Please review the QC test results indicated by icon below as well as clone information before placing your order.

Target BFP gene expression plasmid

Expression vector of target BFP gene, a point mutant (T199C) of GFP for artificial enzymatic RNA editing system.

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Bhakta, S. et al., Sci. Rep. 10 (1): 17304, 2020)
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提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Bhakta, S. et al., Sci. Rep. 10 (1): 17304, 2020)。
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Please review the QC test results indicated by icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

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Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19359_B1Jqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016) Region: CMV pro,T7 pro,insert 5' Sequence file: RDB19359_B1Jqa.seq
>19359_19359_B1Jg_2_CMV-Forward_B03.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN TGGGCGGTAG GCGNNNNNNG NGGGAGGTCT    61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA   121 ATACGACTCA CTATAGGGAG ACCCAAGCTT CCGGCGGTTC TGACATCCGG CGGTTCTGAC   181 ATCCGGCGGT TCTGACATCC GGCGGTTCTG ACATCCGGCG GTTCTGACAT AAGCTTGGTA   241 CCGAGCTCGG ATCCCACCAT GGACTACAAA GACGATGACG ATAAAGGGGG CGGTGGAGGT   301 TACCCATACG ATGTTCCAGA TTACGCTGGT GGAGGTGGAG GTGAATTCAT GGTGAGCAAG   361 GGCGAGGAGC TGTTCACCGG GGTGGTGCCC ATCCTGGTCG AGCTGGACGG CGACGTAAAC   421 GGCCACAAGT TCAGCGTGTC CGGCGAGGGC GAGGGCGATG CCACCTACGG CAAGCTGACC   481 CTGAAGTTCA TCTGCACCAC CGGCAAGCTG CCCGTGCCCT GGCCCACCCT CGTGACCACC   541 CTGTCCCACG GCGTGCAGTG CTTCAGCCGC TACCCCGACC ACATGAAGCA GCACGACTTC   601 TTCAAGTCCG CCATGCCCGA AGGCTACGTC CAGGAGCGCA CCATCTTCTT CAAGGACGAC   661 GGCAACTACA AGACCCGCGC CGAGGTGAAG TTCGAGGGCG ACACCCTGGT GAACCGCATC   721 GAGCTGAAGG GCATCGACTT CAAGGAGGAC GGCAACATCC TGGGGCACAA GCTGGAGTAC   781 AACTTCNACA GCCACAACGT CTATATCATG GCCGACAAGC ANAAGAACGG CATCNAGGCG   841 AANTTCAAGA TCCGCCACAA CATCGAGGAN GGCAGCGTGC AGCTCGCCNA NNNCTACNNN   901 CAGAACACCC CCNTCGGCGA NNGNCCCNNG CTGNTGCCCG ACAACCACTA NCTGANCNNC   961 CNNTNCGCCC TGANCAAGAN CCCANNGANN ANNGCGATNN CNTGGNCCTG CTGGNNTTCG 1021 TGNNCNNCNC CNGGNTNNCT CNCGGCANGN NNNNNCNNNN NNNNTNNNAN NNNNNNNNN //
Primer: bGH_rev3 Sequence file: RDB19359_B1Jqb.seq
>19359_19359_B1Jg_2_bGH_rev3_C03.ab1     1 NNNNNNNNNN NNNGNNGNCT GGCAACTAGA AGGCACAGTC GAGGCTGATC AGCGAGCTCT    61 AGCATTTAGG TGACACTATA GAATAGGGCC CTCTAGATGC ATGCTCGAGC GGCCGCCAGT   121 GTGATGGATA TCTTACTTGT ACAGCTCGTC CATGCCGAGA GTGATCCCGG CGGCGGTCAC   181 GAACTCCAGC AGGACCATGT GATCGCGCTT CTCGTTGGGG TCTTTGCTCA GGGCGGACTG   241 GGTGCTCAGG TAGTGGTTGT CGGGCAGCAG CACGGGGCCG TCGCCGATGG GGGTGTTCTG   301 CTGGTAGTGG TCGGCGAGCT GCACGCTGCC GTCCTCGATG TTGTGGCGGA TCTTGAAGTT   361 CGCCTTGATG CCGTTCTTCT GCTTGTCGGC CATGATATAG ACGTTGTGGC TGTTGAAGTT   421 GTACTCCAGC TTGTGCCCCA GGATGTTGCC GTCCTCCTTG AAGTCGATGC CCTTCAGCTC   481 GATGCGGTTC ACCAGGGTGT CGCCCTCGAA CTTCACCTCG GCGCGGGTCT TGTAGTTGCC   541 GTCGTCCTTG AAGAAGATGG TGCGCTCCTG GACGTAGCCT TCGGGCATGG CGGACTTGAA   601 GAAGTCGTGC TGCTTCATGT GGTCGGGGTA GCGGCTGAAG CACTGCACGC CGTGGGACAG   661 GGTGGTCACG AGGGTGGGCC AGGGCACGGG CAGCTTGCCG GTGGTGCAGA TGAACTTCAG   721 GGTCAGCTTG CCGTANGTGG CATCGCCCTC GCCCTCGCCG GACACGCTGA ACTTGTGGCC   781 GTTTACGTCG CCGTCCAGCT CGACCAGGAT GGGCACCACC CCGGTGAACA GCTCCTCGCC   841 CTTGCTCACC ATGAATTCNC CTCCNCCTNC ACCAGCGTAA TCTGGAANAT CGNATGGGTA   901 ACCNCCNCNG CCCCCTTTAT CGNCNTCGNC TTTGTANNCC ANGGNGGGNN NNGAGCTCNG   961 TACCNNNCTT ANGTCANANN CNNNNNGNNG NCNNANNNNN CNNGNNNNNN NANNCNNNNN 1021 GNANNNNNNN NNN //
Primer: SV40pro_ori_F Sequence file: RDB19359_B1Jqc.seq
>19359_19359_B1Jg_2_SV40pro_ori_F_D03.ab1     1 NNNNNNNNNN CNNCTGCCTC TGAGCTATTC NNGAAGTAGT GAGGAGGCTT TTTTGGAGGC    61 CTAGGCTTTT GCAAAAAGCT CCCGGGAGCT TGTATATCCA TTTTCGGATC TGATCAAGAG   121 ACAGGATGAG GATCGTTTCG CATGATTGAA CAAGATGGAT TGCACGCAGG TTCTCCGGCC   181 GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC AGACAATCGG CTGCTCTGAT   241 GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC TTTTTGTCAA GACCGACCTG   301 TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC TATCGTGGCT GGCCACGACG   361 GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG CGGGAAGGGA CTGGCTGCTA   421 TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC TTGCTCCTGC CGAGAAAGTA   481 TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG ATCCGGCTAC CTGCCCATTC   541 GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC GGATGGAAGC CGGTCTTGTC   601 GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC CAGCCGAACT GTTCGCCAGG   661 CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA CCCATGGCGA TGCCTGCTTG   721 CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA TCGACTGTGG CCGGCTGGGT   781 GTGGCGGANC GCTATCANGN CATAGCGTTG GCTACNCGTG ATATTGCTGA AGANCTTGGC   841 GGCGAATGGG CTGANCGCTT CCTCGTGCTT TACGGTATCG CCNCTCCCGA TTCGCANCGC   901 ATCGCCNTCT ATCGCCTTCN NGANNANTNC NNCTGANCNG GANTCTGGGG TTCNAAATGA   961 NCGANCNAGC NNCNCCCNNN CNNGNCNNCN NNNNATTTCN ATNNCNCCNN NNNCNNNNNN 1021 NNNAANNNTN NGNNNNNNNN NN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.01

GNP_filter3_RDBDEP_html_230501.pl