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pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10 (#RDB18663)

The split-GFP system clone for visualizing organelle contact sites in mammalian cells. probe: V5-GFP1-10; location: endoplasmic reticulum.

Alternative name pMM86, pcDNA3.1-ERj1N-V5-GFP1-10
Clone info. The split-GFP system clone for visualizing organelle contact sites in vivo. Expression vector of human DnaJ Heat Shock Protein Family (Hsp40) Member C1 (ERj1) N-terminal 200 a.a fused with V5-GFP1-10 at C-terminus.
Vector backbone pcDNA3.1 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin
Gene/insert name human DNAJC1 cDNA Aequorea victoria GFP cDNA
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB18663hts01.seq checkAssembled from experimentally sequenced data.
Publication Tashiro, S., Front. Cell Dev. Biol. 8: 571388 (2020). [link to RRC of NBRP]
Test sheet RDB18663_B1Iap1-2.pdf 
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Tashiro, S. et al. Front. Cell Dev. Biol. 8(1): 6175, 2020)

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18663 pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pMM86 pcDNA3.1-ERj1 1-200-V5-GFP1-10 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18663).

Reference section:

Tashiro, S., Kakimoto, Y., Shinmyo, M., Fujimoto, S., Tamura, Y., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Tashiro, S., Improved Split-GFP Systems for Visualizing Organelle Contact Sites in Yeast and Human Cells. Front. Cell Dev. Biol. 8: 571388 (2020). PMID 33330450. [link to RRC of NBRP]