RDB18661 - pYC144 pBS-GPDp-Pex3N-GFP1-10-CyC1ter-hphMX

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pYC144 pBS-GPDp-Pex3N-GFP1-10-CyC1ter-hphMX (#RDB18661)

The split-GFP system clone for visualizing organelle contact sites in S. cerevisiae. probe: GFP1-10; location: peroxisome.

Alternative name	pYC144, pBS-GPDp-Pex3N-GFP1-10-CyC1ter-hphMX
Clone info.	The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of S. cerevisiae protein channel Pex3p (PEX3) N-terminal 60 a.a fused with GFP1-10 at C-terminus, GPD promoter, CYC1 terminator, hphMX (hygromycin resistant marker).
Vector backbone	pBlueScript SK- (plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Ampicillin (E. coli), Hygromycin B (yeast).
Gene/insert name	S. cerevisiae PEX3 cDNA Aequorea victoria GFP cDNA
Depositor\|Developer	Tamura, Yasushi \|

Sequence (full)	RDB18661zzt01.seq compiled by us

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB18661	pYC144 pBS-GPDp-Pex3N-GFP1-10-CyC1ter-hphMX	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pYC144 pBS-GPDp-Pex3N-GFP1-10-CyC1ter-hphMX was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18661).

Reference section:

Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB18661_B0Lnp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: f1ori_F2 (Pr0308) Region: GPD pro Sequence file: RDB18661_B0Lna.seq
>D07536A7_B0Ln_2_f1ori_F2_F06_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NGNCGANTTC GNNCTATTGG TTAAAAAAAT GAGCTGATTT 61 AACAAAAATT TAACGCGAAT TTTAACAAAA TATTAACGCT TACAATTTCC ATTCGCCATT 121 CAGGCTGCGC AACTGTTGGG AAGGGCGATC GGTGCGGGCC TCTTCGCTAT TACGCCAGCT 181 GGCGAAAGGG GGATGTGCTG CAAGGCGATT AAGTTGGGTA ACGCCAGGGT TTTCCCAGTC 241 ACGACGTTGT AAAACGACGG CCAGTGAGCG CGCGTAATAC GACTCACTAT AGGGCGAATT 301 GGGTACCGGG CCCCCCCTCG AGGTCGACGG TATCGATAAG CTTGATATCG AATTAGTTTA 361 TCATTATCAA TACTCGCCAT TTCAAAGAAT ACGTAAATAA TTAATAGTAG TGATTTTCCT 421 AACTTTATTT AGTCAAAAAA TTAGCCTTTT AATTCTGCTG TAACCCGTAC ATGCCCAAAA 481 TAGGGGGCGG GTTACACAGA ATATATAACA TCGTAGGTGT CTGGGTGAAC AGTTTATTCC 541 TGGCATCCAC TAAATATAAT GGAGCCCGCT TTTTAAGCTG GCATCCAGAA AAAAAAAGAA 601 TCCCAGCACC AAAATATTGT TTTCTTCACC AACCATCAGT TCATAGGTCC ATTCTCTTAG 661 CGCAACTACA GAGAACAGGG GCACAAACAG GCAAAAAACG GGCACAACCT CAATGGAGTG 721 ATGCAACCTG CCTGGAGTAA ATGATGACAC AAGGCAATTG ACCCACGCAT GTATCTATCT 781 CATTTTCTTA CACCTTCTAT TACCTTCTGC TCTCTCTGAT TTGNAAAAAG CTGAAAAAAA 841 AAGGTTGAAA CCAGTTCCCT GAAATTATTC CCNTACTTGA CNNATAAGTA TATAAAGACG 901 GTANGTATTG ATTGNAATTC TGTAAATCTA TTNCTNAAAC TTCTTAAANT CTACTTTTAT 961 AGTTAGNCTT TTTTTNANTT TTAAAACNCC NNANNNTANT TTCNANNNAN GNNGNNNNNN 1021 NNNN //
Primer: reverse (Pr0099) Region: phpMX 5'(TEF pro, HygR 5') Sequence file: RDB18661_B0Lnb.seq
>D07536A7_B0Ln_2_reverse_B01_02_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGNN NNNNNGCTGG AGCTCCNCCG CGGTGGCGGC 61 CGCTCTAGAA CTAGTGGATC CAGTCTTGAC GTGCGCAGCT CAGGGGCATG ATGTGACTGT 121 CGCCCGTACA TTTAGCCCAT ACATCCCCAT GTATAATCAT TTGCATCCAT ACATTTTGAT 181 GGCCGCACGG CGCGAAGCAA AAATTACGGC TCCTCGCTGC GGACCTGCGA GCAGGGAAAC 241 GCTCCCCTCA CAGACGCGTT GAATTGTCCC CACGCCGCGC CCCTGTAGAG AAATATAAAA 301 GGTTAGGATT TGCCACTGAG GTTCTTCTTT CATATACTTC CTTTTAAAAT CTTGCTAGGA 361 TACAGTTCTC ACATCACATC CGAACATAAA CAACCATGGG TAAAAAGCCT GAACTCACCG 421 CGACGTCTGT CGAGAAGTTT CTGATCGAAA AGTTCGACAG CGTCTCCGAC CTGATGCAGC 481 TCTCGGAGGG CGAAGAATCT CGTGCTTTCA GCTTCGATGT AGGAGGGCGT GGATATGTCC 541 TGCGGGTAAA TAGCTGCGCC GATGGTTTCT ACAAAGATCG TTATGTTTAT CGGCACTTTG 601 CATCGGCCGC GCTCCCGATT CCGGAAGTGC TTGACATTGG GGAATTCAGC GAGAGCCTGA 661 CCTATTGCAT CTCCCGCCGT GCACAGGGTG TCACGTTGCA AGACCTGCCT GANNNNNAAC 721 TGCCCGCTGT TCTGCAGCCG GTCGCGGANG CCATGGATGC GATCGCTGCG GCCGATCTTA 781 GCCAGACNAG CGGGTTCGGN CCATTCGGAN CGCANGGAAT CGGTCAATAC ACTACATGGC 841 GTGATTTCAT ATGCGCGATT GNTGATCCCC ATGTGTATCA CTGGCNAACT GTGNTGGANG 901 ACACCGTCAG TGCGTCCGTC GCGCNNNNTC TCGATGANCT GANGCTTTGG GCCGAGGNNT 961 GNCCCNAANN NCGGNACNNC GNGNNNGCNG NANTNNNNNT NCNNCNNNGN NCNGNNNGNN 1021 NNNGGNCNNC NNNNCANNNG NNNNNNGNCT NNNNNNNNGN NNNNNTNNNG GNNNNNNNNN 1081 N //
Primer: TDH3pro_F (Pr0431) Region: GPD pro 3',Pex3N(1-60),GFP1-10 5' Sequence file: RDB18661_B0Lnc.seq
>D07536A7_B0Ln_2_TDH3pro_F_E01_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNGNNA NNTCTNNNNN NCTATTTCTT AAACTTCTTA 61 AATTCTACTT TTATAGTTAG TCTTTTTTTT AGTTTTAAAA CACCAGAACT TAGTTTCGAC 121 GGATGCGGCC GCATGGCCCC AAATCAAAGA TCACGTTCGC TTCTGCAGAG ACATCGAGGA 181 AAGGTACTCA TTTCATTGAC TGGAATAGCT GCTTTATTTA CCACAGGGTC GGTGGTAGTG 241 TTTTTCGTGA AGAGATGGTT GTATAAACAG CAGTTACGGA TTACTGAGCA ACACTTCATC 301 AAAGAACAGA TCGGATCCGG AGGGTCTGGT GGCGGATCAA TGGGTGGCAC TAGTATGAGC 361 AAAGGAGAAG AACTTTTCAC TGGAGTTGTC CCAATTCTTG TTGAATTAGA