RDB19438 - pET-G4Null

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icon below as well as clone information before placing your order.

pET-G4Null (#RDB19438)

Bacterial expression vector of human galectin-4 with a partially truncated linker peptide (tag-free protein).

Clone info.	Bacterial expression vector of human galectin-4 with a partially truncated linker peptide (tag-free protein).
Comment	Expression was confirmed by the depositor with SDS-PAGE and Western blotting.
Vector backbone	pET-11a (plasmid)
Size of vector backbone	5.7 kb
Selectable markers	Ampicillin
Gene/insert name	human LGALS4 cDNA
Depositor\|Developer	Nishi, Nozomu \|

Sequence (full)	RDB19438zzt01.seq compiled by us

Distribution information

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the biological resource is limited to a recipient of a not-for-profit organization for a not-for-profit research.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	本件リソースの利用は、学術機関の学術研究に限る。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB19438	pET-G4Null	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pET-G4Null was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19438).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB19438_B1Kqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pET-B_F (Pr0723) Region: T7 pro, lacO, RBS, insert 5' Sequence file: RDB19438_B1Kqa.seq
>D08453A5_B1Kq_2_pET-B_F_G01_19_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGNCGGCN NCGATGCGTC CGGCGTANAG GANCGAGATC 61 TCGATCCCGC GAAATTAATA CGACTCACTA TAGGGGAATT GTGAGCGGAT AACAATTCCC 121 CTCTAGAAAT AATTTTGTTT AACTTTAAGA AGGAGATATA CATATGGCCT ATGTCCCCGC 181 ACCGGGCTAC CAGCCCACCT ACAACCCGAC GCTGCCTTAC TACCAGCCCA TCCCGGGCGG 241 GCTCAACGTG GGAATGTCTG TTTACATCCA AGGAGTGGCC AGCGAGCACA TGAAGCGGTT 301 CTTCGTGAAC TTTGTGGTTG GGCAGGATCC GGGCTCAGAC GTCGCCTTCC ACTTCAATCC 361 GCGGTTTGAC GGCTGGGACA AGGTGGTCTT CAACACGTTG CAGGGCGGGA AGTGGGGCAG 421 CGAGGAGAGG AAGAGGAGCA TGCCCTTCAA AAAGGGTGCC GCCTTTGAGC TGGTCTTCAT 481 AGTCCTGGCT GAGCACTACA AGGTGGTGGT AAATGGAAAT CCCTTCTATG AGTACGGGCA 541 CCGGCTTCCC CTACAGATGG TCACCCACCT GCAAGTGGAT GGGGATCTGC AACTTCAATC 601 AATCAACTTC ATCGGAGGCC AGCCCCTCAC CTTCAACCCG CCTGTGCCAT ATTTCGGGAG 661 GCTGCAAGGA GGGCTCACAG CTCGAAGAAC CATCATCATC AAGGGCTATG TGCCTCCCAC 721 AGGCAAGAGC TTTGCTATCA ACTTCAAGGT GGGCTCCTCA GGGGACATAG CTCTGCACAT 781 TAATCNNCGC ATGGGCAACG GTACCGTGGT CCGGANNAGC CTTCTGAATG GCTCGNGGGG 841 NNNCGANGAN AAGAAGANCA CCCACAACCC ATTTGGTCCC GGANAGNNCT TTGATCTGNN 901 NNTNCGCTGT GGCTNNNNNC GCTTCANGGN TTANGCCNNN NNNNAGCACC NCTTTGANTT 961 TGCCCATCNN NNNNNGGCNN TNNNNANGGN NNNNNNNTTN NNAANTNCNN GGNNNNNNNN 1021 NN //
