Resource data sheet
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pRSFlag_mmSumo1 (#RDB06126)

Expression vector of mouse Sumo1 coding sequence

Alternative name SET-0156_4
Clone info. Expression vector of mouse Sumo1 coding sequence. Expression was confirmed by Western blotting with anti-FLAG antibody.
Vector backbone pRSV_s-FLAG (RDB06083) (Plasmid)
Size of vector backbone 5.19 kb
Selectable markers Amp^r
Gene/insert name Mouse Sumo1 cDNA
Depositor|Developer DNA Bank, | Murata, Takehide |
 
Sequence RDB06126z.seq

Distribution information

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Terms and conditions for distribution 1. The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. 2. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB06126 pRSFlag_mmSumo1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pRSFlag_mmSumo1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB06126).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13461_A5G7.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: RSVpro_F
Sequence file: RDB13461_A5G7a.seq check
>06126_13461_A5G7_RSVpro_F_A10_01.ab1
    1 AAGTTTGACA TTCACCACAT TGGTGTGCAC CTCCAAGCTG AATTCACCAT GGACTACAAG
   61 GACGATGACG ATAAGGCGGC CATGTCTGAC CAGGAGGCAA AACCTTCAAC TGAGGACTTA
  121 GGCGATAAGA AGGAAGGAGA ATACATTAAA CTCAAAGTTA TTGGACAGGA TAGCAGTGAG
  181 ATACATTTCA AAGTGAAAAT GACAACACAT CTCAAGAAAC TCAAAGAATC ATACTGTCAA
  241 AGACAGGGAG TTCCAATGAA TTCACTCAGG TTTCTCTTTG AAGGTCAGAG AATTGCTGAT
  301 AATCATACTC CGAAAGAACT GGGAATGGAG GAAGAAGATG TGATTGAAGT TTATCAGGAA
  361 CAAACGGGGG GTCACTCGAC GGTTTAGGGC CGCTCGAGCA TGCATCTAGA GCTCGCTGAT
  421 CAGCCTCGAC TGTGCCTTCT AGTTGCCAGC CATCTGTTGT TTGCCCCTCC CCCGTGCCTT
  481 CCTTGACCCT GGAAGGTGCC ACTCCCACTG TCCTTTCCTA ATAAAATGAG GAAATTGCAT
  541 CGCATTGTCT GAGTAGGTGT CATTCTATTC TGGGGGGTGG GGTGGGGCAG GACAGCAAGG
  601 GGGAGGGTTG GGAAGACAAT AGCAGGCATG CTGGGGATGC GGTGGGCTCT ATGGCTTCTG
  661 AGGCGGAAAG AACCAGCTGG GGCTCGAGGG GGGATCCCCA CGCGCCCTGT AGCGGCGCAT
  721 TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG
  781 CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGGT
  841 CAAGCTCTAA ATCGGGGGGC TCCCCTTTAG GGGTTCCGAT TTTAGTGCTT TAACGGCACC
  901 TCGACCCCAA AAAACTTGAT TAGGGGTGAT GGTTTCACGT TATTGGGCCA TCGCCCCTGA
961 TAAACGGTTT TTTCACCCTT TGACGTGGAA GTTCCACCGT TCTTTTAAAA ATGGA
//
Primer: SV40pro_F_V2
Sequence file: RDB13461_A5G7b.seq check
>06126_13461_A5G7_SV40proF_V2_B10_04.ab1
    1 TGGCCCGTTT TTGGAGCCTA GGCTTTTGCA AAAGCTCCCG GGAGCTTGGA TATCCATTTT
   61 CGGATCTGAT CAAGAGGCAG GATGAGGATC GTTTCGCATG ATTGAACAAG ATGGATTGCA
  121 CGCAGGTTCT CCGGCCGCTT GGGTGGAGAG GCTATTCGGC TATGACTGGG CACAACAGAC
  181 AATCGGCTGC TCTGATGCCG CCGTGTTCCG GCTGTCAGCG CAGGGGCGCC CGGTTCTTTT
  241 TGTCAAGACC GACCTGTCCG GTGCCCTGAA TGAACTGCAG GACGAGGCAG CGCGGCTATC
  301 GTGGCTGGCC ACGACGGGCG TTCCTTGCGC AGCTGTGCTC GACGTTGTCA CTGAAGCGGG
  361 AAGGGACTGG CTGCTATTGG GCGAAGTGCC GGGGCAGGAT CTCCTGTCAT CTCACCTTGC
  421 TCCTGCCGAG AAAGTATCCA TCATGGCTGA TGCAATGCGG CGGCTGCATA CGCTTGATCC
  481 GGCTACCTGC CCATTCGACC ACCAAGCGAA ACATCGCATC GAGCGAGCAC GTACTCGGAT
  541 GGAAGCCGGT CTTGTCGATC AGGATGATCT GGACGAAGAG CATCAGGGGC TCGCGCCAGC
  601 CGAACTGTTC GCCAGGCTCA AGGCGCGCAT GCCCGACGGC GAGGATCTCG TCGTGACCCA
  661 TGGCGATGCC TGCTTGCCGA ATATCATGGT GGAAAATGGC CGCTTTTCTG GATTCATCGA
  721 CTGTGGCCGG CTGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA CCCGTGATAT
  781 TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG GTATCGCCGC
  841 TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT GAGCGGGACT
  901 CTGGGGTTCG AAATGACCGA CCAAGCGACG CCCAACCTGC CATCACGAGA TTTCGATTCC
  961 ACCGCCGCCT TCTATGAAAG GTTGGGGCTT CGGAATCGTT TTCCGGGACG CCCGGCTGGA
 1021 ATGATCCTTC CAGCGCGGGG ATCTCATGCT GGAGTTTCTT CGCCCACCCC AACTTTGTTA
 1081 TTGCAGCCTT ATATGGGTTA ACAAATTAAA GCATAGGCAA TTCACCAAAA ATTTTTCCAC
1141 C
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Gur, I., Necdin promotes ubiquitin-dependent degradation of PIAS1 SUMO E3 ligase. PLoS One, 9 (6): e99503 (2014). PMID 24911587. [link to RRC of NBRP]