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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMK233

A donor vector for introducing CMV-AtAFB2 at the human AAVS1 locus.

Catalog number RDB18347
Resource name pMK233
Alternative name AAVS1 CMV-AtAFB2
Clone info. A donor vector for introducing CMV-AtAFB2 at the human AAVS1 locus, see Fig. 4A and SFig. 6 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020. AtAFB2, a TIR1 paralog in Arabidopsis thaliana.
Vector backbone pCR (plasmid)
Selectable markers Ampicillin (E. coli), Puromycin (mammalian cell).
Gene/insert name Arabidopsis thaliana AFB2 cDNA human AAVS1 genomic
Depositor|Developer Kanemaki, Masato |
Other clones in our bank

External Database
Arabidopsis thaliana AFB2 human AAVS1

        Reference sequence
          
         
        Sequence (full) RDB18347zzk01.seq provided by the depositor

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。
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        手続きの概要は、「提供申込みについて[link]」をご覧ください。

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        RDB18347 pMK233 DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The pMK233 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18347).

        Reference section:

        Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

        Further references such as user reports and related articles (go to bottom)


        Tips

        Featured content

        Featured content Auxin Inducible Degron (AID) System (English text)
        Featured content Auxin Inducible Degron (AID) System (Japanese text)

        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB18347_B0Gfp1-1.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pUC-CAPbs_up_F2 (Pr0739)
        Region: lacO,T3 pro,AAVS1 arm1 5'
        Sequence file: RDB18347_B0Gfa.seq check
        >18347_18347_B0Gf_2_pUC-CAPbs_up_F2_C01_07_ABI24.ab1
            1 NNNNNNNNNN NNNNNCTTNN NNNNTTATGC TTCCGGCTCG TATGTTGTGT GGAATTGTGA
           61 GCGGANAACA ATTTCACACA GGAAACAGCT ATGACCATGA TTACGCCAAG CTCAGAATTA
          121 ACCCTCACTA AAGGGACTAG TCCTGCAGGT TTAAACGAAT TCGCCCTTTG CTTTCTCTGA
          181 CCAGCATTCT CTCCCCTGGG CCTGTGCCGC TTTCTGTCTG CAGCTTGTGG CCTGGGTCAC
          241 CTCTACGGCT GGCCCAGATC CTTCCCTGCC GCCTCCTTCA GGTTCCGTCT TCCTCCACTC
          301 CCTCTTCCCC TTGCTCTCTG CTGTGTTGCT GCCCAAGGAT GCTCTTTCCG GAGCACTTCC
          361 TTCTCGGCGC TGCACCACGT GATGTCCTCT GAGCGGATCC TCCCCGTGTC TGGGTCCTCT
          421 CCGGGCATCT CTCCTCCCTC ACCCAACCCC ATGCCGTCTT CACTCGCTGG GTTCCCTTTT
          481 CCTTCTCCTT CTGGGGCCTG TGCCATCTCT CGTTTCTTAN GATGGCCTTC TCCGACGGAT
          541 GTCTCCCTTG CGTCCCGCCT CCCCTTCTTG TAGGCCTGCA TCATCACCGT TTTTCTGGAC
          601 AACCCCAAAG TACCCCGTCT CCCTGGCTTT AGCCACCTCT CCATCCTCTT GCTTTCTTTG
          661 CCTGGACACC CCGTTCTCCT GTGGATTCNG GTCACCTCTC ACTCCTTTCA TTTGGGCAGC
          721 TCCCCTACCC CCCTTACCTC TCTAGTCTGT GCTAGCTCTT CNNGCCCCNT GTCATGGCAT
          781 CTTCCNNGGG TCCNANAGCT CANCTAGTCT TCTTNCTNCN NNCNNGGNCC CTATGTNNNN
          841 TTNNNNANAN NATGNTNNNN GNNTCNNNGG NTNNNGNGNC NCNNANCTNG NNNNNNNNTN
        901 NNNNTNNCNN GNNNNNNNNN GNNNNNNNNG NNNNNNGNNN NNTTNNNNNN NNNN
        //
        Primer: pUC-M13_-100 (Pr0310)
        Region: T7 pro,AAVS1 arm2 3'
        Sequence file: RDB18347_B0Gfb.seq check
        >18347_18347_B0Gf_2_pUC-M13_-100_D01_10_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NGNTTTCNCN GTCACGACGT TGTAAAACGA CGGCCAGTGA
           61 ATTGTAATAC GACTCACTAT AGGGCGAATT GAATTTAGCG GCCGCGAATT CGCCCTTAGA
          121 GCAGAGCCAG GAACCCCTGT AGGGAAGGGG CAGGAGAGCC AGGGGCATGA GATGGTGGAC
          181 GAGGAAGGGG GACAGGGAAG CCTGAGCGCC TCTCCTGGGC TTGCCAAGGA CTCAAACCCA
          241 GAAGCCCAGA GCAGGGCCTT AGGGAAGCGG GACCCTGCTC TGGGCGGAGG AATATGTCCC
          301 AGATAGCACT GGGGACTCTT TAAGGAAAGA AGGATGGAGA AAGAGAAAGG GAGTAGAGGC
          361 GGCCACGACC TGGTGAACAC CTAGGACGCA CCATTCTCAC AAAGGGAGTT TTCCACACGG
          421 ACACCCCCCT CCTCACCACA GCCCTGCCAG GACGGGGCTG GCTACTGGCC TTATCTCACA
          481 GGTAAAACTG ACGCACGGAG GAACAATATA AATTGGGGAC TAGAAAGGTG AAGAGCCAAA
          541 GTTAGAACTC AGGACCAACT TATTCTGATT TTGTTTTTCN NAACTGCTTC TCCTCTTGGG
          601 AAGTGTAAGG AAGCTGCAGC ACCAGGATCA GTGAAACGCA CCAGACAGCC GCGTCAGANC
          661 AGCTCANGTT CTGGGAGAGG GTAGCGCANG GTGGCCACTG AGAACCGGGC AGGTCACGCA
          721 TCCCCCCCCT TCCCTCCCNN CCCCNGCNNA GCTCTCCCTC CNNGGATCCT CTCTGGNTCC
          781 NTCGNAAGCA AANCNTANNN GNTCTGGNNN GGANAGAGNN GGNTCNNNGA AATGGGGGNG
          841 NGTCNNNNGN NNNNNNATCT GCCNNANNNN NGNNGGGGNN NNNANNNNNN ATCNNNANNN
        901 TANGNANNNN NNNNNN
        //
        Primer: CMV_Forward (Pr0016)
        Region: CMV pro,AtAFB2 5'
        Sequence file: RDB18347_B0Gfc.seq check
        >18347_18347_B0Gf_2_CMV-Forward_E01_13_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN GNNGNNNNNG GGCGGTAGGC GTGTACGGTG GGAGGTCTAT
           61 ATAAGCAGAG CTGGTTTAGT GAACCGTCAG ATCCGCTAGC GCTGCCACCA TGAACTATTT
          121 TCCTGACGAG GTGATTGAGC ACGTCTTTGA CTTCGTGACC AGCCACAAGG ATCGGAACGC
          181 TATTAGCCTG GTGTGCAAGT CATGGTACAA GATCGAGAGA TACTCTAGGC AGAAAGTCTT
          241 CATTGGCAAC TGTTATGCTA TCAACCCCGA GCGGTTGCTG CGGCGGTTCC CCTGCCTGAA
          301 GTCTCTCACC TTGAAGGGGA AGCCCCACTT CGCAGACTTT AACCTGGTGC CACATGAGTG
          361 GGGCGGGTTT GTGCTGCCAT GGATTGAGGC CTTGGCCCGG TCCAGGGTGG GGCTGGAGGA
          421 GCTTAGGCTC AAGAGAATGG TGGTCACCGA TGAGTCCCTG GAGCTGCTCA GCAGGTCCTT
          481 TGTCAACTTC AAGAGCCTCG TGCTCGTCAG CTGCGAGGGG TTCACCACTG ATGGGCTGGC
          541 ATCTATCGCC GCTAACTGTA GACACCTGAG GGATCTCGAT CTGCAGGAAA ATGAAATCGA
          601 CGATCACAGA GGGCAGTGGT TGTCATGTTT CCNCGACACT TGCACTACCC TGGTCACCCT
          661 TAACTTTGCC TGCCTGGAGG GCGAAACCAA CCTGGTGGCC CTCGAACGGC TGGTGGCNNG
          721 GAGCCNAAAC CTCAAATCTT TGAAGCTNNA NCGGGCCGTC CCCCTNNATG CTCTNGNTAG
          781 GTNGATGGCC TGCGCCCCTC NNNNTGTGNA NCTGGGCNTN NNCTCCTANN ANAATGANCC
          841 NGNNTNNNAN NNCTANCTCN NGCTGANNGN NNNNNATCNA NAANNGNNNN AGCTTNNNAN
        901 NNNNGNNNNN NTTNCTNNNN NNCNNCCCNN NNNNNNNN
        //
        Primer: SV40polyA_R2 (Pr0623)
        Region: AtAFB2 3'
        Sequence file: RDB18347_B0Gfd.seq check
        >18347_18347_B0Gf_2_SV40polyA_R2_F01_16_ABI24.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNTTAACNNN NANAATTGCA TTCNTTTTAT GTTTCAGGTT
           61 CAGGGGGAGG TGTGGGAGGT TTTTTAAAGC AAGTAAAACC TCTACAAATG TGGTATGGCT
          121 GATTATGATC TAGAGTCGCG GCCCCGGTGG ATCCCGGGCC CGCGGTACCG TCGACGGGCC
          181 CGTCGGATCC TCACAAAATC CACACAAATG GTGGGGCATC CATCCGTGTC CCGACGACGG
          241 TTCTGTACAG GTACAGCTTA TCGACCTTCT GCCGTCCTTC ATGGCCGTTT TCCTCCATGC
          301 GGTTGTTGTC GTTCTCGTTG ATAATCTCCA CGTTGAGCCA GGGGGCCTTC TCGGCGAGGC
          361 GCTTGCAGCC GGACAGTGTC ACCTCGCAAC TACTCATCCA CAGACTGCGC ATAGTCTCGT
          421 ACTTGCTCAC ATCAGCGAGA AGGGCTGTAT CTCCGAAAGG GCTATCCCGG ATCTCCAACT
          481 TCTTCATTTT CTTGCAGCCG TTGAGCACGT AGAGCATCCC CTTATCTGTA TCGCCAGCAA
          541 AAGCAATGCT CAGCATCTCC AGTTGGTTGG CGTACATCCC AATGTAAAGG AAGACCTGGT
          601 CGGTCAGCAG TCCGGAGAGG CTAAGCCGCC TAANGGACTT GCAGGCCTTG ACGATTGCCC
          661 CGAAGCCCTC GTCCAGGGGC TGANTTGTGA CGTGGTCAGG CTTGTTAGGC TCNAGGANGC
          721 ACAACCTGAA CCTAATAAAG TTGGGGCAGT TCTTGGCCNC TGTCACAAGA NCGGCGTTAG
          781 TCATCTGCTG ACNNAANTAC ANGATGGANT GCAACTTGGG GCATCCNGNN NNNANGGNCN
          841 NCNNNCCNNC CTCTNNNNNN GCTGNGNNNC NNNCCNNNAN NANGNNNNAN GNNANNNNCN
        901 NNANCTNNNN NNNNNN
        //
        Primer: CMVd3_R (Pr0553)
        Region: CMV pro,CMV en,AAVS1 arm1 3'
        Sequence file: RDB18347_B0Gfe.seq check
        >18347_18347_B3BdF_2_CMVd3_R_D01_04_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNTG NNNNNNNGGG CNNNNNNGTT ACGACNTTTT GGAAAGTCCC
           61 GTTGATTTTG GTGCCAAAAC AAACTCCCAT TGACGTCAAT GGGGTGGAGA CTTGGAAATC
          121 CCCGTGAGTC AAACCGCTAT CCACGCCCAT TGATGTACTG CCAAAACCGC ATCACCATGG
          181 TAATAGCGAT GACTAATACG TAGATGTACT GCCAAGTAGG AAAGTCCCAT AAGGTCATGT
          241 ACTGGGCATA ATGCCAGGCG GGCCATTTAC CGTCATTGAC GTCAATAGGG GGCGTACTTG
          301 GCATATGATA CACTTGATGT ACTGCCAAGT GGGCAGTTTA CCGTAAATAC TCCACCCATT
          361 GACGTCAATG GAAAGTCCCT ATTGGCGTTA CTATGGGAAC ATACGTCATT ATTGACGTCA
          421 ATGGGCGGGG GTCGTTGGGC GGTCAGCCAG GCGGGCCATT TACCGTAAGT TATGTAACGC
          481 GGAACTCCAT ATATGGGCTA TGAACTAATG ACCCCGTAAT TGATTACTAT TAATTGCCCC
          541 ACTGTGGGGT GGAGGGGACA GATAAAAGTA CCCAGAACCA GAGCCACATT AACCGGCCCT
          601 GGGAATATAA GGTGGTCCCA GCTCGGGGAC ACAGGATCCC TGGAGGCAGC AAACATGCTG
          661 TCCTGAAGTG GACATAGGGG CCCGGGTTGG AGGAAGAAGA CTAGCTGAGC TCTCGGANCC
          721 CTGGAANATG CNNTGACAGG GGGCTGGAAN ANCTAGCACA GACTANAGAG GTAANGGGGG
          781 TANGGGAGCT GCCNNAATGA AAGGAGTGAN AGGTGANCCG AATCNNNNNG AGAACGGGGT
          841 GTCCAGGCNA AGAAAGCNNN ANGANNNAGA GGTGGCTAAA GCCANGNNAN GGGNNACTTN
          901 GGGGTTNTCC ANAAAAACNG TGATGATGCN NNCTACNANA NNGGGANGNN GGNCNNNNNG
        961 GNNNNNNTNN NNNGNANANN NNNNNNNN
        //
        Primer: hPGKpro_F2 (Pr0952)
        Region: hPGK pro,PuroR,bGH pA
        Sequence file: RDB18347_B0Gff.seq check
        >18347_18347_B3BdF_2_hPGKpro_F2_A01_01_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNCG NNNGTCGGCA GTCGGCTCCC TCGTTGANCG
           61 AATCACCGAC CTCTCTCCCC AGGGGGATCC ACCGGAGCTT ACCATGACCG AGTACAAGCC
