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pBS-CaMKIIpro-G-CaMP9a-WPRE

Expression vector of genetically encoded calcium indicator, G-CaMP9a.

Catalog number RDB19172
Resource name pBS-CaMKIIpro-G-CaMP9a-WPRE
Clone info. Expression vector of genetically encoded calcium indicator, G-CaMP9a.
Comment Expression was confirmed by the depositor with transfection to cultured cells.
Vector backbone pBluescript II (plasmid)
Size of vector backbone 3.0 kb
Selectable markers Ampicillin
Gene/insert name mouse Camk2a genomic rat Calm2 cDNA
Reference of insert sequence LC654935 (DDBJ accession number)
Depositor|Developer Bito, Haruhiko | Sakamoto, Masayuki | Inoue, Masatoshi |
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External Database
mouse Camk2a rat Calm2

        Reference sequence
          
         

        Distribution information

        Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
        Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sakamoto, M., et al., Cell Reports Methods 2: 100168 (2022)) is requested.
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        提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Sakamoto, M., et al., Cell Reports Methods 2: 100168 (2022)) の引用を必要とする。
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        RDB19172 pBS-CaMKIIpro-G-CaMP9a-WPRE DNA solution

        Please review the QC test results indicated by check icon below as well as clone information before placing your order.

        How to cite this biological resource

        Materials & Methods section:

        The pBS-CaMKIIpro-G-CaMP9a-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19172).

        Reference section:

        Sakamoto, M., Inoue, M., Takeuchi, A., Kobari, S., Yokoyama, T., Horigane, S., Takemoto-Kimura, S., Abe, M., Sakimura, K., Kano, M., Kitamura, K., Fujii, H., Bito, H., A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Reports Methods 2: 100168 (2022). PMID 35474964.

        Further references such as user reports and related articles (go to bottom)


        References and tips

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        QC test results

        RIKEN BRC has sequenced portions of this material for quality test.
        Please review the QC test results indicated by check icon as well as clone information before placing your order.

        Test sheet RDB19172_B1Kup1-2.pdf check

        Nucleotide sequence of a portion of this resource (if available).

        Primer: pUC-M13_-100 (Pr0310)
        Region: T7 pro,insert 5'(mouse CaMKII pro)
        Sequence file: RDB19172_B1Kuf.seq check
        >D08506A1_B1Ku_1_pUC-M13_-100_B03_02_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG CCNGGGTTTT CCCNGTCACG ACGTTGTAAA
           61 ACGACGGCCA GTGAGCGCGC GTAATACGAC TCACTATAGG GCGAATTGGA GCTCCACCGC
          121 GGTGGCGGCC GCATGCATCA TTATGGCCTT AGGTCACTTC ATCTCCATGG GGTTCTTCTT
          181 CTGATTTTCT AGAAAATGAG ATGGGGGTGC AGAGAGCTTC CTCAGTGACC TGCCCAGGGT
          241 CACATCAGAA ATGTCAGAGC TAGAACTTGA ACTCAGATTA CTAATCTTAA ATTCCATGCC
          301 TTGGGGGCAT GCAAGTACGA TATACAGAAG GAGTGAACTC ATTAGGGCAG ATGACCAATG
          361 AGTTTAGGAA AGAAGAGTCC AGGGCAGGGT ACATCTACAC CACCCGCCCA GCCCTGGGTG
          421 AGTCCAGCCA CGTTCACCTC ATTATAGTTG CCTCTCTCCA GTCCTACCTT GACGGGAAGC
          481 ACAAGCAGAA ACTGGGACAG GAGCCCCAGG AGACCAAATC TTCATGGTCC CTCTGGGAGG
          541 ATGGGTGGGG AGAGCTGTGG CAGAGGCCTC AGGAGGGGCC CTGCTGCTCA GTGGTGACAG
          601 ATAGGGGTGA GAAAGCAGAC AGAGTCATTC CGTCAGCATT CTGGGTCTGT TTGGTACTTC
          661 TTCTCACGCT AAGGTGGCGG TGTGATATGC ACAATGGCTA AAAAGCAGGG AGAGCTGGAA
          721 AGAAACAAGG ACAGAGACAG AGGCCAAGTC AACCAGACCA ATTCCCAGAG GAAGCAAAGA
          781 AACCATTACA GANACTACNA GGGGGANNGG AAGGAGAGAT GANTTANCTT CCCCTGTAAA
          841 CCTTANAACC CANCTGTTGC NAGGGNNACG GGGCAATACC TGTCTCTTCA NANGANATGA
          901 AGTTGCCAGG GTANNTACNT CCTGTCTTNC TCANGGANCA TCCCNNAATG TNNNACCCAC
          961 TNNCCGTTAC CNNANCAACT GNCNTNTTTG CCCCNNTTNA NCCCATCCCG TCNNNNAAAN
        1021 NNNCCCTANN NNTNNNNNNN NGGGGNNNNN NNNNANNNNN NNNNN
        //
        Primer: SV40_F2 (Pr1194)
        Region: SV40 pA,lac pro,T3 pro
        Sequence file: RDB19172_B1Kug.seq check
        >D08506A1_B1Ku_1_SV40_F2_F03_06_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNTNNNGT CTTCGCCTTC GCCCTCNAGA NGAGTCGGAT
           61 CTCCCTTTGG GNCGNNTCCC CGCATCGGCC GGCCGCTTCG AGCAGACATG ATAAGATACA
          121 TTGATGAGTT TGGACAAACC ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA
          181 TTTGTGATGC TATTGCTTTA TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA
          241 ACAATTGCAT TCATTTTATG TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA
          301 AGTAAAACCT CTACAAATGT GGTAAAATCG ATAAGGATCC AACTCGAGCT ACTAGTAGGG
          361 TACCAGGAAT TCGTTTCGAA GCGTCGACTC TCCGGATTAA TTAAGTACCC AGCTTTTGTT
          421 CCCTTTAGTG AGGGTTAATT GCGCGCTTGG CGTAATCATG GTCATAGCTG TTTCCTGTGT
          481 GAAATTGTTA TCCGCTCACA ATTCCACACA ACATACGAGC CGGGAGCATA AAGTGTAAAG
          541 CCTGGGGTGC CTAATGAGTG AGCTAACTCA CATTAATTGC GTTGCGCTCA CTGCCCGCTT
          601 TCCAGTCGGG AAACCTGTCG TGCCAGCTGC ATTAATGAAT CGGCCAACGC GCGGGGAGAG
          661 GCGGTTTGCG TATTGGGCGC TCTTCCGCTT CCTCGCTCAC TGACTCGCTG CGCTCGGTCG
          721 TTCGGCTGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT
          781 CAGGGGATAA CGCAGGAAAG AACATGTGAG CNAAAAGGNC AGCAAAAGGC CAGGAACCGT
          841 AAAAANGNCG CGTTGCTGGC GTTTTNCCNT ANGCTCCGCC CCCCCTGANN ANCATCACAA
          901 AANTCGNNGC TCAAGTCANA NGTGGNGAAN NCCGNNNNNA NNNNNAAGNT ANCANGCGTT
        961 TCCCCNNNGA ANCTCCCTNN NGCNNTCTNC NGNTNCCNAN NNNNNNNNN
        //
        Primer: EGFP-C-rev (Pr0026)
        Region: insert mid(GCaMP9a 5',mouse CaMKII pro)
        Sequence file: RDB19172_B1Kuh.seq check
        >D08506A1_B1Ku_1_EGFP-C-rev_D03_04_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NGGNNTGGGT GCTCAGGTAG TGGTTGTCGG
           61 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTAG GCGAGCTGCA
          121 CGCCGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCGC CTTGATGCCG TTCTTCTGCT
          181 TGTCGGCCAT GATATAGACG TTCTCGAGAA ATGGGTTTCC AAAGGAACGC TTTCTCAGCA
          241 TAGACTTGAC CAGGATCACA GTGGTCCAGC TGGGGATGAG CTTGACGTCG ACCATGGTGG
          301 CGAGATCCTT ATCGTCATCG TCGTACAGAT CCCGACCCAT TTGCTGTCCA CCAGTCATGC
          361 TAGCCATACC ATGATGATGA TGATGATGAG AACCCATGGT GGCACCGGTA GCGCTGCCCC
          421 CAGAACTAGG GGCCACTCGC CTGCCCGTGC TCCTGAGTGC AAACGGAGAA CCGGCTTGAC
          481 TGACGAGCTT GGGGCTTCTG AGCAGGGCAC TGTGGCTGCT CACAGCCTCG CGAGCCTTCG
          541 TCAGCATCCA GGTCCCCATG GCAACCACCT CCGAAACGCC CTGTTCCCGT TGCCCCGGTA
          601 ACTGCCTCCC CAACACCTGC CTGCCTTTCA CTTTAAAACC TGCTCCTCTT TGCCCTGGCC
          661 TTATATACAC AATACTGCAG GACTGTGTGG GCCCAGGAGC AAGTGGACCC TGTTCCCCCA
          721 GGGTGATGTA CTGAGGGGGT TGGAGAAGGG GATGCGAACA AACTTAGTCC ACAAGTGATC
          781 ATCGCTCTTC CTACCTCCCC CACCTCNNAN CTATAGGGCC CTTTTNACAG ACNGGATGGG
          841 ATTAAGTGGG GCAAAGAGGC AGTTGNTATG GTAACGGCTA GTGGGTGCCA CNTTCTGGGN
          901 NGGNNCTTGA NAAANANAGG ANGTANTTAC CNTGNCANCN TCATCTCCNN TGAANANNCN
          961 NNNANTGCCC CNTTGCCCNN NNNNNNGCTG GNNNCTNNNN TTNCANGGGN NNNNNNANNN
        1021 NNNNNNN
        //
        Primer: PRE_rev (Pr0250)
        Region: insert mid(WPRE,GCaMP9a 3')
        Sequence file: RDB19172_B1Kui.seq check
        >D08506A1_B1Ku_1_PRE_rev_C03_03_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNGGAGCA ACATANNNNN NAATACCAGT CAATCTTTCA
           61 CAAATTTTGT AATCCANANG TTGATTATCT AGTACGCGTT CACTTCGCTG TCATCATTTG
          121 TACAAACTCT TCGTAGTTTA CCTGACCATC CCCATCGATG TCTGCTTCCC TGATCATTTC
          181 ATCAACCTCT TCATCTGTTA ACTTCTCTCC AAGGTTTGTC ATCACGTGGC GAAGCTCTGC
          241 TGCACTGATG TAGCCATTGC CATCCTTATC AAACACACGG AACGCTTCTC TAATTTCTTC
          301 TTCACTGTCT GTGTCTTTCA TTTTTCTTGC CATCATTGTC AGGAACTCAG GGAAGTCGAT
          361 TGTGCCATTA CCGTCGGCAT CTACTTCATT GATCATGTCC TGCAGCTCTG CTTCTGTGGG
