RDB18347 - pMK233

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pMK233 (#RDB18347)

A donor vector for introducing CMV-AtAFB2 at the human AAVS1 locus.

Alternative name	AAVS1 CMV-AtAFB2
Clone info.	A donor vector for introducing CMV-AtAFB2 at the human AAVS1 locus, see Fig. 4A and SFig. 6 of Yesbolatova, A. et al., Nat. Commun. 11: 5701, 2020. AtAFB2, a TIR1 paralog in Arabidopsis thaliana.
Vector backbone	pCR (plasmid)
Selectable markers	Ampicillin (E. coli), Puromycin (mammalian cell).
Growth conditions	LB+Amp 37oC
Gene/insert name	Arabidopsis thaliana AFB2 cDNA human AAVS1 genomic
Depositor\|Developer	Kanemaki, Masato \|

Sequence (full)	RDB18347hts01.seq Assembled from experimentally sequenced data.
Publication	Yesbolatova, A., Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]
Test sheet	RDB18347_B3Bop1-2.pdf

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020)).

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Yesbolatova, A. et al., Nat. Commun. 11: 5701 (2020))。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18347	pMK233	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMK233 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18347).

Reference section:

Yesbolatova, A., Saito, Y., Kitamoto, N., Makino-Itou, H., Ajima, R., Nakano, R., Nakaoka, H., Fukui, K., Gamo, K., Tominari, Y., Takeuchi, H., Saga, Y., Hayashi, K., Kanemaki, M.T., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Yesbolatova, A., The auxin-inducible degron 2 technology provides sharp degradation control in yeast, mammalian cells, and mice. Nat. Commun. 11: 5701 (2020). PMID 33177522. [link to RRC of NBRP]