Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pMXs-3xFLAG-KLF4(H450A)

Retroviral expression vector of human KLF4, mutant H450A.

Catalog number RDB19010
Resource name pMXs-3xFLAG-KLF4(H450A)
Clone info. Retroviral expression vector of human Kruppel like factor 4 (KLF4) mutant (H450A) tagged with 3xFLAG at N-terminus. This clone was constructed based on pMXs-3xFLAG-KLF4 (RDB19004).
Comment This clone contains a cDNA fragment corresponding to the 627nt to 2037nt of NM_004235.6 (CDS: 598nt to 2037nt) as similar as in the Cell (2007), 131 (5), 861-872. The number at the end of the clone name indicates the position of the substituted amino acid. The position of that amino acid corresponds to NP_001300981.1.
Vector backbone pMXs (plasmid)
Selectable markers Ampicillin
Gene/insert name human KLF4 cDNA
Depositor|Developer Hayashi, Yohei |
Other clones in our bank

External Database
human KLF4

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to DEPOSITOR and a citation of the literature designated by the DEPOSITOR (Borisova, E. et al., iScience 25 (1): 103525 (2021)) are requested. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
          Additional terms and conditions:
          Regarding pMXs series vector: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature for pMXs, pMYs and pMZs vectors (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) and an acknowledgment to Dr. Toshio Kitamura of The Institute of Medical Science, The University of Tokyo are requested.
          Ordering
          Order form [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          Please visit Information of Request for Distribution.[link] 
          Remarks Remember that you will be working with samples containing infectious virus.
          提供案内 (日本国内) [open/close]

          提供条件 利用者は、研究成果の公表にあたって寄託者への謝辞の表明を必要とし、寄託者の指定する文献を引用すること (Borisova, E. et al., iScience 25 (1): 103525 (2021))。本件研究材料は、非営利機関の非営利学術研究に限って提供する。
          付加的提供条件:
          pMXsシリーズベクターについて:利用者は、研究成果の公表にあたって pMXs, pMYs, pMZsベクターの文献 (Kitamura, T., Exp. Hematol. 31 (11): 1007-1014, 2003) を引用し、東京大学医科学研究所 北村俊雄博士への謝辞の表明を必要とする。
          提供依頼
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]
          手続きの概要は、「提供申込みについて[link]」をご覧ください。
          備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB19010 pMXs-3xFLAG-KLF4(H450A) DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pMXs-3xFLAG-KLF4(H450A) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19010).

          Reference section:

          Borisova, E., Nishimura, K., An, Y., Takami, M., Li, J., Song, D., Matsuo-Takasaki, M., Luijkx, D., Aizawa, S., Kuno, A., Sugihara, E., Sato, T.A., Yumoto, F., Terada, T., Hisatake, K., Hayashi, Y., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content


          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19010_B1Ecp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: MMLV-pol_F (Pr0352)
          Region: pol region,insert 5'(3xFLAG,hKLF4(H450A) 5')
          Sequence file: RDB19010_B1Eca.seq check
          >D07716A7_B1Ec_8_MMLV-pol_F_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGG NNTCGCANCT TGGNNACNCG CCGCCCACGN GAAGGCTGCC
             61 GACCCCGGGG GTGGANCATC CTCTAGACTG CCGGATCTAG CTAGTTAATT AAGGATCCCA
            121 GTGTGGTGGT ACGGGAATTC ATGGACTACA AAGACCATGA CGGTGATTAT AAAGATCATG
            181 ACATCGACTA CAAGGATGAC GATGACAAGG CTGTCAGCGA CGCGCTGCTC CCATCTTTCT
            241 CCACGTTCGC GTCTGGCCCG GCGGGAAGGG AGAAGACACT GCGTCAAGCA GGTGCCCCGA
            301 ATAACCGCTG GCGGGAGGAG CTCTCCCACA TGAAGCGACT TCCCCCAGTG CTTCCCGGCC
            361 GCCCCTATGA CCTGGCGGCG GCGACCGTGG CCACAGACCT GGAGAGCGGC GGAGCCGGTG
            421 CGGCTTGCGG CGGTAGCAAC CTGGCGCCCC TACCTCGGAG AGAGACCGAG GAGTTCAACG
            481 ATCTCCTGGA CCTGGACTTT ATTCTCTCCA ATTCGCTGAC CCATCCTCCG GAGTCAGTGG
            541 CCGCCACCGT GTCCTCGTCA GCGTCAGCCT CCTCTTCGTC GTCGCCGTCG AGCAGCGGCC
            601 CTGCCAGCGC GCCCTCCACC TGCAGCTTCA CCTATCCGAT CCGGGCCGGG AACGACCCGG
            661 GCGTGGCGCC GGGCGGCACG GGCGGANGCC TCCTCTATGG CAGGGAGTCC GCTCCCCCTC
            721 CGACGGCTCC CTTCAACCTG GCGGACATCA ACGACGTGAG CCCCTCGGGC GGCTTCGTGG
            781 CCGANCTCCT GCGGCCAGAA TTGGACCCGG TGTACNTTCC GCCGCANCAG CCGCAGCCGC
            841 CAGGTGGCGG GCTGATGGGN AAGTTCGTGC TGAANGNGTC GCTNANCGCC CCTNGNANCG
          901 ANNNNNNNNN N
          //
          Primer: hKLF4-mut check_R2 (Pr1009)
          Region: insert 3'(hKLF4(H450A) 3)',3'LTR
          Sequence file: RDB19010_B1Ecb.seq check
          >D07716A7_B1Ec_8_hKLF4-mut check_R2_E02_14_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNGN NNNNNNNNNN NGGGGCCCNN TTACCCATCC TTCCTGCCCG
             61 ATCAGATGCA GCCGCAAGTC CCGCCGCTCC ATTACCAAGA GCTCATGCCA CCCGGTTCCT
            121 GCATGCCAGA GGAGCCCAAG CCAAAGAGGG GAAGACGATC GTGGCCCCGG AAAAGGACCG
            181 CCACCCACAC TTGTGATTAC GCGGGCTGCG GCAAAACCTA CACAAAGAGT TCCCATCTCA
            241 AGGCAGCCCT GCGAACCCAC ACAGGTGAGA AACCTTACCA CTGTGACTGG GACGGCTGTG
            301 GATGGAAATT CGCCCGCTCA GATGAACTGA CCAGGCACTA CCGTAAACAC ACGGGGCACC
            361 GCCCGTTCCA GTGCCAAAAA TGCGACCGAG CATTTTCCAG GTCGGACCAC CTCGCCTTAC
            421 ACATGAAGAG GCATTTTTAA GCGGCCGCCA GCACAGTGGT CGACGATAAA ATAAAAGATT
            481 TTATTTAGTC TCCAGAAAAA GGGGGGAATG AAAGACCCCA CCTGTAGGTT TGGCAAGCTA
            541 GCTTAAGTAA CGCCATTTTG CAAGGCATGG AAAAATACAT AACTGAGAAT AGAGAAGTTC
            601 AGATCAAGGT CAGGAACAGA TGGAACAGCT GAATATGGGC CAAACAGGAT ATCTGTGGTA
            661 AGCAGTTCCT GCCCCGGCTC ANGGCCAAGA ACAGATGGAA CAGCTGANTA TGGGCCAAAC
            721 AGGATATCTG TGGTAAGCAG TTCCTGCCCC GGCTCANGNC CAAGAACAGA TGGTCCCCAG
            781 ATGCGGTCCA GCCCTCAGCA GTTTCTAGAG AACCATCAGA TGTTTCCAGG GNGCCCCAAG
            841 GNNCTGAANT GANCCTGNGC CNTATTTGAA CTNACNNATC AGTTCGCTTN NCGCTTCNGN
          901 NCGCGCGCNN CTGCTCCCNN NNNNNNNN
          //
          Primer: pMXs-3LTRup_R2 (Pr0365)
          Region: insert mid(hKLF4(H450A) mid)
          Sequence file: RDB19010_B1Ecc.seq check
          >D07716A7_B1Ec_8_pMXs-3LTRup_R2_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNTNNNCGTC GNNNNCTGTG CTGGCGGCCG CTTAAAAATG
             61 CCTCTTCNTG TGTAAGGCGA GGTGGTCCGA CCTGGAAAAT GCTCGGTCGC ATTTTTGGCA
            121 CTGGAACGGG CGGTGCCCCG TGTGTTTACG GTAGTGCCTG GTCAGTTCAT CTGAGCGGGC
            181 GAATTTCCAT CCACAGCCGT CCCAGTCACA GTGGTAAGGT TTCTCACCTG TGTGGGTTCG
            241 CAGGGCTGCC TTGAGATGGG AACTCTTTGT GTAGGTTTTG CCGCAGCCCG CGTAATCACA
            301 AGTGTGGGTG GCGGTCCTTT TCCGGGGCCA CGATCGTCTT CCCCTCTTTG GCTTGGGCTC
            361 CTCTGGCATG CAGGAACCGG GTGGCATGAG CTCTTGGTAA TGGAGCGGCG GGACTTGCGG
            421 CTGCATCTGA TCGGGCAGGA AGGATGGGTA ATTGGGCCCC GGGTGGGGAT GGAAGCCGGG
            481 AGGAAGCGGC AGGGCAGGGT GACAGTCCCT GCTGCTCAGC ACTTCCTCAA GANCCAGGGT
            541 CGGGGTAGTC CTGCTGGGGA GCTGCCGCNN CANGGGGAAN TCGTGTGCAG CCGGCCGGNG
            601 GCCATTGCTG ANANGGGGTC CAGCGCCCAA GTGGGTGCAC NAAGANANNG CCTCCTGCTT
            661 GATCTTGGGG CACGTGCGCN GCGNCCCNNC NNNGNNNNNN GNNNCCACCN CNNGNNNNNN
            721 GNCNNCNNGN NTNNNNTNNC TNANNCTNNN NGANNGANNG NNTNNNNNNN TNNNNNNNNN
          781 GGNNNNNNNN NN
          //
          Primer: gag-N_R (Pr0745)
          Region: MMLV psi,5' LTR
          Sequence file: RDB19010_B1Ecd.seq check
          >D07716A7_B1Ec_8_gag-N_R_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGGN NNNNNTTTTN NGANNAATAC AGAAACNCAG TCAGACAGAG
             61 ACAACACAGA ACGATGCTGC AGCAGACAAG ACGCGCGGCG CGGCTTCGGT CCCAAACCGA
            121 AAGCAAAAAT TCAGACGGAG GCGGGAACTG TTTTAGGTTC TCGTCTCCTA CCAGAACCAC
            181 ATATCCCTCC TATTAGGGGG GTGCACCAAA GAGTCCAAAA CGATCGGGAT TTTTGGACTC
            241 AGGTCGGGCC ACAAAAACGG CCCCCGAAGT CCCTGGGACG TCTCCCAGGG TTGCGGCCGG
            301 GTGTTCCGAA CTCGTCAGTT CCACCACGGG TCCGCCAGAT ACAGAGCTAG TTAGCTAACT
            361 AGTACCGACG CAGGCGCATA AAATCAGTCA TAGACACTAG ACAATCGGAC AGACACAGAT
