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pcDNA3-FLAG-Crb3ΔCter (#RDB18381)

Expression vector of human Crb3 mutant lacking unique sequence encoded by 3a or 3b at C‐terminus.

Clone info. Expression vector of human crumbs cell polarity complex component 3 (Crb3) mutant lacking unique sequence encoded by 3a or 3b at C‐terminus (delta Cter) tagged with 2xFLAG inserted between Leu40 and Pro41., see Fig.S6C of Iioka, H. et. al, Int. J. Cance, 145 (10): 2740-2753, 2019.
Comment Expression was confirmed by the depositor with immunoblotting.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli)
Gene/insert name human CRB3 cDNA
Depositor|Developer Iioka, Hidekazu |
 
Sequence (full) RDB18381zzk01.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

必要書類
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Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18381 pcDNA3-FLAG-Crb3ΔCter Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-FLAG-Crb3ΔCter was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18381).

Reference section:

Iioka, H., Saito, K., Sakaguchi, M., Tachibana, T., Homma, K., Kondo, E., Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1. Int. J. Cancer 145 (10): 2740-2753 (2019). PMID 30980524. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Iioka, H., Crumbs3 is a critical factor that regulates invasion and metastasis of colon adenocarcinoma via the specific interaction with FGFR1. Int. J. Cancer 145 (10): 2740-2753 (2019). PMID 30980524. [link to RRC of NBRP]