Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pShuttle-CMV-human myosin IIa S1 (#RDB18385)

Expression vector of human skeletal muscle subfragment-1 (S1) of myosin heavy chain 2 (MYH2).

Clone info. Expression vector of human skeletal muscle subfragment-1 (S1) of myosin heavy chain 2 (MYH2), CMV promoter. Construct; MYH2 (Kazusa; Product ID FXC25901) was truncated at Ala849. This fragment included the motor domain, essential light chains (ELC) binding domain and regulatory light chains (RLC) binding domain. The SNAP-tag, FLAG-tag and 6x His-tag were attached at the C-terminal. Two amino acids (Leu-Glu) corresponding to the restriction endonuclease recognition site (XhoI: CTCGAG) were kept between SNAP-tag and FLAG-tag.
Comment Expression was confirmed by the depositor.
Vector backbone pShuttle-CMV (plasmid)
Size of vector backbone 7.5 kb
Selectable markers Kanamycin
Gene/insert name human MYH2 cDNA
Depositor|Developer Iwaki, Mitsuhiro |
 
Sequence (partial) RDB18385pka01.seq provided by the depositor

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Fujita, K. et al., Commun. Biol. 2: 437, 2019)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Fujita, K. et al., Commun. Biol. 2: 437, 2019)。

Catalog # Resource name Availability Shipping form Fee (non-profit org.)
RDB18385 pShuttle-CMV-human myosin IIa S1 Under QC test. Please contact us. DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

How to cite this biological resource

Materials & Methods section:

The pShuttle-CMV-human myosin IIa S1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18385).

Reference section:

Fujita, K., Ohmachi, M., Ikezaki, K., Yanagida, T., Iwaki, M., Direct visualization of human myosin II force generation using DNA origami-based thick filaments. Commun. Biol. 2: 437 (2019). PMID 31799438. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

References

Original, user report and related articles

original Fujita, K., Direct visualization of human myosin II force generation using DNA origami-based thick filaments. Commun. Biol. 2: 437 (2019). PMID 31799438. [link to RRC of NBRP]