TGGTGATGTT 421 AATGGGCACA AATTTTCTGT CAGAGGAGAG GGTGAAGGTG ATGCTACAAT CGGAAAACTC 481 ACACTTAAAT TTATTTGCAC TACTGGAAAA CTACCTGTTC CATGGCCAAC ACTTGTCACT 541 ACTCTGACCT ATGGTGTTCA ATGCTTTTCC CGTTATCCGG ATCACATGAA AAGGCATGAC 601 TTTTTCAAGA GTGCCATGCC CGAAGGTTAT GTACAGGAAC GCACTATATC TTTCAAAGAT 661 GACGGGAAAT ACAAGACGCG TGCTGTAGTC AAGTTTGAAG GNGATACCCT TGTTAATCGT 721 ATCGAGTTAA AGGGTACTGA TTTTAAAGAA GATGGAANCA TTCTCGGACA CAAACTCGAG 781 TACAACTTTA ACTCNCACAA TGTATACATC ACGGCAGACA AACNAAANAN NGGANTCAAA 841 GCTAACTTCA CAGTTCGCCA CAACGTTGAA NANGGTTCCG TTNNNCTNGC ANACCNTTAT 901 CNACAAAATA CTNCNATTGG CGATGGNCCN GTCNNTTTNN NANANNACCN TNACNTGNCG 961 ANANNANCTG NCCNTTTCNA AANANCCCNA CGAANNTAAN NANNTNNNNA TCNNGCTNAN 1021 NGNTNCCGNC NNNCNNCNNN NNNNNNN //
Primer: Avgfp-M_F1 (Pr0620) Region: GFP1-10 3',CYC1 ter Sequence file: RDB18661_B0Lnd.seq
>D07536A7_B0Ln_2_Avgfp-M_F1_E02_05_ABI08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNAANNNN NNNNNGCGTG CTGTAGTCAA GTTTGAAGGT 61 GATACCCTTG TTAATCGTAT CGAGTTAAAG GGTACTGATT TTAAAGAAGA TGGAAACATT 121 CTCGGACACA AACTCGAGTA CAACTTTAAC TCACACAATG TATACATCAC GGCAGACAAA 181 CAAAAGAATG GAATCAAAGC TAACTTCACA GTTCGCCACA ACGTTGAAGA TGGTTCCGTT 241 CAACTAGCAG ACCATTATCA ACAAAATACT CCAATTGGCG ATGGCCCTGT CCTTTTACCA 301 GACAACCATT ACCTGTCGAC ACAAACTGTC CTTTCGAAAG ATCCCAACGA AAAGTAAGAA 361 TTCGATATCA AGCTTATCGA TACCGTCGAC CTCGAGTCAT GTAATTAGTT ATGTCACGCT 421 TACATTCACG CCCTCCCCCC ACATCCGCTC TAACCGAAAA GGAAGGAGTT AGACAACCTG 481 AAGTCTAGGT CCCTATTTAT TTTTTTATAG TTATGTTAGT ATTAAGAACG TTATTTATAT 541 TTCAAATTTT TCTTTTTTTT CTGTACAGAC GCGTGTACGC ATGTAACATT ATACTGAAAA 601 CCTTGCTTGA GAAGGTTTTG GGACGCTCGA AGGCTTTAAT TTGCGGCCGG GCAGATGATG 661 TCGAGGCGAA AAAAAATATA AATCACGCTA ACATTTGATT AAAATAGAAC AACTACAATA 721 TAAAAAAACT ATACAAATGA CAAGTTCTTG AAAACAAGAA TCTTTTTATT GTCNGTACTG 781 ATTATTCCTT TGCCCTCGGA NGAGTGCTGG GGCGTCGGTT TCCACTATCG GCGAGNACTT 841 CTACACNGCC ATCGGNNCAG ACGGNCGCGC TNCTGNGGGC GANTTGTGTA CNCCCGANNG 901 TNCCGGCTCC GGATCGGANG ANTGCGTCNC ATCNNCCCTG CGCCCAAGCT GNATCATCGA 961 AATTGNNNTC NNCCNNNCTC TGATANAGNN GGTNNANNAN CNNNGNNNAN CATATNCGCC 1021 CNGNANNCNN CGNNNATCNN NNNNGCNNCN GNANNNNNNN NNN //
Primer: GFP-C (Pr0304) Region: CYC1 ter,phpMX 3'(HygR 3') Sequence file: RDB18661_B0Lne.seq
>D07536A7_B0Ln_2_GFP-C_G03_21_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NTGTCCTTTC GNAAGANCCC NACGAAAAGT 61 AAGAATTCGA TATCAAGNTT ATCGATACCG TCGACCTCGA GTCATGTAAT TAGTTATGTC 121 ACGCTTACAT TCACGCCCTC CCCCCACATC CGCTCTAACC GAAAAGGAAG GAGTTAGACA 181 ACCTGAAGTC TAGGTCCCTA TTTATTTTTT TATAGTTATG TTAGTATTAA GAACGTTATT 241 TATATTTCAA ATTTTTCTTT TTTTTCTGTA CAGACGCGTG TACGCATGTA ACATTATACT 301 GAAAACCTTG CTTGAGAAGG TTTTGGGACG CTCGAAGGCT TTAATTTGCG GCCGGGCAGA 361 TGATGTCGAG GCGAAAAAAA ATATAAATCA CGCTAACATT TGATTAAAAT AGAACAACTA 421 CAATATAAAA AAACTATACA AATGACAAGT TCTTGAAAAC AAGAATCTTT TTATTGTCAG 481 TACTGATTAT TCCTTTGCCC TCGGACGAGT GCTGGGGCGT CGGTTTCCAC TATCGGCGAG 541 TACTTCTACA CAGCCATCGG TCCAGACGGC CGCGCTTCTG CGGGCGATTT GTGTACGCCC 601 GACAGTCCCG GCTCCGGATC GGACGATTGC GTCGCATCGA CCCTGCGCCC AAGCTGCATC 661 ATCGAAATTG CCGTCAACCA AGCTCTGATA GAGTTGGTCA AGACCAATGC GGAGCATATA 721 CGCCCGGAGC CGCGGCGATC CTGCAAGCTC CGGATGCCTC CGCTCGAAGT AGCGCGTCTG 781 CTGCTCCATA CAAGCCAACC ACGGCCTCCA NAAGAAGANG TTGGCGANCT CGTATTNGGA 841 ATCCCCGAAC ATCGCCTCNC TCCAGTCAAT GACCGCTGTT ATGCGGCCAT TGNNNGTCAG 901 GANATTGTNG GANCCNAAAT NCGCNNGCNN NNNGNGNCNN NANTTNNGGG NNNNNNNNNG 961 NNNNANNGNN NTNNNNNNCA TCNNNNNNCN GNNNNNNNNN N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]