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pFN21A_Halo_CK1alpha_K46R

Expresion vector of human CK1 alpha kinase-dead mutant (K46R) tagged with Halo at N-terminus.

Catalog number RDB19304
Resource name pFN21A_Halo_CK1alpha_K46R
Alternative name pFN21A_Halo_CK1α_K46R
Clone info. Expresion vector of human casein kinase 1 alpha 1 (CK1 alpha) kinase-dead mutant (K46R) tagged with Halo at N-terminus. See Fig.6 of Kinoshita-Kikuta, E. et al. Sci. Rep. 10 (1): 16273, 2020.
Comment Expression was confirmed by the depositor with SDS-PAGE, immunoblotting.
Vector backbone pFN21A HaloTag CMV Flexi (plasmid)
Size of vector backbone 5.1 kb
Selectable markers Ampicillin
Gene/insert name human CSNK1A1 cDNA
Depositor|Developer Kinoshita, Emiko |
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External Database
human CSNK1A1

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          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Kinoshita-Kikuta, E. et al., Sci. Rep. 10 (1): 16273, 2020)
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Kinoshita-Kikuta, E. et al., Sci. Rep. 10 (1): 16273, 2020)。
          付加的提供条件 本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。
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          RDB19304 pFN21A_Halo_CK1alpha_K46R Under QC test. Please contact us. DNA solution

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.

          References and tips

          Electronic file

          Original reference

          original Kinoshita-Kikuta, E., Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep. 10 (1): 16273 (2020). PMID 33004926.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          check Please wait for results of QC test to be uploaded. This clone will be sequenced a portion for examination.


          References

          Original, user report and related articles

          original Kinoshita-Kikuta, E., Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep. 10 (1): 16273 (2020). PMID 33004926.

          2022.05.16

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