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pKM2L-phBMP4P2

Promoter Bank clone, Human bone morphogenetic protein 4 (BMP4), P2 promoter promoter

Catalog number RDB05473
Resource name pKM2L-phBMP4P2
Alternative name p1881-5F
Clone info. PCR amplified human bone morphogenetic protein 4 (BMP4), P2 promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Reference: Kawai, S., Bone 29 (1): 54-61 (2001), PMID 11472891; Shore, E.M., Calcif. Tissue Int. 63 (3): 221-229 (1998), PMID 9701626.
Comment The 2.2 kb genomic DNA corresponds to 3340 to 5582 nt of U43842.1 and 3339 to 5576 nt of AF210053.1, respectively. PCR cloning, forward primer, 1881F1: 5' atgggattcccgtccaagctatctc 3'; reverse primer, 1881R1: 5' cgagaaaacggtgaacagctttcgg 3'; Entire sequence of promoter region has not been confirmed. Promoter activity has not been analyzed.
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human bone morphogenetic protein 4 (BMP4), P2 promoter genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human bone morphogenetic protein 4 (BMP4), P2 promoter

          Reference sequence
            AF210053, U43842

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05473 pKM2L-phBMP4P2 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14630_A7Bsp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB14630_A7Bsa.seq check
          >05473_14630_A7Bs_M13(-40)_A08_01_ABI08.ab1
              1 CAGGGCCAGT CCAGCTTGCA TGCCTGCAGG TCATTTAAAT AGGGATAACA GGGTAATGAT
             61 TAAGATCTCG AGCTCTAAGC TTCACATATG CATGCACTAG TGGCATGGGA TTCCCGTCCA
            121 AGCTATCTCG AGCCTGCAGC GCCACAGTCC CCGGCCCTCG CCCAGGTTCA CTGCAACCGT
            181 TCAGAGGTCC CCAGGAGCTG CTGCTGGCGA GCCCGCTACT GCAGGGACCT ATGGTGAGCA
            241 AGGCTACCTG GTGAGGGGAG ACAGGCAGAG GGGGTCTAGG AGCCTCCTTG GGGGGAAGAA
            301 GCTGGTCACA GGCTGTGACC GAGGCAAAAG GTGGCCTAAT TATTTTCCAA TAGTGGTGCT
            361 GGAGGTGGGG ATGCTGGCGC TGAAAGACCT TTAAATATCG GCTACTGCCC CTGCCCAGGC
            421 CTTCTCTGTC CAGCAGTCCC TGGGAGATTC TCACCTTTGG GAAGTGCGGG GCAGGAGAGC
            481 AGAAACAAGA GAAGCCCTTG GTAGGGGGGT CGTTGGGAAA AACTGTGGGG TCTTGGGCTG
            541 AACGCGTTGC CCACGGGCTG GAGGTTGCGA TCCCCGGACG GAAAGCGCGG GAGGAGGAAG
            601 GAGAGAACCG GCTCTGAGGT CCAGAGAGAG TGAGGGGGCA GAGCGACGGC GAGATGGGGA
            661 GAGAACACCT AGCTGGAGCA GGTTCTGCGG TAGAGAGCGC AGTCCTGCTG GCCTCTGGAG
            721 AGTGCGCGCC GCTACGGAGG CTGCGTCGAG GGGAGTGTCA CCCAATCTGG CCCCCAGCTG
            781 GCGGGGCGCC CTGAGAGCTT GCGAACTGCA GTTGCAGGAC GCGCCTTCTC CACGAGCTAT
            841 TTTCGTCGAC TTGCGGAACC CAAGGAACCT CGCCTCTATC ATTTCACGGT GTAGGGTCCC
            901 TAGAGACGAC AGCCAAGATC CCAGGGGCTC CCAGGACGCT TGTTCCTGCG GTGTCGTGTC
            961 CTATGGGGAG TTCCTGGCGG ACGAAAGGCG GACGCGCGGC TCTTCCTGGC CCTCCAGGCC
           1021 CGGAACCGAC GGGAAAGGTT CCCGTGATTC CCGAGTCCCT GCAGCTTCTT CAGCGGAGTT
           1081 GTCGGGGGCT AGAGCTCAGC ACTGCTTGAG ATGTTTCAGA TCGCGGTTTG TGAGTGAGCT
           1141 TGTGAGAACT AACCTCAAAG AATACATTAC CTGTAACTGG AGGCCTCAAC CGTGTTGAAC
          //
          Primer: phRLR2
          Sequence file: RDB14630_A7Bsb.seq check
          >05473_14630_A7Bs_phRLR2_B07_02_ABI08.ab1
              1 AATTTTCCCT TTTCTGGAAA GCCCAGTTTC TATTGGTCTC CTTAAACCTG TCTTGTAACC
             61 TTGATACTTA CCTGCCCAGT GCCTCACGAC CAACTTCTGC AGTACCCGGG TGAATTCTAC
            121 GCGTCGACAG GCCGAGAAAA CGGTGAACAG CTTTCGGCCT GCGCTCGACC TCTGCGTCTG
            181 CGTCTCTCTC GCCTCGGCTT CCCTTATTTT TTAAACCACC ACCACACTCC TTCCCCCGCC
