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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK425 (#RDB18343)

A plasmid for generate yeast lines expressing OsTIR1(F74A) under the GAL promoter.

Alternative name GAL-OsTIR1(F74A)
Clone info. A plasmid for generate yeast lines expressing OsTIR1(F74A) under the GAL promoter, see Fig. 2C and SFig. 4D of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020.
Vector backbone pBluescriptII KS- (plasmid)
Selectable markers Ampicillin (E. coli), URA3 (S. cerevisiae).
Gene/insert name Oryza sativa OsTIR1 cDNA
Depositor|Developer Kanemaki, Masato |
 
Sequence (full) RDB18343zzk02.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18343 pMK425 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMK425 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18343).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18343_B0Gfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2 (Pr0739)
Region: insert 5'(GAL1-10 pro)
Sequence file: RDB18343_B0Gfa.seq check
>D07105B2_B0Gf_2_pUC-CAPbs_up_F2_B07_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNGCTTTNNN CTTNATGCTT CCGGCTCGTA TGTTGTGTGG
   61 AATTGTGAGC GGATAACAAT TTCACACAGG AAACAGCTAT GACCATGATT ACGCCAAGCT
  121 CGAAATTAAC CCTCACTAAA GGGAACAAAA GCTGGTACCG GGCCCCCCCT CGAGGTCGAC
  181 GGTATCGATA AGCTTGATAT CGAATTCGAC AGGTTATCAG CAACAACACA GTCATATCCA
  241 TTCTCAATTA GCTCTACCAC AGTGTGTGAA CCAATGTATC CAGCACCACC TGTAACCAAA
  301 ACAATTTTAG AAGTACTTTC ACTTTGTAAC TGAGCTGTCA TTTATATTGA ATTTTCAAAA
  361 ATTCTTACTT TTTTTTTGGA TGGACGCAAA GAAGTTTAAT AATCATATTA CATGGCATTA
  421 CCACCATATA CATATCCATA TACATATCCA TATCTAATCT TACTTATATG TTGTGGAAAT
  481 GTAAAGAGCC CCATTATCTT AGCCTAAAAA AACCTTCTCT TTGGAACTTT CAGTAATACG
  541 CTTAACTGCT CATTGCTATA TTGAAGTACG GATTAGAAGC CGCCGAGCGG GTGACAGCCC
  601 TCCGAAGGAA GACTCTCCTC CGTGCGTCCT CGTCTTCACC GGTCGCGTTC CTGAAACGCA
  661 GATGTGCCTC GCGCCGCACT GCTCCGAACA ATAAAGATTC TACAATACTA GCTTTTATGG
  721 TTATGAAGAG GAAAAATTGG CAGTAACCTG NCCCCACAAA CCTTCAAATG AACGAATCAA
  781 ATTAACAACC ATAGGATGAT AATGCGATTA GTTTTTTAGC CTTATTTCTG GGGTAATTAA
  841 TCAGCGAANC GATGANTTTT GATCTATTAA CAGATATATA AATGCAAAAA CTGCATNACC
  901 ACTTTAACTA ANACTTTCNA CATTTTCNGN TNGTATTACT TCTTANNCNA ANGNAATAAA
  961 AAGTATCANC NAAAAANNTG NTNANATANC NNCNANACTT TNANNGTNNN NGNANNAAAA
1021 ANNCNNNGNN NNNNNNNN
//
Primer: pBAC_lac_F (Pr0544)
Region: insert mid(OsTIR1(F74A) 3')
Sequence file: RDB18343_B0Gfb.seq check
>D07105B2_B0Gf_2_pBAC_lac_F_C07_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN GNAANANGAC TNNCTATAGG GCGAANTGGA GCTCCNCCGC
   61 GGTGGCGGCC GCTCATAAAA TCTTGACGAA GTTAGGAGCA TCATCTCTGG CACCTGCGGT
  121 TGTTCTGTAG ACGTATAACT TTTCAACTTT AGGCAAGTCA CCATGGTTTT CTTCCATTTC
  181 ATTGGAACCA TCTCTTTCGT TAATAACTTC AACGTTCAAC ATTGGCATCT TTGAAGCTAA
  241 AACTTGACAA CCTTTCAAGG TAACGTTACA ACTGGACATC CACAAAGATC TCATTGTTTC
  301 ATATCTTGCG AAATTACCCA ACAAAGCTGC GTCACCAAAT GGAGAATCTC TGATTTCCAA
  361 CTTTCTTAAG TTCTTACAAC CGTTCATAAC ATGCATCATA CCCTTATCGG AGTCACCAGC
  421 GAATGCGATT GACAACATTT CCAATTGCTT AGCGTATTTA CCGATGTACA TAAAAACCTT
  481 ATCTGTCAAC AAACCACTGA TGGACAATCT TTGCAAACCC TTACACTCTC TAACGATTGC
  541 ACCAAAACCT TCATCTAAAG GTTGTGATGT AACAACATCA GGTTTACCTG GTTCCAAGAT
