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pKM2L-phMBP

Promoter Bank clone, Human myelin basic protein (MBP) promoter

Catalog number RDB05890
Resource name pKM2L-phMBP
Alternative name p1973-12F
Clone info. PCR amplified human myelin basic protein (MBP) promoter sequence was inserted into a Renilla luciferase reporter vector pKM2L (RDB04026). Cloned fragment: 77019999 to 77017024(NC_000018.10); relatively -2944 to +32, where +1 corresponds to 1 nt of NM_001025081.2.
Comment PCR cloning, forward primer, 1973 F2: 5' aggctgtgatacccctgctgttgct 3'; reverse primer, 1973 R2: 5' ggaggtggctgcactgtcttcttgg 3' Entire sequence of promoter region has not been confirmed. (reference: Brain Res. Dev. Brain Res., 65, 217-221, 1992, PMID:1373994; Gene, 313, 161-167, 2003, PMID:12957387; Gene, 75, 31-38, 1989, PMID:2470651)
Vector backbone pKM2L (RDB04026) (Plasmid)
Size of vector backbone 4.2 kb
Selectable markers Kan^r
Gene/insert name Human myelin basic protein (MBP) genomic DNA
Depositor|Developer DNA Bank, |
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External Database
Human myelin basic protein (MBP)

          Reference sequence
            AC093330

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Additional terms and conditions for distribution The use of the BIOLOGICAL RESOURCE is restricted to the academic researches conducted by non-profit organization. By using this material, the RECIPIENT agrees to be bound by the conditions of the limited use statement of the Promega Corporation.
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          RDB05890 pKM2L-phMBP DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Promoter Collection (Renilla Luciferase) (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13698_A5Jsp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB13698_A5Jsa.seq check
          >05890_13698_A5Js_M13(-40)_D11_11.ab1
              1 CCGGCAGCAG CTTGCATGCC TGCAGGTCAT TTAAATAGGG ATAACAGGGT AATGATTAAG
             61 ATCTCGAGCT CTAAGCTTCA CATATGCATG CACTAGTGGC AGGCTGTGAT ACCCCTGCTG
            121 TTGCTCACAG ATGGTCAGTT CCCTGCACAG GCCCTCCTGC CTCCCAGGTT ACTGCCTGGC
            181 CTGGGAGGTT CTCAGCCCGG CCATCCTCCT GGACCCAGAA CCAGCATGGC CGCTCTCCTG
            241 CTGCTTCCGC CACCGGGAGC CGATTCCGCG CAGGCTTCCA GTGGGGGCTG CCTTCCCCTC
            301 TGCCAGAAGC ACCCTCCCCA ACTTCCCCAA GTCCTCGCAG GGCCCAGACT CTCATCTTGT
            361 CCTGTCATTT CCGCAAGGAC CTTCCTGCTG ACCCAAGCCC TCTCTGCTTT CCTATTCTCT
            421 TGCCTGATGT GAAGTTGCAC ATTGCATGAG TTGGGGCTGC CAAGACAAAT TACCATATGC
            481 TGGGGGCTTC AAACTACAGG AATTCTTCTG TGACCATTGT GAAGGCCAGA AAGCTCAAGT
            541 TGAGATGTCT GCAGAGCCCT GCTTCCCCCG AAGGCTCTAC AGGAGAATTT GCTCCATGTC
            601 TCTTACTCCT GGTGGTGCTC AGAAGTGCTT GGTGTTCTGA GGTTTGTAGA TGCCCCCCTC
            661 CAGGCTCCTC CTCCATCTTC ACACCTCTCC CCTGTGCATC TGTGGCTGTG TCCAAACTGC
            721 TCTCTTCTTA CAAGGACACC AGTGATTGGA GTAGGGCCCA CCCTACTGCA GAATGACCTC
            781 ATCTTCACTT GATTCCACCT GCAAAGATCT ATTTCCCAAA TAAAGGTCAC ACTCACAGGT
            841 ACAGAAGTTA GGACATGGGA CATATCATTT GGAGGGATAC TATTTCAATC CCATAACATA
            901 CATTTTCACC TGCTTTCCCC CACTAGAATG TAGTTTCACG AGGACAAGAC CTCGTGAGTA
            961 CATCACTGTA CTCAGAATAT TTAGATGTAC ATTACATTAA TTTGTACTCA TAATTATTGG
          1021 ATTGGATTGG ATTCATTGGT ATGTATGGTA TGTAACCAGG TACGCTTAAT TAGATTGG
          //
          Primer: phRLR2
