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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Tim1-Fc (#RDB18986)

Mammalian expression vector of the extracellular region of mouse Havcr1 (Tim1) (aa 1-199).

Clone info. Mammalian expression vector of the extracellular region of mouse hepatitis A virus cellular receptor 1 (Havcr1, Aliases: Tim1) (aa 1-199) with a splice donor (GTAAGTC). See Fig.3f of Miyanishi, M. et al. Nature, 450 (7168): 435-439, 2007.
Comment Expression was confirmed by the depositor with Western blotting.
Vector backbone pEF-Fc (plasmid)
Selectable markers Ampicillin
Gene/insert name mouse Havcr1 cDNA
Depositor|Developer Nagata, Shigekazu |
 
Sequence (full) RDB18986zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007). RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is required to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Miyanishi, M. et al., Nature, 450 (7168): 435-439, 2007)。教育・学術研究以外の目的で用いる場合、大阪大学と別途MTAを締結すること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB18986 pEF-Tim1-Fc DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-Tim1-Fc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB18986).

Reference section:

Miyanishi, M., Tada, K., Koike, M., Uchiyama, Y., Kitamura, T., Nagata, S., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB18986_B1Drp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: insert 5'(mTim1(aa 1-199) 5')
Sequence file: RDB18986_B1Dra.seq check
>D07990C5_B1Dr_2_EF1a-C_F_A04_01_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNANTTT GGANCTTGGT TNNTTCTCNA GCCTCAGACA
   61 GTGGTTCAAA GTTTTTTTCT TCCATTTCAG GTGTCGTGAG GAATTCTCTA GAGATCCCTC
  121 GACCTCNAGA TCCATCGGGC CCCCCCTCGA GGTCGACGTC AGTACCATGA ATCAGATTCA
  181 AGTCTTCATT TCAGGCCTCA TACTGCTTCT CCCAGGCGCT GTGGATTCTT ATGTGGAAGT
  241 AAAGGGGGTG GTGGGTCACC CTGTCACACT TCCATGTACT TACTCAACAT ATCGTGGAAT
  301 CACAACGACA TGTTGGGGCC GAGGGCAATG CCCATCTTCT GCTTGTCAAA ATACACTTAT
  361 TTGGACCAAT GGACATCGTG TCACCTATCA GAAGAGCAGT CGGTACAACT TAAAGGGGCA
  421 TATTTCAGAA GGAGATGTGT CCTTGACGAT AGAGAACTCT GTTGAGAGTG ACAGTGGTCT
  481 GTATTGTTGT CGAGTGGAGA TTCCTGGATG GTTTAATGAT CAGAAAGTGA CCTTTTCATT
  541 GCAAGTTAAA CCAGAGATTC CCACACGTCC TCCAAGAAGA CCCACAACTA CAAGGCCCAC
  601 AGCTACAGGA AGACCCACGA CTATTTCAAC AAGATCCACA CATGTACCAA CATCAACCAG
  661 AGTCTCTACC TCCACTCCTC CAACATCTAC ACACACATGG ACTCACAAAC CAGACTGGAA
  721 TGGCACTGTG ACATCCTCAN GAGATACCTG GAGTAATCAC ACTAGTAAGT CGATATCGAA
  781 TTCCTGCAGC CCGGGGGANC CACTAGTTCT ATCCTGCCTG GACGCATCCC GGCTATGCAG
  841 TCCCAGTCCA GGGCANCAAG GCANGNCCCG NCTGCCTCTT CNCCCGGAGG NNTCTGNCCG
  901 CCCCACTCAT GCTCNNGGAN ANGGNCTNCT GGNTTTTTNC CCNNNCTCTG GNNNNNCACN
961 NGCNNNNGCC CCNNNNCNNN NCCNGNNNAN NNNGGGCNNN GCTGGGCTCN NNNNNNNNN
//
Primer: hIgG-genome_R1 (Pr1105)
Region: IgG Fc-region,insert 3'(splice donor site,mTim1(aa1-199) 3')
Sequence file: RDB18986_B1Drb.seq check
