RDB20462 - pCRtk

pCRtk_SOX10-TEZ (#RDB20462)

Donner vector to tag T2A-tdTomato at human SOX10, C-terminaly.

Drawn by SnapGene® software

Sequence information (Assembled from experimentally sequenced data)	GenBank Flat File Format open
	SnapGene file download
Publication	Unpublished bioresource
Test sheet	Data Sheet open

Clone info.	Genome editing tool. Donner vector to tag T2A-tdTomato at human SOX10, C-terminaly. Selection with Zeo and GCV (caution: tdTomato point mutation including)
Vector backbone	pCR-BluntII-TOPO (plasmid)
Growth conditions	LB + 25 µg/mL Zeocin 37dC
Gene/insert name	human SOX10 genomic DNA; T2A; tdTomato; bGH poly(A); CMV promoter; human EF-1α promoter; Streptoalloteichus hindustanus zeocin resistance gene; CMV EI promoter HSV thymidine kinase cDNA, poly(A).
Depositor\|Developer	DNA Bank, \| Nakashima, Kenichi \|

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Catalog #	Resource name	Shipping form	Fee
RDB20462	pCRtk_SOX10-TEZ	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein