Expression vector of genetically encoded calcium indicator, G-CaMP9a.
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Clone info. | Expression vector of genetically encoded calcium indicator, G-CaMP9a. |
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Comment | Expression was confirmed by the depositor with transfection to cultured cells. |
Vector backbone | pBluescript II (plasmid) |
Size of vector backbone | 3.0 kb |
Selectable markers | Ampicillin |
Gene/insert name | mouse Camk2a genomic rat Calm2 cDNA; Aequorea victoria GFP (cpGFP) cDNA |
Reference of insert sequence | LC654935 (DDBJ accession number) |
Depositor|Developer | Bito, Haruhiko | Sakamoto, Masayuki | Inoue, Masatoshi | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment ![]() ![]() ![]() MTA, for use for not-for-profit academic purpose [Word ![]() ![]() Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR (Sakamoto, M., et al., Cell Reports Methods 2: 100168 (2022)) is requested. |
必要書類 | 提供依頼書 ![]() ![]() 提供同意書 (MTA, 非営利学術目的用)[Word ![]() ![]() 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献 (Sakamoto, M., et al., Cell Reports Methods 2: 100168 (2022)) の引用を必要とする。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB19172 | pBS-CaMKIIpro-G-CaMP9a-WPRE | DNA solution |
JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge |
Materials & Methods section:
The pBS-CaMKIIpro-G-CaMP9a-WPRE was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19172). |
Reference section:
Sakamoto, M., Inoue, M., Takeuchi, A., Kobari, S., Yokoyama, T., Horigane, S., Takemoto-Kimura, S., Abe, M., Sakimura, K., Kano, M., Kitamura, K., Fujii, H., Bito, H., A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Reports Methods 2: 100168 (2022). PMID 35474964. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
Original, user report and related articles
original | Sakamoto, M., A Flp-dependent G-CaMP9a transgenic mouse for neuronal imaging in vivo. Cell Reports Methods 2: 100168 (2022). PMID 35474964. [link to RRC of NBRP] |
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