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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pCX4-hBSG-c-HA (269) (#RDB18966)

Retroviral expression vector of human BSG.

Clone info. Retroviral expression vector of human basigin (BSG) tagged with HA at C-terminus. Coding 269 aa of BSG-203 (UniProt: P35613-2).
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pCX4-bsr (plasmid)
Size of vector backbone 5.9 kb
Selectable markers Ampicillin (E. coli), Blasticidin S (mammalian cell)
Gene/insert name human BSG cDNA
Depositor|Developer Nagata, Shigekazu |
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Suzuki, J. et al., Proc. Natl. Acad. Sci. USA., 113 (34): 9509–9514, 2016). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
Remarks Remember that you will be working with samples containing infectious virus.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Suzuki, J. et al., Proc. Natl. Acad. Sci. USA., 113 (34): 9509–9514, 2016)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18966 pCX4-hBSG-c-HA (269) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCX4-hBSG-c-HA (269) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18966).

Reference section:

Suzuki, J., Imanishi, E., Nagata, S., Xkr8 phospholipid scrambling complex in apoptotic phosphatidylserine exposure. Proc. Natl. Acad. Sci. U.S.A. 113 (34): 9509-14 (2016). PMID 27503893. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18966_B1Dkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: MMLV-pol_F (Pr0352)
Region: Pol region,insert 5'
Sequence file: RDB18966_B1Dka.seq check
>D07990A6_B1Dk_1_MMLV-pol_F_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNGG NATCGCAGCT TGGNNACNCG CCGCCCACGT NGAAGGCTGC
   61 CGACCCCGGG GGTGGNNCAT CCTCTAGACT GCCGGATCCA TGGCGGCTGC GCTGTTCGTG
  121 CTGCTGGGAT TCGCGCTGCT GGGCACCCAC GGAGCCTCCG GGGCTGCCGG CACAGTCTTC
  181 ACTACCGTAG AAGACCTTGG CTCCAAGATA CTCCTCACCT GCTCCTTGAA TGACAGCGCC
  241 ACAGAGGTCA CAGGGCACCG CTGGCTGAAG GGGGGCGTGG TGCTGAAGGA GGACGCGCTG
  301 CCCGGCCAGA AAACGGAGTT CAAGGTGGAC TCCGACGACC AGTGGGGAGA GTACTCCTGC
  361 GTCTTCCTCC CCGAGCCCAT GGGCACGGCC AACATCCAGC TCCACGGGCC TCCCAGAGTG
  421 AAGGCTGTGA AGTCGTCAGA ACACATCAAC GAGGGGGAGA CGGCCATGCT GGTCTGCAAG
  481 TCAGAGTCCG TGCCACCTGT CACTGACTGG GCCTGGTACA AGATCACTGA CTCTGAGGAC
  541 AAGGCCCTCA TGAACGGCTC CGAGAGCAGG TTCTTCGTGA GTTCCTCGCA GGGCCGGTCA
  601 GAGCTACACA TTGAGAACCT GAACATGGAG GCCGACCCCG GCCAGTACCG GTGCAACGGC
  661 ACCAGCTCCA AGGGCTCCGA CCAGGCCATC ATCACGCTCC GCGTGCGCAG CCACCTGGCC
  721 GCCCTCTGGC CCTTCCTGGG CATCGTGGCT GANGTGCTGG TGCTGGTCAC CATCATCTTC
