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pCMV-EGFR(WT)-Halo (#RDB20672)

Expression vector of human wild-type EGFR. HaloTag(R) 7 was fused to the C terminus.

Alternative name EGFR(WT)–Halo
Clone info. Expression vector of human wild-type EGFR. HaloTag(R) 7 was fused to the C terminus.
Vector backbone pEGFP-N1 (plasmid)
Selectable markers Kanamycin (E. coli), Neomycin (mammalian cell)
Gene/insert name human EGFR cDNA; Rhodococcus rhodochrous dehalogenase (HaloTag7)
Depositor|Developer Abe, Mitsuhiro |
 
Sequence (full) RDB20672hts01.seq checkAssembled from experimentally sequenced data.
Publication Abe, M., Elife 13: e101652 (2024). PMID 39513999. [link to Journal]
Test sheet RDB20672C0p1-1.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
MTA, for use for for-profit purpose [Word]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. (Abe, M. et al., eLife, Nov 8, 2024 https://doi.org/10.7554/eLife.101652) For use of the BIOLOGICAL RESOURCE by a for-profit organization or for use of the BIOLOGICAL RESOURCE for for-profit research by not-for-profit organization, the RECIPIENT must obtain a prior written consent from the RIKEN CPR.
Additional terms and conditions:
The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees tobe bound by the conditions of the limited use statement of the Promega Corporation.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(Abe, M. et al., eLife, Nov 8, 2024 https://doi.org/10.7554/eLife.101652)。営利機関の利用もしくは非営利学術機関による営利目的研究利用の場合は、理研CPRの許可を得ること。
付加的使用条件：
本件リソースの使用は学術機関での学術研究に限る。本件リソースの使用にあたって、利用者はプロメガ社の限定使用条件に従う必要がある。

Catalog # Resource name Shipping form Fee
RDB20672 pCMV-EGFR(WT)-Halo DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pCMV-EGFR(WT)-Halo was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB20672).

Reference section:

Abe, M., Yanagawa, M., Hiroshima, M., Kobayashi, T., Sako, Y., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. [link to Journal]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Abe, M., Bilateral regulation of EGFR activity and local PI(4,5)P2 dynamics in mammalian cells observed with superresolution microscopy. Elife 13: e101652 (2024). PMID 39513999. [link to Journal]