Resource data sheet
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Kohinoor 2.0/pcDNA3

Vector of Kohinoor 2.0, a photoswitchable fluorescent protein, for mammalian expression.

Catalog number RDB19862
Resource name Kohinoor 2.0/pcDNA3
Clone info. Vector of Kohinoor 2.0, a photoswitchable fluorescent protein, for mammalian expression.
Vector backbone pcDNA3 (plasmid)
Size of vector backbone 5.4 kb
Selectable markers Ampicillin (E. coli), Neomycin (mammalian cell)
Gene/insert name Echinophyllia sp. SC22 GFP (Kohinoor 2.0).
Depositor|Developer Nagai, Takeharu |
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External Database
Echinophyllia sp. SC22 GFP (Kohinoor 2.0)

            Reference sequence
              
             
            Sequence (full) RDB19862hts01.seqcheck assembled from experimentally sequenced data
            Test sheet RDB19862_B3Acp1-1.pdf 

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Wazawa, T. et al., Microscopy, 70 (4): 340-352, 2021) The RECIPIENT which wants to use the BIOLOGICAL RESOURCE for the purpose other than education or not-for-profit research is requested to enter into a Material Transfer Agreement with Osaka University.
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            MTA, for use for not-for-profit academic purpose [Word]
            MTA, for use for for-profit purpose [Word]
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Wazawa, T. et al., Microscopy, 70 (4): 340-352, 2021)。利用者が非営利目的の教育・研究以外の目的に用いる場合は、大阪大学と別途MTAを締結すること。

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            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB19862 Kohinoor 2.0/pcDNA3 DNA solution


            How to cite this biological resource

            Materials & Methods section:

            The Kohinoor 2.0/pcDNA3 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB19862).

            Reference section:

            Wazawa, T., Noma, R., Uto, S., Sugiura, K., Washio, T., Nagai, T., A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging. Microscopy (Oxf). 70 (4): 340-352 (2021). PMID 33481018. [link to RRC of NBRP]

            Further references such as user reports and related articles (go to bottom)


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            References

            Original, user report and related articles

            original Wazawa, T., A photoswitchable fluorescent protein for hours-time-lapse and sub-second-resolved super-resolution imaging. Microscopy (Oxf). 70 (4): 340-352 (2021). PMID 33481018. [link to RRC of NBRP]

            2024.04.11

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