RDB01675 - pxCANCre

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

pxCANCre (#RDB01675)

A plasmid expressing the recombinase Cre tagged with a nls

Clone info.	A plasmid expressing the recombinase Cre tagged with a nuclear localization signal under the control of CAG promoter
Vector backbone	pxCAwt (deleted from pAxCAwt with Sal I) (Plasmid)
Size of vector backbone	6,565 bp
Selectable markers	Amp^r
Gene/insert name	P1 phage Cre recombinase cDNA
Depositor\|Developer	Saito, Izumu \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.

Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)).
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995)). Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

必要書類

提供依頼書

[依頼書の記入例

]
提供同意書 (MTA, 非営利学術目的用)[Word

] [MTAの記入例

]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。

MTAに書く使用条件

利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Kanegae, Y., Nucleic Acids Res., 23, 3816-3821(1995))。
MTAに書く付加的使用条件：
CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01675	pxCANCre	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pxCANCre was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01675).

Reference section:

Kanegae, Y., Takamori, K., Sato, Y., Lee, G., Nakai, M., Saito, I., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP]

Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14151_A6Ebp1-3.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 Sequence file: RDB14151_A6Ebc.seq
>01675_14151_A6Eb_3_pAxCA_F1_C08_08.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNCCT NCTTCTTTTN NCNACNGCTC CNGGGCAACG 61 TGCTGGTTAT TGTGCTGTCT CATCATTTTG GCAAAGAATT GATTTATCGA TTTGCAGACC 121 GTGCATCATG AGCGGCCCTC CAAAAAAGAA GAGAAAGGTA GAAGACCCGG GCGGCCGCAT 181 GTCCAATTTA CTGACCGTAC ACCAAAATTT GCCTGCATTA CCGGTCGATG CAACGAGTGA 241 TGAGGTTCGC AAGAACCTGA TGGACATGTT CAGGGATCGC CAGGCGTTTT CTGAGCATAC 301 CTGGAAAATG CTTCTGTCCG TTTGCCGGTC GTGGGCGGCA TGGTGCAAGT TGAATAACCG 361 GAAATGGTTT CCCGCAGAAC CTGAAGATGT TCGCGATTAT CTTCTATATC TTCAGGCGCG 421 CGGTCTGGCA GTAAAAACTA TCCAGCAACA TTTGGGCCAG CTAAACATGC TTCATCGTCG 481 GTCCGGGCTG CCACGACCAA GTGACAGCAA TGCTGTTTCA CTGGTTATGC GGCGGATCCG 541 AAAAGAAAAC GTTGATGCCG GTGAACGTGC AAAACAGGCT CTAGCGTTCG AACGCACTGA 601 TTTCGACCAG GTTCGTTCAC TCATGGAAAA TAGCGATCGC TGCCAGGATA TACGTAATCT 661 GGCATTTCTG GGGNNTGCTT ATAACACCCT GTTACGTATA GCCGAANTTG CCAGGNTCAG 721 GGTTAAAGAT ATCTCACGTA CTGACNGTGG GANAATGTTA ATCCATATTG GCAGAACGNN 781 NNNGCTGGTT ANCACCGCAN GNGTANANAA NGCACTTANC CTGGGGGTAA CTAAACNGNN 841 CGANCGANNG ANTTNCGNNN CTNGNNTANC TGANGNNNCN AATNNCTACC NNNTTTGCCN 901 GGNCANAAAA AAANGGNNNT GNCGNNNNNN CNNNCNNNNN NNNNN //
Primer: pAxCA_R1 Sequence file: RDB14151_A6Ebd.seq
>01675_14151_A6Eb_3_pAxCA_R1_F08_17.ab1 1 NNNNNNNNNN NNNNNNNNCN NNCNNNNNCC TTCTGNNAGG CAGCCTGCAC CTGNNGNNTG 61 AATTGATTAT CGATTCTAGA CTAGTTTAAT TAATTTCTAG AGCGCTTAAT GGCTAATCGC 121 CATCTTCCAG CAGGCGCACC ATTGCCCCTG TTTCACTATC CAGGTTACGG ATATAGTTCA 181 TGACAATATT TACATTGGTC CAGCCACCAG CTTGCATGAT CTCCGGTATT GAAACTCCAG 241 CGCGGGCCAT ATCTCGCGCG GCTCCGACAC GGGCACTGTG TCCAGACCAG GCCAGGTATC 301 TCTGACCAGA GTCATCCTTA GCGCCGTAAA TCAATCGATG AGTTGCTTCA AAAATCCCTT 361 CCAGGGCGCG AGTTGATAGC TGGCTGGTGG CAGATGGCGC GGCAACACCA TTTTTTCTGA 421 CCCGGCAAAA CAGGTAGTTA TTCGGATCAT CAGCTACACC AGAGACGGAA ATCCATCGCT 481 CGACCAGTTT AGTTACCCCC AGGCTAAGTG CCTTCTCTAC ACCTGCGGTG CTAACCAGCG 541 TTTTCGTTCT GCCAATATGG ATTAACATTC TCCCACCGTC AGTACGTGAG ATATCTTTAA 601 CCCTGATCCT GGCAATTTCG GCTATACGTA ACAGGGTGTT ATAAGCAATC CCCAGAAATG 661 CCAGATTACG TATATCCTGG CAGCGATCGC TATTTTCCAT GAGTGAACGA ACCTGGTCGA 721 AATCAGTGCG TTCGAACGCT AGANCCTGTT TTGCACGTTC ACCGGCATCA ACGTTTTTCT 781 TNTCNGATNC GCCGCATAAC CNNNNNAACA NCATTGCTGT CACTTGGNCG NNGNAGCCCN 841 GNNNNNNNAN NAANCATGTT TANCNNGCCC AANNNNNGCN NGANANTTTT NNNNGNNNNN 901 NNNNN //
Primer: RDB04134#1 Sequence file: RDB14151_A6Ebe.seq
