Please review the QC test results indicated by icon below as well as clone information before placing your order.

pMRX-puro-EGFP-RBD11

Retroviral expression vector for visualization of active Rab11: mouse Rab11-specific binding domain (RBD11) tagged with EGFP.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021). Additional terms and conditions: Regarding pMRX vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literatures for pMX and pMRX vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) is requested.
Ordering	Order form [Credit Card Payment]  [Bank Transfer Payment] MTA, for use for not-for-profit academic purpose [Word] Please visit Information of Request for Distribution.[link]
Remarks	Remember that you will be working with samples containing infectious virus.

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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Osaki, F. et al., J. Cell Sci., 134 (7), jcs.257311, 2021)。 付加的提供条件： pMRXベクターについて：利用者は、研究成果の公表にあたって pMXならびにpMRXベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003; Saitoh T., J. Biol. Chem. 278 (38): 36005-36012, 2003) の引用を必要とする。
提供依頼	提供依頼書 [Word] 提供同意書 (MTA, 非営利学術目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB18788_B1Dup1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352) Region: Pol region,insert 5' Sequence file: RDB18788_B1Dua.seq
>D07827A1_B1Du_3_MMLV-pol_F_A01_01_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNGG NNTCGCAGCT TGGNNACNCG CCGCCCACGT GAAGGCTGCC    61 GACCCCGGGG GTGGNNCATC CTCTAGACTG CCGGATCTGC CATCGATATA AAGCTTGCCA   121 CCATGGTGAG CAAGGGCGAG GAGCTGTTCA CCGGGGTGGT GCCCATCCTG GTCGAGCTGG   181 ACGGCGACGT AAACGGCCAC AAGTTCAGCG TGTCCGGCGA GGGCGAGGGC GATGCCACCT   241 ACGGCAAGCT GACCCTGAAG TTCATCTGCA CCACCGGCAA GCTGCCCGTG CCCTGGCCCA   301 CCCTCGTGAC CACCCTGACC TACGGCGTGC AGTGCTTCAG CCGCTACCCC GACCACATGA   361 AGCAGCACGA CTTCTTCAAG TCCGCCATGC CCGAAGGCTA CGTCCAGGAG CGCACCATCT   421 TCTTCAAGGA CGACGGCAAC TACAAGACCC GCGCCGAGGT GAAGTTCGAG GGCGACACCC   481 TGGTGAACCG CATCGAGCTG AAGGGCATCG ACTTCAAGGA GGACGGCAAC ATCCTGGGGC   541 ACAAGCTGGA GTACAACTAC AACAGCCACA ACGTCTATAT CATGGCCGAC AAGCAGAAGA   601 ACGGCATCAA GGTGAACTTC AAGATCCGCC ACAACATCGA GGACGGCAGC GTGCAGCTCG   661 CCGANCACTA CCAGCAGAAC ACCCCCATCG GCGANGGCCC CGTGCTGCTG CCCGACAACC   721 ACTACCTGAN CACCCAGTCC GCCCTGANCA AAGACCCCNA CGAGAAGCGC GATCACNTGG   781 NCCTGCTNGA NTTCGTGANC GCCGCCNGNN TCACTCTCNG CNNNNNNNAN CTGTANNNNG   841 NTTCNNNNCN GNNTCCNANG NNNNNCTNGN ACNTGAGNNN NNGNGTCNGN NNCNGANANN 901 CNNNNNCTGN NGNNNCNNAN NGNNNANTNN NNNGNNNNNN NNNN //
Primer: ECFP-C_F1 (Pr0925) Region: insert 3',IRES Sequence file: RDB18788_B1Dub.seq
>D07827A1_B1Du_3_ECFP-C_F1_B01_04_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNCGCGNTCA CNTGGTCCTG CTGGAGTTCG TGACCGCCGC    61 CGGGATCACT CTCNGNATGG ACGAGCTGTA CAAGGGTTCA GGCTCGGGAT CCGAGGTGGA   121 ACTTGAACAT GAGGTCAAGC GTCTGAAGCA GGAGAATCAC AAGCTGCGGG ATCAGAATGA   181 TGATTTAAAT GGGCAGATCC TGAGCCTCAG CCTCTACGAG GCGAAGAACC TCTTTGCAAC   241 CCAGACCAAA GCCCAGTCTC TGGCTGCAGA AATCGACCAC AAAGAACTCC TTCGGAGGAA   301 GGACACCCAT ATCCGGGAGC TAGAGGACTA CATTGACAAC CTCCTCGTCA GGGTGATGGC   361 AGAGACACCC AGCATCCTGC GAGTGCCCTA CGAGCCATCC AGAAAAGCTG TATACACCCA   421 GGACCACTTG TCACCCGGGC CACCCCACCT CTGTGGAGAA GCTACTTCAA CCCACTCCTG   481 AAATCACTAG TGAATTCGCG GCCGCTACGT AAATTCCGCC CCCCCCCCTA ACGTTACTGG   541 CCGAAGCCGC TTGGAATAAG GCCGGTGTGC GTTTGTCTAT ATGTTATTTT CCACCATATT   601 GCCGTCTTTT GGCAATGTGA GGGCCCGGAA ACCTGGCCCT GTCTTCTTGA CGAGCATTCC   661 TAGGGGTCTT TCCCCTCTCG CCNAAGGAAT GCAAGGTCTG TTGAATGTCG TGAANGAAGC   721 AGTTCCTCTG GAAGTTTCTT GAANANAAAC AACGTCTGTA GCGACCCTTT GCANGCAGCG   781 GAACCCCCCA CCTGNNNNNN NNTGCCTCTG CGGNCAAAAN CCACGNGTAT NAGANACNCC   841 TGCNAAGGCG GNNCNACCCC ANNGNCNCGT TNTGANTTGG ATANTNGNGG AAAGANTCAA 901 ANNNNNNTNN NNNNGNNNAN TCNNNNNNNN NN //
Primer: EGFP-N_F2 (Pr1228) Region: insert mid Sequence file: RDB18788_B1Duc.seq
>D07827A1_B1Du_3_EGFP-N_F2_C01_07_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNCN NCNTCTTCTT CAGGNNGACG GCAACTACNA GACCCGCGCC    61 GAGGTGAAGT TCGNGGGCGA CACCCTGGTG AACCGCATCG AGCTGAAGGG CATCGACTTC   121 AAGGAGGACG GCAACATCCT GGGGCACAAG CTGGAGTACA ACTACAACAG CCACAACGTC   181 TATATCATGG CCGACAAGCA GAAGAACGGC ATCAAGGTGA ACTTCAAGAT CCGCCACAAC   241 ATCGAGGACG GCAGCGTGCA GCTCGCCGAC CACTACCAGC AGAACACCCC CATCGGCGAC   301 GGCCCCGTGC TGCTGCCCGA CAACCACTAC CTGAGCACCC AGTCCGCCCT GAGCAAAGAC   361 CCCAACGAGA AGCGCGATCA CATGGTCCTG CTGGAGTTCG TGACCGCCGC CGGGATCACT   421 CTCGGCATGG ACGAGCTGTA CAAGGGTTCA GGCTCGGGAT CCGAGGTGGA ACTTGAACAT   481 GAGGTCAAGC GTCTGAAGCA GGAGAATCAC AAGCTGCGGG ATCAGAATGA TGATTTAAAT   541 GGGCAGATCC TGAGCCTCAG CCTCTACGAG GCGAAGAACC TCTTTGCAAC CCAGACCAAA   601 GCCCAGTCTC TGGCTGCAGA AATCGACCAC AAAGAACTCC TTCGGANGAA GGACACCCAT   661 ATCCGGGAGC TAGAGGACTA CATTGACAAC CTCCTCGTCA GGGTGATGGC AGAGACACCC   721 AGCATCCTGC GAGTGCCCTA CGAGCCATCC AGNNNNCTGT ATACACCCAG GACCACTTGT   781 CACCCGGGCC ACNNCNCCTC TGTGGAGAAN CTACTTCAAC CCACTCCTGA AATCACTANN   841 GAATTCNCGN NCGCTANNTA AATTCCGCCC CCCCCCCCTN ACGTTNCTGN NNNAANCCGC   901 TTNNNNNNAN NNNNGNGNNN GNTNGNCTNN ATGNNATTTN NNNCNNTANN GNNNNNNTTT 961 NNNNNNGNNN NGNNNNNNNN NN //
