Please review the QC test results indicated by icon below as well as clone information before placing your order.

pYC137 pBS-GPDp-DPP1-V5-GFP11-CyC1ter-natNT2

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: V5-GFP11; location: vacuole.

Sequence (full)	RDB18658zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
提供依頼	手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 提供依頼書 [Word] 提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18658_B0Lnp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F2 (Pr0308) Region: f1 ori, T7 pro, GPD pro Sequence file: RDB18658_B0Lna.seq
>D07536A4_B0Ln_2_f1ori_F2_F05_17_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNT NNCGANTTCG NNCTATTGGT TAAAAAAATG AGCTGATTTA    61 ACAAAAATTT AACGCGAATT TTAACAAAAT ATTAACGCTT ACAATTTCCA TTCGCCATTC   121 AGGCTGCGCA ACTGTTGGGA AGGGCGATCG GTGCGGGCCT CTTCGCTATT ACGCCAGCTG   181 GCGAAAGGGG GATGTGCTGC AAGGCGATTA AGTTGGGTAA CGCCAGGGTT TTCCCAGTCA   241 CGACGTTGTA AAACGACGGC CAGTGAGCGC GCGTAATACG ACTCACTATA GGGCGAATTG   301 GGTACCGGGC CCCCCCTCGA GGTCGACGGT ATTGATAAGC TTGATATCGA ATTAGTTTAT   361 CATTATCAAT ACTCGCCATT TCAAAGAATA CGTAAATAAT TAATAGTAGT GATTTTCCTA   421 ACTTTATTTA GTCAAAAAAT TAGCCTTTTA ATTCTGCTGT AACCCGTACA TGCCCAAAAT   481 AGGGGGCGGG TTACACAGAA TATATAACAT CGTAGGTGTC TGGGTGAACA GTTTATTCCT   541 GGCATCCACT AAATATAATG GAGCCCGCTT TTTAAGCTGG CATCCAGAAA AAAAAAGAAT   601 CCCAGCACCA AAATATTGTT TTCTTCACCA ACCATCAGTT CATAGGTCCA TTCTCTTAGC   661 GCAACTACAG AGAACAGGGG CACAAACAGG CAAAAAACGG GCACAACCTC AATGGAGTGA   721 TGCAACCTGC CTGGAGTAAA TGATGACACA AGGCAATTGA CCCACGCATG TATCTATCTC   781 ATTTTCTTAC ACCTTCTATT ACCTTCTGCT CTCTCTGATT TGGAAAAAGC TGAAAAAAAA   841 GGTTGAAACC AGTTCCCTGA AATTATTCCN CTACTTGANT AATANNNATA TNAAGANNGN   901 AGGNANNGAT TGNNATTCTG TAAATCTATT TCTTAAACTT CTNAAATTCT ACTTTTATAG   961 TTAGNCTTTT TTTTNANTTT TAAAANACNN NNNNNTNNNT TNCNANNNAN GCNGNNNNCN  1021 TNNNNNGNNN TTNCNNNTNN NTNNAANNNN NNTTNNNNNN NNNGNNNNNA NNNNNNNNNN //
Primer: pUC-CAPbs_up_F2 (Pr0739) Region: T3 pro, natNT2 (TEF pro, NrsR) Sequence file: RDB18658_B0Lnb.seq
>D07536A4_B0Ln_2_pUC-CAPbs_up_F2_G05_20_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNGCTTN NNNCTTNATG CTTCCGGCTC GTATGTTGTG    61 TGGAATTGTG AGCGGATAAC AATTTCACAC AGGAAACAGC TATGACCATG ATTACGCCAA   121 GCGCGCAATT AACCCTCACT AAAGGGAACA AAAGCTGGAG CTCCACCGCG GTGGCGGCCG   181 CTCTAGAACT AGTGGATCCC GGGTTAATTA AGGCGCGCCA GATCTGTTTA GCTTGCCTCG   241 TCCCCGCCGG GTCACCCGGC CAGCGACATG GAGGCCCAGA ATACCCTCCT TGACAGTCTT   301 GACGTGCGCA GCTCAGGGGC ATGATGTGAC TGTCGCCCGT