>01675_14151_A6Eb_RDB04134#1_H04_22_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNGC NNNACTCTAA ACCNAATACT CATTCTGATG 61 TTTTAAATGA TTTGCCCTCC CNTATGTCCT TCCGAGTGAG AGACACAAAA AATTCCAACA 121 CACTATTGCA ATGAAAATAA ATTTCCTTTA TTAGCCAGAA GTCAGATGCT CAAGGGGCTT 181 CATGATGTCC CCATAATTTT TGGCAGAGGG AAAAAGATCT CAGTGGTATT TGTGAGCCAG 241 GGCATTGGCC ACACCAGCCA CCACCTTCTG ATAGGCAGCC TGCACCTGAG GAGTGAATTG 301 ATTATCGATT CTAGACTAGT TTAATTAATT TCTAGAGCGC TTAATGGCTA ATCGCCATCT 361 TCCAGCAGGC GCACCATTGC CCCTGTTTCA CTATCCAGGT TACGGATATA GTTCATGACA 421 ATATTTACAT TGGTCCAGCC ACCAGCTTGC ATGATCTCCG GTATTGAAAC TCCAGCGCGG 481 GCCATATCTC GCGCGGCTCC GACACGGGCA CTGTGTCCAG ACCAGGCCAG GTATCTCTGA 541 CCAGAGTCAT CCTTAGCGCC GTAAATCAAT CGATGAGTTG CTTCAAAAAT CCCTTCNAGG 601 GCGCGAGTTG ATAGCTGGCT GGTGGCAGAT GGCGCGGCAA CACCATTTTT TCTGACCCGG 661 CAAAACAGGT AGTTATTCGG ATCATCAGCT ACANCANAGA CGGAAATCCN TCGCTCGACC 721 AGTTTAGTTA NCCCCAGGCT NAGTGCCTTC TCTACNCCTG CNGNGCTAAC CAGCGTTTTC 781 GTTCTGCCNA TATGGATNAA CATTCTCCCN NCGTCAGNAC NNGANANATC TTNNANCCNN 841 GATCCNGNNN NNNTTCGGCT NNACNNNANN GGGNGTTATA AGCNNNNCNN NNNAAANGCN 901 NNANNNNNNA TATCCNNGNN NGCNNTCNNN NNTTTNNNNN NNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kanegae, Y., Efficient gene activation system on mammalian cell chromosomes using recombinant adenovirus producing Cre recombinase. Gene, 181, 207-212 (1996). PMID 8973332. [link to RRC of NBRP]
original	Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP]
reference	Matsuda, T., Controlled expression of transgenes introduced by in vivo electroporation. Proc. Natl. Acad. Sci. U.S.A. 104 (3): 1027-1032 (2007). PMID 17209010. [link to RRC of NBRP]
reference	Niwa, H., Efficient selection for high-expression transfectants with a novel eukaryotic vector. Gene, 108 (2): 193-200 (1991). PMID 1660837. [link to RRC of NBRP]
user_report	Nakahara, T., Activation of NF-κB by Human Papillomavirus 16 E1 Limits E1-Dependent Viral Replication through Degradation of E1. J. Virol. 89 (9): 5040-5059 (2015). PMID 25717108. [link to RRC of NBRP]
user_report	Sakai, Y., A novel transgenic mouse model carrying human Tribbles related protein 3 (TRB3) gene and its site specific phenotype. Biol. Pharm. Bull., 37 (6): 1068-1074 (2014). PMID 24882419. [link to RRC of NBRP]
user_report	Nodagashira T., Efficient Gene Transduction in HER2-Expressing Cells by Two Recombinant Adenovirus Vectors with HER2 Promotor and Cre/loxP System. Hirosaki Med. J., 61: 26-34 (2010). [link to RRC of NBRP]
user_report	Kameyama Y., An accumulative site-specific gene integration system using Cre recombinase-mediated cassette exchange. Biotechnol. Bioeng., 105, 1106-1114 (2010). PMID 19998287. [link to RRC of NBRP]
user_report	Marchetti G., Integrin alpha5beta1 is necessary for regulation of radial migration of cortical neurons during mouse brain development. Eur. J. Neurosci., 31, 399-409, (2010). PMID 20105241. [link to RRC of NBRP]
user_report	Komatsu, M., Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism. Proc. Natl. Acad. Sci. U S A., 107 (6), 2646-2651 (2010). PMID 20133795. [link to RRC of NBRP]
user_report	Shimada H., Introduction of tau mutation into cultured Rat1-R12 cells by gene targeting, using recombinant adeno-associated virus vector. Cell. Mol. Neurobiol., 29, 699-705 (2009). PMID 19305950. [link to RRC of NBRP]
user_report	Long N., Construction of a multi-functional helper-dependent adenovirus based system for cancer gene therapy. Asian Pac. J. Cancer Prev., 10, 939-960 (2009). PMID 20104994. [link to RRC of NBRP]
user_report	Pihkala P., An antigen-mediated selection system for mammalian cells that produce glycosylated single-chain Fv. Biochem. Biophys. Res. Commun., 324, 1165-1172 (2004). PMID 15504336. [link to RRC of NBRP]
user_report	Kurihara H., Glioma/glioblastoma-specific adenoviral gene expression using the nestin gene regulator. Gene Ther., 7, 686-693 (2000). PMID 10800092. [link to RRC of NBRP]