  601 ACACAATCTG AATCTAGTGA AGTTTGGACA ATTTTTAGCA ACAGTAACCA AAGCTGCGTT
  661 GGTCATTTGA TGACAGAAGT ACAACAAGGA GTTCAACTTA GGACAACCCA ATGAAACTGC
  721 AACTAAACCT TCTTCTGTAA CAGCAGAGTA ACCTGCAACA TAGAAATCAC TTGGAAAAAC
  781 TCTCAATTCT TGCAAATCCT TACAAGAAGA ANCAACAACT TGCAAACCTT TATCTGANAT
  841 ACAATCCAAA ACCCACAATC TTTGCAACTN NACACATCTA GAGNTCATCT TNNTCAAATC
  901 ACTAGCATCC AAAGTTGGTG CGNAAGANAA ANNNNAACNA NNCNANTGAN CACACNAAGG
  961 ATNTATAAAA GANAAACNAA CTGNNNNTNG CATCCCAAAA NNCNGNNNAA NNNNNNNNNN
//
Primer: GAL1pro_F2 (Pr0975)
Region: insert mid(GAL1-10 pro,OsTIR1(F74A) 5')
Sequence file: RDB18343_B0Gfc.seq check
>D07105B2_B0Gf_2_GAL1pro_F2_C06_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNGNTNG NNNTACTTCT TATTCNNATG TAATAAAAGT ATCAACAAAA
   61 AATTGTTAAT ATACCTCTAT ACTTTAACGT CAAGGAGAAA AAACCCCGGA TCCACTAGTA
  121 TGACTTATTT TCCTGAAGAA GTTGTAGAAC ACATATTTTC CTTTTTACCT GCTCAAAGAG
  181 ATAGAAACAC CGTCTCCTTG GTCTGTAAGG TTTGGTACGA AATCGAAAGA TTGTCTAGAA
  241 GAGGTGTTTT TGTTGGTAAT TGTTACGCAG TTAGAGCTGG TAGAGTTGCT GCAAGATTCC
  301 CAAACGTTAG AGCATTGACT GTTAAAGGTA AACCTCATGC TGCTGATTTC AATTTGGTTC
  361 CACCTGATTG GGGTGGTTAT GCTGGTCCAT GGATTGAAGC TGCAGCTAGA GGTTGTCACG
  421 GTTTGGAAGA ATTGAGAATG AAGAGAATGG TTGTTTCTGA TGAATCATTG GAATTGTTAG
  481 CAAGATCTTT TCCTAGATTC AGAGCTTTGG TTTTGATCTC ATGTGAAGGT TTTTCCACCG
  541 ATGGTTTAGC AGCTGTTGCT TCACATTGTA AGTTGTTGAG AGAATTGGAT TTGCAAGAAA
  601 ACGAAGTTGA AGATAGAGGT CCAAGATGGT TATCTTGTTT TCCTGATTCC TGTACAAGTT
  661 TGGTTTCTTT GAACTTCGCA TGTATCAAGG GTGAAGTTAA CGCTGGTAGT TTGGAAAGAT
  721 TAGTTTCTAG ATCACCAAAT TTGAGATCTT TGAGATTGAA CAGATCCGTT AGTGTTGATA
  781 CCTTGGCANA GATCTTGTTG AGAACTCCTA ATTTGGAAGA NTTGGGTANT GGTAACTTGA
  841 CAGATGANTN NNNAACAGAA TCATACTTCA AGTTGANCNC CGCTTTGGAA AAGTGNAANA
  901 NGTTGAGATC NTTGNCANGN TTTNGGGANG NNNGNCCAGT TNGTTNNNCN TTTNNNTATC
961 CNTTNNGNGN NNNNNTNNNN NNGNNTNNNN NTNNNNNNNN NN
//
Primer: URA3pro_F (Pr0170)
Region: insert 3'(URA3 pro, URA3)
Sequence file: RDB18343_B0Gfd.seq check
>D07105B2_B0Gf_2_URA3pro_F_E07_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGANNTGCCN NGTATTCTTA ACCCAACTGC ACAGAACAAA
   61 AACCTGCAGG AAACGAAGAT AAATCATGTC GAAAGCTACA TATAAGGAAC GTGCTGCTAC
  121 TCATCCTAGT CCTGTTGCTG CCAAGCTATT TAATATCATG CACGAAAAGC AAACAAACTT
  181 GTGTGCTTCA TTGGATGTTC GTACCACCAA GGAATTACTG GAGTTAGTTG AAGCATTAGG
  241 TCCCAAAATT TGTTTACTAA AAACACATGT GGATATCTTG ACTGATTTTT CCATGGAGGG
  301 CACAGTTAAG CCGCTAAAGG CATTATCCGC CAAGTACAAT TTTTTACTCT TCGAAGACAG
  361 AAAATTTGCT GACATTGGTA ATACAGTCAA ATTGCAGTAC TCTGCGGGTG TATACAGAAT
  421 AGCAGAATGG GCAGACATTA CGAATGCACA CGGTGTGGTG GGCCCAGGTA TTGTTAGCGG
  481 TTTGAAGCAG GCGGCAGAAG AAGTAACAAA GGAACCTAGA GGCCTTTTGA TGTTAGCAGA
  541 ATTGTCATGC AAGGGCTCCC TATCTACTGG AGAATATACT AAGGGTACTG TTGACATTGC
  601 GAAGAGCGAC AAAGATTTTG TTATCGGCTT TATTGCTCAA AGAGACATGG GTGGAAGAGA
  661 TGAAGGTTAC GATTGGTTGA TTATGACACC CGGTGTGGGT TTAGATGACA AGGGAGACGC
  721 ATTGGGTCAA CAGTATAGAA CCGTGGATGA NGTGGTCTCT ACAGGATCTG ACATTATTAT
  781 TGTTGGAAGA GGANTATTTG CAAAGGGAAN GGANGCTAAN GTAGAGGGTG AANGTTACAG
  841 AAAAGCANGC TGGGAAGCAT ATTTGANAAG ANGCGGCCAG CAAAACTAAA AAAACTGTAT
  901 TATAAGTAAA TGNATGTATA CTAAACTCAC AAATTANAGC TTCNNNTTAA NTATATCAGT
961 TATTANCCTA TGCNGNNGNN NANTACCGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]