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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK198

A plasmid for generate yeast lines expressing OsTIR1(wild type) under the GAL promoter.

Catalog number RDB18341
Resource name pMK198
Alternative name GAL-OsTIR1
Clone info. A plasmid for generate yeast lines expressing OsTIR1(wild type) under the GAL promoter, see Fig. 2C and SFig. 4D of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae).
Gene/insert name Oryza sativa OsTIR1 cDNA
Depositor|Developer Kanemaki, Masato |
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External Database
Oryza sativa OsTIR1

          Reference sequence
            
           
          Sequence (full) RDB18341zzk02.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
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          手続きの概要は、「提供申込みについて[link]」をご覧ください。

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          RDB18341 pMK198 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMK198 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18341).

          Reference section:

          Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB18341_B0Gfp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-CAPbs_up_F2 (Pr0739)
          Region: insert 5'(GAL1-10 pro)
          Sequence file: RDB18341_B0Gfa.seq check
          >D07105A9_B0Gf_1_pUC-CAPbs_up_F2_A03_03_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GGCTTNNNNC TTTATGCTTC CGGCTCGTAT GTTGTGTGGA
             61 ATTGTGAGCG GATAACAATT TCACACAGGA AACAGCTATG ACCATGATTA CGCCAAGCTC
            121 GAAATTAACC CTCACTAAAG GGAACAAAAG CTGGTACCGG GCCCCCCCTC GAGGTCGACG
            181 GTATCGATAA GCTTGATATC GAATTCGACA GGTTATCAGC AACAACACAG TCATATCCAT
            241 TCTCAATTAG CTCTACCACA GTGTGTGAAC CAATGTATCC AGCACCACCT GTAACCAAAA
            301 CAATTTTAGA AGTACTTTCA CTTTGTAACT GAGCTGTCAT TTATATTGAA TTTTCAAAAA
            361 TTCTTACTTT TTTTTTGGAT GGACGCAAAG AAGTTTAATA ATCATATTAC ATGGCATTAC
            421 CACCATATAC ATATCCATAT ACATATCCAT ATCTAATCTT ACTTATATGT TGTGGAAATG
            481 TAAAGAGCCC CATTATCTTA GCCTAAAAAA ACCTTCTCTT TGGAACTTTC AGTAATACGC
            541 TTAACTGCTC ATTGCTATAT TGAAGTACGG ATTAGAAGCC GCCGAGCGGG TGACAGCCCT
            601 CCGAAGGAAG ACTCTCCTCC GTGCGTCCTC GTCTTCACCG GTCGCGTTCC TGAAACGCAG
            661 ATGTGCCTCG CGCCGCACTG CTCCGAACAA TAAAGATTCT ACAATACTAG CTTTTATGGT
            721 TATGAAGAGG AAAAATTGGC AGTAACCTGG CCCCACAAAC CTTCNAATGA ACGAATCAAA
            781 TTAACAACCA TAGGATGATA ATGCGATTAG TTTTTTAGCC TTATTTCTGG GGTAATTAAT
            841 CAGCGAAGCG ATGANTTTTG ATCTATTANC AGATATATAA ATGCNAAANC TGCATAACCA
            901 CTTTANCTAA TACTTTCAAC ATTTTCGGNT TGNATTACTT CNNATTCNAN TGNAATAAAA
            961 NTATCNANAA AAAATTGNTN ATATACCTCT ANNCNTTNNN GTCNANGNAN AAAAAACCCN
          1021 NGNNNCNNCN ANTATGANNN NNTTTNCNNG NNNNNNNNNN
          //
          Primer: GAL1pro_F2 (Pr0975)
          Region: insert mid(GAL1-10 pro,OsTIR1 5')
          Sequence file: RDB18341_B0Gfb.seq check
          >D07105A9_B0Gf_1_GAL1pro_F2_A06_01_ABI08.ab1
              1 NNNNNNNNNT TNNNNNTNGN NNTACTTCTT ANNNNNNNGT AATAAAAGTA TCAACAAAAA
             61 ATTGTTAATA TACCTCTATA CTTTAACGTC AAGGAGAAAA AACCCCGGAT CCACTAGTAT
            121 GACTTATTTT CCTGAAGAAG TTGTAGAACA CATATTTTCC TTTTTACCTG CTCAAAGAGA
            181 TAGAAACACC GTCTCCTTGG TCTGTAAGGT TTGGTACGAA ATCGAAAGAT TGTCTAGAAG
            241 AGGTGTTTTT GTTGGTAATT GTTACGCAGT TAGAGCTGGT AGAGTTGCTG CAAGATTCCC
            301 AAACGTTAGA GCATTGACTG TTAAAGGTAA ACCTCATTTT GCTGATTTCA ATTTGGTTCC
            361 ACCTGATTGG GGTGGTTATG CTGGTCCATG GATTGAAGCT GCAGCTAGAG GTTGTCACGG
            421 TTTGGAAGAA TTGAGAATGA AGAGAATGGT TGTTTCTGAT GAATCATTGG AATTGTTAGC
            481 AAGATCTTTT CCTAGATTCA GAGCTTTGGT TTTGATCTCA TGTGAAGGTT TTTCCACCGA
            541 TGGTTTAGCA GCTGTTGCTT CACATTGTAA GTTGTTGAGA GAATTGGATT TGCAAGAAAA
            601 CGAAGTTGAA GATAGAGGTC CAAGATGGTT ATCTTGTTTT CCTGATTCCT GTACAAGTTT
            661 GGTTTCTTTG AACTTCGCAT GTATCAAGGG TGAAGTTAAC GCTGGTAGTT TGGAAAGATT
            721 AGTTTCTAGA TCACCAAATT TGAGATCTTT GAGANTGAAC AGATCCGTTA GTGTTGATAC
            781 CTTGGCAAAG ATCTTGTTGA GAACTCCTAA TTTGGAAGAN TTGGGTACTG GTAACTTGNC
            841 AGANGANTTC NNNACAGAAT CATACTTCAN GTTNANCNNC GCTTTGNAAA AGTGNAAGAN
            901 GNTGANATCT TTGTCAGGNT TTTGGGNNGC ANNNCCNGTT NGNTTGNNNT TTANNNATCC
          961 TTTGNNNGCT CNNNTNGANN GNNTNGNANT TNNNNNTNNN NNNNNNN
          //
          Primer: pBAC_lac_F (Pr0544)
          Region: insert mid(OsTIR1 3')
          Sequence file: RDB18341_B0Gfc.seq check
          >D07105A9_B0Gf_1_pBAC_lac_F_B03_06_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN GNAANANGAC TNNCTATAGG GCGAATTGGA GCTCCNCCGC
             61 GGTGGCGGCC GCTCATAAAA TCTTGACGAA GTTAGGAGCA TCATCTCTGG CACCTGCGGT
            121 TGTTCTGTAG ACGTATAACT TTTCAACTTT AGGCAAGTCA CCATGGTTTT CTTCCATTTC
            181 ATTGGAACCA TCTCTTTCGT TAATAACTTC AACGTTCAAC ATTGGCATCT TTGAAGCTAA
            241 AACTTGACAA CCTTTCAAGG TAACGTTACA ACTGGACATC CACAAAGATC TCATTGTTTC
            301 ATATCTTGCG AAATTACCCA ACAAAGCTGC GTCACCAAAT GGAGAATCTC TGATTTCCAA
            361 CTTTCTTAAG TTCTTACAAC CGTTCATAAC ATGCATCATA CCCTTATCGG AGTCACCAGC
            421 GAATGCGATT GACAACATTT CCAATTGCTT AGCGTATTTA CCGATGTACA TAAAAACCTT
            481 ATCTGTCAAC AAACCACTGA TGGACAATCT TTGCAAACCC TTACACTCTC TAACGATTGC
            541 ACCAAAACCT TCATCTAAAG GTTGTGATGT AACAACATCA GGTTTACCTG GTTCCAAGAT
            601 ACACAATCTG AATCTAGTGA AGTTTGGACA ATTTTTAGCA ACAGTAACCA AAGCTGCGTT
            661 GGTCATTTGA TGACAGAAGT ACAACAAGGA GTTCAACTTA GGACAACCCA ATGAAACTGC
            721 AACTAAACCT TCTTCTGTAA CAGCAGAGTA ACCTGCAACA TAGAAATCAC TTGGAAAAAC
            781 TCTCAATTCT TGCAAATCCT TACAAGAAGA AGCAACAACT TGCAAACCTT TATCTGAGAT
            841 ACAATCNAAA ACCCACAATC TTTGCAACTT AACACATCTA GAGATCATCT TTGTCAAATC
            901 ACTAGCATCN NAAGTTGGNG CGNAAGANAA ATTCAAACCA GTCNATTGAG CACACAAAGG
            961 ANATATAAAA NANAAANNAA CTGGNNTTGN NTCCNNAAAA NNNGNNNAAN ATNNCNNNAT
          1021 NNNANNNTTT TNNNAANNNN ANNNNNNCNT NNNNNNNNNN
          //
          Primer: URA3pro_F (Pr0170)
          Region: insert 3'(URA3 pro, URA3)
          Sequence file: RDB18341_B0Gfd.seq check
          >D07105A9_B0Gf_1_URA3pro_F_D03_12_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGNANNTG CCNNGTATTC TTAACCCAAC TGCACAGAAC
             61 AAAAACCTGC AGGAAACGAA GATAAATCAT GTCGAAAGCT ACATATAAGG AACGTGCTGC
            121 TACTCATCCT AGTCCTGTTG CTGCCAAGCT ATTTAATATC ATGCACGAAA AGCAAACAAA
            181 CTTGTGTGCT TCATTGGATG TTCGTACCAC CAAGGAATTA CTGGAGTTAG TTGAAGCATT
            241 AGGTCCCAAA ATTTGTTTAC TAAAAACACA TGTGGATATC TTGACTGATT TTTCCATGGA
            301 GGGCACAGTT AAGCCGCTAA AGGCATTATC CGCCAAGTAC AATTTTTTAC TCTTCGAAGA
            361 CAGAAAATTT GCTGACATTG GTAATACAGT CAAATTGCAG TACTCTGCGG GTGTATACAG
            421 AATAGCAGAA TGGGCAGACA TTACGAATGC ACACGGTGTG GTGGGCCCAG GTATTGTTAG
            481 CGGTTTGAAG CAGGCGGCAG AAGAAGTAAC AAAGGAACCT AGAGGCCTTT TGATGTTAGC
            541 AGAATTGTCA TGCAAGGGCT CCCTATCTAC TGGAGAATAT ACTAAGGGTA CTGTTGACAT
            601 TGCGAAGAGC GACAAAGATT TTGTTATCGG CTTTATTGCT CAAAGAGACA TGGGTGGAAG
            661 AGATGAAGGT TACGATTGGT TGATTATGAC ACCCGGTGTG GGTTTAGATG ACAAGGGAGA
            721 CGCATTGGGT CAACAGTATA GAACCGTGGA TGATGTGGTC TCTACAGGAT CTGACATTAT
            781 TATTGTTGGA AGAGGACTAT TTGCAAAGGG AAGGGATGCT AAGGTAGAGG GTGAACGTTA
            841 CAGAAAAGCA NGCTGGGAAN CATATTTGAG AAGATGCGGC CAGCAAAACT AAAAAACTGT
            901 ATTATAAGTA AATGNNTGTA TACTAAACTC NNNAATTAGA GCTNNNNNTT AATTATATCA
            961 GTTATTANCC TATGCGGNGT GAAATANCGN NCAGANGCGN AANGAGAAAA NANCNNATCN
           1021 NNAANNGNNA GCNNTNNNNT TTTGNNAAAN TCNNNNNNAN NTTTNNNNNA NNNNNNNNNT
          1081 TTTTTNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

          2024.03.26

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