Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: V5-GFP1-10; location: endoplasmic reticulum.

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Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18663_B0Lnp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro, insert 5' (hERj1 (1-200), V5 tag) Sequence file: RDB18663_B0Lna.seq
>D07536A9-B0Ln_1_CMV_Forward_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN TGNNGNNNNN GGCGGTAGGC GTGTACGGTG GGAGGTCTAT    61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT   121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA ACTTAAGCTT GGTACCGAGC   181 TCGGATCCGC CACCATGACT GCTCCGTGCT CTCAACCCGC ACAACTGCCA GGACGGAGAC   241 AGCTGGGTCT GGTACCTTTT CCTCCTCCAC CACCACGTAC ACCTCTGCTG TGGTTGCTGC   301 TTCTGCTGCT TGCTGCGGTT GCACCGGCTA GAGGATGGGA ATCCGGCGAT TTGGAGCTGT   361 TTGACCTGGT CGAAGAAGTG CAGTTGAACT TCTACCAGTT TCTCGGGGTA CAACAGGATG   421 CCAGCTCAGC TGATATCCGC AAAGCCTATC GGAAATTGTC CCTCACACTG CATCCCGATA   481 AGAACAAAGA CGAGAATGCC GAAACCCAGT TCAGACAGCT CGTGGCCATT TACGAGGTCC   541 TGAAAGACGA TGAGAGGAGA CAGAGGTATG ACGACATTCT GATCAACGGA CTGCCCGACT   601 GGCGACAGCC TGTGTTCTAC TATAGGCGAG TGCGGAAGAT GTCCAATGCC GAGCTTGCCC   661 TGCTTCTCTT CATCATTCTC ACGGTTGGCC ACTATGCAGT CGTGTGGAGC ATCTACCTGG   721 AGAAACAGCT GGATGAGCTC CTGTCACGCA AGAAGCGCGA AAAGAAGAAG AAAACCGGCA   781 GTAAGAGCGT GGATGGGAAA CCCATACCCA ATCCACTCCT GGGTCTTGAC TCTACTGGAN   841 GGAGTGGCGG CGCGGCCGCC GGTGGCACTA GTATGAGCAA AGGANAANAN CTTTTCNCTG   901 GAGTTGNCCN NATTCTTGNT GAATTAGATG GNGATGTTNN NGGGGCACAA ATTTTCTGTC   961 NNANGAGANG GTGAANGTGA TGCTANNNTC NGAAAACTCA CNNTNNNNTT ATTTGNNCTA 1021 CNNNNAAANN NNCNNNNNNN NNN //
Primer: bGH_rev3 (Pr0885) Region: bGH pA, insert 3' (GFP 1-10) Sequence file: RDB18663_B0Lnb.seq
>D07536A9-B0Ln_1_bGH_rev3_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNNGGNNN NNNACNGANG GCTGGCAACT AGAAGGCACA    61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGATT ACTTTTCGTT GGGATCTTTC   121 GAAAGGACAG TTTGTGTCGA CAGGTAATGG TTGTCTGGTA AAAGGACAGG GCCATCGCCA   181 ATTGGAGTAT TTTGTTGATA ATGGTCTGCT AGTTGAACGG AACCATCTTC AACGTTGTGG   241 CGAACTGTGA AGTTAGCTTT GATTCCATTC TTTTGTTTGT CTGCCGTGAT GTATACATTG   301 TGTGAGTTAA AGTTGTACTC GAGTTTGTGT CCGAGAATGT TTCCATCTTC TTTAAAATCA   361 GTACCCTTTA ACTCGATACG ATTAACAAGG GTATCACCTT CAAACTTGAC TACAGCACGC   421 GTCTTGTATT TCCCGTCATC TTTGAAAGAT ATAGTGCGTT CCTGTACATA ACCTTCGGGC   481 ATGGCACTCT TGAAAAAGTC ATGCCTTTTC ATGTGATCCG GATAACGGGA AAAGCATTGA   541 ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT TCCAGTAGTG   601 CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC TCCTCTGACA   661 GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG   721 AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCNGCGG NCGCGCCGCC ACTCCCTCCA   781 GTAGAGTCAA GANCCANGAN TGGATTGGGT ATGGGTTTCC CATCCNCGCT CTTACTGCCG   841 GTTTTCTTCT TCTTTTCGCG CTTCTTGCGN GANAGGANCT CATCCAGCTG NTTCTCCNNG   901 NANANGCTCC ACACNANTGC NTANNGGNNA ACCGNGAGAA TGANGAANAG AANCNNNNCN   961 ANGNNCNGNN TTNGNNNNTC TTNCNCACNN NNNNANNNTA NAANNCNNGN TGNNGNCNAN 1021 NNNGNNNNNN NNNN //
Primer: paGFP-M_R1 (Pr0893) Region: insert mid (GFP1-10 5', V5 tag, hERj1 (1-200) 3') Sequence file: RDB18663_B0Lnc.seq
>D07536A9-B0Ln_1_paGFP-M_R1_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNTTTTN NTGTGNNCCG GNNAACGGGA AAAGCATTGA    61 ACACCATAGG TCAGAGTAGT GACAAGTGTT GGCCATGGAA CAGGTAGTTT TCCAGTAGTG   121 CAAATAAATT TAAGTGTGAG TTTTCCGATT GTAGCATCAC CTTCACCCTC TCCTCTGACA   181 GAAAATTTGT GCCCATTAAC ATCACCATCT AATTCAACAA GAATTGGGAC AACTCCAGTG   241 AAAAGTTCTT CTCCTTTGCT CATACTAGTG CCACCGGCGG CCGCGCCGCC ACTCCCTCCA   301 GTAGAGTCAA GACCCAGGAG TGGATTGGGT ATGGGTTTCC CATCCACGCT CTTACTGCCG   361 GTTTTCTTCT TCTTTTCGCG CTTCTTGCGT GACAGGAGCT CATCCAGCTG TTTCTCCAGG   421 TAGATGCTCC ACACGACTGC ATAGTGGCCA ACCGTGAGAA TGATGAAGAG AAGCAGGGCA   481 AGCTCGGCAT TGGACATCTT CCGCACTCGC CTATAGTAGA ACACAGGCTG TCGCCAGTCG   541 GGCAGTCCGT TGATCAGAAT GTCGTCATAC CTCTGTCTCC TCTCATCGTC TTTCAGGACC   601 TCGTAAATGG CCACGAGCTG TCTGAACTGG GTTTCGGCAT TCTCGTCTTT GTTCTTATCG   661 GGATGCAGTG TGAGGGACAA TTTCCGATAG GCTTTGCGGA TATCAGCTGA NCTGGCATCC   721 TGTTGTACCC CGAGAAACTG GTAGAAGTTC AACTGCACTT CTTCGACCAG GTCAAACAGC   781 TCCNAAATCG CCGGATTCCC ATCCTCTAGC CGGTGCAACC GCAGCAAGCA GCAGAANNCA   841 GCAACCACAG CAGANGTGTA CGTGGTGGNG GAGGAGGAAA AGGNACCAGA NCNAGCTGTC   901 TNCNNCCNGG NCANTNGTGC GGGTTGANAG CANGGAGNAN TCNTGGNGGN NNGATNCNAN   961 CTCGGTACNN AGCTNAAGTT TAAACNCTAN CCNGCTTNNN CTCCCNNNTA NTGAGTNGNA 1021 NNNANTTTCN NNNAANNCNA GNAANNNNNN GGGNNNNNNN NNN //
Primer: SV40pro_ori_F (Pr0731) Region: SV40 pro_ori, NeoR Sequence file: RDB18663_B0Lnd.seq
>D07536A9-B0Ln_1_SV40pro_ori_F_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNGNCG CCTCTGCCTC TGANCNATTC NNGAAGTAGT GAGGAGGCTT    61 TTTTGGAGGC CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC   121 TGATCAAGAG ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG   181 TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG   241 CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA   301 GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT   361 GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA   421 CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC   481 CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC   541 CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC   601 CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT   661 GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA NCCATGGCGA   721 TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG   781 CCGGCTGGGT GTGGCGGANC GCTATCAGGA CATAGCGTTG GCTACCCGTG ANATTGCTGA   841 AGAGCTTGGC GGCGAATGGG CTGANCGCTT CCTCNNGCTT TACNGNNTCG CCGCNNCCGA   901 TTCNCANCGC ATCGCCTTCT ATCGCCTNCN TGANNAGTTC TTCTGANCGG GANTCTGGGG 961 NTCNAAATGA CCGACCNNGC GACNNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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