Resource data sheet
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Kohinoor 2.0/pcDNA3 (#RDB19862)

Vector of Kohinoor 2.0, a photoswitchable fluorescent protein, for mammalian expression.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format
SnapGene file
Publication Wazawa, T., Microscopy (Oxf). 70 (4): 340-352 (2021). PMID 33481018. [link to RRC of NBRP]
Test sheet Data Sheet 
 
Clone info. Vector of Kohinoor 2.0, a photoswitchable fluorescent protein, for mammalian expression.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Growth conditions LB+Amp, 37oC
Gene/insert name Echinophyllia sp. SC22 GFP (Kohinoor 2.0).
Depositor|Developer Nagai, Takeharu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wazawa, T. et al., Microscopy, 70 (4): 340-352, 2021) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wazawa, T. et al., Microscopy, 70 (4): 340-352, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。


Catalog # Resource name Shipping form Fee
RDB19862 Kohinoor 2.0/pcDNA3 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The Kohinoor 2.0/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19862).

Reference section:

Wazawa, T., Noma, R., Uto, S., Sugiura, K., Washio, T., Nagai, T., A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging. Microscopy (Oxf). 70 (4): 340-352 (2021). PMID 33481018. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Wazawa, T., A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging. Microscopy (Oxf). 70 (4): 340-352 (2021). PMID 33481018. [link to RRC of NBRP]