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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAID1.2-EF1a-NmScarlet-mAID (#RDB19227)

Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously by EF1 alpha promoter for the super-sensitive AID system.

Clone info. Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously under the control of EF1 alpha promoter for the super-sensitive auxin-inducible degron (AID) system. See Fig.1 of Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020.
Vector backbone pBlueScript SK- (plasmid)
Selectable markers Ampicillin
Gene/insert name oriza sativa OsTIR1 cDNA
Depositor|Developer Fukagawa, Tatsuo |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB19227 pAID1.2-EF1a-NmScarlet-mAID DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAID1.2-EF1a-NmScarlet-mAID was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19227).

Reference section:

Nishimura, K., Yamada, R., Hagihara, S., Iwasaki, R., Uchida, N., Kamura, T., Takahashi, K., Torii, K.U., Fukagawa, T., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19227_B1H2p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F (Pr0168)
Region: EF1alpha pro,OsTIR1 5'
Sequence file: RDB19227_B1H2a.seq check
>D08293A2_B1H2_2_EF1a-C_F_A02_02_ABI24.ab1
    1 NNNNNNNNNN NNNCNTTTNT GANTTNGGNN CTTGGTTCNT TCTCNAGCCT CAGACAGTGG
   61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAATTGC GGAAGGCCGT CAAGGCCACG
  121 TGTCTTGTCC AGAGGCGCTG CCACCATGAC ATACTTTCCT GAAGAGGTCG TCGAACACAT
  181 TTTTAGCTTC CTGCCTGCAC AGAGAGATAG AAACACAGTG AGCCTGGTCT GCAAAGTGTG
  241 GTACGAGATC GAACGCCTGA GCCGGAGAGG AGTGTTCGTC GGCAACTGCT ATGCTGTGAG
  301 AGCAGGCAGG GTCGCCGCTA GGTTTCCAAA TGTGCGCGCA CTGACCGTCA AGGGGAAACC
  361 CCACTTCGCC GACTTTAACC TGGTGCCCCC TGATTGGGGA GGATACGCCG GCCCTTGGAT
  421 CGAGGCAGCC GCTCGCGGCT GTCATGGACT GGAGGAACTG CGCATGAAGC GAATGGTGGT
  481 CTCTGACGAA AGTCTGGAGC TGCTGGCTCG GAGCTTCCCT AGGTTTCGCG CACTGGTGCT
  541 GATTTCTTGC GAAGGCTTCA GCACCGATGG ACTGGCAGCC GTGGCCTCCC ACTGTAAGCT
  601 GCTGCGGGAG CTGGACCTCC AGGAGAATGA AGTGGAGGAT AGAGGCCCCA GATGGCTGTC
  661 TTGCTTCCCA GACTCATGTA CCAGCCTGGT GTCCCTGAAC TTTGCCTGCA TCAAAGGCGA
  721 AGTGAATGCT GGGTCCCTGG AGCGGCTGGT CTCAAGAAGC CCCAACCTGA NGTCTCTGCG
  781 GCTGANCCGG ANCGTGAGCG TGGACACTCT NGCTAAGANT CTGCTGAGAN CCCCTAACCT
  841 GGNNGANCTG GNAANCGGCA ATCTGACAGA NGANTTCCAG ACNGAATCCT ACTTTAAACT
  901 GACTTCTGNC CTGGANAANT GTAAAANGCT GANGANTCTG TCNNNANTCN GGGNNGCTTN
961 NCCCGNGNGC NNNANCTTTA TCTANCNNNN NNNNGNACAG NNNNNNNNNN
//
Primer: SV40polyA_R2 (Pr0623)
Region: mAID,mScarlet 3'
Sequence file: RDB19227_B1H2b.seq check
>D08293A2_B1H2_2_SV40polyA_R2_D02_11_ABI24.ab1
    1 NNNNNNNNNN NNNNNNGNAN NNNNAGTTAA NNANNANNAT TGGANATCGG CACCCGCTCC
   61 AGCGCCTGCA CCAGCTCCTT TATACATCCT CAAATCGATT TTCCTCAAGT ACGGTGCTCC
  121 GTCCATTGAT ACCTTCACGA ACGCCGCCGC CTCCGGGCCA CCGCTTGATT TTTGGCAGGA
  181 AACCATCACG TTCTTCCGGT ATGATCTCAC CGGTGGCCAT CCCACAACTT GTGCCTTGGC
  241 CGGAGGTTTG GCTGGATCTT TAGGACAAGC ACTCTTCTCC TTCTTGTACA GCTCGTCCAT
  301 GCCGCCGGTG GAGTGGCGGC CCTCGGAGCG TTCGTACTGT TCCACCACGG TGTAGTCCTC
  361 GTTGTGGGAG GTGATGTCCA ACTTGCGGTC GACGTTGTAG GCGCCGGGCA TCTGCACGGG
  421 CTTCTTGGCC TTGTAGGTGG TCTTGAAGTC CGCCAGGTAG CGGCCGCCGT CCTTCAGGCG
  481 CAGGGCCATC TTAATGTCGC CCTTCAGCAC GCCGTCCTCG GGGTACAACC GCTCGGTGGA
  541 CGCTTCCCAG CCCATTGTCT TCTTCTGCAT TACGGGGCCG TCAGGAGGGA AGTTGGTGCC