  781 ATCTACGANA AGCGCCGGAA GCCCGANGAC GTCCTGGATG ATGACGACGC CNGCTCTGCA
  841 CCCCTGAAGA NCAGCGGGCA GCACCANAAT GACAAAGGCA AGAACGTCCG CCAGANGAAC
  901 TCTTNCNAAN TCNTGTACCC NTACNATGTT CCANATTANN CTNGANTCNN GCGGNNNCNG
  961 NNNNNNTTGN NANCTNACNN NNNCTANCNN CNGNNTTNCN NNNANCNGNN NNNNNNNCNN
1021 NNNNNNNN
//
Primer: IRES-R (Pr0037)
Region: IRES,insert 3'(HA,hBSG 3')
Sequence file: RDB18966_B1Dkb.seq check
>D07990A6_B1Dk_1_IRES-R_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGTNA GGGGGGGGGG GCGGANTTGA TCCCGCTAGA
   61 GGGAAACCGT TGCTAGCTTA AGTTAGTTAA CAATTGTTAC CGCGGCCGCT CGAGTCAAGC
  121 GTAATCTGGA ACATCGTATG GGTACATGAA TTCGGAAGAG TTCCTCTGGC GGACGTTCTT
  181 GCCTTTGTCA TTCTGGTGCT GCCCGCTGCT CTTCAGGGGT GCAGAGCCGG CGTCGTCATC
  241 ATCCAGGACG TCCTCGGGCT TCCGGCGCTT CTCGTANATG AAGATGATGG TGACCAGCAC
  301 CAGCACCTCA GCCACGATGC CCAGGAAGGG CCAGAGGGCG GCCAGGTGGC TGCGCACGCG
  361 GAGCGTGATG ATGGCCTGGT CGGAGCCCTT GGAGCTGGTG CCGTTGCACC GGTACTGGCC
  421 GGGGTCGGCC TCCATGTTCA GGTTCTCAAT GTGTAGCTCT GACCGGCCCT GCGAGGAACT
  481 CACGAANAAC CTGCTCTCGG AGCCGTTCAT GAGGGCCTTG TCCTCANAGT CAGTGATCTT
  541 GTACCAGGCC CAGTCAGTGA CAGGTGGCAC GGACTCTGAC TTGCANACCA GCATGGCCGT
  601 CTCCCCCTCG TTGATGTGTT CTGACGACTT CACAGCCTTC ACTCTGGGAG GCCCGTGGAG
  661 CTGGATGTTG GCCGTGCCCA TGGGCTCGGG GAGGAAGACG CAGGAGTACT CTCCCCACTG
  721 GTCGTCGGAG TCCACCTTGA ACTCCGTTTT CTGGCCGGGC AGCGCGTCCT CCTTCAGCAC
  781 NACGCCCCCC TTCAGCCAGC GGTGCCCTGT GANCTCTGTG GCGCTGTCAT TCAAGGAGCA
  841 GGTGANGAGT ATCTNGNANC CAAGGTCTTC TACGGNAGTG ANNANTGTGC CGGNAGCCCC
  901 NGANGNTCCG NGGGNGNCCA GCAGCGCGAA TCCNNGCANC ACGAACAGCG CANCCNCNNT
  961 NNNNNCNGNN AGNCTANANN NNGGNCNNCC CCNNGGGNNN NNNNNCNTNN NNNNNGNNNN
1021 NNNNNN
//
Primer: bsr-C_F (Pr0803)
Region: 3' LTR
Sequence file: RDB18966_B1Dkc.seq check
>D07990A6_B1Dk_1_bsr-C_F_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NNNGTTNNTN NNNNNNNNNN GCANNNNNNT
   61 CAAAACTACG ATTGAAGAAC TCATTCCACT CAAATATACC CGAAATTAAA AGTCGACGAT
  121 AAAATAANNN NNNTTATTTA GTCTCCAGAA AAAGGGGGGA ATGAAAGACC CCACCTGTAG
  181 GTTTGGCAAG CTAGCTTAAG TAACGCCATT TTGCAAGGCA TGGAAAAATA CATAACTGAG
  241 AATAGAGAAG TTCAGATCAA GGTCAGGAAC AGATGGAACA GCTGAATATG GGCCAAACAG
  301 GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCA AGAACAGATG GAACAGCTGA
  361 ATATGGGCCA AACAGGATAT CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC
  421 AGATGGTCCC CAGATGCGGT CCAGCCCTCA GCAGTTTCTA GAGAACCATC AGATGTTTCC
  481 AGGGTGCCCC AAGGACCTGA AATGACCCTG TGCCTTATTT GAACTAACCA ATCAGTTCGC
  541 TTCTCGCTTC TGTTCGCGCG CTTCTGCTCC CCGAGCTCAA TAAAAGAGCC CACAACCCCT
