Resource data sheet
DNA Bank Top

pBS-CaMKIIpro-G-CaMP9a-WPRE (#RDB19172)

Expression vector of genetically encoded calcium indicator, G-CaMP9a.


Drawn by SnapGene® software
Sequence (full) RDB19172hts01.seq
Publication Sakamoto, M., Cell Reports Methods 2: 100168 (2022). [link to RRC of NBRP]
Test sheet RDB19172_B4Djp1-1.pdf 
Assembled from experimentally sequenced data.
 
Clone info. Expression vector of genetically encoded calcium indicator, G-CaMP9a.
Comment Expression was confirmed by the depositor with transfection to cultured cells.
Vector backbone pBluescript II (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Camk2a genomic rat Calm2 cDNA; Aequorea victoria GFP (cpGFP) cDNA
Reference of insert sequence LC654935 (DDBJ accession number)
Depositor|Developer Bito, Haruhiko | Sakamoto, Masayuki | Inoue, Masatoshi |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sakamoto, M., et al., Cell Reports Methods 2: 100168 (2022)) is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Sakamoto, M., et al., Cell Reports Methods 2: 100168 (2022)) の引用を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19172 pBS-CaMKIIpro-G-CaMP9a-WPRE DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge


How to cite this biological resource

Materials & Methods section:

The pBS-CaMKIIpro-G-CaMP9a-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19172).

Reference section:

Sakamoto, M., Inoue, M., Takeuchi, A., Kobari, S., Yokoyama, T., Horigane, S., Takemoto-Kimura, S., Abe, M., Sakimura, K., Kano, M., Kitamura, K., Fujii, H., Bito, H., A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Reports Methods 2: 100168 (2022). PMID 35474964. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


References

Original, user report and related articles

original Sakamoto, M., A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Reports Methods 2: 100168 (2022). PMID 35474964. [link to RRC of NBRP]