ACATTTAGCC CATACATCCC   361 CATGTATAAT CATTTGCATC CATACATTTT GATGGCCGCA CGGCGCGAAG CAAAAATTAC   421 GGCTCCTCGC TGCAGACCTG CGAGCAGGGA AACGCTCCCC TCACAGACGC GTTGAATTGT   481 CCCCACGCCG CGCCCCTGTA GAGAAATATA AAAGGTTAGG ATTTGCCACT GAGGTTCTTC   541 TTTCATATAC TTCCTTTTAA AATCTTGCTA GGATACAGTT CTCACATCAC ATCCGAACAT   601 AAACAACCAT GGGTACCACT CTTGACGACA CGGCTTACCG GTACCGCACC AGTGTCCCGG   661 GGGACGCCGA GGCCATCGAG GCACTGGATG GGTCCTTCAC CACCGACACC GTCTTCCGCG   721 TCACCGCCAC CGGGGACGGC TTCACCCTGC GGGAGGTGCC GGTGGANCCG CCCCTGANNA   781 AGGTGTTCCC CGACGACGAA TCGGACGACG AATCGGANGA NGGGGAGGAN GGCGANCCGG   841 ACTCCCGGAN GTTCGTCGCG NACGGGGNNN ANNGCGACCT NNNGGGCTTC GNGNNCGNCT   901 CGNACTCCGG NNNGAANCGN CGGCTGANCG NCNANGANNT CNAGGTCGCC CCNNANCACC   961 NGGGGGCNCG GGGNCNGGCG CGCGTNGANG GGGNNNNNGA NGNNNNNNGN NNGCNANNNG 1021 GNNNNNNGNN NNCNNNGGNN NNANNNNNNN NNN //
Primer: TDH3pro_F (Pr0431) Region: GPD pro, DPP1 5' Sequence file: RDB18658_B0Lnc.seq
>D07536A4_B0Ln_2_TDH3pro_F_E05_14_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TGNNTGNAAT TCTGNNNNNC TATTTCTTAA ACTTCTTAAA    61 TTCTACTTTT ATAGTTAGTC TTTTTTTTAG TTTTAAAACA CCAGAACTTA GTTTCGACGG   121 ATGCGGCCGC ATGAACAGAG TTTCGTTTAT TAAAACGCCT TTCAACATAG GGGCGAAATG   181 GAGATTAGAA GATGTCTTTT TGCTCATTAT CATGATACTT CTTAACTACC CAGTGTATTA   241 CCAACAACCG TTCGAACGTC AGTTTTACAT TAACGATCTC ACTATATCGC ATCCTTATGC   301 GACAACTGAA CGTGTAAATA ACAACATGTT GTTTGTTTAT AGTTTTGTCG TGCCATCTTT   361 AACCATATTG ATAATTGGTT CCATTTTGGC CGATAGAAGA CATTTGATTT TTATTTTGTA   421 CACATCTCTC CTTGGTTTAT CACTCGCTTG GTTCAGTACG AGTTTCTTTA CAAACTTCAT   481 CAAGAATTGG ATTGGAAGAC TAAGACCAGA TTTTCTAGAT CGTTGCCAAC CTGTTGAAGG   541 CTTGCCATTG GACACTTTAT TTACTGCAAA AGATGTGTGT ACGACTAAGA ATCACGAACG   601 TCTGTTGGAT GGGTTTAGGA CAACTCCGTC AGGTCATTCA AGTGAAAGCT TTGCAGGACT   661 GGGTTATTTG TACTTCTGGC TATGTGGGCA ACTTTTGACT GAATCACCGT TGATGCCTTT   721 ATGGAGAAAA ATGGTGGCCT TTCTACCACT GTTAGGAGCT GCACTAATTG CTCTATCCAG   781 AACTCAAGAN TACAGACATC ATTTCGTCGA TGTAATTTTA GGNTCTATGT TGGGTTATAT   841 AATGGCACAC TTTTTCTACA GAAGAATCTT NCCACCCATT GATGATCCTC TTNCGTTCNA   901 ACNNTTGATG GANGATTCNN ANGNCACCCN NGNANGAAGC AGNCACCCNT CNNANGNNCC 961 CNGNANGANG AANTNCATNC TTTGTCCNAN NNANNNNATG NNNNNNNNNN //
Primer: CYC1ter_R (Pr0022) Region: CYC1 ter, GFP11, V5 tag, DPP1 3' Sequence file: RDB18658_B0Lnd.seq
>D07536A4_B0Ln_2_CYC1ter_R_B12_06_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGTG GGGGGNGGGC GTGAATGTAA GCGTGACATA    61 ACTAATTACA TGACTCGAGG TCGACGGTAT CGATAAGCTT GATATCGAAT TCTTAGGTAA   121 TGCCTGCAGC ATTGACATAT TCGTGTAGAA CCATATGATC TCTGCTTGTG GAACCACCAC   181 CACTTCCACC AGTTGAGTCT AAGCCCAAAA GAGGGTTTGG TATGGGTTTC CCAGAACCTC   241 CGGCAGAGGA TCCACTAGTC ATACCTTCAT CGGACAAAGG ATGTAATTCC TCATCCGGGA   301 TCCTCTGATG GGTGACTGCT TCCTCCAGGG TGACATCTGA ATCGTCCATC AATGGTTTGA   361 ACGGAAGAGG ATCATCAATG GGTGGGAAGA TTCTTCTGTA GAAAAAGTGT GCCATTATAT   421 AACCCAACAT AGACCCTAAA ATTACATCGA CGAAATGATG TCTGTAATCT TGAGTTCTGG   481 ATAGAGCAAT TAGTGCAGCT CCTAACAGTG GTAGAAAGGC CACCATTTTT CTCCATAAAG   541 GCATCAACGG TGATTCAGTC AAAAGTTGCC CACATAGCCA GAAGTACAAA TAACCCAGTC   601 CTGCAAAGCT TTCACTTGAA TGACCTGACG GAGTTGTCCT AAACCCATCC AACAGACGTT   661 CGTGATTCTT AGTCGTACAC ACATCTTTTG CAGTAAATAA AGTGTCCAAT GGCAAGCCTT   721 CAACAGGTTG GCAACGATCT AGAAAATCTG GTCTTAGTCT TCCAATCCAA TTCTTGATGA   781 AGTTTGTAAA GAAACTCGTA CTGAACCAAG CGAGTGATAA ACCAAGGAGA GATGTGTACA   841 AAATAAAAAT CAAATGTCTT CTATCGGCCA AAATGGAACC NATTATCAAT ATGGTTAAAG   901 ANGGNNCGAC AAAACTATAA ACAAACAACN TGTTGNTATT NNCACGTTCA GTTGNCGCAT 961 AANGNTGCNA TATANTGAGN TCGNNNATGN AAAACTNNNN TTCNNNNNNN NNN //
Primer: CYC1ter_F (Pr0273) Region: CYC1 ter, natNT2 (ADH1 ter, NrsR) Sequence file: RDB18658_B0Lne.seq
>D07536A4_B0Ln_2_CYC1ter_F_D05_11_ABI24.ab1     1 NNNNNNNNNN NTTNNNNNNN CTCGNNGGNT TTAATTTGCG GCCGAGCTCG ATTACAACAG    61 GTGTTGTCCT CTGAGGACAT AAAATACACA CCGAGATTCA TCAACTCATT GCTGGAGTTA   121 GCATATCTAC AATTGGGTGA AATGGGGAGC GATTTGCAGG CATTTGCTCG GCATGCCGGT   181 AGAGGTGTGG TCAATAAGAG CGACCTCATG CTATACCTGA GAAAGCAACC TGACCTACAG   241 GAAAGAGTTA CTCAAGAATA AGAATTTTCG TTTTAAAACC TAAGAGTCAC TTTAAAATTT   301 GTATACACTT ATTTTTTTTA TAACTTATTT AATAATAAAA ATCATAAATC ATAAGAAATT   361 CGCCTCGAGA TTAGGGGCAG GGCATGCTCA TGTAGAGCGC CTGCTCGCCG TCCGAGGCGG   421 TGCCGTCGTA CAGGGCGGTG TCCAGGCCGC AGAGGGTGAA CCCCATCCGC CGGTACGCGT   481 GGATCGCCGG TGCGTTGACG TTGGTGACCT CCAGCCAGAG GTGCCCGGCG CCCCGCTCGC   541 GGGCGAACTC CGTCGCGAGC CCCATCAACG CGCGCCCGAC CCCGTGCCCC CGGTGCTCCG   601 GGGCGACCTC GATGTCCTCG ACGGTCAGCC GGCGGTTCCA GCCGGAGTAC GAGACGACCA   661 CGAAGCCCGC CAGGTCGCCG TCGTCCCCGT ACGCGACGAA CGTCCGGGAG TCCGGGTCGC   721 CGTCCTCCCC GTCGTCCGAT TCGTCGTCCG ATTCGTCGTC GGGGAACACC TTGGTCAGGG   781 GCGGGTCCAC CGGCACCTCC CGCANGGTGA AGCCGTCCCC GGTGGCGGTG ANGCGGAAGA   841 CGGTGTCGGT GGTGAAGGAN CCATCCNGNG CCTCGATGGN NTCGGCGNCC CCCGGGANAC   901 TGGTGCGGTA NCGGTAAGCC GNGNCNNCNA GANNGGTACC CATGGNTGNT TATGTNCNNN   961 NGTGATGTGA GNNNTGTATC NTANCNNGAN TTNNANNNNN GNANNNNAAA NNNNNNCNNN 1021 NNNNNNANNN NNNNNCNNTT TNNNNNTTNN NNNNNNNGGN NNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl