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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pAID1.2-EF1a-NmScarlet-mAID

Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously by EF1 alpha promoter for the super-sensitive AID system.

Catalog number RDB19227
Resource name pAID1.2-EF1a-NmScarlet-mAID
Clone info. Expresson vector to construct mScarlet-mAID tagged target gene and OsTIR1 (WT) simultaneously under the control of EF1 alpha promoter for the super-sensitive auxin-inducible degron (AID) system. See Fig.1 of Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020.
Vector backbone pBlueScript SK- (plasmid)
Selectable markers Ampicillin
Gene/insert name oriza sativa OsTIR1 cDNA
Depositor|Developer Fukagawa, Tatsuo |
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External Database
oriza sativa OsTIR1

          Reference sequence
            
           

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR is requested (Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Nishimura, K. et al. Nucleic Acids Res. 48 (18): e108, 2020)。
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          RDB19227 pAID1.2-EF1a-NmScarlet-mAID DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pAID1.2-EF1a-NmScarlet-mAID was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19227).

          Reference section:

          Nishimura, K., Yamada, R., Hagihara, S., Iwasaki, R., Uchida, N., Kamura, T., Takahashi, K., Torii, K.U., Fukagawa, T., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]

          Further references such as user reports and related articles (go to bottom)


          Tips

          Featured content

          Featured content Auxin Inducible Degron (AID) System (English text)
          Featured content Auxin Inducible Degron (AID) System (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB19227_B1H2p1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F (Pr0168)
          Region: EF1alpha pro,OsTIR1 5'
          Sequence file: RDB19227_B1H2a.seq check
          >D08293A2_B1H2_2_EF1a-C_F_A02_02_ABI24.ab1
              1 NNNNNNNNNN NNNCNTTTNT GANTTNGGNN CTTGGTTCNT TCTCNAGCCT CAGACAGTGG
             61 TTCAAAGTTT TTTTCTTCCA TTTCAGGTGT CGTGAATTGC GGAAGGCCGT CAAGGCCACG
            121 TGTCTTGTCC AGAGGCGCTG CCACCATGAC ATACTTTCCT GAAGAGGTCG TCGAACACAT
            181 TTTTAGCTTC CTGCCTGCAC AGAGAGATAG AAACACAGTG AGCCTGGTCT GCAAAGTGTG
            241 GTACGAGATC GAACGCCTGA GCCGGAGAGG AGTGTTCGTC GGCAACTGCT ATGCTGTGAG
            301 AGCAGGCAGG GTCGCCGCTA GGTTTCCAAA TGTGCGCGCA CTGACCGTCA AGGGGAAACC
            361 CCACTTCGCC GACTTTAACC TGGTGCCCCC TGATTGGGGA GGATACGCCG GCCCTTGGAT
            421 CGAGGCAGCC GCTCGCGGCT GTCATGGACT GGAGGAACTG CGCATGAAGC GAATGGTGGT
            481 CTCTGACGAA AGTCTGGAGC TGCTGGCTCG GAGCTTCCCT AGGTTTCGCG CACTGGTGCT
            541 GATTTCTTGC GAAGGCTTCA GCACCGATGG ACTGGCAGCC GTGGCCTCCC ACTGTAAGCT