  601 CACTCGGGGC GCCAGTCCTC CGATTGACTG AGTCGCCCGG GTACCCGTGT ATCCAATAAA
  661 CCCTCTTGCA GTTGCATCCG ACTTGTGGTC TCGCTGTTCC TTGGGAGGGT CTCCTCTGAG
  721 TGATTGACTA CCCGTCAGCG GGGGTCTTTC NCATGCAGCA TGTATCAAAA TTAATTTGGT
  781 TTTTTTTCTT AAGNATTTAC ATTAAATGGN CATAGTTGCA NTAATGAATC GGCCNACGCG
  841 CGGGGAGAGG CGGNTTGCGT ATTGGGCGCT CTTCCGCTNN CTCGCTCACN GACTCGCTGC
  901 GCTCGGNNGT TCGGCTGCGN CGAGCGGNAT CNGCTCACTN AANGNNGNNA ATACNNNNNN
  961 TCCNCANAAT CNNGNGNNNN CGCNNNNAAG ANNNTGNNNN NNNANNNNNN GNNANNNNNN
1021 NGNNNCNNNN AANNNCNNNN NNGNNGGCGT TTTTNNNNNN NGNNNNNNNN NN
//
Primer: CMV enh_F1 (Pr0652)
Region: CMV en,5' LTR
Sequence file: RDB18966_B1Dkd.seq check
>18966_18966_B3FfF_1_CMV enh_F1_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNTNNNG CNNNGTANNT GNNCTTATGG GACTTTCCTA CTTGGCAGTA
   61 CATCTACGTA TTAGTCATCG CTATTACCAT GCTAGAGAAC CATCAGATGT TTCCAGGGTG
  121 CCCCAAGGAC CTGAAATGAC CCTGTGCCTT ATTTGAACTA ACCAATCAGT TCGCTTCTCG
  181 CTTCTGTTCG CGCGCTTCTG CTCCCCGAGC TCAATAAAAG AGCCCACAAC CCCTCACTCG
  241 GCGCGCCAGT CCTCCGATTG ACTGAGTCGC CCGGGTACCC GTGTATCCAA TAAACCCTCT
  301 TGCAGTTGCA TCCGACTTGT GGTCTCGCTG TTCCTTGGGA GGGTCTCCTC TGAGTGATTG
361 ACTACCCGTC AGCGGGGGTC TTTCATTTGG GGGNTCNNNN NNNNNN
//
Primer: pMFG_1 (Pr0230)
Region: Pol region,gag(truncated)
Sequence file: RDB18966_B1Dke.seq check
>18966_18966_B3FfF_1_pMFG_1_F01_06_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGTN NNNNNNTCTT GTCNNGTCNG GGTCNGGGAA GTTTACAAGG
   61 GGCGGGGGTG CCCCATATAA GATCTCATAT GGCCATATGG GGGCGCCTAG AGAAGGAGTG
  121 AGGGCTGGAT AAAGGGAGGA TCGAGGCGGG GTCGAACGAG GAGGTTCAAG GGGGAGAGAC
  181 GGGGCGGATG GAGGAAGAGG AGGCGGAGGC TTAGGGTGTA CAAAGGGCTT GACCCAGGGA
  241 GGGGGGTCAA AAGCCAAGGC TTCCCAGGTC ACGATGTAGG GGACCTGGTC TGGGTGTCCA
  301 TGCGGGCCAG GTGAAAAGAC CTTGATCTTA ACCTGGGTGA TGAGGTCTCG GTTAAAGGTG
  361 CCGTCTCGCG GCCATCCGAC GTTAAAGGTT GGCCATTCTG CAGAGCAGAA GGTAACCCAA
  421 CGTCTCTTCT TGACATCTAC CGACTGGTTG TGAGCGATCC GCTCGACATC TTTCCAGTGA
  481 CCTAAGGTCA AACTTAAGGG AGTGGTAANN NNCNGGCCCT AATTTTNNNA NNAATACAGA
  541 AACACNNTCN NACNNANACA ACACAGAACG ATGCTGCANC AGACAAGANN NNNGGCGCGG
601 CTTCGGNCNN NAACCGAAAG NNNNAANTNN NANNGANNNN NNNNNN
//
Primer: IRES-F (Pr0036)
Region: IRES,bsr,3' LTR
Sequence file: RDB18966_B1Dkf.seq check
>D07990A6_B1Dk_1_IRES-F_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGTCGANGTN NNNNNNNNGT CTAGGCCCCC CGAACCNCGG
   61 GGACGTGGTT TTCCTTTGAA AAACACGATA ATACCATGGT CATGAAAACA TTTAACATTT
  121 CTCAACAAGA TCTAGAATTA GTAGAAGTAG CGACAGAGAA GATTACAATG CTTTATGAGG
  181 ATAATAAACA TCATGTGGGA GCGGCAATTC GTACGAAAAC AGGAGAAATC ATTTCGGCAG