>D07990C5_B1Dr_2_hlgG-genome_R1_C04_07_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNGGTC TGANCCCNGC ACCTGCCCCT TTGTGTGCAG
   61 GGCCTGGGTT AGGGGCACCT AGCCTGTGCC TGCCCAGAGC CTGGGGAAAA AGCCAGAAGA
  121 CCCTCTCCCT GAGCATGAGT GGGGCGGGCA GAGGCCTCCG GGTGAAGAGG CAGACGGGGC
  181 CTGCCTTGCT GCCCTGGACT GGGACTGCAT AGCCGGGATG CGTCCAGGCA GGATAGAACT
  241 AGTGGATCCC CCGGGCTGCA GGAATTCGAT ATCGACTTAC TAGTGTGATT ACTCCAGGTA
  301 TCTCCTGAGG ATGTCACAGT GCCATTCCAG TCTGGTTTGT GAGTCCATGT GTGTGTAGAT
  361 GTTGGAGGAG TGGAGGTAGA GACTCTGGTT GATGTTGGTA CATGTGTGGA TCTTGTTGAA
  421 ATAGTCGTGG GTCTTCCTGT AGCTGTGGGC CTTGTAGTTG TGGGTCTTCT TGGAGGACGT
  481 GTGGGAATCT CTGGTTTAAC TTGCAATGAA AAGGTCACTT TCTGATCATT AAACCATCCA
  541 GGAATCTCCA CTCGACAACA ATACAGACCA CTGTCACTCT CAACAGAGTT CTCTATCGTC
  601 AAGGACACAT CTCCTTCTGA AATATGCCCC TTTAAGTTGT ACCGACTGCT CTTCTGATAG
  661 GTGACACGAT GTCCATTGGT CCAAATAAGT GTATTTTGAC AAGCAGAAGA TGGGCATTGC
  721 CCTCGGCCCC AACATGTCGT TGTGATTCCA CGATATGTTG AGTAAGTACA TGGAAGTGTG
  781 ACAGGGTGAC CCACCACCCC CTTTACTTCC NCATAAGAAT CCACAGCGCC TGGGANAAGC
  841 AGTATGAGGC CTGAAATGAA GACTTGAATC TGATTCATGG TACTGACGTC GACCTCNNNG
  901 GGGGGNCCGA TGGATCTCGA GGTCNANGGA TCTCTAGAGA ATTNCTCACG ANNCCTGAAN
  961 TGGAAGAAAA AAANTTTGAN NCNCTGNNTG ANGNTTGANN ATGNANNNNN NATCNNAACT
1021 CNAAANNGNN NNNTNNNNNN ANANTTNCNN NNNNNNNN
//
Primer: hGCSF_R (Pr0031)
Region: hG-CSF 3'UTR+pA,IgG-Fc-region
Sequence file: RDB18986_B1Drc.seq check
>D07990C5_B1Dr_2_hGCSF_R_B04_04_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCCCNG GTGGGGNNCC TCACTCTAGA GTCGCGGCCG
   61 CTCTAGGGCA GTGGTGGGTG CTTTATTTCC ATGCTGGGTG CCTGGGAAGT ATGTAGACGG
  121 GGTACGTGCC AAGCATCCTC GTGCGACCGC GAGAGCCCGG GGAGCGGGGG CTTGCCGGCC
  181 GTCGCACTCA TTTACCCGGG GACAGGGAGA GGCTCTTCTG CGTGTAGTGG TTGTGCAGAG
  241 CCTCATGCAT CACGGAGCAT GAGAAGACGT TCCCCTGCTG CCACCTGCTC TTGTCCACGG
  301 TGAGCTTGCT ATAGAGGAAG AAGGAGCCGT CGGAGTCCAG CACGGGAGGC GTGGTCTTGT
  361 AGTTGTTCTC CGGCTGCCCA TTGCTCTCCC ACTCCACGGC GATGTCGCTG GGATAGAAGC
  421 CTTTGACCAG GCAGGTCAGG CTGACCTGGT TCTTGGTCAT CTCCTCCCGG GATGGGGGCA
  481 GGGTGTACAC CTGTGGTTCT CGGGGCTGCC CTGTAGGGAC AGAGGTTGGT ACAGCGGTCA
  541 CTCTCAGGGC AGAGGGTGGG CCGAGCCGGC CTCTGTCCAT GTGGCCCTCG CACCCCACGG
  601 GTCCCACCTT TGGCTTTGGA GATGGTTTTC TCGATGGGGG CTGGGAGGGC TTTGTTGGAG
  661 ACCTTGCACT TGTACTCCTT GCCATTCAGC CAGTCCTGGT GCAGGACGGT GAGGACGCTG
  721 ACCACACGGT ACGTGCTGTT GTACTGCTCC TCCCGCGGCT TTGTCTTGGC NTTATGCACC
  781 TCCACGCCGT CCACGTACCA GTTGAACTTG ACCTCAGGGT CTTCGTGGCT CACGTCCACC
  841 ACCACGCATG TGACCTCANG GGTCNGGGAN ATCATGANGG TGTCCTTGGG TTTTGGGGGG
  901 GAAGAGGAAG ANTGNNGGTC CCCCNNNGAN TNNAGGTGCT GANGAAGAGA TGGAGGTGGA
  961 NGTGTCAGCA CCCNGNNTGG GGCCTGNNCC TGGATGCNNN NNANTCTNGN NNNNNNNNCC
 1021 NNCNNNGANC NNGNNNGNCG ANNNNTNNNN GGNNTNACNN NNGNNNNNNN GNNNNNNNNN
1081 N
//
Primer: Fc-CH2M_R (Pr1251)
Region: IgG Fc-region
Sequence file: RDB18986_B1Drd.seq check
>D07990C5_B1Dr_2_Fc-CH2M_R_A01_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGNNNNN NNNTGNNNNC ACGGTACGTG CTGTTGTNNC
   61 TGCTCCTCCC GCGGCTTTGT CTTGGCATTA TGCACCTCCA CGCCGTCCAC GTACCAGTTG
  121 AACTTGACCT CAGGGTCTTC GTGGCTCACG TCCACCACCA CGCATGTGAC CTCAGGGGTC
  181 CGGGAGATCA TGAGGGTGTC CTTGGGTTTT GGGGGGAAGA GGAAGACTGA CGGTCCCCCC
  241 AGGAGTTCAG GTGCTGAGGA AGAGATGGAG GTGGACGTGT CAGCACCCGG CTGGGGCCTG
  301 TCCCTGGATG CAGGCTACTC TAGGGCACCT GTCCCGCCTT GAGCTGGAGG GCGAGGCCTG
  361 GGCTGGCTTA CCTGGGCACG GTGGGCATGT GTGAGTTTTG TCACAAGATT TGGGCTCTGC
  421 AGAGAGAAGA TTGGGAGTTA CTGGAATCTG GGAGGAGAGA AGGTGTCCGA GCTGAGGGAG
  481 TGGAGAGTTT GGCCTTTGGG GTGGGCTTAG GTCAGGGGCA GGGTCCTCCC GGATATGGCT
  541 CTTGGCAGGT CTGAGCCCAG CACCTGCCCC TTTGTGTGCA GGGCCTGGGT TAGGGGCACC
  601 TAGCCTGTGC CTGCCCAGAG CCTGGGGAAA AAGCCAGAAG ACCCTCTCCC TGAGCATGAG
  661 TGGGGCGGGC AGAGGCCTCC GGGTGAANAN GCAGACGGGG CCTGCCTTGC TGCCCTGGAC
  721 TGGGACTGCA TAGCCGGGAT GCGTCCNGNN NNGATAGAAC TAGTGGATCC CCCGGGCTGN
  781 NNGAATTCGA TATCGACTTA CTAGTGTGAT TACTCCAGGT ATCTCCTGAN GATGTCNCAG
  841 TGCCATTCCA GTCTGGGTTT GTGAGTCCNT GTGTGTGTAG ATGTNNGANG AGTGGANGTA
  901 NANACTCTGG GTTGATGTTN GGTNCATGTG NNGNATCTTG TTGAAATANT NNNTGGGGNC
  961 NTNCNTGTNN NTGTGGGCNN TGNNANTTNN TGGGGTCNTT CNTNGGANGA NNTGTGGGNA
 1021 NTCTCNGGGT TTNNNNNTNN NNANGAAAAN GNTCNCNTTT NNNNATNNNT TAAANCNNNN
1081 NCNNNGNANT NNNNCNNCNN NNNNNNNN
//
Primer: EF1a-N_R (Pr0024)
Region: SV40 pro_ori,lacO,lac pro
Sequence file: RDB18986_B1Dre.seq check
>D07990C5_B1Dr_2_EF1a-N_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NGGCGNNGTG CGCTCTGCCC NCTGACGGGC ACCGGANCCN
   61 ATTCCCACTC CTTTCAAGAC CTAGAAGGTC CATTAGCTGC AAAGATTCCT CTCTGTTTAA
  121 AACTTTATCC ATCTTTGCAA AGCTTTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC
  181 ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA
  241 TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG
  301 GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC
  361 CTGCTGGGGA GGGAATTAAG CTTGGCGTAA TCATGGTCAT AGCTGTTTCC TGTGTGAAAT
  421 TGTTATCCGC TCACAATTCC ACACAACATA CGAGCCGGAA GCATAAAGTG TAAAGCCTGG
  481 GGTGCCTAAT GAGTGAGCTA ACTCACATTA ATTGCGTTGC GCTCACTGCC CGCTTTCCAG
  541 TCGGGAAACC TGTCGTGCCA GCTGCATTAA TGAATCGGCC AACGCGCGGG GAGAGGCGGT
  601 TTGCGTATTG GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCNNTC GGTCGTTCGG
  661 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG
  721 GANAACGCAG GAAAGAACAT GTGAGCAAAA GGNCANCAAA AGGNCAGGAA CCGTAAAAAG
  781 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAANTCGA
  841 CGCTCNNNTC ANAGGTGGCN AAANCCGACA GGANTATAAN NATANNAGGC GTTTCCCCCT
  901 GGAAGCTCCC TCGTGCGNTC NCCNGTTCCN ANCCTGNCNC TTNNCNGGAT ACNNGNNCNC
  961 CTTTCNNCCC NTNNGNAANC GTNNNGCTTT CTNATANNNC NCGCTGNNNN NATNTNNNNN
1021 NNNGNNGTNN NNNNNTCNNN NNANNNNNGG NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miyanishi, M., Identification of Tim4 as a phosphatidylserine receptor. Nature 450 (7168): 435-439 (2007). PMID 17960135. [link to RRC of NBRP]