  601 GCGGAGCTTC ACCTTGTAGA TCAGGGTGCC GTCCTCCAGG GAGGTGTCCT GGGTCACGGT
  661 CACGGCGCCG CCGTCCTCGA AGTTCATCAC GCGCTCCCAC TTGAAGCCCT CGGGGAAGGA
  721 CTGCTTATAG TAGTCGGGGA TGTCGGCGGG GTGCTTGATG AAGGNCCTGG ANCCGTACAT
  781 GAACTGANGG GACAGGATGT CCCAGGANAA NGGCANGGGG CCACCCTTGG TCACCTTCAG
  841 CTTGGCGGTC TGNNTGCCCN CGTANGGGCG GNCCTCGCCC NCGCCCTCGA TCTCGAACTC
  901 GNGGCCGTNC ATGGANCCCN NCATGTGCNN CTNNNNCCGC ATGAACTCCT TGATCACTGN
  961 CNCNCCCTTG CNCACCANNN NATCGCACAT NGNNNNNGGN NNNANNANNA TCGNNNTTTN
1021 NNNNNANNNN NNNNNCNNNN NNNNNN
//
Primer: IRES-R (Pr0037)
Region: bsr,T2A,OsTIR1 3'
Sequence file: RDB19227_B1H2c.seq check
>D08293A2_B1H2_2_IRES-R_B02_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGGGGGGG GNNGGNNAGG GGCGGANCCC
   61 TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG GAAAAAGATC CTTAATTTCG
  121 GGTATATTTG AGTGGAATGA GTTCTTCAAT CGTAGTTTTG ACTAACTTGC CATTCATTTC
  181 TATTAACACA AAACAATCTG GTGCATAGTC TGAAATCAAC TCCCTACACA TACCACAAGG
  241 ACTTACCACT CGAATACTTC TATCTACTTC GTCAGAATAA GGGTGTCTAA CAGCTACAAT
  301 CGTGTCAAAA TCCTTTTGTC CATTCGAAAC TGCACTACCA ATCGCAATGG CTTCTGCACA
  361 AACAGTTACT CGTCCTATAT ACGCTTCAAT ATGTACTGCC GAAATGATTT CTCCTGTTTT
  421 CGTACGAATT GCCGCTCCCA CATGATGTTT ATTATCCTCA TAAAGCATTG TAATCTTCTC
  481 TGTCGCTACT TCTACTAATT CTAGATCTTG TTGAGAAATG TTAAATGTTT TCATGGGGCC
  541 GGGGTTCTCC TCCACGTCGC CGCAGGTCAG CAGGCTGCCG CGGCCCTCCA GAATCTTCAC
  601 AAAGTTGGGA GCATCATCTC TTGCCCCTGC GGTGGTCCGA TACACATACA GTTTCTCCAC
  661 CTTTGGCAGG TCGCCATGAT TTTCCTCCAT TTCGTTAGAC CCGTCCCGCT CATTAATGAC
  721 TTCCACGTTC AGCATAGGCA TTTTACTAGC CAGGACCTGA CAGCCCTTCA GAGTCACATT
  781 GCAACTAGAC ATCCACAGGC TCCTCATTGT CTCGTATCTG GCAAAGTTTC CNAGCAGANC
  841 GGCATCCCCG AAAGGGCTGT CCCGGANTNC CAGTTTTCGC ANATTCTTAC NCCCGTTCAT
  901 CACGTGCATC ANGNCCTTAT CGNANNCTCC GGCAAATGNN ANGCTCANCA TCTCCAGCTG
  961 CTNANCANAC TTGNCGANGT NNNTGNNNNN TTTNNNNGTC ANCNNNNNNN AAATGNTCAN
1021 NNNNNNGNNN NCCNNNNNNN NNN
//
Primer: IRES-F (Pr0036)
Region: IRES2,mScarlet 5'
Sequence file: RDB19227_B1H2d.seq check
>D08293A2_B1H2_2_IRES-F_C02_08_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNTNN GNCGAGGTTN AANAAACGTC TAGGCCCCCC GAACCNCGGG
   61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGT GCGATAACAT
  121 GGTGAGCAAG GGCGAGGCAG TGATCAAGGA GTTCATGCGG TTCAAGGTGC ACATGGAGGG
  181 CTCCATGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG
  241 CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCT CCTGGGACAT
  301 CCTGTCCCCT CAGTTCATGT ACGGCTCCAG GGCCTTCATC AAGCACCCCG CCGACATCCC
  361 CGACTACTAT AAGCAGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA
  421 GGACGGCGGC GCCGTGACCG TGACCCAGGA CACCTCCCTG GAGGACGGCA CCCTGATCTA
  481 CAAGGTGAAG CTCCGCGGCA CCAACTTCCC TCCTGACGGC CCCGTAATGC AGAAGAAGAC
  541 AATGGGCTGG GAAGCGTCCA CCGAGCGGTT GTACCCCGAG GACGGCGTGC TGAAGGGCGA
  601 CATTAAGATG GCCCTGCGCC TGAAGGACGG CGGCCGCTAC CTGGCGGACT TCAAGACCAC
  661 CTACAAGGCC AAGAAGCCCG TGCAGATGCC CGGCGCCTAC AACGTCGACC GCAAGTTGGA
  721 CATCACCTCC CACAACGAGG ACTACACCGT GGTGGAACAG TACGAACGCT CCGANGGNCG
  781 CCACTCCACC GGCGGCATGG ACGANCTGTA CAAGAAGGAN AAGANTGCTT GTCCTAAAGA
  841 TCCAGCCAAA CCTCCGGCCN AGGCACNNGT TGTGGGANGG CCNNCGGNGA NNNCATACCN
  901 GAAGANNGTG ATGGTTNCCT GNNNAAAATC AANCGGNGGC CCGGANNNGG NGGNGTNCNN
  961 GNANGNNATC NNNGGANNNA NCNNNNNACT NNANNAAAAT NNNTTTGANN NNGNNNNANN
1021 GNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]