            421 AAGTTGCTGG CCAGCTTACC TCCCGGTGGT GGGTCGGTGG TCCCTGGGCA GGGGTCTCCC
            481 GATCCCGGAC GAGCCCCCAA ATGAAAGACC CCCGCTGACG GGTAGTCAAT CACTCAGAGG
            541 AGACCCTCCC AAGGAACAGC GAGACCACAA GTCGGATGCA ACTGCAAGAG GGTTTATTGG
            601 ATACACGGGT ACCCGGGCGA CTCAGTCAAT CGGAGGACTG GCGCGCCGAG TGAGGGGTTG
            661 TGGGCTCTTT TATTGAGCTC GGGGAGCAGA AGCGCGCGAA CAGAAGCGAG AAGCGAACTG
            721 ATTGNTTAGT TCAAATAANG CACAGGGTCA TTTCAGGTCC TTGGGGCACC CTGGAAACAT
            781 CTGATGGNTC TCTAGAAACT GCTGANGGCT GGANCGCATC TGGGGANCAT CTGTTCNTGG
            841 NCNTGANCCG GGGCANGAAC TGCTTANCNC AGATATNCNG TTNGGCCNNT ANNCAGCNGT
          901 NNCATCTGNN NNNGGNCNNN NNCNNGGGNN NNANNNGNNT ANNNNNNNNN N
          //
          Primer: lacIpro-B_F (Pr0724)
          Region: 3' LTR
          Sequence file: RDB19010_B1Ece.seq check
          >D07716A7_B1Ec_8_lacIpro-B_F_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNGNN NCGNNNCNNT GNNCGNNGAA NCGGAANANC GCCCAATACG
             61 CAAACCGCCT CTCCCCGCGC GTTGGCCGAT TCATTAATGC AACTATGGCC ATTTAATGTA
            121 AATACTTAAG AAAAAAAACC AAATTAATTT TGATACATGC TGCATGTGAA AGACCCCCGC
            181 TGACGGGTAG TCAATCACTC AGAGGAGACC CTCCCAAGGA ACAGCGAGAC CACAAGTCGG
            241 ATGCAACTGC AAGAGGGTTT ATTGGATACA CGGGTACCCG GGCGACTCAG TCAATCGGAG
            301 GACTGGCGCC CCGAGTGAGG GGTTGTGGGC TCTTTTATTG AGCTCGGGGA GCAGAAGCGC
            361 GCGAACAGAA GCGAGAAGCG AACTGATTGG TTAGTTCAAA TAAGGCACAG GGTCATTTCA
            421 GGTCCTTGGG GCACCCTGGA AACATCTGAT GGTTCTCTAG AAACTGCTGA GGGCTGGACC
            481 GCATCTGGGG ACCATCTGTT CTTGGCCCTG AGCCGGGGCA GGAACTGCTT ACCACAGATA
            541 TCCTGTTTGG CCCATATTCA GCTGTTCCAT CTGTTCTTGG CCCTGAGCCG GGGCAGGAAC
            601 TGCTTACCAC AGATATCCTG TTTGGCCCAT ATTCAGCTGT TCCATCTGTT CCTGACCTTG
            661 ATCTGAACTT CTCTATTCTC AGTTATGTAT TTTTCNATGC CTTGCAAAAT GGCGTTACTT
            721 AAGCTAGCTT GCCAAACCTA CAGGTGGGGT CTTTCATTCC NCCCTTTTTC TGGAGACTAA
            781 ATAAAATCTT TTATTTTATC GTCGACCACT GTGCTGGCGG CCGCTTAAAA ATGCCTCTTC
            841 ATGTGTAANN NGAGGTGGTC NNANCTNNAA AATGNTCNGN CGCNTTTTTT NGCACTNGAA
            901 CGGGCGGNGC CCNNNGNGNT NANNNNANNG NCNNNNNNAN NNNNTCNNAN CGGNNGAANN
          961 TTNNNNNNNN NN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Borisova, E., Structurally-discovered KLF4 variants accelerate and stabilize reprogramming to pluripotency. iScience 25 (1): 103525 (2021). PMID 35106457. [link to RRC of NBRP]

          2023.08.29

          GNP_filter3_RDBDEP_html_230702.pl