            601 GCTGCGGGAG CTGGACCTCC AGGAGAATGA AGTGGAGGAT AGAGGCCCCA GATGGCTGTC
            661 TTGCTTCCCA GACTCATGTA CCAGCCTGGT GTCCCTGAAC TTTGCCTGCA TCAAAGGCGA
            721 AGTGAATGCT GGGTCCCTGG AGCGGCTGGT CTCAAGAAGC CCCAACCTGA NGTCTCTGCG
            781 GCTGANCCGG ANCGTGAGCG TGGACACTCT NGCTAAGANT CTGCTGAGAN CCCCTAACCT
            841 GGNNGANCTG GNAANCGGCA ATCTGACAGA NGANTTCCAG ACNGAATCCT ACTTTAAACT
            901 GACTTCTGNC CTGGANAANT GTAAAANGCT GANGANTCTG TCNNNANTCN GGGNNGCTTN
          961 NCCCGNGNGC NNNANCTTTA TCTANCNNNN NNNNGNACAG NNNNNNNNNN
          //
          Primer: SV40polyA_R2 (Pr0623)
          Region: mAID,mScarlet 3'
          Sequence file: RDB19227_B1H2b.seq check
          >D08293A2_B1H2_2_SV40polyA_R2_D02_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNGNAN NNNNAGTTAA NNANNANNAT TGGANATCGG CACCCGCTCC
             61 AGCGCCTGCA CCAGCTCCTT TATACATCCT CAAATCGATT TTCCTCAAGT ACGGTGCTCC
            121 GTCCATTGAT ACCTTCACGA ACGCCGCCGC CTCCGGGCCA CCGCTTGATT TTTGGCAGGA
            181 AACCATCACG TTCTTCCGGT ATGATCTCAC CGGTGGCCAT CCCACAACTT GTGCCTTGGC
            241 CGGAGGTTTG GCTGGATCTT TAGGACAAGC ACTCTTCTCC TTCTTGTACA GCTCGTCCAT
            301 GCCGCCGGTG GAGTGGCGGC CCTCGGAGCG TTCGTACTGT TCCACCACGG TGTAGTCCTC
            361 GTTGTGGGAG GTGATGTCCA ACTTGCGGTC GACGTTGTAG GCGCCGGGCA TCTGCACGGG
            421 CTTCTTGGCC TTGTAGGTGG TCTTGAAGTC CGCCAGGTAG CGGCCGCCGT CCTTCAGGCG
            481 CAGGGCCATC TTAATGTCGC CCTTCAGCAC GCCGTCCTCG GGGTACAACC GCTCGGTGGA
            541 CGCTTCCCAG CCCATTGTCT TCTTCTGCAT TACGGGGCCG TCAGGAGGGA AGTTGGTGCC
            601 GCGGAGCTTC ACCTTGTAGA TCAGGGTGCC GTCCTCCAGG GAGGTGTCCT GGGTCACGGT
            661 CACGGCGCCG CCGTCCTCGA AGTTCATCAC GCGCTCCCAC TTGAAGCCCT CGGGGAAGGA
            721 CTGCTTATAG TAGTCGGGGA TGTCGGCGGG GTGCTTGATG AAGGNCCTGG ANCCGTACAT
            781 GAACTGANGG GACAGGATGT CCCAGGANAA NGGCANGGGG CCACCCTTGG TCACCTTCAG
            841 CTTGGCGGTC TGNNTGCCCN CGTANGGGCG GNCCTCGCCC NCGCCCTCGA TCTCGAACTC
            901 GNGGCCGTNC ATGGANCCCN NCATGTGCNN CTNNNNCCGC ATGAACTCCT TGATCACTGN
            961 CNCNCCCTTG CNCACCANNN NATCGCACAT NGNNNNNGGN NNNANNANNA TCGNNNTTTN
          1021 NNNNNANNNN NNNNNCNNNN NNNNNN
          //
          Primer: IRES-R (Pr0037)
          Region: bsr,T2A,OsTIR1 3'
          Sequence file: RDB19227_B1H2c.seq check
          >D08293A2_B1H2_2_IRES-R_B02_05_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNGGGGGGGG GNNGGNNAGG GGCGGANCCC
             61 TCAAGGGGCT TCATGATGTC CCCATAATTT TTGGCAGAGG GAAAAAGATC CTTAATTTCG
            121 GGTATATTTG AGTGGAATGA GTTCTTCAAT CGTAGTTTTG ACTAACTTGC CATTCATTTC
            181 TATTAACACA AAACAATCTG GTGCATAGTC TGAAATCAAC TCCCTACACA TACCACAAGG
            241 ACTTACCACT CGAATACTTC TATCTACTTC GTCAGAATAA GGGTGTCTAA CAGCTACAAT
            301 CGTGTCAAAA TCCTTTTGTC CATTCGAAAC TGCACTACCA ATCGCAATGG CTTCTGCACA
