Please review the QC test results indicated by icon below as well as clone information before placing your order.

pEGFP-C1-hTBC1D18-R584K 

Retroviral expression vector of human TBC1D18 GAP activity-deficient mutant tagged with EGFP at N-terminus.

Sequence (full)	RDB19705zzt01.seq compiled by us

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Itoh, T. et al. Genes Cells. 11, 1023-1037, 2006)

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Please review the QC test results indicated by icon below as well as clone information before placing your order.

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB19705_B2Jjp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EGFP-C2 (Pr0402) Region: EGFP 3',insert 5' Sequence file: RDB19705_B2Jja.seq
>D09032A2_B2Jj_1_EGFP-C2_D01_10_ABI24.ab1     1 NNNNNNNNNN NNNNCNNGNN CNCNTGGTCC TGCTGGAGTT CGTGACCGCC GCCGGGNTCA    61 CTCTCGGCAT GGANGAGCTG TACAAGTCCG GACTCAGATC CATGGAGGTC AGAGCTTCAT   121 TACAGAAGGT TAGTGGATCA TCTGATTCTG TGGCTACAAT GAACAGTGAA GAATTTGTTT   181 TGGTTCCTCA GTATGCAGAT GATAATTCTA CAAAACATGA AGAAAAACCT CAACTGAAGA   241 TAGTTTCTAA TGGTGATGAA CAATTGGAAA AAGCCATGGA AGAGATTTTG AGAGATTCCG   301 AGAAAAGGCC AAGCAGTCTT CTTGTTGATT GTCAAAGTTC CAGTGAGATT TCAGACCATT   361 CGTTTGGAGA TATTCCAGCC AGCCAAACAA ATAAGCCATC TCTTCAGTTA ATTTTGGATC   421 CGTCTAACAC AGAAATTTCT ACACCCAGAC CATCTTCTCC AGGTGGACTA CCTGAAGAAG   481 ATAGTGTTTT ATTTAATAAA CTGACCTACT TAGGATGTAT GAAGGTTTCT TCCCCACGTA   541 ATGAAGTAGA GGCTTTACGG GCAATGGCAA CCATGAAATC TTCCAGTCAA TACCCCTTTC   601 CTGTTACCCT GTATGTACCA AATGTTCCAG AAGGTTCTGT GAGAATTATA GACCAATCCA   661 GCAATGTGGA GATAGCATCT TTTCCAATCT ATAAGGTGTT ATTCTGTGCA CGTGGACATG   721 ACGGAACAAC AGAGAGCAAT TGCTTTGCAT TTACAGAGAG TTCCCATGGT TCGGAAGAAT   781 TTCANATACA TGTTTTCTCC TGTGAAATTA AAGANGCAGT AAGCAGAATT TTGTACAGTT   841 TCTGTACAGC ATTCAAACGT TCTTCCAGAC AANNGTCTGA NGTTAAAGAN TCAGTTATTC   901 CTACCCCCGA CAGTGATGTG TTTACCTTCA GTGTCTCCTT GGANGTAAAA GAANANNANN 961 GNAAANGAAA CTTTANCCNT GNNNNNNNNN N //
Primer: SV40polyA_R2 (Pr0623) Region: insert 3' Sequence file: RDB19705_B2Jjb.seq
>D09032A2_B2Jj_1_SV40polyA_R2_E01_13_ABI24.ab1     1 NNNNNNNNNN NNNCNGNANN ANNNNNNTAA CNACNACNAT TGCATTCATT TTATGTTTCN    61 GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA AATGTGGTAT   121 GGCTGATTAT GATCAGTTAT CTAGATCCGG TGGATCCCGG GCCCGCGGTA CCGTCGACCT   181 GCAGGCGGCC GCGAATTCAC TAGTGATTAC AGCTGGAGTT ACCTACAAAT ATACAAACTT   241 GTATCTATCC ATTGGGTCTT CCTGTTGCAG CTGACTCTCT