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pET-hMetAP1D(44–335) (#RDB20441)

E. coli expression vector of human N-terminal truncated MAP12 (44–335).

Clone info. E. coli expression vector of human N-terminal truncated MAP12 (44–335). The resulting recombinant hMetAP1D protein is tagged with the N-terminal His6-tag.
Vector backbone pET-28b(+) (plasmid)
Selectable markers Kanamycin
Growth conditions LB+Kanamycin, 37℃
Gene/insert name human METAP1D cDNA.
Depositor|Developer Kim, Hyoun Sook |
 
Sequence (full) RDB20441hts01.seq checkAssembled from experimentally sequenced data.
Publication Lee, Y., Sci. Rep. 13 (1): 22326 (2023). [link to RRC of NBRP]
Test sheet RDB20441C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(Lee, Y. et al., Sci. Rep. 13 (1): 22326, 2023) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the National Cancer Center Korea.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Lee, Y. et al., Sci. Rep. 13 (1): 22326, 2023)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、National Cancer Center Koreaの許可を得ること。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB20441 pET-hMetAP1D(44–335) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pET-hMetAP1D(44–335) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20441).

Reference section:

Lee, Y., Kim, H., Lee, E., Hahn, H., Heo, Y., Jang, D.M., Kwak, K., Kim, H.J., Kim, H.S., Structural insights into N-terminal methionine cleavage by the human mitochondrial methionine aminopeptidase, MetAP1D. Sci. Rep. 13 (1): 22326 (2023). PMID 38102161. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Lee, Y., Structural insights into N-terminal methionine cleavage by the human mitochondrial methionine aminopeptidase, MetAP1D. Sci. Rep. 13 (1): 22326 (2023). PMID 38102161. [link to RRC of NBRP]