Primer: IRES-F (Pr0036) Region: IRES,PuroR Sequence file: RDB18788_B1Dud.seq
>D07827A1_B1Du_3_IRES-F_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNTNNGNCGA NGTNNNNAAA CGTCTAGGCC CCCCGAACCA    61 CGGGGNCGTG GTTTTCCTTT GAAAAACACG ATGATAATAT GGCCACAACC GTCGACATGA   121 CCGAGTACAA GCCCACGGTG CGCCTCGCCA CCCGCGACGA CGTCCCCCGG GCCGTACGCA   181 CCCTCGCCGC CGCGTTCGCC GACTACCCCG CCACGCGCCA CACCGTCGAC CCGGACCGCC   241 ACATCGAGCG GGTCACCGAG CTGCAAGAAC TCTTCCTCAC GCGCGTCGGG CTCGACATCG   301 GCAAGGTGTG GGTCGCGGAC GACGGCGCCG CGGTGGCGGT CTGGACCACG CCGGAGAGCG   361 TCGAAGCGGG GGCGGTGTTC GCCGAGATCG GCCCGCGCAT GGCCGAGTTG AGCGGTTCCC   421 GGCTGGCCGC GCAGCAACAG ATGGAAGGCC TCCTGGCGCC GCACCGGCCC AAGGAGCCCG   481 CGTGGTTCCT GGCCACCGTC GGCGTCTCGC CCGACCACCA GGGCAAGGGT CTGGGCAGCG   541 CCGTCGTGCT CCCCGGAGTG GAGGCGGCCG AGCGCGCCGG GGTGCCCGCC TTCCTGGAGA   601 CCTCCGCGCC CCGCAACCTC CCCTTCTACG AGCGGCTCGG CTTCACCGTC ACCGCCGACG   661 TCGAGGTGCC CGANNGANCG CGCACCTGGT GCATGACCCG CAAGCCCGGT GCCTGANTCG   721 ACCGCCCGCC CCACGACCCG CAGCGCCCGA CCGAAANGAN CGCACGACCC CNTGGNTCGN   781 CNATAAAATA AAAGANTTTA TTTANTCTNC ANAAAAAGGG GGGAATGAAA GANCCCACCT   841 GTNNNNTTGG NNNGCTAGCN TNAGTNACNN CNTTTTGCNN NGNATGNNAA ANNCNTNNNT 901 GNNNNTNNNN NNNNTCANAN NNNNNNCNNN NNNNNNN //
Primer: MMLV psi_R1 (Pr1054) Region: MMLV psi,ΔU3 5' LTR,CMV en Sequence file: RDB18788_B1Due.seq
>D07827A1_B1Du_3_MMLV psi_R1_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNGNNNNNNN CNGATAAGTT GCTGGCCNGC TTACCTCCCG    61 GTGGTGGGTC GGTGGTCCCT GGGCAGGGGT CTCCCGATCC CGGACGAGCC CCCAAATGAA   121 AGACCCCCGC TGACGGGTAG TCAATCACTC AGAGGAGACC CTCCCAAGGA ACAGCGAGAC   181 CACAAGTCGG ATGCAACTGC AAGAGGGTTT ATTGGATACA CGGGTACCCG GGCGACTCAG   241 TCAATCGGAG GACTGGCGCG CCGAGTGAGG GGTTGTGGGC TCTTTTATTG AGCTCGGGGA   301 GCAGAAGCGC GCGAACAGAA GCGAGAAGCG AACTGATTGG TTAGTTCAAA TAAGGCACAG   361 GGTCATTTCA GGTCCTTGGG GCACCCTGGA AACATCTGAT GGTTCTCTAG CATGGTAATA   421 GCGATGACTA ATACGTAGAT GTACTGCCAA GTAGGAAAGT CCCATAAGGT CATGTACTGG   481 GCATAATGCC AGGCGGGCCA TTTACCGTCA TTGACGTCAA TAGGGGGCGT ACTTGGCATA   541 TGATACACTT GATGTACTGC CAAGTGGGCA GTTTACCGTA AATACTCCAC CCATTGACGT   601 CAATGGAAAG TCCCTATTGG CGTTACTATG GGAACATACG TCATTATTGA CGTCAATGGG   661 CGGGGGTCGT TGGGCGGTCA GCCAGGCGGG CCATTTACCG TAAGTTATGT AACGCGGAAC   721 TCCNTATATG GGCTATGAAC TAATGACCCC GTAATTGATT ACTATTAATA ACTAGTCAAT   781 AATCAATGTC AACATGGCGG TAATGNTGGA NATGANCCNA TATAAATGNA CNTATTATGA   841 TATGGNTACN ACGTATGCNA NGGGNCNNGC TCCTATANNG NAGANNNNNG NNCNTCNNGA   901 TACNCCTATT TTTATNNNNN AANGNCNTGA NNNTAANNGN NTNCNNNNAN NTCNNNNNNN 961 NNTTNNNGGN NANNNNNNNN NN //