CGCATTGTCT GATATTCTTT   301 CTCATATTTC TTCAGCTTCT TGGTTGGTAC TTTAATATTG CAAGCCTGCT CCATCAGTCT   361 TCTTGCATTT TCCTCTGCCC TGTATCTCTT TGGAAGCTGA ACTCTAAAGA ACTTTAAAGC   421 ACCTTCAAAA TCAGCCTGCA GAAGGTCTTC CTTTGAGGTC TTTAGGAGAG CCAAAGCTAC   481 ATGAAAGATT ATGTTCAAAC CCTCACAAAG CAGTAAGTCA ATGATGTGGA ACACCATGCA   541 GAGTGGGAAC TTGGCAGTAA AAAGAGTGAG AAACCACTGG GATGCATACA TATGAGCTTC   601 CAGGTTCAGA TCAGAAAAAT GGCTATGCAG GTCCGGTAGC TGTTCCTGCA TTAGTCTCTC   661 CAACTGGTAG AATTTGCAAT GAAGATCTTC GAAGTTGTTT CTGTAGAGGT CTCTCAAACC   721 ATAGTCGTAC ATGATTTTCA CCAAAACACA GAATGCTTGT TCCTCTGGCA TATGCAGCAG   781 TAATACAGCA GCAAGAAAAG ANTGCCCTTG ACAGTACCCA ATGTCTTCAT CATACACAGA   841 GTANGCCTTG CAGATCTTAT AGAGCGATTC TTGACCATCT CCTCCAGTAT CTTTAAAGTA   901 ATCNTGTGCG GGAAATGTCT TATGAATATC TCGAGTAANN NCACTCTCCT NGGCTGANTC   961 CTTTGTGANN AGAATTCNGN ATCTATCCNG CANTGCCNNN NNGNCATNGN CANNCNGNCN 1021 NNTAACTGNN NNANNNNNNN CCNNNNNNGN NTNNNNNGNN NNNNNNNN //
Primer: hTBC1D18_mid_R (Pr1294) Region: insert mid Sequence file: RDB19705_B2Jjc.seq
>D09032A2_B2Jj_1_hTBC1D18_mid_R_A03_03_ABI24.ab1     1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNG AANGCTTGTT CCTCTGGCAT ATGCAGCAGT    61 AATACAGCAG CAAGAAAAGA CTGCCCTTGA CAGTACCCAA TGTCTTCATC ATACACAGAG   121 TAGGCCTTGC AGATCTTATA GAGCGATTCT TGACCATCTC CTCCAGTATC TTTAAAGTAA   181 TCATGTGCGG GAAATGTCTT ATGAATATCT CGAGTAATAA CACTCTCCTG GGCTGAGTCC   241 TTTGTGATAA GAATTCGGTA TCTATCCAGC ATTGCCTGGT TGTCATGGCA GCCTGCCAAT   301 AACTGCCATA CCTCTGCCCT CAATGCTTCA GGGACACCAC TCTTCACCAG AGTAGACAGC   361 CCTTTCGGTC GTGCACCAAG GTTACTGTGC CATTTTCCTA GCAACTCTCC CCAAGAATAC   421 AGGATCTTCT CAGGACAATC CTTAGACACA TCACCTGTTC CACTTGAGAG TTCATTATCA   481 CTCTCCTCTT CTGCTTCATC ATCCTGGGGT GACATTGGAN CCCCTCCACT AGTANGAGTG   541 ACTGGTTCCT CCTTGTCAGA NTCTCGCTGT AGACTCACCA CCTCATATAT TGCATCTCCA   601 GCNNTGNTAT GGNCTTTTCC NTCANANNGN TTCNATCTCN TGAAGAAAGT CTCTGTGAAA   661 NTCNTTCTGC TGAAATANCA AANTCGCTCA TTTGNNNGGN ANNNACNGNN TANTGNCTNC 721 NNNNNNAAGC NANNNGNNTN CNNNNNNTCN GNGANNNNNN TATCCNNNNN NNNNN //
Primer: CMV_Forward (Pr0016) Region: CMV pro,EGFP 5' Sequence file: RDB19705_B2Jjd.seq
>D09032A2_B2Jj_1CMV_Forward_H01_08_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN NTTGNNNCNA ATGGGCGGNA GGCGTGTACG GTGGGAGGTC    61 TATATAAGCA GAGCTGGTTT AGTGAACCGT CAGATCCGCT AGCGCTACCG GTCGCCACCA   121 TGGTGAGCAA GGGCGAGGAG CTGTTCACCG GGGTGGTGCC CATCCTGGTC GAGCTGGACG   181 GCGACGTAAA CGGCCACAAG TTCAGCGTGT CCGGCGAGGG CGAGGGCGAT GCCACCTACG   241 GCAAGCTGAC CCTGAAGTTC ATCTGCACCA CCGGCAAGCT GCCCGTGCCC TGGCCCACCC   301 TCGTGACCAC CCTGACCTAC GGCGTGCAGT GCTTCAGCCG CTACCCCGAC CACATGAAGC   361 AGCACGACTT CTTCAAGTCC GCCATGCCCG AAGGCTACGT CCAGGAGCGC ACCATCTTCT   421 TCAAGGACGA CGGCAACTAC AAGACCCGCG CCGAGGTGAA GTTCGAGGGC GACACCCTGG   481 TGAACCGCAT CGAGCTGAAG GGCATCGACT TCAAGGAGGA CGGCAACATC CTGGGGCACA   541 AGCTGGAGTA CAACTACAAC AGCCACAACG TCTATATCAT GGCCGACAAG CAGAAGAACG   601 GCATCAAGGT GAACTTCAAG ATCCGCCACA ACATCGAGGA CGGCAGCGTG CAGCTCGCCG   661 ACCACTACCA GCAGAACACC CCCATCGGCG ACGGCCCCGT GCTGCTGCCC GACAACCACT   721 ACCTGAGCAC CCAGTCCGCC CTGAGCAAAG ACCCCAACGA GAAGCGCGAT CACATGGTCC   781 TGCTGGAGTT CGTGACCGCC NNNNNNATCA CTCTCGGCAT GGACGAGCTG TACNANGTCC   841 GGACTCNGAT NCATGGAGGT CANANCTTCN TTACAGAAGG NTAGTGGATC ATCTGATTCT   901 GTGGCTACNN NGAANAGNGA ANANNTTGNT TTGGNNCCTC AGTATGCAGA TGANAATTCT   961 ACAAAANNTG ANNNAAANNN TCNNCTGANN NNANNTTCNA ATNGNGNNGN ACNNTTGGAA 1021 AAANNNANNG NNNNANNNTT TGANNNNNNN NNN //
Primer: VN_F1 (Pr0992) Region: EGFP 3',insert 5' Sequence file: RDB19705_B2Jje.seq
>D09032A2_B2Jj_1_VN_F1_F02_06_ABI08.ab1     1 NNNNNNNNNN NNNNNNNNNN GCNNNNNNNT CTATATNNTG GCCGANAAGC AGAAGAACGG    61 CATCAAGGTG AACTTCAAGA TCCGCCACAA CATCGAGGAC GGCAGCGTGC AGCTCGCCGA   121 CCACTACCAG CAGAACACCC CCATCGGCGA CGGCCCCGTG CTGCTGCCCG ACAACCACTA   181 CCTGAGCACC CAGTCCGCCC TGAGCAAAGA CCCCAACGAG AAGCGCGATC ACATGGTCCT   241 GCTGGAGTTC GTGACCGCCG CCGGGATCAC TCTCGGCATG GACGAGCTGT ACAAGTCCGG   301 ACTCAGATCC ATGGAGGTCA GAGCTTCATT ACAGAAGGTT AGTGGATCAT CTGATTCTGT   361 GGCTACAATG AACAGTGAAG AATTTGTTTT GGTTCCTCAG TATGCAGATG ATAATTCTAC   421 AAAACATGAA GAAAAACCTC AACTGAAGAT AGTTTCTAAT GGTGATGAAC AATTGGAAAA   481 AGCCATGGAA GAGATTTTGA GAGATTCCGA GAAAAGGCCA AGCAGTCTTC TTGTTGATTG   541 TCAAAGTTCC AGTGAGATTT CAGACCATTC GTTTGGAGAT ATTCCAGCCA GCCAAACAAA   601 TAAGCCATCT CTTCAGTTAA TTTTGGATCC GTCTAACACA GAAATTTCTA CACCCAGACC   661 ATCTTCTCCA GGTGGACTAC CTGAAGAAGA TAGTGTTTTA TTTAATAAAC TGACCTACTT   721 AGGATGTATG AAGGTTTCTT CCCCACGTAA TGAAGTAGAG GCTTTACGGG CAATGGCAAC   781 CATGAAATCT TCCAGTCNAT ACCCCTTTCC TGTTACCCTG TATGTACCAA ATGTTCCAGA   841 ANGTTCTGTG AGANNTATAG ACNNNTCNAG CAATGTGGAN ATAGCATCTT TTCNNATCTA   901 TAANGNGTTA TTCTGTGCAC GTGGNNNNTG NCGGAACNAC NNAGAGCNAT TGCTTTGCNT   961 TTACAGANAG TTNCNANGGN TNCGNAANAA TTTNNNANAC NTGNTTTCNN NNNNGAAATT 1021 NAANNNNNNN NNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

2023.05.22

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