          121 CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC GTACGCACCC TCGCCGCCGC
          181 GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG GACCGCCACA TCGAGCGGGT
          241 CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC GACATCGGCA AGGTGTGGGT
          301 CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG GAGAGCGTCG AAGCGGGGGC
          361 GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC GGTTCCCGGC TGGCCGCGCA
          421 GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG GAGCCCGCGT GGTTCCTGGC
          481 CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG GGCAGCGCCG TCGTGCTCCC
          541 CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC CTGGAGACCT CCGCGCCCCG
          601 CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC GCCGACGTCG AGGTGCCCGA
          661 AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC TGAGGTACCC TGTGCCTTCT
          721 AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT CCTTGACCCT GGAAGGTGCC
          781 ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT CGCATTGTCT GANTANGTGT
          841 CATTCTATTC TGGGGGGGTG GGGTGGGGCA NGACAGCANG GGGGAGGANT GGGAAGANNA
          901 TAGCANGCAT GCTGGGGNNG NNGNGGGCTC TATGGNNACT ANGGANNNGA TTGGTGACAN
          961 AAAAGCCCCN TCCNTNNNNC NTNCNTNCTN NCNAGTCTNN NGANNNNNGG NTCTAANCCN
        1021 NNNNNNNNN
        //
        Primer: PuroR_F (Pr0554)
        Region: PuroR 3',bGH pA,AAVS1 arm2 5'
        Sequence file: RDB18347_B0Gfg.seq check
        >18347_18347_B3BdF_2_PuroR_F_B01_02_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NGTGNCNGNA NGNNCGCGCA CCTGGTGCAT GACCCGCAAG
           61 CCCGGTGCCT GAGGTACCCT GTGCCTTCTA GTTGCCAGCC ATCTGTTGTT TGCCCCTCCC
          121 CCGTGCCTTC CTTGACCCTG GAAGGTGCCA CTCCCACTGT CCTTTCCTAA TAAAATGAGG
          181 AAATTGCATC GCATTGTCTG AGTAGGTGTC ATTCTATTCT GGGGGGTGGG GTGGGGCAGG
          241 ACAGCAAGGG GGAGGATTGG GAAGACAATA GCAGGCATGC TGGGGATGCG GTGGGCTCTA
          301 TGGTTACTAG GGACAGGATT GGTGACAGAA AAGCCCCATC CTTAGGCCTC CTCCTTCCTA
          361 GTCTCCTGAT ATTGGGTCTA ACCCCCACCT CCTGTTAGGC AGATTCCTTA TCTGGTGACA
          421 CACCCCCATT TCCTGGAGCC ATCTCTCTCC TTGCCAGAAC CTCTAAGGTT TGCTTACGAT
          481 GGAGCCAGAG AGGATCCTGG GAGGGAGAGC TTGGCAGGGG GTGGGAGGGA AGGGGGGGAT
          541 GCGTGACCTG CCCGGTTCTC AGTGGCCACC CTGCGCTACC CTCTCCCAGA ACCTGAGCTG
          601 CTCTGACGCG GCTGTCTGGT GCGTTTCACT GATCCTGGTG CTGCAGCTTC CTTACACTTC
          661 CCAAGAGGAG AAGCAGTTTG GAAAAACAAA ATCAGAATAA GTTGGTCCTG AGTTCTAACT
          721 TTGGCTCTTC ACCTTTCTAG TCCCCAATTT ATATTGTTCC TCCGTGCGTC AGTTTTACCT
          781 GTGAGATAAN GNCAGTAGCC AGCCCCGTCC TGNCANGGCT GTGGTGANGA GGGGGGTGTC
          841 CGTGTGGAAA ACTCCCTTTG TGANAATGGT GCGTCCTNNG TGTTCACCAG GTCGTGGCCG
          901 CCTCTACTCC CTTTCTCNTN CTNCNTCNNT CNTTCCNTNA AAGANTCCCN NNNTNCTATC
          961 NNGGNNATAT TCCTNCNNCC CNGANNNNGN NNCCNCNTNN CCNTNNNNNC CNNNNNNNGG
        1021 NCNTNNTGGN NTNNNNNNCN NNNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

        2024.03.26

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