          Sequence file: RDB13698_A5Jsb.seq check
          >05890_13698_A5Js_phRLR2_E11_14.ab1
              1 ACTCCCGGTC TCGAAGCCCA GTTTCTATTG GTCTCCTTAA ACCTGTCTTG TAACCTTGAT
             61 ACTTACCTGC CCAGTGCCTC ACGACCAACT TCTGCAGTAC CCGGGTGAAT TCTACGCGTC
            121 GACAGGCGGA GGTGGCTGCA CTGTCTTCTT GGATGGTCTG GAGCTCGTCG GACTCAGAGG
            181 GCCTGTCTTT GAAGGTGTTG TCCTCCCTCC CCGGGGCATC TCGGGAAAAG AGGCGGATCA
            241 AGTGGGGGCG GCTCCCTGGG TCAGCTGGGT GGGCATCCTG CCAGGCATTC TTCGGGTCCG
            301 CTGTGCGCTT GGAGTCAGTC ACCGCTGTGT CCTGAGAGGA GGTCCCATTG TTCTGGTTCG
            361 CATCTGCCTC TCCTGCAAAC AACAATGAGA TATGAATACG GGCCTGTGCA AAGCTGAGCA
            421 CCGGACAAAG CAGCTTTCTC TGAGGGGTCT TGACCTAGCT GTCTCCTATA AAACCCACAG
            481 AATATGTGCA GTCCTCATAA AGCCCTTGGC CTAGGATGGA GCTAACCTGG ATGGAGCAGC
            541 CTTATTGCAG CTCTGTGTTT CAGAAGGCTC TGGGAATTCC TCAGTTACTC GAGATTTGGC
            601 ACCCAGCGTA AAATGCACCC AGGCTTCCCG ATGAAGTCAT CTGAGCATGC AGAGCAGCCA
            661 CATCAGCGTT TCTTCCCGGG TGCTGCCATG GCTGCTCCCG GAGAGCCCAG ATGTGCAGGC
            721 ACCTTGATGG GACATTGACT GTTTAAAAGG CCACTGGTGC ATAAGTTAAA TATGCAAGGA
            781 GCATTTATTT GACAAAAGGT GTTTTTAGTT TTCTTGGTAG AAACAAAACT TGCCACATCT
            841 TTTTGCAATT CATGCATGAA AGAAGTGGGG GCATTTAGAA TTGTTTCAAT CCCAGATTCC
            901 ACAAGAGGCC TCTCACAATA GAGAACTGCT TATTCAGGAC ATTTTGACTT GGCTTCTCCT
            961 CTGGGAAAGC AGAAACAAAT TAGAAATCTG AAAGTGGGAT TGAGATATTT CATGTTTGTA
           1021 TACCCAGCAT CTCATAGCTT TTCCTCAAAA AAATACCACA TTTTCTTGTC ACGAAGGCCA
          1081 ATTGCAACTC GATTTTGGCC TATCTAGTAC GAGTGATCAC GCATTTCAGA TCAT
          //
          Primer: pAxCALNL_F1
          Sequence file: RDB13698_A5Jsc.seq check
          >05890_13698_A5Js_pAxCALNL_F1_F11_17.ab1
              1 ACATTATCTT ATCATGTCTG CTCGAGCGGC CGCTCTAGAA TTACTGCTCG TTCTTCAGCA
             61 CGCGCTCCAC GAAGCTCTTG ATGTACTTAC CCATTTCATC TGGAGCGTCC TCCTGGCTGA
            121 AGTGGAGGCC CTTCACCTTC ACGAACTCGG TGTTAGGGAA CTTCTTAGCT CCCTCGACAA
            181 TAGCGTTGGA AAAGAACCCA GGGTCGGACT CGATGAACAT CTTAGGCAGA TCGTCGCTGG
            241 CCCGAAGGTA GGCGTTGTAG TTGCGGACAA TCTGGACGAC GTCGGGCTTG CCTCCCTTAA
            301 CGAGAGGGAT CTCGCGAGGC CAGGAGAGGG TAGGCCGTCT AACCTCGCCC TTCTCCTTGA
            361 ATGGCTCCAG GTAGGCAGCG AACTCCTCAG GCTCCAGTTT CCGCATGATC TTGCTTGGGA
            421 GCATGGTCTC GACGAAGAAG TTATTCTCAA GCACCATTTT CTCGCCCTCT TCGCTCTTGA
            481 TCAGGGCGAT ATCCTCCTCG ATGTCAGGCC ACTCGTCCCA GGACTCGATC ACGTCCACGA
            541 CACTCTCAGC ATGGACGATG GCCTTGATCT TGTCTTGGTG CTCGTAGGAG TAGTGAAAGG
            601 CCAGACAAGC CCCCCAGTCG TGGCCCACAA AGATGATTTT CTTTGGAAGG TTCAGCAGCT
            661 CGAACCAAGC GGTGAGGTAC TTGTAGTGAT CCAGGAGGCG ATATGAGCCA TTCCCGCTCT
            721 TGCCGGACTT ACCCATTCCG ATCAGATCAG GGATGATGCA TCTAGCCACG GGCTCGATGT
            781 GAGGCACGAC GTGCCTCCAC AGGTAGCTGG AGGCAGCGTT ACCATGCAGA AAAATCACGG
            841 CGTTCTCGGC GTGCTTCTCG GAATCATAGT AGTTGATGAA GGGAGTCCAG CACGTTTCAT
            901 TTGCTTGCAG CGAGCCTCAC TCACTGAGGC CCAGTGATCA TGCGTTTGCG TTTGCTCGGG
            961 GTCGTACACC TTTGGAAGCC CATGGGTGGC TAGCTATAGT GAGTCGTATT AAGTACTCTA
           1021 GCTTAGAGCT GTAATTGACC TGGGGAGTGG ACACCCTGTG GCAGAGAAAG CTAAGGTTGC
          1081 AATGTTCAGG TAAGGACTCA T
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles


          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl