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DEST40/PAmCherry-GRP1-PH (#RDB20667)

Expression vector of the human GRP1 PH domain, which interacts with PI(3,4,5)P3 tagged with PAmCherry at the N terminus.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Abe, M., Elife 13: e101652 (2024). PMID 39513999. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Clone info. Expression vector of the human GRP1 PH domain, which interacts with PI(3,4,5)P3. A photoactivatable red fluorescent protein, PAmCherry, was fused to the N terminus.
Vector backbone pcDNA-DEST40 (plasmid)
Selectable markers Ampicillin
Gene/insert name human CYTH3 cDNA; Discosoma sp. RFP (PAmCherry1).
Depositor|Developer Abe, Mitsuhiro |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Abe, M. et al., eLife, Nov 8, 2024 https://doi.org/10.7554/eLife.101652) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the RIKEN CPR.
Additional terms and conditions:
Regarding PAmCherry: This plasmid will only be provided for academic research at not-for-profit institution. When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Vladislav V. Verkhusha is requested.
Subach, F.V. et al., Nat. Methods. 6 (4): 311 (2009).
Remarks
Gateway® Expression clone. Please refer Life Technologies Corporation signs license agreement with RIKEN BRC.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abe, M. et al., eLife, Nov 8, 2024 https://doi.org/10.7554/eLife.101652)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、理研CPRの許可を得ること。
付加的使用条件：
Regarding PAmCherry: This plasmid will only be provided for academic research at not-for-profit institution. When publishing the results obtained using the BIOLOGICAL RESOURCE, a citation of literature specified by Dr. Vladislav V. Verkhusha is requested.
Subach, F.V. et al., Nat. Methods. 6 (4): 311 (2009).
備考
Gateway®テクノロジーを用いた発現クローンです。「Gateway®テクノロジーを用いたクローンの提供と寄託のご案内」をご覧ください。

Catalog # Resource name Shipping form Fee
RDB20667 DEST40/PAmCherry-GRP1-PH DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for Gateway® expression clone

How to cite this biological resource

Materials & Methods section:

The DEST40/PAmCherry-GRP1-PH was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20667).

Reference section:

Abe, M., Yanagawa, M., Hiroshima, M., Kobayashi, T., Sako, Y., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Abe, M., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. [PubMed] [Article] [RRC of NBRP]