          421 GTTCTGCCCC AGAGACCGCA GCACCGTCCC CAGCTCCTTG GTTGTTATTG TCCCATCCCC
          481 GTCCTTGTCA AATAGGGAGA AAGCCTCTTT AAATTCTGCG ATCTGCTCTT CAGTCAGTTG
          541 GTCCGTGTTG TACTCCAGCT TGTGCCCCAG GATGTTGCCG TCCTCCTTGA AGTCGATGCC
          601 CTTCAGCTCG ATGCGGTTCA CCAGGGTGTC GCCCTCGAAC TTCACCTCGG CGCGGGTCTT
          661 GTAGGTGCCG TCGTCCTTGA AGAAGATGGT GCGCTCCTGG ATGTAGCCTT CGGGCATGGC
          721 GGACTTGAAG AAGTCGTGCT GCTTCATGTG GTCGGGGTAG CGGCTGAAGC ACTGCACGCC
          781 GTANGTCANG GTGGTCACGA NGGTGGGCCA GGGCACGGGC AGCTTGCCNG TGGTGCAGAT
          841 GANCTTCNNG GTCAGCTTGC CGTTGGTGGC ATCGCCCTCA CCCTCGCCGC GCACGCTGAA
          901 CTTGTNNCCG TTNACGTCNC NNNCCAGCTC GANCAGGANG GGNACCACCC CNGNNANNAN
          961 CTNCNTCNNC CTTGCTCACN NTGNTCCNNN CNGNANNNCC CNTNGTANNN CNNGNCNATN
        1021 NNGANANTGA TNCNNNNNNN CNNNNANNNN NNNNNN
        //
        Primer: ECFP-C_F1 (Pr0925)
        Region: insert mid(GCaMP9a mid)
        Sequence file: RDB19172_B1Kuj.seq check
        >D08506A1_B1Ku_1_ECFP-C_F1_E03_05_ABI08.ab1
            1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN CGNNCNCNTN NNNCCTGCTG GGAGTTCGTG
           61 ACCGCCGCCG GGNTCACTCT CGGCATGGAC GAGCTGTACA AGGGCGGTAC CGGAGGGAGC
          121 ATGGTGAGCA AGGGCGAGGA GCTGTTCACC GGGGTGGTGC CCATCCTGGT CGAGCTGGAC
          181 GGCGACGTAA ACGGCCACAA GTTCAGCGTG CGCGGCGAGG GTGAGGGCGA TGCCACCAAC
          241 GGCAAGCTGA CCCTGAAGTT CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC
          301 CTCGTGACCA CCCTGACCTA CGGCGTGCAG TGCTTCAGCC GCTACCCCGA CCACATGAAG
          361 CAGCACGACT TCTTCAAGTC CGCCATGCCC GAAGGCTACA TCCAGGAGCG CACCATCTTC
          421 TTCAAGGACG ACGGCACCTA CAAGACCCGC GCCGAGGTGA AGTTCGAGGG CGACACCCTG
          481 GTGAACCGCA TCGAGCTGAA GGGCATCGAC TTCAAGGAGG ACGGCAACAT CCTGGGGCAC
          541 AAGCTGGAGT ACAACACGGA CCAACTGACT GAAGAGCAGA TCGCAGAATT TAAAGAGGCT
          601 TTCTCCCTAT TTGACAAGGA CGGGGATGGG ACAATAACAA CCAAGGAGCT GGGGACGGTG
          661 CTGCGGTCTC TGGGGCAGAA CCCCACAGAA GCAGAGCTGC AGGACATGAT CAATGAAGTA
          721 GATGCCGACG GTAATGGCAC AATCGACTTC CCTGAGTTCC TGACAATGAT GGCAAGAAAA
          781 ATGAAAGACA CAGACAGTGA AGAAGAAATT AGAGAAGCGT TCCGTGTGTT TGANANNGAT
          841 GGCAATGGCT ACNTCAGTGC ANCAGANCTT CNCCACGTGA TGACAANNCT TGGANAGAAN
          901 TTAACANATG AANANGTTGA NGAAANTGAT CAGGGANNCA NNNNTNNNTG GGGNNNGNNC
          961 AGGNNAANNA CNNNNNTTNN NANNAANNAT GNNNNCNAAN NGAANNCGTN NTNNNNNANN
        1021 NNNNNNNN
        //

        Please visit Sequencing and PCR primers for primer information.


        References

        Original, user report and related articles

        original Sakamoto, M., A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Reports Methods 2: 100168 (2022). PMID 35474964.

        2023.05.22

        GNP_filter3_RDBDEP_html_230512.pl