p53 dependent G2 arrest
DNA damaging agents induces accumulation and stimulation of p53 protein,which activate or inhibit several gene expression.
p53 activates p21,14-3-3 sigma and Gadd45 transcription. p21 binds Cyclin B/cdc2 complex to keep it inactive form. 14-3-3 sigma is a cytoplasmic protein that binds to Cyclin B/cdc2 complex to let it stay in cytoplasm, therefore Cyclin B/cdc2 can not act as an active complex. Gadd45 inhibits formation of Cyclin B/cdc2 complex.
p53 also inhibits G2-M transition by repressing the transcription of cdc2 and CyclinB. (Contributed by Dr. Koji Nakade)
Reporters
List of gene resources
| Locus | RDB no. | Type | Name of clone | Running title |
|---|---|---|---|---|
| Trp53 | 1285 | DNA clone | p53-LTR pUC18 (Anti Sense#9) | Expression vector, Antisense p53, cDNA |
| Trp53 | 1284 | DNA clone | p53-LTR pUC18 (Sense#3) | Expression vector, Sense p53, cDNA |
| TP53 | 5507 | DNA clone | pKM2L-phP53 | Promoter Bank clone, Human p53 promoter |
| TP53 | 3187 | Recombinant adenovirus | AxCAhp53KP1065 | Recombinant adenovirus harboring human p53 cDNA (Ser96Pro) |
| TP53 | 3186 | Recombinant adenovirus | AxCAhp53KP1010 | Recombinant adenovirus harboring human p53 cDNA (Gly105Asp) |
| TP53 | 3185 | Recombinant adenovirus | AxCAhp53KP1007 | Recombinant adenovirus harboring human p53 cDNA (Gly374Cys) |
| TP53 | 3184 | Recombinant adenovirus | AxCAhp53KP1094 | Recombinant adenovirus harboring human p53 cDNA (Pro89His) |
| TP53 | 3183 | Recombinant adenovirus | AxCAhp53KP1070 | Recombinant adenovirus harboring human p53 cDNA (Ser240Asn) |
| TP53 | 2807 | Recombinant adenovirus | AxCALNLhp53R273H (forward) | Recombinant adenovirus harboring human p53 cDNA (R273H) |
| TP53 | 2802 | Recombinant adenovirus | AxCALNLhp53.clone 28 (forward) | Recombinant adenovirus harboring human p53 cDNA (deletion) |
| TP53 | 2799 | Recombinant adenovirus | AxCALNLhp53P70 (forward) | Recombinant adenovirus harboring human p53 cDNA (Pro128Thr) |
| TP53 | 2798 | Recombinant adenovirus | AxCALNLhp53P68 (forward) | Recombinant adenovirus harboring human p53 cDNA |
| TP53 | 2795 | Recombinant adenovirus | AxCALNLhp53WT (forward) | Recombinant adenovirus harboring human p53 cDNA |
| TP53 | 2785 | Recombinant adenovirus | AxCAhp53KP1070 (forward) | Recombinant adenovirus harboring human p53 cDNA (Ser240Asn) |
| TP53 | 2784 | Recombinant adenovirus | AxCAhp53KP1065 (forward) | Recombinant adenovirus harboring human p53 cDNA (Ser96Pro) |
| TP53 | 2783 | Recombinant adenovirus | AxCAhp53KP1010 (forward) | Recombinant adenovirus harboring human p53 cDNA (Gly105Asp) |
| TP53 | 2782 | Recombinant adenovirus | AxCAhp53KP1007 (forward) | Recombinant adenovirus harboring human p53 cDNA (Gly374Cys) |
| TP53 | 2771 | Recombinant adenovirus | AxCAhp53.clone 28 (forward) | Recombinant adenovirus harboring human p53 cDNA (deletion) |
| TP53 | 2757 | Recombinant adenovirus | AxCAhp53P70 (forward) | Recombinant adenovirus harboring human p53 cDNA (Pro128Thr) |
| TP53 | 2756 | Recombinant adenovirus | AxCAhp53P68 (forward) | Recombinant adenovirus harboring human p53 cDNA |
| TP53 | 2755 | Recombinant adenovirus | AxCAhp53WT (forward) | Recombinant adenovirus harboring human p53 cDNA |
| TP53 | 2713 | ShuttleVector | pAxCALNLhp53R273H (reverse) | Shuttle vector to produce rAd expressing human p53 (R273H) |
| TP53 | 2712 | ShuttleVector | pAxCALNLhp53R273H (forward) | Shuttle vector to produce rAd expressing human p53 (R273H) |
| TP53 | 2630 | ShuttleVector | pAxCAhp53R273H (reverse) | Shuttle vector to produce rAd expressing human p53 (R273H) |
| TP53 | 2629 | ShuttleVector | pAxCAhp53R273H (forward) | Shuttle vector to produce rAd expressing human p53 (R273H) |
| TP53 | 2509 | ShuttleVector | pAxCAhp53P26 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2508 | ShuttleVector | pAxCAhp53P26 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2507 | ShuttleVector | pAxCAhp53P25 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2506 | ShuttleVector | pAxCAhp53P25 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2505 | ShuttleVector | pAxCAhp53P24 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2504 | ShuttleVector | pAxCAhp53P24 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2503 | ShuttleVector | pAxCAhp53P21 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2502 | ShuttleVector | pAxCAhp53P21 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2501 | ShuttleVector | pAxCAhp53P19 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2500 | ShuttleVector | pAxCAhp53P19 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2499 | ShuttleVector | pAxCAhp53P17 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2498 | ShuttleVector | pAxCAhp53P17 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2497 | ShuttleVector | pAxCAhp53P16 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2496 | ShuttleVector | pAxCAhp53P16 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2495 | ShuttleVector | pAxCAhp53P50 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2494 | ShuttleVector | pAxCAhp53P50 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2493 | ShuttleVector | pAxCAhp53P13 (reverse) | Shuttle vector to generate rAd harboring human p53 |
| TP53 | 2492 | ShuttleVector | pAxCAhp53P13 (forward) | Shuttle vector to produce rAd expressing human p53 |
| TP53 | 2437 | DNA clone | pCAGhp53R273H (Reverse) | Mammalian vector expressing mutant of p53 gene (R273H) |
| TP53 | 2436 | DNA clone | pCAGhp53P70 (Reverse) | Mammalian vector expressing mutant of p53 gene (Pro128Thr) |
| TP53 | 2435 | DNA clone | pCAGhp53P68 (Forward) | Mammalian vector expressing mutant of p53 gene (Ile 162 Val, Gly 266 Asp, frame shift) |
| TP53 | 2434 | DNA clone | pCAGhp53WT (Reverse) | Mammalian vector expressing wild type of p53 gene |
| TP53 | 2433 | DNA clone | pCAGhp53R273H (Forward) | Mammalian vector expressing mutant of p53 gene (R273H) |
| TP53 | 2432 | DNA clone | pCAGhp53clone28 (Forward) | Mammalian vector expressing the deletion mutant of p53 gene |
| TP53 | 2431 | DNA clone | pCAGhp53P70 (Forward) | Mammalian vector expressing mutant of p53 gene (Pro128Thr) |
| TP53 | 2430 | DNA clone | pCAGhp53P68 (Reverse) | Mammalian vector expressing mutant of p53 gene (Ile 162 Val, Gly 266 Asp, frame shift) |
| TP53 | 2429 | DNA clone | pCAGhp53WT (Forward) | Mammalian vector expressing wild type p53 gene |
| TP53 | 1982 | Recombinant Adenovirus | AxCALNLhp53 | Recombinant adenovirus expressing human p53 |
| GADD45B | 5377 | ShuttleVector | pAxCALNLhGADD45B(reverse) | Shuttle vector to generate rAd harboring GADD45B Sugano full-length cDNA clone |
| GADD45B | 5376 | ShuttleVector | pAxCALNLhGADD45B(forward) | Shuttle vector to generate rAd harboring GADD45B Sugano full-length cDNA clone |
| GADD45B | 5029 | ShuttleVector | pAxCALNLhGADD45B(reverse) | Shuttle vector to generate rAd expressing human GADD45B |
| GADD45B | 5028 | ShuttleVector | pAxCALNLhGADD45B(forward) | Shuttle vector to generate rAd expressing human GADD45B |
| GADD45B | 4151 | ShuttleVector | pAxCALNLhGADD45B | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| GADD45B | 3584 | ShuttleVector | pAxCALNLhGADD45B(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDKN1A | 5893 | DNA clone | pKM2L-phP21 | Promoter Bank clone, Human p21 cyclin dependent kinase inhibitor/WAF1/CIP1 promoter |
| CDKN1A | 3576 | ShuttleVector | pAxCALNLhp21(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDKN1A | 1999 | ShuttleVector | pAxCAhp21 | Shuttle vector to generate rAd expressing human CDKI p21 |
| CDK7 | 4997 | ShuttleVector | pAxCALNLhCDK7(reverse) | Shuttle vector to generate rAd expressing human CDK7 |
| CDK7 | 4996 | ShuttleVector | pAxCALNLhCDK7(forward) | Shuttle vector to generate rAd expressing human CDK7 |
| CDK7 | 4113 | ShuttleVector | pAxCALNLhCDK7 | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDK7 | 4087 | ShuttleVector | pAxCALNLhCDK7 | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDK7 | 3539 | ShuttleVector | pAxCALNLhCDK7(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CDK7 | 3503 | ShuttleVector | pAxCALNLhCDK7(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| Cdk7 | 6948 | H3002H05 | Cdk7, NIA/NIH Mouse 15K cDNA Clone (full CDS) | |
| cdc2 | 1570 | DNA clone | pIBI delta Xe-cdc2-33R | Xenopus cdc2 (kinase-deficient) cDNA |
| cdc2 | 1569 | DNA clone | pIBI delta Xe-cdc2 | Xenopus cdc2 cDNA |
| CCNH | 6416 | DNA clone | pCMFlag_hsCCNH | Expression vector of human CCNH |
| CCNH | 4093 | ShuttleVector | pAxCALNLhCyclin H | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CCNH | 3510 | ShuttleVector | pAxCALNLhCyclin H(forward) | Shuttle vector to generate rAd harboring Sugano full-length cDNA clone |
| CCND3 | 4589 | DNA clone | SEREX clone NGO-Br-20 (ID 859) #1 | SEREX clone NGO-Br-20 (ID 859) #1 |
go to Head(GSB0013e,T.M. 2004.04.05)
2018.09.24
