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pcDNA3.1-His-Gli1 (#RDB08064)

Expression vector of mouse Gli1 cDNA

Clone info. Expression vector of mouse Gli1 cDNA.
Vector backbone pcDNA3.1/HisB (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r
Gene/insert name Mouse Gli1 cDNA
Depositor|Developer Sasaki, Hiroshi |
 

Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested. (Development, 126, 3915-3924 (1999).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. The use of the BIOLOGICAL RESOURCE is restricted to the scientific researches conducted by non-profit organization.
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Development, 126, 3915-3924 (1999).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。本件リソースの利用は、非営利機関による学術研究に限る。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB08064 pcDNA3.1-His-Gli1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3.1-His-Gli1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB08064).

Reference section:

Sasaki, H., Nishizaki, Y., Hui, C., Nakafuku, M., Kondoh, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17503_A9Ibp1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: CMV pro, 6xHis, T7 tag, Xpress tag, insert 5'
Sequence file: RDB17503_A9Ibd.seq check
>08064_17503_A9Ib_3_CMV_Forward_A02_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT NNNNGCANTG GGCGGTAGGC GTGTACGGNG GGANGTCTAT
   61 ATAAGCAGAG CTCTCTGGCT AACTAGAGAA CCCACTGCTT ACTGGCTTAT CGAAATTAAT
  121 ACGACTCACT ATAGGGAGAC CCAAGCTGGC TAGCGTTTAA ACTTAAGCTT ACCATGGGGG
  181 GTTCTCATCA TCATCATCAT CATGGTATGG CTAGCATGAC TGGTGGACAG CAAATGGGTC
  241 GGGATCTGTA CGACGATGAC GATAAGGTAC CTAAGGATCC AGTGTGGTGG AATTCTGCAG
  301 ATGACGCCAT GTTCAATCCA ATGACTCCAC CACAAGTCAA TAGCTATAGT GAGCCATGCT
  361 GTCTCCGACC CCTCCACAGC CAAGGAGTCC CCAGCATGGG AACAGAAGGA CTTTCTGGTC
  421 TGCCCTTTTG CCACCAAGCC AACTTTATGT CAGGGTCCCA GGGTTATGGA GCAGCCAGAG
  481 AGACCAGCAG CTGCACTGAA GGATCTCTCT TTCCTCCTCC TCCTCCTCCT CGGAGTTCAG
  541 TCAAATTAAC AAAGAAGCGG GCTCTCTCCA TCTCGCCCCT TTCTGATGCC AGCCTCGACC
  601 TGCAAACCGT AATCCGGACC TCACCCAGCT CCCTGGTGGC TTTCATCAAC TCTCGCTGTA
  661 CATCTCCGGG CGGTTCCTAC GGCCATCTCT CCATTGGTAC CATGAGCCCT TCTTTAGGAT
  721 TCCCACCTCA GATGAGTCAT CAAAAAGGAA CTTCACCTCC CTATGGAGTC CAGCCCTGTG
  781 TTCCACATGA CTCTACTCGG GGTTCAATGA TGCTTCACCC CNAGGCCCGG GGANCACGTG
  841 CAACCTGCCA GCTGAAGTCA GANCTGGATA TGATGNTTGG NAAGTGCCCG GANGNNCCNT
  901 TNGANNGGGA NATGNCTAGC CCCAACNNNN CANGNNTACA NGATCACCTG TNGGGGANGC
961 TGGNNGGNNN GNANGNNCNN NNNNNANANG ANAANNCCNN ANCNNNAN
//
Primer: bGH-B_R (Pr0746)
Region: insert 3'
Sequence file: RDB17503_A9Ibe.seq check
>08064_17503_A9Ib_3_bGH-B_R_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNNGTNNNNN NGGCCCTCTA GANTCGAGCG
   61 GCCGCTTAGG CACTAGAGTT GAGGAATTGT GTCTCTCCAG GCAGAGACCC CAGCAAGACA
  121 CTCATGTTAC CCACTGCCAT GTTGGGGGGC CCAGAGGGAG ATGGGGTGTT TTTAGAGCTG
  181 TCCCCTTGCT CATGGGAAAG AGGAGGGCTC AGGCCCTGGG CCTCATCTAG GATAGCCACA
  241 AAGTCCAGCT GAGTGTTGTC CAGGTCAAGA GAGTCCAGAG CGTTACACAC CTGCCCTTCA
  301 GGAGGAGGGT ACAAGGCAGG GCCTGCTCCT AACCTGCCCA CCTCAGGATG GCCACAGCTG
  361 GGGTTGGTAT CCCCCACCTT GAGGGGCCCA TAGCAAGGGG ACAGCGGGGG CAGAAGTCGG
  421 GGTGGTGCTG CTGGCCTGTG GGAAGGCCTG TTTACTCCCA CGGTGAAAGT TTCATGGCAG
  481 GGTGAAGGGG CAGGATAGGA GCCTGATTTG TGATTGGCCG AAGCAGGTGC AAAGCCAGAT
  541 CCATATGCTG CCGCCGCTGC TGCTGGGGTC TTGGCAGGGC TCTGACTAAC TTGGGAACCC
  601 CCCANAAACT TGGGGCTCTG GTATGGGAGT TCCTGGTTGG GGAGCCCTCC CCGGTTTCTT
  661 CCCTCCCACA ACAATTCCTG CTGCGACTGA ACGTAATTAC ACACCAGCTG AGCTTTGAGC
  721 TGTCCTGTGG AATGAGAGGA GGAGGGGTTG AAATTGANGC CAAGCCTGGG TTCTGTTGAG
  781 AGATAACCAT GGGGANGCTG ANGATAGGGA NCCGACTGGG GNTACTGNNG CTGANGGANC
  841 TGGGGATGAT GTGAAAACAG ANGCTGCNGG NCCTGNNNAN CCTTCNCTGG GGNGGGCATT
901 NNNNNNNNNN
//
Primer: SV40pro_ori_F (Pr0731)
Region: SV40pro_ori, NeoR
Sequence file: RDB17503_A9Ibf.seq check
>08064_17503_A9Ib_3_SV40pro_ori_F_C02_03_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNCCTCTG CCTCTGANCN NTTCNNGAAG TAGTGAGGAG
   61 GCTTTTTTGG AGGCCTAGGC TTTTGCAAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
  121 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  181 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  241 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  301 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  361 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  421 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  481 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  541 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  601 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  661 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA NGATCTCGTC GTGACCCATG
  721 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  781 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
  841 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCNT GCTTTACGGN ATCNNNGCTC
  901 CNGATTCGCA GNGCATCNCC TTCTATCNNN TTCTNGACNA NTNCTTCTGA NCGGGNNTCN
  961 GGGGNNTCGA AANTGANCGA NCNANNNNNN NCCNANCNTG NCNNNCNNNN NNNNTNNNAN
1021 TNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Sasaki, H., Regulation of Gli2 and Gli3 activities by an amino-terminal repression domain: implication of Gli2 and Gli3 as primary mediators of Shh signaling. Development, 126, 3915-3924 (1999). PMID 10433919. [link to RRC of NBRP]
user_report Shiohama, T., Oxidative stress-induced JNK1 phosphorylation inhibits hedgehog signalling and osteoblast differentiation. Cell Biol. Int. Rep. 21 (2); 53-62 (2014). [link to RRC of NBRP]