG TCGGCGGGGT ACTTGGTGAA GGCCCTGTTG CCGTACTGGA ACACGGTGGT
  601 CAGGATGTCG TAGGCGAAGG GCAGGGGGGC GCCCTCGGTC ACGTTCAGGT CCAGGATCTG
  661 GGTGCCCTCG TANGGGTTGC CCTTGCCCTC GCCCTCGATC ACGAAGTTGT GGCCGTTCAC
  721 GGTGCCCCTC ATGCACAGCT TGATCTTCAT CTCGGGCTTG ATCACGCTCA CCATGGTGGG
  781 GATCCCCNGG TACCGANCTC GAATTCNTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT
  841 TGTTATCCGC TCACNATTNC ACACAACATA CGAGCCGGAN NCATAAANNN GTAAAGNCTG
  901 GGGNNGNNTA ATGAGTGANC NNACNCACAT TAANTNGCGT NNCGCNCACN GNCCGCTTNN
  961 CNNNCNNGNA AANNTGNCNN GNNNNCNNCN NTNANNGANT CNNNNNNNGC NCNNNNNNNA
1021 NNNNGNNTNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Karasawa, S., A green-emitting fluorescent protein from Galaxeidae coral and its monomeric version for use in fluorescent labeling. J. Biol. Chem. 278 (36): 34167-34171 (2003). PMID 12819206. [link to RRC of NBRP]