RDB14128 - pDll1-Fluc

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pDll1-Fluc (#RDB14128)

Reporter vector for time-lapse imaging of Dll1 protein.

Clone info.	Reporter vector for time-lapse imaging of Dll1 protein. This construct contains Dll1 promoter, Dll1 gene and luciferase cDNA.
Comment	Expression was confirmed by the depositor with bioluminescence imaging. See Fig.S4 of Shimojo, H. et al., Genes Dev. 30 (1): 102-116, 2016.
Vector backbone	pBluescript (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	Mouse Dll1 genomic DNA Mouse Dll1 genomic DNA firefly (Photinus pyralis) cDNA
Depositor\|Developer	Kageyama, Ryoichiro \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shimojo, H. et al., Genes Dev. 30(1):102-116, 2016)

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. (Shimojo, H. et al., Genes Dev. 30(1):102-116, 2016)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Shimojo, H. et al., Genes Dev. 30(1):102-116, 2016)。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB14128	pDll1-Fluc	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pDll1-Fluc was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB14128).

Reference section:

Shimojo, H., Isomura, A., Ohtsuka, T., Kori, H., Miyachi, H., Kageyama, R., Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30 (1): 102-116 (2016). PMID 26728556. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB14128_A6Djp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 Sequence file: RDB14128_A6Dja.seq
>D03343A2_A6Dj_1_M13_-40_A11_02.ab1 1 TTTGCCGAAG GGAAATGGTA ATACGACTCA CTATAGGGCG AATTGGGGTA CCGGGGCCCC 61 CCCTCGAGGT CGACGGTATC GATTGATACC AGGTGAGGTG AGAGCACTGT GGCCCGGGCC 121 CAGCTGCATG GAACCAGCAC GACTTCGTCT CAAAGCTTTG TGACTCAAGT CGTACCCACA 181 TCCACAGGCC CAGCGATGGC CACCTCTCTA GCACCGCCAC CGTCCAGAAG CCCCCAGGGG 241 CGCTAACTCT AGTTGAGAGT CAGAAACGTA TGAGCAAACT AAACGCAACC CCTCCCCTGT 301 TAGAGCCTTC TTCCCACAAA CCCCTCACCC CATTGCTACC CATTCCAGAC CAAAGGCCGC 361 GGAAAGTTCC GAGCGAGGTG CACGCGTGAC TCTCTTCGGC TGGCCACGAG CAGCTGCTAG 421 GATAATCTGA TCCTGGGACA AAGGCCCGCC CGCCCGGGCT GCAGCCGCAG GTAAACACGC 481 GCTTTGTTAT GGAAATCAGC GCAGGACAGC CCGGAGCAGA CAACCCACGA TTCCGAGGAA 541 GGAGAGGCAT AGGGGGAGGG GCCTCGGGTG GGGCGATCTG TCATCTGCGC GGCAGCCGCC 601 AGCTGAGCCA CGCGCCTCCC CTTTTCTCAT GTAAATCAGC ACATGCCTTT GCAACTTTCT 661 GCAGCCGGCT TCCCCAAGAC CCACGCACCC CAGGGTGAGG TGTCCTCGGA GCGCATACAA 721 TGGCTCTGAG TTCCTATCTA GCTACCCAAA AGGAAAATCA CCAACTAGGT GGGTAGAAAG 781 GAGGCGGCAG CCGGTTTCCA TCAAAGAAGA AAAGGGCTGT GATCCTACTG CATGACGGGA 841 GTTCGGACCC CCGATCCACT CTGAAGTTTC ACGGGGCGCC CAATGCCGGA GTCTTTTGGG 901 TTCATTACTC CCTTTTGAGG TGTACTGCCC CACAGAAACG CAGAAGGAGG GCTAGGCCCT 961 ACCGGGTTTC CACCCTTGGT TGGGGTTGTG AATTCTTGGA AGGCTCGAAA GTCTTCTAAG 1021 TCTCTAAATT GGAAGCCCAA ATTGCCTGAT TGCATTCAAA TGTGGTGCAC CAATGGTCTG 1081 TGTACAAAAC ATTTAAAAGT GGTGTTGGCC GCCAGATTTA TTGGTGGCCA ATGCTGTAAA 1141 TTGTTAAAGT TCACAGGCAG GCGTTGAGGC GCAAGACGAG C //
Primer: Luc_R Sequence file: RDB14128_A6Djb.seq