Primer: gag-M_F (Pr0471) Region: gag(truncated),pol region,insert 5' Sequence file: RDB18788_B1Duf.seq
>D07827A1_B1Du_3_gag-M_F_G01_19_ABI24.ab1     1 NNNNNNNNNN NNNNNNNTNN NNNNCCCTAA GCCTCCGCCT CCTCTTCCTC CNTCCGCCCC    61 GTCTCTCCCC CTTGAACCTC CTCGTTCGAC CCCGCCTCGA TCCTCCCTTT ATCCAGCCCT   121 CACTCCTTCT CTAGGCGCCC CCATATGGCC ATATGAGATC TTATATGGGG CACCCCCGCC   181 CCTTGTAAAC TTCCCTGACC CTGACATGAC AAGAGTTACT AACAGCCCCT CTCTCCAAGC   241 TCACTTACAG GCTCTCTACT TAGTCCAGCA CGAAGTCTGG AGACCTCTGG CGGCAGCCTA   301 CCAAGAACAA CTGGACCGAC CGGTGGTACC TCACCCTTAC CGAGTCGGCG ACACAGTGTG   361 GGTCCGCCGA CACCAGACTA AGAACCTAGA ACCTCGCTGG AAAGGACCTT ACACAGTCCT   421 GCTGACCACC CCCACCGCCC TCAAAGTAGA CGGCATCGCA GCTTGGATAC ACGCCGCCCA   481 CGTGAAGGCT GCCGACCCCG GGGGTGGACC ATCCTCTAGA CTGCCGGATC TGCCATCGAT   541 ATAAAGCTTG CCACCATGGT GAGCAAGGGC GAGGAGCTGT TCACCGGGGT GGTGCCCATC   601 CTGGTCGAGC TGGACGGCGA CGTAAACGGC CACAAGTTCA GCGTGTCCGG CGAGGGCGAG   661 GGCGATGCCA CCTACGGCAA GCTGACCCTG AAGTTCATCT GCACCACCGG CAAGCTGCCC   721 GTGCCCTGNC CCACCCTCGT GACCACCCTG ACCTACGGCG TGCAGTGCTT CNGCCGCTAC   781 CCCGACCACN TGAAGCAGCA CGACTTCNTC ANGNCCGCCN TGNCCNNANN NTACGTCCAG   841 GANCGCACCN TCTTCTTCNN NNANNANGGN NNNTANNNGA NCNNCNNNNA NGNGNNNTTC 901 GAGGGCNNNN NCNNNNNNNN NN //
Primer: lacIpro-B_F (Pr0724) Region: 3' LTR Sequence file: RDB18788_B1Dug.seq
>D07827A1_B1Du_3_lacIpro-B_F_F01_16_ABI24.ab1     1 NNNNNNNNNN NNNNNNNCGN ANCGNNTCNG TGAGCGAGGN AGCGGAAGAG CGCCCAATAC    61 GCAAACCGCC TCNCCCCGCG CGTTGGCCGA TTCATTAATG CAACTATGGC CATTTAATGT   121 AAATACTTAA GAAAAAAAAC CAAATTAATT TTGATACATG CTGCATGTGA AAGACCCCCG   181 CTGACGGGTA GTCAATCACT CAGAGGAGAC CCTCCCAAGG AACAGCGAGA CCACAAGTCG   241 GATGCAACTG CAAGAGGGTT TATTGGATAC ACGGGTACCC GGGCGACTCA GTCAATCGGA   301 GGACTGGCGC CCCGAGTGAG GGGTTGTGGG CTCTTTTATT GAGCTCGGGG AGCAGAAGCG   361 CGCGAACAGA AGCGAGAAGC GAACTGATTG GTTAGTTCAA ATAAGGCACA GGGTCATTTC   421 AGGTCCTTGG GGCACCCTGG AAACATCTGA TGGTTCTCTA GAAACTGCTG AGGGCTGGAC   481 CGCATCTGGG GACCATCTGT TCTTGGCCCT GAGCCGGGGC AGGAACTGCT TACCACAGAT   541 ATCCTGTTTG GCCCATATTC AGCTGTTCCA TCTGTTCTTG GCCCTGAGCC GGGGCAGGAA   601 CTGCTTACCA CAGATATCCT GTTTGGCCCA TATTCAGCTG TTCCATCTGT TCCTGACCTT   661 GATCTGAACT TCTCTATTCT CAGTTATGTA TTTTTCCATG CCTTGCAAAA TGNCGTTACT   721 TNAGCTAGCT TGCCNAACCT ACAGGTGGGG TCTTTCNTTC CCCCCTTTTT CTGGAGANTA   781 AATAAAATCT TTTANTTTAT CGTCNANCNN TGGGGTCGTG CGCTNCTTNC NGNCNGGCGC   841 TGCNGGNNNN GGGGCGGGCG GTCGANTCNN NANCNGGCTT GNNGGNNATG CNNNNNNNNN 901 N //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2024.03.26

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