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pRS424-GPDp BiPN- PssA codon.opt-HDEL. (#RDB20397)

Expression vector of BiPN- PssA-HDEL with the GPD promoter and CYC1 terminator. Multi copy. TRP1 marker.

Clone info. Expression vector of BiPN- PssA-HDEL with the GPD promoter and CYC1 terminator. Multi copy. TRP1 marker.
Comment Expression was confirmed by the depositor with western blotting.
Vector backbone pRS424 (plasmid)
Growth conditions LB+Amp, 37oC
Gene/insert name Saccharomyces cerevisiae KAR2; Escherichia coli pssA.
Depositor|Developer Tamura, Yasushi |
 
Sequence (full) RDB20397hts01.seq checkAssembled from experimentally sequenced data.
Publication Shiino, H., FEBS J. 288 (10): 3285-3299 (2021). [link to RRC of NBRP]
Test sheet RDB20397C0p1-1.pdf 
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Shiino et al., FEBS J. 288(10), 3285-3299, 2020) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the Yamagata University.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Shiino et al., FEBS J. 288(10), 3285-3299, 2020)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、山形大学の許可を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20397 pRS424-GPDp BiPN- PssA codon.opt-HDEL. DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pRS424-GPDp BiPN- PssA codon.opt-HDEL. was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20397).

Reference section:

Shiino, H., Furuta, S., Kojima, R., Kimura, K., Endo, T., Tamura, Y., Phosphatidylserine flux into mitochondria unveiled by organelle-targeted Escherichia coli phosphatidylserine synthase PssA. FEBS J. 288 (10): 3285-3299 (2021). PMID 33283454. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Shiino, H., Phosphatidylserine flux into mitochondria unveiled by organelle-targeted Escherichia coli phosphatidylserine synthase PssA. FEBS J. 288 (10): 3285-3299 (2021). PMID 33283454. [link to RRC of NBRP]