>D03343A2_A6Dj_1_Luc_R_G03_21.ab1 1 ACATGGATTA AGGGAACAGG GCGTATCTCT TCATAGCCTT ATGCAGTTGC TCTCCAGCGG 61 TTCCATCTTC CAGCGGATAG AATGGCGCCG GGCCTTTCTT TATGTTTTTG GCGTCTTCCA 121 CCTAGAAGGG CCCAGGAGAC AGGACAGGAA GTTAACTGCA GGCAGAAATG GACCCTCTCC 181 ACATCACTCC CGTCCACGAC AAGAGGGCCG CACACTGACC TCAGTCGCTA TAACACACTC 241 ATCCTTTTCT GCAGACAGAA CATACACCGA CTGGTACTTG GTGTCCTTTG AAGTAGAGTA 301 GACAGACTCT GGCCTTTTTC TGTCAGGAAT CTCCCCACTG AAAGGAAAAT TAGAGAGAAC 361 CACAGTAAAC ACACAGGAAA GTGGTTGAAC GCGGATATAC CCAGAACATG GACCGGTCTC 421 CCTGCCCTCC TTGCCCAGCT CAGGGCACAT ACCCCCTAAG TGTTGGGGCG ATCTTCTCTT 481 CTCCTGCAGA GCTCTGTGAC TGGCACTTGG TGTCACGTTT GCTGTGTGTA TCCCTGACCG 541 TGGCTTCATC TCCCTTGAGG TCTCGAACGA GGTTATAGTC CACAGTGGGG TATCGGACCT 601 TAAAGCTGCT CTTCTCGGCT CCATGGTCCC CGTGAAAGTC CGCCTTCTTG TTGGTGTTCT 661 TGATCTGGGT AGCCCCAATG ATGCTAACAG AAACGTCCTT CTCGCGCTGG CAATTGGCTA 721 GGTTGTTCAT GGTTTCTGTC TCTCCCCCAC AGGGTTCAGG TGGAGGCTGG TGTTTCTGTA 781 GCTTCAGCCG GACGCAGACC ACCACAGCAG CACAGCCCAG CAGCAGCAGG AGGACAAGCA 841 CCACCCCGGC ACACACGGCC ACCCAGGGGA AAGGGCCCGC CCTGGCTCTC CATATGCCTC 901 TCACTGAGGT CCACCACCAT GGGCCCTGGT GGTGGCTCAG GGAGCAGAAA CTGGCAGTTT 961 GGGGGCCCGC CATAGCCCCT GGGCGCACTC ACACATGTAG CGCTGGCCCC TCTGGGTGGG 1021 CAGGTGGCCC CCAATTTAAT GGCAGGATGC ATTGCTCCAC ATCTTGCTGA CAGGGGCGCT 1081 GCAGATTCTT GCTCCGGATG TTAAGACCAA GAATGGGGGG A //
Primer: Reverse2 Sequence file: RDB14128_A6Djc.seq
>D03343A2_A6Dj_1_Reverse2_B11_05.ab1 1 GGGGCCAATG ATTACGGCCA AGCTCGGAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGA 61 GCTCCACCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC CCCGGGCTGC AGGAATTCGA 121 ATCAAGCTTA TCGATTTGTA GTTCATTATA TTTATTTTGG AAAAATATTT TTAAAATGAA 181 TTTCTTCATT AACAAAACAG TAAAAAACTC AAATAACATT TGCACAATAT TCCATAAATA 241 CATTTATATA CAAAAAAATA TAGTCACATA GACCCGAAGT GCCTTTGTAC ATATTTACCA 301 AAATTTAAAT TATAAAAAAA TGAACGTCAC AAAATACTCA AGCTTTACAG ATATCATGAA 361 AAATATTTTT ACAAATCCCA AAAAAATAAC ACACGTTTTC TTTTTCCCGT CTAGGAAAAA 421 AAAAAACACA TCTCAGTATC AAAAAGGACA ATAAAGGCAG TTGTGTTTCT AGTTCAAGGA 481 AAGACTGGCT CATAGTAATC CAAGATAGAC GTGTGGGCAG TGCGTGCTTC CTATATGAAT 541 CAAGTCACTC GTCCATTTAA ATATATACTC TTCAAAGCAA CTGTCCATAG TGCAATGGGA 601 ACAACCAGCA GGCAGTCCAG CGGCAGCCAA AGCCTAGGCT GGCAGTGTCG GGCGCCTCTG 661 CTAACTCTGA GAGAACCAGC TTCGCCTGAA CCTGGTTCTC AGCAGCAGTC CCTGGGTTTC 721 CTCTCCCTTC CTCTCTCAGC AGCATTCATC GGGGCTATAT CCTTGGAATT TTATTTAAGA 781 GAAATGGGGA ATTTTGCCAC ATCGCTTCCA TCTTACACGG CGATCTTTCC GCCCTTCTTG 841 GCCTTTATGA GGATCTCTCT GATTTTTTCT TGCGTCGAGT TTTCCGGTAA GACCTTTTCG 901 GTACTTCGTC CACAAACACA ACTCCTCCGC GCAACTTTTT CGCGGGTTGT TACTTGACTG 961 GGCGACGTAA TCCACGATCT CTTTTTTCCG TCATCGTCTT TCCGGTGCTC AAACAACACC 1021 GCCGCGGGAG TTCACGGCGT TCATCGTCGG AAGACTGCGA CACTGCGTCG TAGATGATCG 1081 GTGTTGGGAG CCAAGG //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Shimojo, H., Oscillatory control of Delta-like1 in cell interactions regulates dynamic gene expression and tissue morphogenesis. Genes Dev. 30 (1): 102-116 (2016). PMID 26728556. [link to RRC of NBRP]