Resource data sheet
DNA Bank Top

pBS Coupler4/td8ox2StayGold (c4) (#RDB20226)

Plasmid vector of td8ox2StayGold(c4) for fusion to the N-terminus of a target molecule (X) through a Coupler linker: (GGGGS)x3.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Ando, R., Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. For fusion of td8ox2StayGold(c4) to the N-terminus of a target molecule (X) through a Coupler linker: (GGGGS)x3. td8ox2StayGold is an intramolecular dimeric unit that performs as a monomer, and is composed of n1, oxStayGold(Y169F), PT, a 21-residue linker, n1, oxStayGold(Y169F), and c4. n1 is the appendage that facilitates fusions to the N-terminus of oxStayGold(Y169F). PT and c4 are the appendages that facilitate fusions to the C-terminus of oxStayGold(Y169F). td8ox2StayGold(c4) encoded by this plasmid DNA is followed by a Coupler linker: (GGGGS)×3. See, Supp. Fig.14 of Ando, R. et al. Nat. Methods, 2023. https://doi.org/10.1038/s41592-023-02085-6 for more information.
Vector backbone pBS Coulper4 (RDB15129) (plasmid)
Growth conditions LB+Amp, 37oC
Gene/insert name Cytaeis uchidae GFP (StayGold) cDNA.
Depositor|Developer Miyawaki, Atsushi | Ando, Ryoko |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Ando, R. et al., Nat. Methods 21 (4): 648-656 (2024).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 1. If the BIOLOGICAL RESOURCE is to be used by a for-profit organization, the RECIPIENT must agree with RIKEN Innovation Co., Ltd. on the terms and conditions of its use. For inquiries, please contact:
license-contact@innovation-riken.jp
2. The RECIPIENT’s Scientist agrees not to transfer the BIOLOGICAL RESOURCE and its derivatives to any third party including any resource center without the prior written consent of DEPOSITOR.
3. When publishing the research results obtained using the BIOLOGICAL RESOURCE, a citation to the literature specified by the DEPOSITOR or an acknowledgement to the DEPOSITOR is requested.
Ando, R. et al., Nat. Methods 21 (4): 648-656 (2024).

Catalog # Resource name Shipping form Fee
RDB20226 pBS Coupler4/td8ox2StayGold (c4) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pBS Coupler4/td8ox2StayGold (c4) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20226).

Reference section:

Ando, R., Shimozono, S., Ago, H., Takagi, M., Sugiyama, M., Kurokawa, H., Hirano, M., Niino, Y., Ueno, G., Ishidate, F., Fujiwara, T., Okada, Y., Yamamoto, M., Miyawaki, A., StayGold variants for molecular fusion and membrane-targeting applications. Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Ando, R., StayGold variants for molecular fusion and membrane-targeting applications. Nat. Methods 21 (4): 648-656 (2024). PMID 38036853. [PubMed] [Article] [RRC of NBRP]