            361 AACAGTTACT CGTCCTATAT ACGCTTCAAT ATGTACTGCC GAAATGATTT CTCCTGTTTT
            421 CGTACGAATT GCCGCTCCCA CATGATGTTT ATTATCCTCA TAAAGCATTG TAATCTTCTC
            481 TGTCGCTACT TCTACTAATT CTAGATCTTG TTGAGAAATG TTAAATGTTT TCATGGGGCC
            541 GGGGTTCTCC TCCACGTCGC CGCAGGTCAG CAGGCTGCCG CGGCCCTCCA GAATCTTCAC
            601 AAAGTTGGGA GCATCATCTC TTGCCCCTGC GGTGGTCCGA TACACATACA GTTTCTCCAC
            661 CTTTGGCAGG TCGCCATGAT TTTCCTCCAT TTCGTTAGAC CCGTCCCGCT CATTAATGAC
            721 TTCCACGTTC AGCATAGGCA TTTTACTAGC CAGGACCTGA CAGCCCTTCA GAGTCACATT
            781 GCAACTAGAC ATCCACAGGC TCCTCATTGT CTCGTATCTG GCAAAGTTTC CNAGCAGANC
            841 GGCATCCCCG AAAGGGCTGT CCCGGANTNC CAGTTTTCGC ANATTCTTAC NCCCGTTCAT
            901 CACGTGCATC ANGNCCTTAT CGNANNCTCC GGCAAATGNN ANGCTCANCA TCTCCAGCTG
            961 CTNANCANAC TTGNCGANGT NNNTGNNNNN TTTNNNNGTC ANCNNNNNNN AAATGNTCAN
          1021 NNNNNNGNNN NCCNNNNNNN NNN
          //
          Primer: IRES-F (Pr0036)
          Region: IRES2,mScarlet 5'
          Sequence file: RDB19227_B1H2d.seq check
          >D08293A2_B1H2_2_IRES-F_C02_08_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTNN GNCGAGGTTN AANAAACGTC TAGGCCCCCC GAACCNCGGG
             61 GACGTGGTTT TCCTTTGAAA AACACGATGA TAATATGGCC ACAACCATGT GCGATAACAT
            121 GGTGAGCAAG GGCGAGGCAG TGATCAAGGA GTTCATGCGG TTCAAGGTGC ACATGGAGGG
            181 CTCCATGAAC GGCCACGAGT TCGAGATCGA GGGCGAGGGC GAGGGCCGCC CCTACGAGGG
            241 CACCCAGACC GCCAAGCTGA AGGTGACCAA GGGTGGCCCC CTGCCCTTCT CCTGGGACAT
            301 CCTGTCCCCT CAGTTCATGT ACGGCTCCAG GGCCTTCATC AAGCACCCCG CCGACATCCC
            361 CGACTACTAT AAGCAGTCCT TCCCCGAGGG CTTCAAGTGG GAGCGCGTGA TGAACTTCGA
            421 GGACGGCGGC GCCGTGACCG TGACCCAGGA CACCTCCCTG GAGGACGGCA CCCTGATCTA
            481 CAAGGTGAAG CTCCGCGGCA CCAACTTCCC TCCTGACGGC CCCGTAATGC AGAAGAAGAC
            541 AATGGGCTGG GAAGCGTCCA CCGAGCGGTT GTACCCCGAG GACGGCGTGC TGAAGGGCGA
            601 CATTAAGATG GCCCTGCGCC TGAAGGACGG CGGCCGCTAC CTGGCGGACT TCAAGACCAC
            661 CTACAAGGCC AAGAAGCCCG TGCAGATGCC CGGCGCCTAC AACGTCGACC GCAAGTTGGA
            721 CATCACCTCC CACAACGAGG ACTACACCGT GGTGGAACAG TACGAACGCT CCGANGGNCG
            781 CCACTCCACC GGCGGCATGG ACGANCTGTA CAAGAAGGAN AAGANTGCTT GTCCTAAAGA
            841 TCCAGCCAAA CCTCCGGCCN AGGCACNNGT TGTGGGANGG CCNNCGGNGA NNNCATACCN
            901 GAAGANNGTG ATGGTTNCCT GNNNAAAATC AANCGGNGGC CCGGANNNGG NGGNGTNCNN
            961 GNANGNNATC NNNGGANNNA NCNNNNNACT NNANNAAAAT NNNTTTGANN NNGNNNNANN
          1021 GNNNNNNNNN N
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nishimura, K., A super-sensitive auxin-inducible degron system with an engineered auxin-TIR1 pair. Nucleic Acids Res. 48 (18): e108 (2020). PMID 32941625. [link to RRC of NBRP]

          2024.03.26

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