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pMAK17 (#RDB09002)

Expression clone of EGFP under the control of tet inducible promoter. Puromycin serectable

Clone info. Expression clone of EGFP under the control of tet inducible promoter. Puromycin serectable.
Vector backbone pMAK42 (Plasmid)
Size of vector backbone 9.2 kb
Selectable markers Amp^r
Gene/insert name Aequorea victoria GFP cDNA
Depositor|Developer Iwaki, Takayuki |
 
Sequence (full) RDB09002zzk01.seq provided by the depositor

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature designated by the DEPOSITOR is requested (Cytotechnology, 63(4), 337-343 (2011)).
Remarks An additional license fee for DNA resources containing fluorescent proteins owned by, or licensed to, Clontech Laboratories, Inc. is required.
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MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Cytotechnology, 63(4), 337-343 (2011))。
備考 クロンテック社の蛍光タンパク質 (DsRed2、mCherry)を含むクローン。ライセンス料相当額を負担して頂きます。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB09002 pMAK17 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge plus licensing fee equivalent for fluorescent protein

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pMAK17 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB09002).

Reference section:

Iwaki, T., Umemura, K., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB09002_A3Fb.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_22mer
Sequence file: RDB09002_A3Fba.seq check
>09002_09002_A3Fb_T7_22mer_C06_09.ab1
    1 TGGCGGAAGC AGTGAAAATG CTTTATTTGT GAATTTGTGA TGCTATTGCT TTATTTGTAA
   61 CCATTATAAG CTGCAATAAA CAAGTTAACA ACAACAATTG CATTCATTTT ATGTTTCAGG
  121 TTCAGGGGGA GGTGTGGGAG GTTTTTTAAA GCAAGTAAAA CCTCTACAAA TGTGGTATGG
  181 CTGATTATGA TCCTCTAGAG ATATCGTCGA CAAGCTTATC GATGCGGCCT TACTTGTACA
  241 GCTCGTCCAT GCCGAGAGTG ATCCCGGCGG CGGTCACGAA CTCCAGCAGG ACCATGTGAT
  301 CGCGCTTCTC GTTGGGGTCT TTGCTCAGGG CGGACTGGGT GCTCAGGTAG TGGTTGTCGG
  361 GCAGCAGCAC GGGGCCGTCG CCGATGGGGG TGTTCTGCTG GTAGTGGTCG GCGAGCTGCA
  421 CGCTGCCGTC CTCGATGTTG TGGCGGATCT TGAAGTTCAC CTTGATGCCG TTCTTCTGCT
  481 TGTCGGCCAT GATATAGACG TTGTGGCTGT TGTAGTTGTA CTCCAGCTTG TGCCCCAGGA
  541 TGTTGCCGTC CTCCTTGAAG TCGATGCCCT TCAGCTCGAT GCGGTTCACC AGGGTGTCGC
  601 CCTCGAACTT CACCTCGGCG CGGGTCTTGT AGTTGCCGTC GTCCTTGAAG AAGATGGTGC
  661 GCTCCTGGAC GTAGCCTTCG GGCATGGCGG ACTTGAAGAA GTCGTGCTGC TTCATGTGGT
  721 CGGGGTAGCG GCTGAAGCAC TGCACGCCGT AGTCAGGTGG TCACGAGGGT GGGCCAGGGC
  781 ACGGGCAGCT TGCCGGTGGT GCAGATGAAC TTTCAGGGTC AGCTTGCCGT AGTGGCATCG
  841 CCCTCGGCCC TCCGCCGACA CGCTGAACTT GTGGCCGTTA CGTCGCCGTC AGCTCGACAG
  901 GATGGCACCA CCCCGTGACA GCTCTCGCCC TGCTCACCAT GGTGGCGGCG ATCCGGGTAC
  961 GAGCTCGATC CAAGGCGAAT TGACGTCCAT ACGGATCTTG CTTAATAGCT CACGTTACGG
1021 CCTACCTGCA ATCGTCCTAT AATGATGGAA TAATC
//
Primer: SV40pA3'Element Reverse
Sequence file: RDB09002_A3Fbb.seq check
>09002_09002_A3Fb_SV40pA3'Element Reverse._D06_12.ab1
    1 GGAGTCAAGG GGGGCGGAGC TATGGAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG
   61 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT
  121 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC
  181 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG
  241 CGTTGGCCGA TTCATTAATG CAGCTGGCAC GACAGGTTTC CCGACTGGAA AGCGGGCAGT
  301 GAGCGCAACG CAATTAATGT GAGTTAGCTC ACTCATTAGG CACCCCAGGC TTTACACTTT
  361 ATGCTTCCGG CTCGTATGTT GTGTGGAATT GTGAGCGGAT AACAATTTCA CACAGGAAAC
  421 AGCTATGACC ATGATTACGC CAAGCGCGCA ATTAACCCTC ACTAAAGGGA ACAAAAGCTG
  481 GAGCTCACCC GATCCAGACA TGATAAGATA CATTGATGAG TTTGGACAAA CCACAACTAG
  541 AATGCAGTGA AAAAAATGCT TTATTTGTGA AATTTGTGAT GCTATTGCTT TATTTGTAAC
  601 CATTATAAGC TGCAATAAAC AAGTTCAAGC TGCGGCCGCC GTATCGAGGT CGACGGTATC
  661 GATAAGCTTG ATCTAGAGTC GGTGGGCCTC CTACAGGAAC AGGTGGTGGC GGCCCTCGGT
  721 GCGCTCGTAC TGCTCCACGA TGGTGTAGTC CTCGTTGTGG GAGGTGATGT CCAGCTTGGC
  781 GTCCACGTAG TAGTAGCCGG GCAGCTGCAC GGGCTTCTTG GCCATGTAGA TGGACTTGAA
  841 CTCCACCAGG TAGTGGCCGC CGTCCTTCAG CTTCAGGGCC TTGTGGGTCT CGCCCTTCAG
  901 CACGCCGTCG CGGGGGGTAC AGGCGCTCGG TGGAGGCCCT CCCAGCCCAT GGTCTTCTTC
  961 TGCATCACGG GGCCGTCGGA GGGGAAAGTT CACGCCCGAT GAACTTCACC TTGTAGATGA
 1021 GCAGCCGTCC TGCAGGGAGA GTCCTGGGGT CACGGGTCGC CACCGCCGCG GTTCTCGAGT
 1081 CCATCACGCG CTCACTGGAG TCCTCGGGCA GACAGCTCTT GATCGGATGT CCGATCGGAA
1141 GACCTCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Iwaki, T., A single plasmid transfection that offers a significant advantage associated with puromycin selection, fluorescence-assisted cell sorting, and doxycycline-inducible protein expression in mammalian cells. Cytotechnol, 63, 337-343 (2011). PMID 21553115. [link to RRC of NBRP]