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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

CoralHue(TM) humanized monomeric Umikinoko-Green 1 (phmUkG1-S1) (#RDB18307)

CoralHue™ humanized monomeric Umikinoko-Green 1 (phmUkG1-S1)

Alternative name AM-V0164M
Clone info. ・For subcloning.
・The codons are humanized.
・Monomeric Umikinoko-Green1 is brilliant green fluorescent protein and has extremely high pH stability.
・Ex/Em(nm): 483/499
Vector backbone S1 (plasmid)
Size of vector backbone 2.7 kb
Selectable markers Ampicillin
Gene/insert name Sarcophyton sp. green fluorescent protein cDNA
Depositor|Developer MEDICAL & BIOLOGICAL LABORATORIES CO., LTD., | Nakajo, Tomohiro | Karasawa, Satoshi |
 
Sequence (full) RDB18307zzk01.seq provided by the depositor
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Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, citations of literature designated by the DEPOSITOR (Tsutsui, H. et al., Nat. Methods 5 (8): 683-685, 2008) or an acknowledgment to the DEPOSITOR is requested. A RECIPIENT who belongs to a non-profit organization may use the BIOLOGICAL RESOURCE for an academic research that is non-commercial. The RECIPIENT who belongs to commercial organization should obtain written approval from the DEPOSITOR prior to MTA.
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提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Tsutsui, H. et al., Nat. Methods 5 (8): 683-685, 2008)の引用、もしくは、謝辞の表明を必要とする。非営利機関での学術研究目的での利用に限る。営利機関の利用者もしくは商業利用の場合は寄託者から事前にその旨の提供承諾を取得する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18307 CoralHue(TM) humanized monomeric Umikinoko-Green 1 (phmUkG1-S1) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The CoralHue(TM) humanized monomeric Umikinoko-Green 1 (phmUkG1-S1) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18307).

Reference section:

Tsutsui, H., Karasawa, S., Okamura, Y., Miyawaki, A., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18307_B0H4p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: lacIpro-B_F (Pr0724)
Region: MCS, insert 5'
Sequence file: RDB18307_B0H4a.seq check
>D07039H4_B0H4_3_lacIpro-B_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNGCN NCGNNNCNGT GNGCGNGGAA NCGGAAGAGC GCCCAATACG
   61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AGCTGGCACG ACAGGTTTCC
  121 CGACTGGAAA GCGGGCAGTG AGCGCAACGC AATTAATGTG AGTTAGCTCA CTCATTAGGC
  181 ACCCCAGGCT TTACACTTTA TGCTTCCGGC TCGTATGTTG TGTGGAATTG TGAGCGGATA
  241 ACAATTTCAC ACAGGAAACA GCTATGACCA TGATTACGAA TTCGAGCTCG GTACCCGGGG
  301 ATCCCCACCA TGGTGAGCGT GATCAAGGAG GAGATGAAGA TCAAGCTGCA CATGGAGGGC
  361 AACGTGAACG GCCACGCCTT CGTGATCGAG GGCGACGGCA AGGGCAAGCC CTACGACGGC
  421 ACCCAGACCC TGAACCTGAC CGTGAAGGAG GGCGCCCCCC TGCCCTTCAG CTACGACATC
  481 CTGACCAACG CCTTCCAGTA CGGCAACCGC GCCTTCACCA AGTACCCCGC CGACATCCCC
  541 GACTACTTCA AGCAGACCTT CCCCGAGGGC TACAGCTGGG AGCGCACCAT GAGCTACGAG
  601 GACAACGCCA TCTGCAACGT GCGCAGCGAG ATCAGCATGG AGGGCGACTG CTTCATCTAC
  661 AAGATCCGCT TCGACGGCAA GAACTTCCCC CCCAACGGCC CCGTGATGCA GAAGAAGANC
  721 CTGAAGTGGG AGCCCAGCAC CGAGATGATG TACGTGCGCG ACGGCTTCCT GATGGGCGAC
  781 GTGAACATGG CCCTGCTGCT GGANGGCGGC GGCCACCACC GCNGCGACTT CNAGANCAGC
  841 TACAANGCCN ANAANGTGGN GCAGCTGCCC NACNCCCACA AGATCGACCA NNGCATCGAN
  901 ANCCTGANNC ACGACCNCNN NTACNNNNAN GNNNAANCNN NACNNNNNNN CCNNNNNNNN
961 NN
//
Primer: MBL_S1-rev1 (Pr0941)
Region: MCS, insert 3'
Sequence file: RDB18307_B0H4b.seq check
>D07039H4_B0H4_3_MBL_S1-rev1_C09_09_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGGNGNN CGGNGCGGGC CTCTTCGCTA TTACGCCNGC
   61 TGGCGAAAGG GGGNNGTGCT GCAAGGCGAT TAAGTTGGGT AACGCCAGGG TTTTCCCAGT
  121 CACGACGTTG TAAAACGACG GCCAGTGCCA AGCTTGCATG CCTGCAGTTA CTTGCTGGCC
  181 TGGCTGGGCA GCAGGCTGTT GCGGGCCACG GCGTTCTCGT ACAGCTTCAC CTTGCTGTAG
  241 TCGCGGTCGT GGCTCAGGAT CTCGATGCGG TGGTCGATCT TGTGGGCGTC GGGCAGCTGC
  301 ACCACCTTCT TGGCCTTGTA GCTGGTCTTG AAGTCGCAGC GGTGGTGGCC GCCGCCCTCC
  361 AGCAGCAGGG CCATGTTCAC GTCGCCCATC AGGAAGCCGT CGCGCACGTA CATCATCTCG
  421 GTGCTGGGCT CCCACTTCAG GGTCTTCTTC TGCATCACGG GGCCGTTGGG GGGGAAGTTC
  481 TTGCCGTCGA AGCGGATCTT GTAGATGAAG CAGTCGCCCT CCATGCTGAT CTCGCTGCGC
  541 ACGTTGCAGA TGGCGTTGTC CTCGTAGCTC ATGGTGCGCT CCCAGCTGTA GCCCTCGGGG
  601 AAGGTCTGCT TGAAGTAGTC GGGGATGTCG GCGGGGTACT TGGTGAAGGC GCGGTTGCCG
  661 TACTGGAAGG CGTTGGTCAG GATGTCGTAG CTGAAGGGCA GGGGGGCGCC CTCCTTCACG
  721 GTCAGGTTCA GGGTCTGGGT GCCGTCGTAN GGCTTGCCCT TGCCGTCGCC CTCGATCACG
  781 AANGCGTGGC CGTTCACGTT GCCCTCCATG TGCAGCTTGA TCTTCATCTC CTCCTTGATC
  841 ACGCTCACCA TGNNGGGGAN CCCCGGGNAN CNANCTCGAA NTCGTAATCA TGGNCATANC
  901 NGTTTCCTGN GNGAAATNGN TATCNNCTCN NNNNNNCNCA CNANATACNA NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Tsutsui, H., Improving membrane voltage measurements using FRET with new fluorescent proteins. Nat. Methods 5 (8): 683-685 (2008). PMID 18622396. [link to RRC of NBRP]