            241 ACTTCCTTCC CCCACCCCCT TCCTCCGTTG CACCAGCAGC AGAGTCGCAC GCAGCAAATA
            301 CTCCTTCAAG AATTTTACCT ACCTACAGTT CAAGCAGTTA CTGGGATGTC CTGACTAATC
            361 GAAGATGCTG CCGCGCGCGT GGGTCGCTCT GCGCAAGGGC CTCTTCGAAA ACCCGACTAG
            421 GCGCAACTCA GCGTTCAGCA GGGCCGGGAG CGCCAGGTCG TCCCCGGGGC CCGGGCCCCA
            481 TGACTCCTGC CCCAAAGCCC ACTCCACCCG ACCTCCCTTT CCTGAGGCTG TTCCCAGTTG
            541 CTGCTTTGGG TCGCTCCGGA GCTCAAGAAC TCGGGTTGCC TGCCGCCCCA CTCTCCACGC
            601 ACATACTTGG TTTTCTTCTT AGGGGCATTG GCAGGTAGAC TTTGAGGAAG AAAAGTAAAG
            661 GATCGAACAG CTCAGCCCTC CCTCCCGACC GTGGATGCCC GGAGTCGACC AACACCTCAG
            721 GTCCGGGTGC GGAGGCCGCG GGCGCCCCTG CGCGACCGTC CGCGCCCGGC AAGAGCCGCG
            781 CGGCTTTCGC CTTTGCTGGT CCCGCGCCAC CGCTGGGGCG GGCTGCGAAA GGGTTGGGAA
            841 GAGCAAAGGG TTTTTTTGTT TTGTTTTGAG ACGCAGAAGC CCTTTAAAAA GCCCGCCGAG
            901 GAGAGGTCCA GAAGTAGAGA AAGCAGACGG AGGCAAGCTG TGCCCGCGGG GCAAAGGGAC
            961 AGTAGAAGGG CGGGCGCCCG GGTTCCCCGG AAACCCTCGG CCCCAAGAAT CTCCTGGGCG
          1021 GGAGAGCGCG GTTCTAAACC GAGAGGATAG GAAGGGAGGG GGGAGTTGGT GTTTCAAT
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB14630_A7Bsc.seq check
          >05473_14630_A7Bs_pAxCALNL_F1_C07_03_ABI08.ab1
              1 AACCAGGTTT TCTTATCATG TCTGCTCGAA GCGGCCGCTC TAGGAATTAC TGCTCGTTCT
             61 TCAGCACGCG CTCCACGAAG CTCTTGATGT ACTTACCCAT TTCATCTGGA GCGTCCTCCT
            121 GGCTGAAGTG GAGGCCCTTC ACCTTCACGA ACTCGGTGTT AGGGAACTTC TTAGCTCCCT
            181 CGACAATAGC GTTGGAAAAG AACCCAGGGT CGGACTCGAT GAACATCTTA GGCAGATCGT
            241 CGCTGGCCCG AAGGTAGGCG TTGTAGTTGC GGACAATCTG GACGACGTCG GGCTTGCCTC
            301 CCTTAACGAG AGGGATCTCG CGAGGCCAGG AGAGGGTAGG CCGTCTAACC TCGCCCTTCT
            361 CCTTGAATGG CTCCAGGTAG GCAGCGAACT CCTCAGGCTC CAGTTTCCGC ATGATCTTGC
            421 TTGGGAGCAT GGTCTCGACG AAGAAGTTAT TCTCAAGCAC CATTTTCTCG CCCTCTTCGC
            481 TCTTGATCAG GGCGATATCC TCCTCGATGT CAGGCCACTC GTCCCAGGAC TCGATCACGT
            541 CCACGACACT CTCAGCATGG ACGATGGCCT TGATCTTGTC TTGGTGCTCG TAGGAGTAGT
            601 GAAAGGCCAG ACAAGCCCCC CAGTCGTGGC CCACAAAGAT GATTTTCTTT GGAAGGTTCA
            661 GCAGCTCGAA CCAAGCGGTG AGGTACTTGT AGTGATCCAG GAGGCGATAT GAGCCATTCC
            721 CGCTCTTGCC GGACTTACCC ATTCCGATCA GATCAGGGAT GATGCATCTA GCCACGGGCT
            781 CGATGTGAGG CACGACGTGC CTCCACAGGT AGCTGGAGGC AGCGTTACCA TGCAGAAAAA
            841 TCACGGCGTT CTCGGCGTGC TTCTCGGAAT CATAGTAGTT GATGAAGGAG TCCAGCACGT
            901 TCATTTGCTT GCAGCGAGCC CACCACTGAG GCCCAGTGAT CATGCGTTTG CGTTGCTCGG
            961 GGTCGTACAC CTTGGAAGCC ATGGTGGCTA GCCTATAGTG AGTCGTATTA AGTACTCTAG
           1021 CCTTAAGAGC TGTAATTGAA CTGGAGTGGA CACTGTGGAG AGAAAGGCAA AGTGATGTCA
           1081 GTAGACCATA GGTGCCTATC AGAAACCGCA GAGTCTTCTC TGTCTCGACA GCCCAGGTTT
           1141 CTATTGGTCT CTAACCTGTC TGTAACCTTG ATACTAACCT GCAAGTGCCT ACGACACTCC
           1201 TGCCAGTACC TGGTGATCTA CCGTCGACGG CCGAGAAAAC GGGTGACACA GCCTTTCCGG
          1261 C
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          reference Kawai, S., Characterization of human bone morphogenetic protein (BMP)-4 and -7 gene promoters: activation of BMP promoters by Gli, a sonic hedgehog mediator. Bone 29 (1): 54-61 (2001). PMID 11472891.
          reference Shore, E.M., The human bone morphogenetic protein 4 (BMP-4) gene: molecular structure and transcriptional regulation. Calcif. Tissue Int. 63 (3): 221-229 (1998). PMID 9701626.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl