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pUCtk2x2 EEF1-TEZ (#RDB19571)

Donor vector for the tdTomato reporter knock-in at the ORF end of EEF1A1.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Nakade, K., Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name pUCtk2x2 EEF1-TEBl
Clone info. Donor vector for the tdTomato reporter knock-in at the ORF end of EEF1A1. Selection of knocked-in clone using the double-tk system is available. Left side HSV TK contains c.203T>C (Leu>Pro), tdTomato contains c.467G>A (Gly>Asp).
Vector backbone pUCtk2x2 (RDB19569) (plasmid)
Gene/insert name Herpes simplex virus (HSV) thymidin kinase (TK) gene; human EEF1A1 genomic DNA; Dscosoma sp. red fluorescent protein (tdTomato); human EEF1A1 promoter; Streptoalloteichus hindustanus bleomycin resistant gene.
Depositor|Developer DNA Bank, | Nakade, Koji |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested.　(Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Nakade, K. et al., Cell Rep. Methods 3 (12): 100662 (2023))。

Catalog # Resource name Shipping form Fee
RDB19571 pUCtk2x2 EEF1-TEZ DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The pUCtk2x2 EEF1-TEZ was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19571).

Reference section:

Nakade, K., Tsukamoto, S., Nakashima, K., An, Y., Sato, I., Li, J., Shimoda, Y., Hemmi, Y., Miwa, Y., Hayashi, Y., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Nakade, K., Efficient selection of knocked-in pluripotent stem cells using a dual cassette cellular elimination system. Cell Rep. Methods 3 (12): 100662 (2023). PMID 38086384. [PubMed] [Article] [RRC of NBRP]