RDB20663 - Lenti-pCSII-CMV-PPAP2.2-IRES-puro(Amp)

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Lenti-pCSII-CMV-PPAP2.2-IRES-puro(Amp) (#RDB20663)

Lentivirus vector plasmid. To visualize half of the PPAP2 moieties, the mCherry fluorescent proteins were fused to iSH2-SspB (PPAP2.2).

Clone info.	Lentivirus vector plasmid. To visualize half of the PPAP2 moieties, the mCherry fluorescent proteins were fused to iSH2-SspB (PPAP2.2). See Fig.1B of Ueda, Y. et. al., Cell Chem. Biol., 29 (11) :1576-1587, 2022 (DOI: 10.1016/j.chembiol.2022.10.002).
Vector backbone	CSII-CMV-MCS derivative (plasmid)
Size of vector backbone	BamHI/XhoI
Growth conditions	LB+Amp, 37oC
Gene/insert name	Bos taurus PIK3R1 (inter-SH2 domain); Haemophilus influenzae stringent starvation protein B (SspB); Discosoma sp. RFP (mCherry); porcine teschovirus self-cleavage sequence (P2A); Thosea asigna self-cleavage sequence (T2A); Avena sativa phototropin-1 (LOV2 domain); E. coli SsrA; human KRAS (CAAX motif).
Depositor\|Developer	Ueda, Yoshibumi \|

Sequence (full)	RDB20663hts01.seq Assembled from experimentally sequenced data.
Publication	Ueda, Y., Cell Chem. Biol. 29 (11): 1576-1587.e5 (2022). [link to RRC of NBRP]
Test sheet	RDB20663C0p1-1.pdf

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Ueda, Y et al, Cell Chem. Biol. 29(11):1576-1587.e5. 2022)
Additional terms and conditions:
Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] In order to receive this biological resource, please request the Exclusive MTA to us. Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Ueda, Y et al, Cell Chem. Biol. 29(11):1576-1587.e5. 2022) Additional terms and conditions: Regarding lentivirus vector: The RECIPIENT agrees to expressly describe Dr. Hiroyuki Miyoshi as the Developer of the lentivirus vector. The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit academic purpose.
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 本件遺伝子材料の依頼に際し、専用MTA(英語版のみ)の提出をお願いします。当室にご請求ください。遺伝子組換え生物の受入れ確認書が必要です。当室にご請求ください。手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Ueda, Y et al, Cell Chem. Biol. 29(11):1576-1587.e5. 2022) 。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、国立感染症研究所の許可を得ること。付加的使用条件：レンチウイルスベクターについて：本件研究材料を利用した研究結果等を発表する際は、レンチウイルスベクターが三好浩之博士により開発されたことを明示する。本件リソースの使用は学術機関での学術研究に限る。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB20663	Lenti-pCSII-CMV-PPAP2.2-IRES-puro(Amp)	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

How to cite this biological resource

Materials & Methods section:

The Lenti-pCSII-CMV-PPAP2.2-IRES-puro(Amp) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20663).

Reference section:

Ueda, Y., Miura, Y., Tomishige, N., Sugimoto, N., Murase, M., Kawamura, G., Sasaki, N., Ishiwata, T., Ozawa, T., Mechanistic insights into cancer drug resistance through optogenetic PI3K signaling hyperactivation. Cell Chem. Biol. 29 (11): 1576-1587.e5 (2022). PMID 36288730. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original	Ueda, Y., Mechanistic insights into cancer drug resistance through optogenetic PI3K signaling hyperactivation. Cell Chem. Biol. 29 (11): 1576-1587.e5 (2022). PMID 36288730. [link to RRC of NBRP]