Primer: pET-check-R1 (Pr0192) Region: T7 ter, insert 3' Sequence file: RDB19438_B1Kqb.seq
>D08453A5_B1Kq_2_pET-check-R1_H01_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN AACGNAGNCN GGCACCGTGN ATGAAATCTA 61 ACAATGCGCT CATCGTCATC CTCGGCACCG TCACCCTGGA TGCTGTAGGC ATAGGCTTGG 121 TTATGCCGGT ACTGCCGGGC CTCTTGCGGG ATATCCGGAT ATAGTTCCTC CTTTCAGCAA 181 AAAACCCCTC AAGACCCGTT TAGAGGCCCC AAGGGGTTAT GCTAGTTATT GCTCAGCGGT 241 GGCAGCAGCC AACTCAGCTT CCTTTCGGGC TTTGTTAGCA GCCGGATCTT TAGATTTGGA 301 CATAGGACAA GGTGACATCA CCCTGGATTT CCAATGTGTC CACCCTCTGG AAGGCCGAGA 361 GGCGATGGGC AAAGTCAAAG AGGTGCTGGC CATTGGCGTA AACCTTGAAG CGATCCAAGC 421 CACAGCGAAT GGACAGATCA AAGAACTGTC CGGGACCAAA TGGGTTGTGG GTGATCTTCT 481 TCTCCTCGGA TCCCCACGAG CCATTCAGAA GGCTGTTCCG GACCACGGTA CCGTTGCCCA 541 TGCGGGGATT AATGTGCAGA GCTATGTCCC CTGAGGAGCC CACCTTGAAG TTGATAGCAA 601 AGCTCTTGCC TGTGGGAGGC ACATAGCCCT TGATGATGAT GGTTCTTCGA GCTGTGAGCC 661 CTCCTTGCAG CCTCCCGAAA TATGGCACAG GCGGGTTGAA GGTGAGGGGC TGGCCTCCGA 721 TGAAGTTGAN TGATTGAAGT TGCAGATCCC CATCCACTTG CAGGTGGGGT GACCATCTGT 781 AGGGGAAGCC GGTGCCCGTA CTCATAGAAN GGATTTCCNN TTNNCACCNC CTTGTANTGC 841 TCANCCNGGA CTATGANNAC NAGCTCAAAN NNGGCACCCT TTTTNAANNG CATGCTCCNN 901 TTCNTNTNCT CGCTGNCCCC ACTTCCCNNC CTGCNNCGNG NTTGAANNNN NNNNTTGNCC 961 CNNCCNNNNN AANNCGCGGN NTGAAGTGGN ANNGNNANGT NNNNANCCNN GGNNCCNNNC 1021 NNNNNNNNNA ANNNTCNNNN NANNANCNNN NNNNNNN //
Primer: lacIpro_F (Pr0409) Region: lacI pro, lacI Sequence file: RDB19438_B1Kqc.seq
>D08453A5_B1Kq_2_lacIpro_F_C03_09_ABI24.ab1 1 NNNNNNNNNN NNNNNCGNCN NNNNANANTC NATTCNGGGT GGTGAATGTG AAACCAGTAA 61 CGTTATACGA TGTCGCAGAG TATGCCGGTG TCTCTTATCA GACCGTTTCC CGCGTGGTGA 121 ACCAGGCCAG CCACGTTTCT GCGAAAACGC GGGAAAAAGT GGAAGCGGCG ATGGCGGAGC 181 TGAATTACAT TCCCAACCGC GTGGCACAAC AACTGGCGGG CAAACAGTCG TTGCTGATTG 241 GCGTTGCCAC CTCCAGTCTG GCCCTGCACG CGCCGTCGCA AATTGTCGCG GCGATTAAAT 301 CTCGCGCCGA TCAACTGGGT GCCAGCGTGG TGGTGTCGAT GGTAGAACGA AGCGGCGTCG 361 AAGCCTGTAA AGCGGCGGTG CACAATCTTC TCGCGCAACG CGTCAGTGGG CTGATCATTA 421 ACTATCCGCT GGATGACCAG GATGCCATTG CTGTGGAAGC TGCCTGCACT AATGTTCCGG 481 CGTTATTTCT TGATGTCTCT GACCAGACAC CCATCAACAG TATTATTTTC TCCCATGAAG 541 ACGGTACGCG ACTGGGCGTG GAGCATCTGG TCGCATTGGG TCACCAGCAA ATCGCGCTGT 601 TAGCGGGCCC ATTAAGTTCT GTCTCGGCGC GTCTGCGTCT GGCTGGCTGG NATAAATATC 661 TCNCTCGCAA TCNAATTCNN NNGANNNCNN NANGGGANNN NNANNNNNNN NNN //