Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-AMNN-Puro-mCherry-CTSB

Retroviral expression vector of mouse cathepsin B (CTSB).

Catalog number RDB18953
Resource name pMXs-AMNN-Puro-mCherry-CTSB
Clone info. Retroviral expression vector of mouse cathepsin B (CTSB), mCherry tagged with N-terrminaus of CTSB.
Comment Expression was confirmed by the depositor with immunofluorescence analysis.
Vector backbone pMXs-AMNN-puro (plasmid)
Selectable markers Ampicillin (E. coli), puromycin (mammalian cell)
Gene/insert name mouse CTSB cDNA
Depositor|Developer Higami, Yoshikazu |
Other clones in our bank
External Database
mouse CTSB
          Reference sequence
            
           
          Sequence (full) RDB18953zzk01.seq provided by the depositor

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Mizunoe, Y. et al., Sci. reports, 10 (1): 634, 2020).
          Additional terms and conditions:
          Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          In order to receive this biological resource, please request the Exclusive MTA to us.
          Please visit Information of Request for Distribution.[link] 
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Mizunoe, Y. et al., Sci. reports, 10 (1): 634, 2020)。
          付加的提供条件：
          pMXsシリーズベクターについて：利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 （Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所　北村俊雄博士への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Availability Shipping form Fee (non-profit org.)
          RDB18953 pMXs-AMNN-Puro-mCherry-CTSB Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          How to cite this biological resource

          Materials & Methods section:

          The pMXs-AMNN-Puro-mCherry-CTSB was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18953).

          Reference section:

          Mizunoe, Y., Kobayashi, M., Hoshino, S., Tagawa, R., Itagawa, R., Hoshino, A., Okita, N., Sudo, Y., Nakagawa, Y., Shimano, H., Higami, Y., Cathepsin B overexpression induces degradation of perilipin 1 to cause lipid metabolism dysfunction in adipocytes. Sci. Rep. 10 (1): 634 (2020). PMID 31959889. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)

          Tips

          Featured content

          QC test results

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References

          Original, user report and related articles

          original Mizunoe, Y., Cathepsin B overexpression induces degradation of perilipin 1 to cause lipid metabolism dysfunction in adipocytes. Sci. Rep. 10 (1): 634 (2020). PMID 31959889. [link to RRC of NBRP]

          2023.12.13

          GNP_filter3_RDBDEP_html_231213.pl