  241 TACATATTGA AGCGTATATA GGACGAGTAA CTGTTTGTGC AGAAGCCATT GCGATTGGTA
  301 GTGCAGTTTC GAATGGACAA AAGGATTTTG ACACGATTGT AGCTGTTAGA CACCCTTATT
  361 CTGACGAAGT AGATAGAAGT ATTCGAGTGG TAAGTCCTTG TGGTATGTGT AGGGAGTTGA
  421 TTTCAGACTA TGCACCAGAT TGTTTTGTGT TAATAGAAAT GAATGGCAAG TTAGTCAAAA
  481 CTACGATTGA AGAACTCATT CCACTCAAAT ATACCCGAAA TTAAAAGTCG ACGATAAAAT
  541 AAAAGATTTT ATTTAGTCTC CAGAAAAAGG GGGGAATGAA AGACCCCACC TGTAGGTTTG
  601 GCAAGCTAGC TTAAGTAACG CCATTTTGCA AGGCATGGAA AAATACATAA CTGAGAATAG
  661 AGAAGTTCAG ATCAAGGTCA GGAACAGATG GAACAGCTGA ATATGGGCCA AACAGGATAT
  721 CTGTGGTAAG CAGTTCCTGC CCCGGCTCAG GGCCAAGAAC AGATGGAACA GCTGANTATG
  781 GGCCAAACAG GATATCTGTG GTAAGCAGTT CCTGCCCCGG CTCAGGGCCN AGAACAGATG
  841 GTCCCCAGAT GCGGTCCAGC CCTCAGCAGT TTCTAGAGAN NCATCAGATG TTTCCAGNNN
  901 GCCCCANNGA CCTGNAATGA NCCTGNGCCT TANTTGAACT AACCNATCAG TTCGCTTCTC
  961 NCNTCTGNNC GCGCGNNTCT GNNNCCNNAN CNNNNTAAAA NAGNCNNNNN NCNNNNNNNN
1021 NGGGGNNNNN NNNNN
//
Primer: MMLV psi_F (Pr0863)
Region: MMLV psi,gag(truncated),pol region
Sequence file: RDB18966_B1Dkg.seq check
>D07990A6_B1Dk_1_MMLV psi_F_C01_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNC NNNNNTTTGN TTTCGGTTTG GGANCGAAGC CGCGCCGCGC
   61 GTCTTGTCTG CTGCAGCATC GTTCTGTGTT GTCTCTGTCT GACTGTGTTT CTGTATTTGT
  121 CTGAAAATTA GGGCCAGACT GTTACCACTC CCTTAAGTTT GACCTTAGGT CACTGGAAAG
  181 ATGTCGAGCG GATCGCTCAC AACCAGTCGG TAGATGTCAA GAAGAGACGT TGGGTTACCT
  241 TCTGCTCTGC AGAATGGCCA ACCTTTAACG TCGGATGGCC GCGAGACGGC ACCTTTAACC
  301 GAGACCTCAT CACCCAGGTT AAGATCAAGG TCTTTTCACC TGGCCCGCAT GGACACCCAG
  361 ACCAGGTCCC CTACATCGTG ACCTGGGAAG CCTTGGCTTT TGACCCCCCT CCCTGGGTCA
  421 AGCCCTTTGT ACACCCTAAG CCTCCGCCTC CTCTTCCTCC ATCCGCCCCG TCTCTCCCCC
  481 TTGAACCTCC TCGTTCGACC CCGCCTCGAT CCTCCCTTTA TCCAGCCCTC ACTCCTTCTC
  541 TAGGCGCCCC CATATGGCCA TATGAGATCT TATATGGGGC ACCCCCGCCC CTTGTAAACT
  601 TCCCTGACCC TGACTTGACA AGAGTTACTA ACAGCCCCTC TCTCCAAGCT CACTTACAGG
  661 CTCTCTACTT AGTCCAGCAC GAAGTCTGGA GACCTCTGGC GGCAGCCTAC CAAGAACAAC
  721 TGGACCGACC GGTGGTACCT CACCCTTACC GAGTCGGCGA CACAGTGTGG GTCCGCCGAC
  781 ACCAGACTAA GAACCTAGAA NCTCGCTGGA AAGGANCTTA CACAGTCCTG CTGACCACCC
  841 CCACCGCCCT CAAAGTAGAC GGCATCGCAG CTTGNATACA CGCCNCCCAC NNGAANGCTG
  901 NCNANCCNNG GGGNGGNNNA TCCTCTANAN TGNCNGANCC NTNGNGGCTG CGCTNNNCNN
961 GCTGCNNGGN NTNGCGCTGN NGGGNNNCNN NNNNNCNNNN NGGGGCTGNN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Suzuki, J., Xkr8 phospholipid scrambling complex in apoptotic phosphatidylserine exposure. Proc. Natl. Acad. Sci. U.S.A. 113 (34): 9509-14 (2016). PMID 27503893. [link to RRC of NBRP]